##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933440.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562250
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.255279679857715	31.0	28.0	31.0	25.0	34.0
2	28.35192530013339	31.0	26.0	33.0	16.0	34.0
3	28.387959092930192	31.0	25.0	33.0	16.0	34.0
4	32.935302801245	35.0	32.0	37.0	28.0	37.0
5	34.14264828812806	35.0	33.0	37.0	30.0	37.0
6	33.323941307247665	35.0	32.0	37.0	28.0	37.0
7	33.36174833259226	35.0	32.0	36.0	28.0	37.0
8	33.26290795909293	35.0	32.0	37.0	28.0	37.0
9	34.48987638950645	37.0	34.0	39.0	28.0	39.0
10	34.33073365940418	37.0	32.0	39.0	27.0	39.0
11	34.65547354379724	37.0	33.0	39.0	27.0	39.0
12	34.74936949755447	37.0	33.0	39.0	27.0	39.0
13	34.86962738995109	37.0	34.0	39.0	27.0	39.0
14	35.66538372610049	38.0	34.0	40.0	27.0	41.0
15	35.915628279235214	38.0	34.0	40.0	27.0	41.0
16	35.923062694530906	38.0	34.0	40.0	30.0	41.0
17	35.158473988439304	37.0	33.0	40.0	27.0	41.0
18	35.05513739439751	37.0	33.0	39.0	27.0	40.0
19	34.530875944864384	37.0	33.0	38.0	27.0	40.0
20	33.88263405958204	35.0	32.0	38.0	26.0	40.0
21	34.47218319253001	35.0	33.0	39.0	27.0	40.0
22	35.07239306358382	36.0	33.0	39.0	30.0	40.0
23	35.34651133837261	36.0	34.0	39.0	30.0	40.0
24	34.92070253445976	36.0	33.0	39.0	28.0	40.0
25	33.844992441084926	35.0	33.0	39.0	24.0	40.0
26	34.06732414406403	35.0	33.0	39.0	25.0	40.0
27	34.66344330813695	36.0	33.0	39.0	27.0	40.0
28	34.38486260560249	36.0	33.0	39.0	25.0	40.0
29	34.44218408181414	36.0	33.0	39.0	25.0	40.0
30	33.75020542463317	35.0	33.0	39.0	23.0	40.0
31	33.76213250333482	35.0	33.0	39.0	23.0	40.0
32	33.39593063583815	36.0	33.0	39.0	21.0	40.0
33	32.90596709648733	36.0	32.0	39.0	16.0	41.0
34	32.49247843485994	36.0	31.0	40.0	12.0	41.0
35	32.34045175633615	37.0	31.0	40.0	10.0	41.0
36	31.910164517563363	36.0	30.0	40.0	9.0	41.0
37	31.859550022232103	37.0	30.0	40.0	8.0	41.0
38	31.436636727434415	36.0	29.0	40.0	8.0	41.0
39	31.152008892841263	36.0	28.0	39.0	8.0	40.0
40	30.934433081369498	35.0	26.0	39.0	8.0	40.0
41	30.440037349933302	35.0	24.0	39.0	8.0	40.0
42	30.867461093819475	36.0	27.0	39.0	8.0	40.0
43	30.799921742996887	36.0	26.0	39.0	8.0	40.0
44	30.94761049355269	36.0	27.0	39.0	7.0	40.0
45	30.999142730102268	36.0	27.0	39.0	7.0	40.0
46	30.769598932859047	35.0	26.0	39.0	7.0	40.0
47	30.564366385060026	35.0	26.0	39.0	7.0	40.0
48	30.426141396176078	35.0	26.0	39.0	7.0	40.0
49	30.724455313472653	35.0	27.0	39.0	7.0	40.0
50	30.63211027123166	35.0	27.0	39.0	7.0	40.0
51	29.358509559804357	34.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	33.0
10	35.0
11	37.0
12	47.0
13	59.0
14	83.0
15	124.0
16	258.0
17	505.0
18	1070.0
19	1956.0
20	2991.0
21	4550.0
22	6161.0
23	8984.0
24	12936.0
25	18351.0
26	23166.0
27	24482.0
28	22981.0
29	22834.0
30	24408.0
31	28195.0
32	33377.0
33	40183.0
34	45753.0
35	53059.0
36	59868.0
37	64693.0
38	52821.0
39	8245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.318363717207646	2.748421520675856	31.787816807469987	33.14539795464651
2	31.193952867941306	20.091596265006668	33.956602934637615	14.757847932414405
3	13.16674077367719	20.19421965317919	52.666251667407735	13.972787905735885
4	11.932592263228102	3.1767007558915075	68.66945309026234	16.221253890618055
5	25.094886616273897	3.6681191640729214	53.142374388617164	18.094619831036017
6	14.663939528679412	18.219831036016007	56.37883503779457	10.737394397510004
7	55.95660293463761	1.3751889728768343	39.91996442863495	2.7482436638506003
8	54.49799911071588	13.764873277012004	28.11133837261005	3.625789239662072
9	50.9693196976434	3.327523343708315	27.24268563806136	18.460471320586926
10	31.453268119164076	19.27558915073366	34.856024899955536	14.415117830146732
11	25.960693641618498	17.461093819475323	41.65691418408181	14.921298354824367
12	19.421253890618054	15.66118274788795	44.02667852378835	20.89088483770565
13	18.93926189417519	16.496042685638063	51.951978657180966	12.61271676300578
14	16.927879057358826	23.064117385504666	42.00675855935971	18.001244997776787
15	12.60738105824811	17.71044908848377	53.55073365940418	16.13143619386394
16	14.885015562472208	18.965762561138284	41.58221431747443	24.567007558915073
17	15.560871498443754	19.68216985326812	47.991107158737215	16.76585148955091
18	15.185771453979546	17.980435749221876	46.22249888839484	20.611293908403734
19	15.384259670964873	21.141129390840373	42.893552690084476	20.58105824811027
20	17.931702979101825	19.530280124499775	49.02676745220098	13.511249444197421
21	17.88012449977768	25.67309915518008	42.52129835482437	13.925477990217875
22	15.790484659848822	16.190662516674077	46.425255669186306	21.593597154290798
23	16.024544241885284	24.758381502890174	44.180880391285015	15.036193863939529
24	17.269008448199198	19.222943530457982	41.89275233437083	21.61529568697199
25	14.064739884393063	29.092218763895065	40.12770120053357	16.7153401511783
26	16.14370831480658	17.610493552690084	45.31133837261005	20.934459759893286
27	18.81760782570031	21.11747443308137	40.4361049355269	19.628812805691418
28	13.687683414851046	21.382481102712315	46.73348154735438	18.19635393508226
29	22.63245887060916	17.403823921742998	40.76033792796799	19.203379279679858
30	18.517207647843488	24.64099599822143	42.45406847487772	14.387727879057358
31	19.297109826589594	21.701378390395732	37.760960426856386	21.240551356158292
32	21.24730991551801	23.491329479768787	37.488128056914185	17.773232547799022
33	17.357936860827035	23.354201867496666	35.27416629613162	24.013694975544688
34	23.310626945309025	24.441618497109825	36.10564695420187	16.14210760337928
35	20.147976878612717	22.478257003112496	37.12850155624722	20.245264562027568
36	20.810137839039573	27.19502000889284	31.806669630947088	20.1881725211205
37	20.562027567807913	23.30600266785238	35.70475767007559	20.427212094264117
38	18.440373499333038	30.37332147621165	31.709737661182746	19.476567363272565
39	20.246153846153845	26.574655402401063	31.894886616273897	21.284304135171187
40	18.69613161405069	25.642152067585595	37.8150289017341	17.846687416629614
41	17.965317919075144	28.597243219208536	30.64686527345487	22.790573588261452
42	17.502356602934636	22.669275233437084	41.294619831036016	18.53374833259226
43	20.82596709648733	22.564339706536238	31.57901289461983	25.0306803023566
44	22.0188528234771	22.87896843041352	34.236193863939526	20.86598488216985
45	17.74299688750556	22.995286794130728	34.65042240995998	24.611293908403738
46	23.352245442418855	25.643041351711872	30.96540684748777	20.039306358381502
47	16.246153846153845	23.041707425522453	41.58648288128057	19.12565584704313
48	20.418674966651846	26.270875944864386	30.811916407292127	22.49853268119164
49	17.496487327701203	21.542374388617162	39.37483325922632	21.586305024455314
50	19.970653623832817	21.46536238328146	34.684037349933305	23.879946642952422
51	19.945931525122276	21.86162738995109	32.47398843930635	25.718452645620278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	667.5
2	1269.0
3	13690.0
4	26111.0
5	19797.5
6	13484.0
7	12984.5
8	12485.0
9	12162.0
10	11839.0
11	11240.5
12	10642.0
13	10080.5
14	9519.0
15	8833.5
16	8148.0
17	7666.5
18	7185.0
19	6608.5
20	6032.0
21	5773.0
22	5514.0
23	5449.0
24	5384.0
25	5375.5
26	5911.0
27	6455.0
28	7069.0
29	7683.0
30	8681.5
31	9680.0
32	10663.0
33	11646.0
34	12722.0
35	13798.0
36	14564.5
37	15331.0
38	16157.0
39	16983.0
40	18589.0
41	20195.0
42	21315.0
43	22435.0
44	24760.5
45	27086.0
46	62209.5
47	97333.0
48	68348.5
49	39364.0
50	36442.5
51	33521.0
52	28942.5
53	24364.0
54	22578.5
55	20793.0
56	19689.5
57	18586.0
58	16780.0
59	14974.0
60	13270.0
61	11566.0
62	10364.5
63	9163.0
64	7774.5
65	6386.0
66	5313.5
67	4241.0
68	3545.0
69	2849.0
70	2435.5
71	2022.0
72	1580.0
73	1138.0
74	964.0
75	607.0
76	424.0
77	297.5
78	171.0
79	136.5
80	102.0
81	86.5
82	71.0
83	52.0
84	33.0
85	22.0
86	11.0
87	9.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.22516570127406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85661533206701	33.74552530809913
2	5.259807731146742	4.336728905467336
3	2.3240805534995723	2.8743181776338576
4	1.5523112505380707	2.559771540935357
5	1.282942471731985	2.64447579911766
6	1.1622998789384957	2.8749603062286155
7	1.0538862862549268	3.0412645746811786
8	0.9173433894962564	3.0254106589561247
9	0.7580638669006634	2.812617767261557
>10	3.7400820694128796	22.415467391412943
>50	0.05850943765044504	1.6547820286232842
>100	0.027944806041389963	2.2855180481967086
>500	8.732751887934364E-4	0.2883673240301746
>1k	0.0034931007551737454	3.9192395418907564
>5k	0.0	0.0
>10k+	0.0017465503775868727	11.521552627465285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	20030	3.5624722098710535	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	16754	2.9798132503334815	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	15059	2.678345931525122	RNA PCR Primer, Index 36 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12164	2.16345042240996	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT	3808	0.6772787905735883	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC	3550	0.6313917296576256	No Hit
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	3238	0.5759004001778568	RNA PCR Primer, Index 15 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT	3093	0.5501111605157848	RNA PCR Primer, Index 15 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT	3084	0.5485104490884838	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTA	2815	0.5006669630947087	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGACAATCTCGT	1167	0.20755891507336596	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTT	1018	0.18105824811027124	RNA PCR Primer, Index 36 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTAT	882	0.15686971987550022	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATG	720	0.12805691418408183	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07309915518008003	0.0
2	0.0	0.0	0.0	0.7674522009782125	0.0
3	0.0	0.0	0.0	0.8754112939084038	0.0
4	0.0	0.0	0.0	1.5686971987550022	0.0
5	0.0	0.0	0.0	4.7514450867052025	0.0
6	0.0	0.0	0.0	5.0262338817252115	0.0
7	0.0	0.0	0.0	5.713828368163628	0.0
8	0.0	0.0	0.0	6.511516229435305	0.0
9	0.0	0.0	0.0	6.6342374388617165	0.0
10	0.0	0.0	0.0	10.889461983103601	0.0
11	0.0	0.0	0.0	11.076745220097822	0.0
12	0.0	0.0	0.0	14.762472209871055	0.0
13	0.0	0.0	0.0	15.00578034682081	0.0
14	0.0	0.0	0.0	15.231302801244997	0.0
15	0.0	0.0	0.0	15.905202312138728	0.0
16	0.0	0.0	0.0	16.237261004891064	0.0
17	0.0	0.0	0.0	16.39626500666963	0.0
18	0.0	0.0	0.0	16.525033348154736	0.0
19	0.0	0.0	0.0	17.47229879946643	0.0
20	0.0	0.0	0.0	17.671320586927525	0.0
21	0.0	0.0	0.0	17.80168963983993	0.0
22	0.0	0.0	0.0	18.072387727879057	0.0
23	0.0	0.0	0.0	18.21556247220987	0.0
24	0.0	0.0	0.0	18.33952867941307	0.0
25	0.0	0.0	0.0	18.436816362827923	0.0
26	0.0	0.0	0.0	18.565762561138282	0.0
27	0.0	0.0	0.0	18.76620720320142	0.0
28	0.0	0.0	0.0	18.871320586927524	0.0
29	0.0	0.0	0.0	18.999911071587373	0.0
30	0.0	0.0	0.0	19.137394397510004	0.0
31	0.0	0.0	0.0	19.26313917296576	0.0
32	0.0	0.0	0.0	19.392263228101378	0.0
33	0.0	0.0	0.0	19.51783014673188	0.0
34	0.0	0.0	0.0	19.70155624722099	0.0
35	0.0	0.0	0.0	19.856113828368162	0.0
36	0.0	0.0	0.0	19.999822143174743	0.0
37	0.0	0.0	0.0	20.14939973321476	0.0
38	0.0	0.0	0.0	20.3121387283237	0.0
39	7.114273010226768E-4	0.0	0.0	20.53179190751445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAAAT	20	7.0302084E-4	45.0	45
TCGAGCG	20	7.0302084E-4	45.0	32
CGTTTTT	10675	0.0	44.367683	1
GATACCT	2300	0.0	42.847824	5
TGATACC	2325	0.0	42.48387	4
CCGATGA	470	0.0	42.12766	18
TACCTGT	2380	0.0	41.97479	7
CGATGAA	490	0.0	41.785713	19
ACGGCTG	2105	0.0	41.686462	8
CGGCTGT	2125	0.0	41.611763	9
TACGGCT	2110	0.0	41.587677	7
ATACCTG	2400	0.0	41.53125	6
CGACCAC	1715	0.0	40.53936	12
CGGGCAC	50	1.0786607E-9	40.5	6
GAATGAT	7610	0.0	40.239815	1
ATGATAC	7335	0.0	40.092022	3
CGACCCT	45	1.9252184E-8	40.000004	12
GACCGAT	175	0.0	39.857143	9
AATGATA	7270	0.0	39.80055	2
ATACGGC	4885	0.0	39.749233	6
>>END_MODULE