FastQCFastQC Report
Sat 14 Jan 2017
SRR2933447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749909
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364274.857522712755815No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG125771.67713682593488No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC109931.4659111972252634No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC78781.0505274639989652No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGGTTT29020.38698028694148223No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT24270.3236392682312121No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC22530.300436452956292TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTA16520.22029339559866598No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC16270.21695965777180967No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGCAGGTTTCGTATGCCG10110.1348163577180698No Hit
GAATCTTTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC9940.1325494159958075No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT9800.13068252281276796No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGT7500.10001213480568977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG1500.045.017
ACACCGA207.0317177E-445.010
ATCCGTC253.889504E-545.06
CGTTTTT309350.044.6508871
TACGGCT15850.042.8706637
GCGACCG850.042.35294311
ACGGCTG16050.042.336458
TGATACC15350.042.2149854
TGCTACG1500.042.04
GAATGAT52600.041.9201551
ATGATAC50000.041.8953
GATACCT15550.041.6720285
CGGCTGT15250.041.606569
AATGATA49950.041.5765762
CACCGGT1850.041.35135316
ATACGGC34900.041.3252146
CGACCAC11400.041.2512
ATCCGGC1150.041.086966
CGGCGAC18800.041.0505339
GATACGG35250.041.0425535
Produced by FastQC (version 0.11.5)