##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236619
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.12014250757547	31.0	25.0	31.0	16.0	33.0
2	28.148897594867698	31.0	28.0	33.0	16.0	34.0
3	28.393252443802062	31.0	28.0	33.0	16.0	34.0
4	31.590848579361758	35.0	28.0	35.0	19.0	37.0
5	33.53368495344837	35.0	33.0	37.0	28.0	37.0
6	32.08916443734442	35.0	32.0	37.0	17.0	37.0
7	31.95426825402863	35.0	31.0	35.0	25.0	37.0
8	32.42759879806778	35.0	32.0	35.0	25.0	37.0
9	32.8054213736006	34.0	31.0	37.0	25.0	39.0
10	31.55022631318702	35.0	28.0	39.0	15.0	39.0
11	32.173215168688905	35.0	27.0	39.0	17.0	39.0
12	33.05556189486052	35.0	31.0	39.0	23.0	39.0
13	33.16862973810218	35.0	31.0	39.0	23.0	39.0
14	33.38621581529801	36.0	31.0	39.0	19.0	41.0
15	33.869507520528785	36.0	32.0	39.0	24.0	41.0
16	34.11373135716067	36.0	32.0	40.0	25.0	41.0
17	31.95726040596909	34.0	27.0	39.0	18.0	41.0
18	32.748194354637626	35.0	30.0	39.0	21.0	39.0
19	32.565220882515774	36.0	30.0	37.0	19.0	39.0
20	32.210418436389304	34.0	30.0	36.0	23.0	39.0
21	32.74656726636491	35.0	31.0	37.0	24.0	39.0
22	33.08889818653616	35.0	31.0	38.0	25.0	39.0
23	33.96138940659879	35.0	32.0	38.0	27.0	40.0
24	32.40486182428292	35.0	31.0	37.0	22.0	39.0
25	31.616505859630884	35.0	30.0	37.0	18.0	39.0
26	30.914398252042314	33.0	26.0	37.0	20.0	39.0
27	32.582108790925496	35.0	31.0	38.0	22.0	39.0
28	32.38033294029643	35.0	31.0	38.0	21.0	39.0
29	32.70653244244968	35.0	31.0	38.0	22.0	40.0
30	31.695658421344017	35.0	30.0	38.0	18.0	40.0
31	32.017936006829544	35.0	30.0	37.0	21.0	40.0
32	31.836547360947346	35.0	30.0	37.0	18.0	40.0
33	31.869106031214738	35.0	30.0	38.0	16.0	40.0
34	31.18830694069369	35.0	30.0	38.0	12.0	40.0
35	30.67633621983019	35.0	28.0	38.0	10.0	40.0
36	30.149941467084215	35.0	26.0	38.0	8.0	40.0
37	30.31514798050875	35.0	27.0	38.0	8.0	40.0
38	30.161571978581602	35.0	25.0	38.0	8.0	40.0
39	29.778077838212486	35.0	25.0	38.0	8.0	40.0
40	29.493506438620738	34.0	23.0	38.0	8.0	40.0
41	29.163207519260922	34.0	23.0	38.0	8.0	40.0
42	29.459071334085596	34.0	23.0	38.0	8.0	40.0
43	29.50682320523711	35.0	23.0	38.0	7.0	40.0
44	29.40354324885153	35.0	23.0	38.0	7.0	40.0
45	29.80132618259734	35.0	23.0	38.0	7.0	40.0
46	29.39720817009623	35.0	23.0	38.0	7.0	40.0
47	29.39226351222852	35.0	23.0	38.0	7.0	40.0
48	29.155600353310597	34.0	23.0	38.0	7.0	40.0
49	29.076794340268535	34.0	23.0	37.0	7.0	40.0
50	29.247702001952504	34.0	23.0	38.0	7.0	40.0
51	27.37642370223862	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	13.0
11	16.0
12	17.0
13	30.0
14	68.0
15	110.0
16	229.0
17	489.0
18	860.0
19	1286.0
20	1970.0
21	2683.0
22	3720.0
23	4917.0
24	6707.0
25	8986.0
26	11797.0
27	13024.0
28	13074.0
29	13790.0
30	15258.0
31	17714.0
32	19995.0
33	22183.0
34	23974.0
35	24422.0
36	17974.0
37	9302.0
38	1961.0
39	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.961909229605403	2.42076925352571	32.90817728077627	37.70914423609263
2	37.24722021477565	15.723589398991628	33.78807280903055	13.24111757720217
3	12.95669409472612	15.41127297469772	58.437403589737094	13.19462934083907
4	11.563314864824887	2.812960920298032	70.17906423406404	15.444659980813036
5	21.591250068675805	3.375891200622097	57.99153914098192	17.04131958972018
6	13.479052823315119	14.084245136696547	60.58346962838994	11.853232411598393
7	51.64927584006356	1.1943250542010575	44.12283037287792	3.033568732857463
8	51.89016942849053	11.06081929177285	32.80928412342204	4.239727156314581
9	47.83554997696719	2.750413111373136	32.85154615647941	16.562490755180267
10	28.137216368930645	19.194992794323365	38.66680190517245	14.000988931573543
11	24.107531516911152	16.24975171055579	46.19916405698613	13.443552715546932
12	20.017411957619633	13.75586913984084	47.75905569713337	18.46766320540616
13	17.6034046293831	15.03429563982605	54.70186248779684	12.66043724299401
14	15.104873235031842	19.0838436473825	47.044827338463946	18.76645577912171
15	11.52865999771785	15.743875174859163	56.66831488595591	16.059149941467084
16	13.57033881471902	15.3795764499047	47.05285712474485	23.997227610631437
17	13.87589331372375	16.76915209683077	51.90791948237462	17.44703510707086
18	13.661624806122925	15.830934962957327	50.606248864207856	19.90119136671189
19	14.328942308098675	18.831116689699474	46.84281482044975	19.9971261817521
20	16.65800294988991	17.60213676839138	52.8199341557525	12.919926125966215
21	17.601714148060807	22.048102646025892	45.720757842776784	14.62942536313652
22	14.697889856689446	15.819101593701266	49.131726530836495	20.351282018772796
23	14.810729484952603	22.499883779409092	46.5503615516928	16.1390251839455
24	16.64405647898098	16.078590476673472	46.643338024419	20.63401501992655
25	13.550898279512635	23.116064221385436	44.87382670030725	18.459210798794686
26	13.576678119677627	16.76196755121102	50.32731944602927	19.334034883082087
27	15.095152967428652	19.602398792996336	45.66793030145509	19.63451793811993
28	11.93817909804369	18.778289148377773	51.693650974773796	17.589880778804744
29	17.020188573191504	16.01308432543456	48.794052886708165	18.172674214665772
30	13.963798342483063	23.493041556257104	46.77054674392166	15.77261335733817
31	17.435624358145375	17.017230230877487	44.059437323291874	21.487708087685267
32	19.487023442749738	21.73325049974854	44.32103930791695	14.458686749584777
33	17.059069643604275	20.24562693612939	40.5918375109353	22.103465909331035
34	20.48863362620922	19.361927824899944	43.97322277585485	16.176215773035977
35	17.293201306742063	23.832405681707726	39.60417379838474	19.27021921316547
36	18.819283320443414	20.638241223232285	39.3117205296278	21.230754926696505
37	16.651663644931304	24.66961655657407	43.02190441173363	15.656815386760995
38	18.0712453353281	25.360178176731367	35.87370414041138	20.69487234752915
39	21.640274027022343	21.630976379749725	40.25965793110443	16.469091662123496
40	20.87448598802294	24.013287183193235	38.109788309476414	17.002438519307407
41	23.550940541545692	21.977525050820095	35.92822216305537	18.54331224457884
42	19.343332530354708	21.268368136117555	40.82554655374251	18.562752779785225
43	21.20328460520922	20.69487234752915	36.931522827837156	21.170320219424475
44	20.174204100262447	22.423389499575265	37.42007192998027	19.98233447018202
45	17.377302752526212	20.520752771332816	37.719287124026394	24.38265735211458
46	21.22187989975446	24.434217032444565	36.31027094189393	18.033632125907047
47	15.316183400318655	22.110650454950786	44.08986598709318	18.483300157637384
48	20.65894961943039	22.117835000570537	35.48024461264734	21.742970767351736
49	17.956715225742652	19.337838466057246	41.44003651439656	21.265409793803542
50	19.954441528364164	19.096099636969136	38.68877816236228	22.260680672304424
51	18.76645577912171	19.973036822909403	36.56595624189097	24.694551156077914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	259.5
2	453.0
3	10524.5
4	20596.0
5	14109.5
6	7623.0
7	7277.0
8	6931.0
9	6605.5
10	6280.0
11	5825.0
12	5370.0
13	4905.5
14	4441.0
15	4096.0
16	3751.0
17	3389.5
18	3028.0
19	2706.5
20	2385.0
21	2185.5
22	1986.0
23	1879.5
24	1773.0
25	1769.5
26	1816.0
27	1866.0
28	2195.5
29	2525.0
30	2559.0
31	2593.0
32	3084.5
33	3576.0
34	4095.5
35	4615.0
36	4782.0
37	4949.0
38	5342.5
39	5736.0
40	6782.0
41	7828.0
42	8238.0
43	8648.0
44	9874.0
45	11100.0
46	21113.5
47	31127.0
48	24799.5
49	18472.0
50	16818.5
51	15165.0
52	12786.5
53	10408.0
54	9232.5
55	8057.0
56	7714.0
57	7371.0
58	6972.0
59	6573.0
60	6278.0
61	5983.0
62	5215.0
63	4447.0
64	3853.0
65	3259.0
66	2600.0
67	1941.0
68	1656.5
69	1372.0
70	1207.0
71	1042.0
72	847.0
73	652.0
74	514.5
75	312.5
76	248.0
77	183.0
78	118.0
79	96.5
80	75.0
81	50.0
82	25.0
83	20.0
84	15.0
85	10.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	236619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.324987616639696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07310280476536	34.691661874423765
2	6.917255759269018	5.993800402229196
3	2.9858608467778285	3.880871526349762
4	2.0275669484619856	3.5137725173609384
5	1.5066640839891405	3.2638101390632652
6	1.2387231708509403	3.2200599622536985
7	0.9597854793599779	2.9107885805531146
8	0.7858243599248385	2.723666453007795
9	0.6618520689846223	2.5807259423525513
>10	2.74138727061318	17.630195922299073
>50	0.053987933119293424	1.5177683286259491
>100	0.03699173195210846	2.9125211876943156
>500	0.004998882696230873	1.6425164104308214
>1k	0.003999106156984698	4.7308977412250615
>5k	9.997765392461746E-4	2.944141361207356
>10k+	9.997765392461746E-4	5.842801650923352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13489	5.700725639107595	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	6797	2.872550386908913	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	4960	2.096196839645168	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	3272	1.382813721636893	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT	1399	0.591245842472498	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1291	0.5456028467705467	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC	912	0.3854297414831438	No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	899	0.37993567718568666	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA	842	0.3558463183429902	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	589	0.2489233747078637	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTACTAGGGTCGTATGCCG	550	0.23244118181549242	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGT	328	0.1386194684281482	No Hit
GAATCTTTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	319	0.13481588545298562	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	304	0.12847658049438127	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGCCTCCTATACACATCTGACGCTACTAGGGTCGTATGC	276	0.11664321123831982	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	261	0.1103039062797155	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT	247	0.10438722165168478	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	238	0.10058363867652217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1610183459485502	0.0
2	0.0	0.0	0.0	1.0793723242850322	0.0
3	0.0	0.0	0.0	1.2737776763488984	0.0
4	0.0	0.0	0.0	1.9825119707208636	0.0
5	0.0	0.0	0.0	3.911351159458877	0.0
6	0.0	0.0	0.0	4.232119990364256	0.0
7	0.0	0.0	0.0	4.691085669367211	0.0
8	0.0	0.0	0.0	5.364742476301565	0.0
9	0.0	0.0	0.0	5.535481089853309	0.0
10	0.0	0.0	0.0	9.359349840883446	0.0
11	0.0	0.0	0.0	9.959893330628564	0.0
12	0.0	0.0	0.0	12.912741580346465	0.0
13	4.226203305736226E-4	0.0	0.0	13.200546025467101	0.0
14	4.226203305736226E-4	0.0	0.0	13.382695387944333	0.0
15	4.226203305736226E-4	0.0	0.0	13.985774599672892	0.0
16	4.226203305736226E-4	0.0	0.0	14.33781733504072	0.0
17	4.226203305736226E-4	0.0	0.0	14.640836112062006	0.0
18	4.226203305736226E-4	0.0	0.0	14.926104835199203	0.0
19	4.226203305736226E-4	0.0	0.0	15.940393628575896	0.0
20	4.226203305736226E-4	0.0	0.0	16.25355529353095	0.0
21	4.226203305736226E-4	0.0	0.0	16.572633643114035	0.0
22	4.226203305736226E-4	0.0	0.0	17.04681365401764	0.0
23	4.226203305736226E-4	0.0	0.0	17.3422252650886	0.0
24	4.226203305736226E-4	0.0	0.0	17.60213676839138	0.0
25	4.226203305736226E-4	0.0	0.0	17.82020885896737	0.0
26	4.226203305736226E-4	0.0	0.0	18.022643997312134	0.0
27	4.226203305736226E-4	0.0	0.0	18.222120793342885	0.0
28	4.226203305736226E-4	0.0	0.0	18.396240369539218	0.0
29	4.226203305736226E-4	0.0	0.0	18.586419518297348	0.0
30	4.226203305736226E-4	0.0	0.0	18.800688025898175	0.0
31	4.226203305736226E-4	0.0	0.0	19.007349367548674	0.0
32	4.226203305736226E-4	0.0	0.0	19.218236912504914	0.0
33	4.226203305736226E-4	0.0	0.0	19.433350660766887	0.0
34	4.226203305736226E-4	0.0	0.0	19.65860729696263	0.0
35	4.226203305736226E-4	0.0	0.0	19.868226980927144	0.0
36	4.226203305736226E-4	0.0	0.0	20.07319784125535	0.0
37	4.226203305736226E-4	0.0	0.0	20.281127043897573	0.0
38	4.226203305736226E-4	0.0	0.0	20.497931273481843	0.0
39	4.226203305736226E-4	0.0	0.0	20.71600336405783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.881387E-5	45.0	6
ATGATCC	70	0.0	45.0	3
TCGGGAT	35	1.2064629E-7	45.0	5
CGACCCT	25	3.881387E-5	45.0	12
TACGGAT	25	3.881387E-5	45.0	29
CCCGTCT	35	1.2064629E-7	45.0	18
CGGATTT	20	7.0219213E-4	45.0	33
CGGGATG	50	2.1827873E-11	45.0	6
TAGGGCG	20	7.0219213E-4	45.0	5
TCGAGGG	20	7.0219213E-4	45.0	2
ATACGGA	25	3.881387E-5	45.0	28
CCTATGC	20	7.0219213E-4	45.0	35
AGGGCGA	25	3.881387E-5	45.0	6
CCTTGCG	20	7.0219213E-4	45.0	1
CCGTGTC	30	2.1581727E-6	44.999996	19
TTATACG	30	2.1581727E-6	44.999996	26
CTACGGC	60	0.0	44.999996	6
TAGGGAC	30	2.1581727E-6	44.999996	5
TTACGGG	30	2.1581727E-6	44.999996	3
TATACGG	30	2.1581727E-6	44.999996	27
>>END_MODULE