##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933446.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	309176
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.18197402126944	31.0	28.0	31.0	25.0	34.0
2	28.137378062980307	31.0	25.0	33.0	16.0	34.0
3	28.06867285947163	31.0	25.0	33.0	16.0	34.0
4	32.980092245193674	35.0	32.0	37.0	28.0	37.0
5	34.264367221259086	35.0	33.0	37.0	32.0	37.0
6	33.377629570212434	35.0	32.0	37.0	28.0	37.0
7	33.924534892747175	35.0	33.0	37.0	30.0	37.0
8	33.33092478070743	35.0	32.0	37.0	28.0	37.0
9	34.98339133697311	37.0	34.0	39.0	29.0	39.0
10	35.08259049861567	37.0	34.0	39.0	28.0	39.0
11	35.42460928403239	37.0	34.0	39.0	30.0	39.0
12	35.428519678112146	37.0	34.0	39.0	30.0	39.0
13	35.489581985665126	37.0	34.0	39.0	30.0	39.0
14	36.27081985665123	38.0	35.0	40.0	30.0	41.0
15	36.4824760007245	38.0	34.0	41.0	30.0	41.0
16	36.5820632908117	38.0	34.0	41.0	31.0	41.0
17	35.6691528449815	38.0	33.0	40.0	27.0	41.0
18	35.41101508525888	38.0	34.0	39.0	27.0	40.0
19	34.67132312986778	37.0	33.0	37.0	27.0	40.0
20	33.4254599322069	35.0	32.0	37.0	26.0	39.0
21	33.98923913887236	35.0	33.0	37.0	27.0	39.0
22	34.405891789789635	35.0	33.0	38.0	28.0	40.0
23	34.603555903433644	35.0	33.0	38.0	29.0	40.0
24	34.17054363857479	35.0	33.0	38.0	27.0	40.0
25	33.30595518410226	35.0	33.0	38.0	24.0	40.0
26	33.16810813258468	35.0	32.0	38.0	24.0	40.0
27	33.88942220612208	35.0	33.0	38.0	25.0	40.0
28	33.55878528734443	35.0	33.0	38.0	24.0	40.0
29	33.3703909747199	35.0	33.0	38.0	23.0	40.0
30	32.62896214453903	35.0	31.0	38.0	21.0	40.0
31	32.630181514735945	35.0	32.0	38.0	20.0	40.0
32	31.79950901751753	35.0	31.0	38.0	15.0	40.0
33	30.71634602944601	35.0	27.0	38.0	10.0	40.0
34	30.029921468678033	35.0	23.0	38.0	8.0	40.0
35	29.25033314358165	35.0	21.0	38.0	7.0	40.0
36	28.699430098067122	35.0	18.0	38.0	7.0	40.0
37	28.286005382047765	35.0	17.0	38.0	7.0	40.0
38	27.928011876730405	34.0	16.0	38.0	7.0	40.0
39	27.705947421533367	34.0	15.0	38.0	7.0	40.0
40	27.293366884881102	33.0	15.0	38.0	7.0	40.0
41	27.038211245374804	33.0	15.0	38.0	7.0	40.0
42	27.13267200558905	33.0	15.0	38.0	7.0	40.0
43	27.002393458741945	33.0	13.0	38.0	7.0	40.0
44	27.054606437757133	34.0	12.0	38.0	7.0	40.0
45	27.08502600460579	34.0	10.0	38.0	7.0	40.0
46	26.831177064196446	33.0	10.0	38.0	7.0	40.0
47	26.550003881284447	33.0	10.0	38.0	7.0	40.0
48	26.53313323155743	33.0	10.0	38.0	7.0	40.0
49	26.727397986907135	33.0	10.0	38.0	7.0	40.0
50	26.66079514580692	33.0	10.0	38.0	7.0	40.0
51	25.521224157114393	31.0	10.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	13.0
10	16.0
11	23.0
12	23.0
13	36.0
14	64.0
15	84.0
16	173.0
17	392.0
18	799.0
19	1450.0
20	2353.0
21	3498.0
22	5039.0
23	7197.0
24	10610.0
25	15125.0
26	19806.0
27	20804.0
28	18355.0
29	16025.0
30	15464.0
31	16325.0
32	17799.0
33	20396.0
34	22731.0
35	25336.0
36	25600.0
37	23831.0
38	17322.0
39	2485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.053108908841565	2.238207364090356	22.322560612725436	45.38612311434264
2	43.372383367402385	21.65012808238673	24.866742567340285	10.110745982870597
3	9.029484824177816	21.813788909876575	59.05115532900355	10.105570936942065
4	8.55499780060548	2.1017155277253083	78.15807177788703	11.185214893782183
5	23.8058581519911	2.5610008538825784	60.45423965637695	13.178901337749371
6	10.26826144332031	18.778301032421663	62.79821202163169	8.155225502626335
7	39.12399410044764	1.0233653323673249	57.4485082930111	2.4041322741739335
8	37.02163169198126	15.109516909462572	44.01732346624576	3.8515279323103995
9	34.76401790565891	2.4555592930887262	42.91859652754418	19.86182627370818
10	25.38360027945248	14.350725800191476	48.43196108365462	11.833712836701425
11	21.778210469117916	12.050741325329263	54.36612156182886	11.804926643723963
12	16.19530623334282	11.005382047765675	55.17601624964421	17.623295469247292
13	14.755026263358086	13.347413770797214	63.05308303361193	8.844476932232775
14	12.205669262814707	20.562721556653816	52.03023520583745	15.201373974694024
15	7.678150956089736	12.792066654591562	66.15196522369135	13.377817165627345
16	9.086086889021141	15.139596863922167	53.25607421015861	22.51824203689808
17	9.762077263435714	16.516159080911844	59.3839754702823	14.337788185370146
18	9.548606618883742	14.542202499547182	58.22379486117939	17.685396020389682
19	10.565503143840402	17.386537118016925	53.78004761042254	18.267912127720134
20	13.100305327709783	15.619582373793566	61.0270525525914	10.253059745905245
21	12.857725049809817	22.77602401221311	53.918156648640256	10.448094289336819
22	11.006675809247806	12.75778197531503	57.39158278779725	18.84395942763992
23	11.551025952855332	21.729370973167388	54.55759826118457	12.162004812792713
24	13.351295055243614	14.558374518073848	53.401622376898594	18.68870804978394
25	9.516586022200947	25.12872926747225	51.47068336481486	13.884001345511942
26	11.539382099516134	13.57899707609905	57.69788081869227	17.18374000569255
27	14.198708826040832	17.36389629207959	52.4067197971382	16.03067508474138
28	10.195810800320853	16.649739953942092	58.459582891298155	14.694866354438895
29	18.958457318808705	12.451160504049472	53.45854788211245	15.131834295029368
30	11.441379667244547	20.869343027919374	56.81100732268999	10.87826998214609
31	19.42130048904184	14.508888141382254	47.43835226537635	18.63145910419955
32	20.633554997800605	21.54565684270448	46.69832069759619	11.122467461898724
33	13.47387895567573	20.091468936786814	44.79713819960152	21.637513907935933
34	21.6177840453334	19.269283514891196	42.528850881051575	16.584081558723835
35	12.951199316893938	24.2809920563045	45.44434238103863	17.32346624576293
36	22.534737495795277	23.182588557973453	38.36196858747121	15.92070535876006
37	18.163764328408412	21.132946929904005	46.56085854011954	14.142430201568038
38	19.014089062540428	27.620837322431235	36.99607990270914	16.368993712319195
39	20.979312753900693	21.885269231764433	38.189251429606436	18.946166584728438
40	15.160620488006831	28.74608637151655	40.779038476466475	15.314254664010143
41	15.872189303180065	28.00443760188372	36.281600124201105	19.841772970735118
42	15.96501668952312	25.088622661526124	42.20605739125935	16.740303257691412
43	19.256345900069864	24.86124408104122	35.56809066680467	20.31431935208425
44	18.528928506740495	22.075775610008538	39.01014309001992	20.38515279323104
45	16.44209123605972	21.886886433617097	38.00747794136673	23.66354438895645
46	23.329107045825033	24.96539188035294	34.57254120630321	17.132959867518824
47	13.983944420006727	21.4001086759645	46.18631459104199	18.429632312986776
48	19.031554842549227	22.634033689548993	34.949672678345024	23.384738789556756
49	15.539045721530778	20.140308432737342	43.17314409915388	21.147501746578
50	17.814772168602996	20.019665174528424	39.88698993453567	22.27857272233291
51	18.13982974098895	18.757600848707533	37.87939555463555	25.22317385566797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	461.5
2	900.0
3	12549.5
4	24199.0
5	18597.0
6	12995.0
7	12946.5
8	12898.0
9	12761.0
10	12624.0
11	11974.5
12	11325.0
13	10511.5
14	9698.0
15	8846.0
16	7994.0
17	7211.0
18	6428.0
19	5894.5
20	5361.0
21	4985.5
22	4610.0
23	4397.5
24	4185.0
25	3989.0
26	3727.0
27	3661.0
28	3646.0
29	3631.0
30	3772.0
31	3913.0
32	4079.5
33	4246.0
34	4894.5
35	5543.0
36	5519.5
37	5496.0
38	5876.5
39	6257.0
40	6903.0
41	7549.0
42	8234.0
43	8919.0
44	9923.5
45	10928.0
46	32641.0
47	54354.0
48	36766.5
49	19179.0
50	17251.5
51	15324.0
52	12330.0
53	9336.0
54	8509.5
55	7683.0
56	7151.5
57	6620.0
58	6014.0
59	5408.0
60	4860.5
61	4313.0
62	3742.0
63	3171.0
64	2822.5
65	2474.0
66	1957.5
67	1441.0
68	1244.0
69	1047.0
70	828.5
71	610.0
72	520.5
73	431.0
74	338.0
75	192.0
76	139.0
77	123.0
78	107.0
79	91.0
80	75.0
81	49.0
82	23.0
83	18.0
84	13.0
85	8.5
86	4.0
87	3.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	309176.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.40395554972653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.2248288237065	39.60352250744075
2	4.889676925503047	4.4402134755612765
3	1.6749708507743144	2.281509061669338
4	1.0093115209355754	1.8330694172954298
5	0.7352292416039684	1.6691157902322868
6	0.5803926479904371	1.5811273194447528
7	0.4798487968517332	1.5250923409992097
8	0.42110946182272346	1.5296028228934557
9	0.37961336884842717	1.5512353672748336
>10	2.4777701885863674	19.02770606597798
>50	0.07649187950182106	2.3308277021526815
>100	0.04146288795425814	3.5781986615417294
>500	0.002144632135565076	0.6624731012524193
>1k	0.004289264271130152	4.467230422605118
>5k	0.0014297547570433842	6.039183989173206
>10k+	0.0014297547570433842	7.8798919544855375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	12667	4.097019173545165	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11610	3.7551427018914794	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	9517	3.0781820063653065	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	9089	2.939749527777059	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT	2993	0.9680570290061326	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC	2366	0.7652599166817605	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	2158	0.6979843196108365	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	2144	0.6934561544233705	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	2093	0.6769606955261729	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA	2009	0.6497917044013766	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGACAATCGT	874	0.28268688384609414	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTAT	585	0.18921261676197376	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTT	582	0.1882422956503739	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATG	440	0.14231376303464693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	390	0.12614174450798252	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	378	0.12226046006158305	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGGACAA	371	0.11999637746785002	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.10738220301705177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	332	0.10738220301705177	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGTGGACAAT	314	0.10156027634745257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.234403705332885E-4	0.0	0.0	0.13131679043651512	0.0
2	3.234403705332885E-4	0.0	0.0	0.9968432219835951	0.0
3	3.234403705332885E-4	0.0	0.0	1.1197505627862447	0.0
4	3.234403705332885E-4	0.0	0.0	1.9930395632261235	0.0
5	3.234403705332885E-4	0.0	0.0	5.495898776101638	0.0
6	3.234403705332885E-4	0.0	0.0	5.803490568478796	0.0
7	3.234403705332885E-4	0.0	0.0	6.648316816311745	0.0
8	3.234403705332885E-4	0.0	0.0	7.6264004968044095	0.0
9	3.234403705332885E-4	0.0	0.0	7.783269076513054	0.0
10	3.234403705332885E-4	0.0	0.0	12.795624498667426	0.0
11	3.234403705332885E-4	0.0	0.0	13.100305327709783	0.0
12	3.234403705332885E-4	0.0	0.0	16.977385049292312	0.0
13	3.234403705332885E-4	0.0	0.0	17.28594716278107	0.0
14	3.234403705332885E-4	0.0	0.0	17.55763707402903	0.0
15	3.234403705332885E-4	0.0	0.0	18.400846120009316	0.0
16	3.234403705332885E-4	0.0	0.0	18.84266566615779	0.0
17	3.234403705332885E-4	0.0	0.0	19.10432892591922	0.0
18	3.234403705332885E-4	0.0	0.0	19.277369524154526	0.0
19	3.234403705332885E-4	0.0	0.0	20.61414857556861	0.0
20	3.234403705332885E-4	0.0	0.0	20.888426009780837	0.0
21	3.234403705332885E-4	0.0	0.0	21.05984940616348	0.0
22	3.234403705332885E-4	0.0	0.0	21.407871244857297	0.0
23	3.234403705332885E-4	0.0	0.0	21.57411959531141	0.0
24	3.234403705332885E-4	0.0	0.0	21.726783450203122	0.0
25	3.234403705332885E-4	0.0	0.0	21.84645638730044	0.0
26	3.234403705332885E-4	0.0	0.0	22.00106088441535	0.0
27	3.234403705332885E-4	0.0	0.0	22.196418868217457	0.0
28	3.234403705332885E-4	0.0	0.0	22.296685383082774	0.0
29	3.234403705332885E-4	0.0	0.0	22.420563044997024	0.0
30	3.234403705332885E-4	0.0	0.0	22.55964240432634	0.0
31	3.234403705332885E-4	0.0	0.0	22.69031231402179	0.0
32	3.234403705332885E-4	0.0	0.0	22.830361994462702	0.0
33	3.234403705332885E-4	0.0	0.0	22.962972546381348	0.0
34	3.234403705332885E-4	0.0	0.0	23.152508603513855	0.0
35	3.234403705332885E-4	0.0	0.0	23.310670944704633	0.0
36	3.234403705332885E-4	0.0	0.0	23.449750304033948	0.0
37	3.234403705332885E-4	0.0	0.0	23.61599865448806	0.0
38	3.234403705332885E-4	0.0	0.0	23.77125003234404	0.0
39	3.234403705332885E-4	0.0	0.0	23.94558439206148	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTG	20	7.0252776E-4	45.0	5
ACCGGTG	40	6.7866495E-9	45.0	17
AGTCCAT	20	7.0252776E-4	45.0	39
GGTGTCG	25	3.884168E-5	45.0	29
TACTTGG	20	7.0252776E-4	45.0	2
CGTGCGG	20	7.0252776E-4	45.0	2
GGACTAC	20	7.0252776E-4	45.0	8
GTTAGTT	20	7.0252776E-4	45.0	35
GGTCACT	20	7.0252776E-4	45.0	36
TACGGGA	25	3.884168E-5	45.0	4
CGCGACC	20	7.0252776E-4	45.0	10
CTACGGG	20	7.0252776E-4	45.0	3
CGGGCGA	50	2.1827873E-11	45.0	6
GCTAGTC	20	7.0252776E-4	45.0	30
AGTTAGT	20	7.0252776E-4	45.0	34
TGTAATA	25	3.884168E-5	45.0	43
CTAGGGA	40	6.7866495E-9	45.0	4
GCCCGCG	25	3.884168E-5	45.0	1
AGTAATC	20	7.0252776E-4	45.0	16
GACCCGT	55	1.8189894E-12	44.999996	38
>>END_MODULE