SRR2933451.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933451.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	165048
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10401	6.301803111822016	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	5821	3.526852794338617	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	4256	2.578643788473656	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	2605	1.5783287286122822	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGT	1159	0.7022199602539867	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	931	0.5640783287286123	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	802	0.48591924773399253	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	786	0.4762250981532645	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC	739	0.44774853375987594	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTCAAAGGTCGTATGCCG	515	0.31203043962968346	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	443	0.2684067665164074	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGT	344	0.20842421598565264	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	327	0.1981241820561291	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	279	0.16904173331394504	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	225	0.13632397847898794	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	195	0.11814744801512288	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT	191	0.11572391061994086	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGTCAAAGGTCGTATGC	186	0.11269448887596335	No Hit
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	186	0.11269448887596335	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATCCACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	184	0.11148272017837235	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	180	0.10905918278319034	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	170	0.10300033929523532	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	170	0.10300033929523532	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCACC	20	7.015714E-4	45.000004	28
TCGGGCG	20	7.015714E-4	45.000004	5
CGTCTTT	30	2.1541746E-6	45.000004	1
CACTGGG	25	3.876247E-5	45.000004	3
GACCCGT	50	2.1827873E-11	45.000004	38
ATACTAT	50	2.1827873E-11	45.000004	45
TATGAAG	20	7.015714E-4	45.000004	27
AATGCTA	100	0.0	45.000004	2
GGGAACG	20	7.015714E-4	45.000004	7
ATCCCTG	25	3.876247E-5	45.000004	6
CGGGCTA	30	2.1541746E-6	45.000004	6
AGCGCGG	20	7.015714E-4	45.000004	14
CGAATAT	20	7.015714E-4	45.000004	14
CGGGAAA	25	3.876247E-5	45.000004	6
GGGCACA	20	7.015714E-4	45.000004	7
GCTACGA	20	7.015714E-4	45.000004	10
AGCTACG	20	7.015714E-4	45.000004	9
TATACCG	20	7.015714E-4	45.000004	27
TTCGCGG	30	2.1541746E-6	45.000004	2
GTCGGGC	20	7.015714E-4	45.000004	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content