##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933439.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383671
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.223597822092366	31.0	26.0	31.0	16.0	33.0
2	28.43693685475316	31.0	28.0	33.0	16.0	34.0
3	28.682433647578264	31.0	28.0	33.0	16.0	34.0
4	31.69711810379205	35.0	28.0	35.0	19.0	37.0
5	33.4765645566123	35.0	33.0	37.0	28.0	37.0
6	32.2476027638263	35.0	32.0	37.0	17.0	37.0
7	31.70314149362344	33.0	30.0	35.0	25.0	37.0
8	32.52779595017606	35.0	32.0	35.0	25.0	37.0
9	32.54103124812665	34.0	31.0	37.0	25.0	39.0
10	31.218327681789866	35.0	27.0	38.0	15.0	39.0
11	32.00927878312408	35.0	27.0	39.0	17.0	39.0
12	32.89774311845305	35.0	31.0	39.0	23.0	39.0
13	33.03060955871046	35.0	31.0	39.0	24.0	39.0
14	33.094104063116575	36.0	31.0	39.0	18.0	41.0
15	33.795752089681	36.0	32.0	39.0	25.0	41.0
16	34.01558627052866	36.0	32.0	40.0	25.0	41.0
17	31.93326313429996	34.0	27.0	39.0	18.0	41.0
18	32.760075689848854	35.0	30.0	39.0	21.0	39.0
19	32.71326996306732	36.0	30.0	37.0	19.0	39.0
20	32.40805794547933	34.0	30.0	37.0	24.0	39.0
21	32.820369535357116	35.0	31.0	37.0	24.0	39.0
22	33.24831170455938	35.0	31.0	38.0	25.0	39.0
23	34.198307925279735	35.0	33.0	39.0	27.0	40.0
24	32.53015734835314	35.0	31.0	37.0	22.0	39.0
25	31.564280333931936	35.0	29.0	37.0	18.0	39.0
26	31.23977053256566	34.0	27.0	37.0	20.0	39.0
27	32.728045643272495	35.0	31.0	38.0	22.0	40.0
28	32.65267116878784	35.0	31.0	38.0	22.0	40.0
29	32.945140497978734	35.0	31.0	38.0	22.0	40.0
30	31.753755170445512	35.0	30.0	38.0	18.0	40.0
31	32.08841429245369	35.0	30.0	38.0	21.0	40.0
32	32.07047965574672	35.0	30.0	38.0	19.0	40.0
33	32.10524642206474	35.0	30.0	38.0	16.0	40.0
34	31.27478751326006	35.0	30.0	38.0	12.0	40.0
35	30.899914249448095	35.0	29.0	38.0	10.0	40.0
36	30.381592562377662	35.0	27.0	38.0	8.0	40.0
37	30.49552350842258	35.0	27.0	38.0	8.0	40.0
38	30.275405230001745	35.0	25.0	38.0	8.0	40.0
39	29.841828024531434	34.0	25.0	38.0	8.0	40.0
40	29.67699669769151	34.0	24.0	38.0	8.0	40.0
41	29.22152052148846	34.0	23.0	38.0	7.0	40.0
42	29.832108759848932	34.0	24.0	38.0	8.0	40.0
43	29.875898882115145	35.0	24.0	38.0	8.0	40.0
44	29.894221872385454	35.0	25.0	38.0	7.0	40.0
45	30.093900763935768	35.0	25.0	38.0	7.0	40.0
46	29.833740366094908	35.0	24.0	38.0	7.0	40.0
47	29.79120392211035	35.0	24.0	38.0	7.0	40.0
48	29.5589841296319	34.0	24.0	38.0	7.0	40.0
49	29.422390537726333	34.0	24.0	38.0	7.0	40.0
50	29.60672555392511	34.0	24.0	38.0	7.0	40.0
51	27.879933588934268	32.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	19.0
11	22.0
12	22.0
13	67.0
14	79.0
15	200.0
16	407.0
17	791.0
18	1327.0
19	2109.0
20	3062.0
21	4280.0
22	5985.0
23	8090.0
24	11212.0
25	14802.0
26	18466.0
27	19862.0
28	20303.0
29	21286.0
30	23814.0
31	27243.0
32	31273.0
33	35213.0
34	38880.0
35	40969.0
36	32233.0
37	17693.0
38	3880.0
39	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.736448676079245	2.7502730203742267	34.51003594225261	36.00324236129392
2	34.82045815294875	13.648933591540668	36.44554839954023	15.08505985597035
3	14.213479778247509	13.476650567804185	58.21289594470262	14.096973709245683
4	12.818013349979541	3.2527868929369173	67.29906612696816	16.630133630115385
5	21.155364882933554	3.726109088255302	57.47424225443153	17.644283774379613
6	15.106692973928181	12.843295427592913	59.89689082573351	12.1531207727454
7	56.74653544312706	1.4335198646757248	39.029533115612075	2.7904115765851474
8	57.408300340656446	9.545157178937162	29.307401393381312	3.7391410870250814
9	52.817127174063195	3.2624305720265543	29.50626969460814	14.414172559302111
10	29.054580617247588	20.26814640668698	36.67126261823281	14.006010357832622
11	23.4737574640773	18.287803873631315	44.336684294617	13.901754367674387
12	19.045744922081678	16.4698400452471	46.098349888315774	18.386065144355452
13	17.91821638852037	17.115445264301968	52.148846277148905	12.817492070028749
14	16.184699912164326	20.14460305834947	45.44336163014666	18.227335399339538
15	12.80133239155422	18.14575508704072	53.30895480763467	15.743957713770392
16	14.91017043247991	17.282776128505933	44.993757672589275	22.81329576642488
17	15.601908927179798	18.1587870858105	49.31907806427903	16.920225922730673
18	15.238837441453745	16.44742500736308	48.58589781349124	19.72783973769193
19	15.794261229021739	19.855553325635768	45.29792452387593	19.05226092146657
20	17.764699443012372	18.632109281128884	49.896916889731045	13.706274386127697
21	18.02950965801429	22.654044741458176	44.75136249547138	14.565083105056154
22	15.94543241475118	16.679655225440545	47.487560957174246	19.887351402634028
23	15.651430522504958	23.05178134391184	45.732150722884974	15.564637410698229
24	16.072364082768832	18.440278259237733	45.07194966520795	20.415407992785486
25	14.291411130890788	24.178267317571564	43.43565189967446	18.094669651863185
26	15.685053079330991	18.409783382116448	46.79764694230213	19.107516596250434
27	16.644729468737538	20.491514865600998	43.99185760716865	18.87189805849282
28	13.850408292521458	20.489951025748624	48.53350917843674	17.126131503293184
29	19.956160356138465	17.461835791602702	44.14302879289808	18.438975059360754
30	16.99893919530014	22.600352906526684	45.135806459179875	15.264901438993302
31	17.851753194794497	20.941640103109176	41.537410958868406	19.66919574322792
32	19.226107785055426	22.609214665690136	41.34010649749394	16.824571051760493
33	16.67574562580961	21.93519968931715	39.40928555976344	21.979769125109794
34	21.15797128268751	23.259250764326726	39.16298078301461	16.419797169971147
35	19.042356602401536	22.651177701728827	38.81294129605834	19.493524399811296
36	19.845127726619943	25.799447964532114	35.25390243203161	19.101521876816335
37	19.357209692679405	23.727099520161804	37.42425150715066	19.491439280008134
38	17.991716861581928	28.593247860797398	35.083183248147506	18.33185202947317
39	18.561736487772077	26.119513854317894	35.15042836179956	20.16832129611047
40	18.392320503764946	24.84759077438743	39.22006093762625	17.540027784221376
41	17.660704092829533	26.177636568831108	34.59370137435459	21.567957963984767
42	17.295808127275713	22.61651258500121	41.249403786056284	18.838275501666793
43	19.63974342600822	22.319383013050242	34.985964537324946	23.054909023616588
44	21.19889175882462	22.86959400111033	36.46822407739965	19.46329016266541
45	17.609879297627394	23.222761167771345	36.94754099215213	22.21981854244913
46	20.746160121562486	25.26226897524181	34.16677309465662	19.82479780853909
47	16.4283982891592	23.094526299876716	41.462346645954476	19.014728765009608
48	19.415332407192622	25.299540491723377	34.045836146073064	21.239290955010933
49	17.206408615715027	21.940673128800455	39.34256172606217	21.510356529422346
50	18.943574051726607	21.773863544547282	36.289425054278276	22.993137349447835
51	19.20890554667932	22.050141918466604	35.076927888738005	23.664024646116076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	367.0
2	667.0
3	13695.5
4	26724.0
5	18437.5
6	10151.0
7	9804.5
8	9458.0
9	9176.0
10	8894.0
11	8432.0
12	7970.0
13	7387.0
14	6804.0
15	6275.0
16	5746.0
17	5318.0
18	4890.0
19	4437.5
20	3985.0
21	3770.5
22	3556.0
23	3568.0
24	3580.0
25	3567.5
26	3834.0
27	4113.0
28	4516.0
29	4919.0
30	5759.0
31	6599.0
32	7313.0
33	8027.0
34	8780.0
35	9533.0
36	10238.5
37	10944.0
38	11552.5
39	12161.0
40	13361.5
41	14562.0
42	15375.5
43	16189.0
44	17686.5
45	19184.0
46	31897.0
47	44610.0
48	36935.5
49	29261.0
50	27299.0
51	25337.0
52	21764.0
53	18191.0
54	16305.5
55	14420.0
56	13342.0
57	12264.0
58	11066.5
59	9869.0
60	8812.0
61	7755.0
62	7012.5
63	6270.0
64	5389.5
65	4509.0
66	3770.5
67	3032.0
68	2605.0
69	2178.0
70	1826.0
71	1474.0
72	1184.5
73	895.0
74	762.5
75	499.0
76	368.0
77	258.0
78	148.0
79	124.0
80	100.0
81	71.5
82	43.0
83	32.5
84	22.0
85	15.0
86	8.0
87	6.0
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	383671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.411564753130946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57764377735542	35.34167679519608
2	6.605010029488256	5.866776612393941
3	3.112897979170043	4.147460105154025
4	2.1179491364393837	3.7624574086726184
5	1.6115881336195732	3.5786575375811562
6	1.283912792918038	3.4212345684031593
7	1.0425338389807537	3.2410391368056755
8	0.868606004535815	3.0860921452320564
9	0.7357323513406746	2.9407522466285694
>10	2.9628872379438085	18.55189445982773
>50	0.04599545075025578	1.419829952645635
>100	0.028672488779380226	2.6579365275703695
>500	0.0017920305487112642	0.6006156600877649
>1k	0.00298671758118544	2.5539430033855623
>5k	0.0011946870324741762	4.159740967392294
>10k+	5.973435162370881E-4	4.669892873023377
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17603	4.5880454868885066	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	9170	2.3900685743775263	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	6510	1.6967662398252668	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	4151	1.0819165378670788	RNA PCR Primer, Index 36 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT	1692	0.4410028383693321	No Hit
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1566	0.4081622014694882	RNA PCR Primer, Index 15 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC	1111	0.2895710126644964	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT	1107	0.28852845276291406	RNA PCR Primer, Index 15 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTA	868	0.22623549864336895	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGACAATCTCGTATGCCG	709	0.1847937425554707	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT	687	0.1790596630967678	No Hit
GAATCTTTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	447	0.1165060690018271	RNA PCR Primer, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12588910811606818	0.0
2	0.0	0.0	0.0	0.8072020038001309	0.0
3	0.0	0.0	0.0	0.9901712665278325	0.0
4	0.0	0.0	0.0	1.5122331372451918	0.0
5	0.0	0.0	0.0	3.0294184340229	0.0
6	0.0	0.0	0.0	3.2947499289756066	0.0
7	0.0	0.0	0.0	3.6604278144556144	0.0
8	0.0	0.0	0.0	4.20985688258951	0.0
9	0.0	0.0	0.0	4.350602469303127	0.0
10	0.0	0.0	0.0	7.492096092746129	0.0
11	0.0	0.0	0.0	7.923194612050429	0.0
12	0.0	0.0	0.0	10.367476301310237	0.0
13	0.0	0.0	0.0	10.59058412024886	0.0
14	0.0	0.0	0.0	10.736281866494991	0.0
15	0.0	0.0	0.0	11.158257986660447	0.0
16	0.0	0.0	0.0	11.423068201662362	0.0
17	0.0	0.0	0.0	11.663638898952488	0.0
18	0.0	0.0	0.0	11.899518076685494	0.0
19	0.0	0.0	0.0	12.670491123905638	0.0
20	0.0	0.0	0.0	12.930088539399643	0.0
21	0.0	0.0	0.0	13.19359555452458	0.0
22	0.0	0.0	0.0	13.585858717494936	0.0
23	0.0	0.0	0.0	13.835291173948514	0.0
24	0.0	0.0	0.0	14.046148914043542	0.0
25	0.0	0.0	0.0	14.226251137041892	0.0
26	0.0	0.0	0.0	14.413129999400528	0.0
27	0.0	0.0	0.0	14.633631418585194	0.0
28	0.0	0.0	0.0	14.792882443551898	0.0
29	0.0	0.0	0.0	14.984192185492258	0.0
30	0.0	0.0	0.0	15.182278566792903	0.0
31	2.606399753955863E-4	0.0	0.0	15.370199989053122	0.0
32	2.606399753955863E-4	0.0	0.0	15.55395117170701	0.0
33	2.606399753955863E-4	0.0	0.0	15.723627795689536	0.0
34	2.606399753955863E-4	0.0	0.0	15.921453537014786	0.0
35	2.606399753955863E-4	0.0	0.0	16.1273591175773	0.0
36	2.606399753955863E-4	0.0	0.0	16.312413500108164	0.0
37	2.606399753955863E-4	0.0	0.0	16.50893604155644	0.0
38	2.606399753955863E-4	0.0	0.0	16.705719222980104	0.0
39	0.0013031998769779316	0.0	0.0	16.957236799236846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	40	6.7939254E-9	45.0	17
ACGTTTG	20	7.0274057E-4	45.0	1
GCGCGAC	20	7.0274057E-4	45.0	9
GGATACG	20	7.0274057E-4	45.0	8
CACTCGA	20	7.0274057E-4	45.0	33
CGCTATG	20	7.0274057E-4	45.0	24
CTACGGG	25	3.8859303E-5	45.0	3
TCGCTAT	20	7.0274057E-4	45.0	23
GATTACG	20	7.0274057E-4	45.0	9
CGTTTTT	8925	0.0	44.319324	1
GATACCT	1135	0.0	43.414097	5
TGATACC	1155	0.0	42.857143	4
ACGGCTG	925	0.0	42.567566	8
TACGGCT	935	0.0	42.112297	7
CGATGAA	155	0.0	42.09677	19
ACCGGTG	70	0.0	41.785713	17
ATGATAC	3250	0.0	41.746155	3
GCGACTG	135	0.0	41.666664	11
ATACCTG	1175	0.0	41.553192	6
GAATGAT	3435	0.0	41.397377	1
>>END_MODULE