FastQCFastQC Report
Sat 14 Jan 2017
SRR2933443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398829
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260016.519335354249566No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG87422.191916836538968No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC78971.9800465863816323No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC50281.2606906719421105No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTTTCGT22690.5689155001266207No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC19820.496954835280283No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT16830.42198536214768734No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTA11720.2938602759578667No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTC10650.26703173540539926No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCT7670.19231299629665846No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGCTTTCGTCGTATGCCG6500.16297711550564276No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGT6150.15420142467072354No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT5380.13489490483390124No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCC5350.13414270276233675No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5190.13013095838065938No Hit
GAATCTTTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC5170.1296294903329497No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4800.12035233145032082No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGCTTTCGTCGTATGC4390.11007223647227257No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT4260.10681269416215973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA302.1619235E-645.00000419
CACCCGT302.1619235E-645.00000416
CGGGTTA207.0277415E-445.06
GCACGGG207.0277415E-445.03
TATGAGT207.0277415E-445.012
ATCCGGC551.8189894E-1245.06
CGGTGTC800.045.019
CGTTTTT141700.044.6030351
CGATGAA1600.043.5937519
ACCGGTG1550.043.54838617
CCGGTGT1200.043.12500418
ACGGCTG10050.042.537328
TACGGCT10100.042.3267337
GAATGAT37250.042.281881
TGATACC13050.041.7241364
CGGCTGT10000.041.6249969
ATACGGC23400.041.538466
AATGATA35450.041.5091672
GATACCT13200.041.4204525
TGATACG23700.041.3924034
Produced by FastQC (version 0.11.5)