##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933436.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 601186 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.392836825874188 31.0 28.0 33.0 25.0 34.0 2 28.52900932490111 31.0 27.0 33.0 16.0 34.0 3 28.47170759132781 31.0 26.0 33.0 16.0 34.0 4 33.12632529699627 35.0 32.0 37.0 28.0 37.0 5 34.27215703625833 35.0 33.0 37.0 30.0 37.0 6 33.647476820817516 35.0 33.0 37.0 28.0 37.0 7 33.812362563333146 35.0 33.0 37.0 29.0 37.0 8 33.537406060686706 35.0 33.0 37.0 28.0 37.0 9 34.93409859843709 37.0 34.0 39.0 28.0 39.0 10 34.8880097008247 37.0 34.0 39.0 28.0 39.0 11 35.2414743523635 37.0 34.0 39.0 30.0 39.0 12 35.37671868606388 37.0 34.0 39.0 30.0 39.0 13 35.45150918351392 37.0 34.0 39.0 30.0 39.0 14 36.31330902582562 38.0 35.0 40.0 30.0 41.0 15 36.51611148629542 38.0 34.0 41.0 30.0 41.0 16 36.50731054948053 38.0 34.0 41.0 30.0 41.0 17 35.704560651778316 38.0 33.0 40.0 27.0 41.0 18 35.43517480446983 38.0 34.0 39.0 27.0 40.0 19 34.6899578499832 37.0 33.0 37.0 27.0 40.0 20 33.64122584358252 35.0 32.0 37.0 27.0 39.0 21 34.106103601880285 35.0 33.0 38.0 27.0 40.0 22 34.50770809699494 35.0 33.0 38.0 28.0 40.0 23 34.800537604002756 35.0 33.0 39.0 30.0 40.0 24 34.2505247959866 35.0 33.0 38.0 27.0 40.0 25 33.34637200467077 35.0 33.0 38.0 24.0 40.0 26 33.20381046797497 35.0 32.0 38.0 24.0 40.0 27 34.01341348600932 35.0 33.0 38.0 25.0 40.0 28 33.676577631548305 35.0 33.0 38.0 24.0 40.0 29 33.674445180027476 35.0 33.0 39.0 23.0 40.0 30 32.91828984706896 35.0 32.0 38.0 21.0 40.0 31 32.786054565475574 35.0 32.0 38.0 21.0 40.0 32 31.95921395375142 35.0 31.0 38.0 15.0 40.0 33 30.944389922586353 35.0 27.0 39.0 10.0 40.0 34 30.139575771890897 35.0 23.0 39.0 7.0 40.0 35 29.486034272255175 35.0 21.0 39.0 7.0 40.0 36 28.732608543778465 35.0 18.0 39.0 7.0 40.0 37 28.66054598743151 35.0 18.0 39.0 7.0 40.0 38 28.392392703755576 35.0 17.0 39.0 7.0 40.0 39 28.057970411819305 35.0 15.0 39.0 7.0 40.0 40 27.785826682590745 34.0 15.0 38.0 7.0 40.0 41 27.336807577022753 33.0 15.0 38.0 7.0 40.0 42 27.4943478391047 34.0 15.0 38.0 7.0 40.0 43 27.369112055170945 34.0 15.0 38.0 7.0 40.0 44 27.493048740323296 34.0 15.0 39.0 7.0 40.0 45 27.58835867768045 34.0 15.0 39.0 7.0 40.0 46 27.37610822607313 34.0 13.0 38.0 7.0 40.0 47 27.253859870322994 34.0 12.0 38.0 7.0 40.0 48 27.105651162868064 34.0 12.0 38.0 7.0 40.0 49 27.170750815887263 34.0 12.0 38.0 7.0 40.0 50 27.125184219193393 34.0 12.0 38.0 7.0 40.0 51 26.001686666023495 31.0 10.0 37.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 11.0 9 34.0 10 41.0 11 43.0 12 53.0 13 70.0 14 120.0 15 189.0 16 337.0 17 728.0 18 1585.0 19 2816.0 20 4496.0 21 6938.0 22 9808.0 23 14237.0 24 20653.0 25 30069.0 26 37930.0 27 37833.0 28 32339.0 29 28394.0 30 27468.0 31 29519.0 32 32754.0 33 37816.0 34 42940.0 35 50234.0 36 53955.0 37 53004.0 38 39138.0 39 5634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.506106263286238 2.183849923318241 27.14301397570802 44.16702983768751 2 43.87227912825648 17.71082493604309 27.005119879704452 11.41177605599598 3 9.755549863103932 17.902446164747683 60.27968049821519 12.062323473933192 4 9.005199721883079 2.3786315715934836 75.55249124231104 13.063677464212406 5 20.625397131669732 2.7347609558439485 61.55732169411796 15.082520218368359 6 11.433898992990523 14.798914146370674 64.98171946785189 8.785467392786925 7 43.54076774908265 1.0100035596304637 53.04714347972175 2.4020852115651397 8 42.14968412438081 11.509416386941812 43.094982251749045 3.2459172369283382 9 39.59905919299518 2.2473909904754934 41.93411024208814 16.21943957444119 10 25.986466750722737 13.303536675837426 48.620726364220054 12.089270209219775 11 21.52528501994391 13.409992914006647 52.61167093046078 12.453051135588652 12 15.87561919272904 11.64398372550259 55.562172106469546 16.918224975298827 13 15.339512230823738 12.748799872252514 61.6014012302349 10.310286666688844 14 13.403838412737489 18.903800155026897 53.21015459441837 14.48220683781725 15 9.952992917333404 13.73584880552774 62.83745795810282 13.47370031903604 16 11.606724042143362 15.359472775480468 52.40840605070643 20.625397131669732 17 12.130854677254627 16.250378418659118 57.294248369057165 14.324518535029092 18 12.179924349535751 15.067383472003673 55.66180849188105 17.090883686579527 19 12.328464069356238 17.345214293080677 52.69517254227477 17.63114909528831 20 14.429477732349058 16.275661775224307 58.246200011310975 11.048660481115661 21 14.380408060067932 20.781089379992217 53.45899605113892 11.379506508800937 22 12.544370627393187 12.874052289973486 56.62124533838113 17.960331744252194 23 13.332645803461823 19.68791688429205 54.76940580785315 12.210031504392983 24 14.906202073900591 15.656385877249305 52.266187170027244 17.171224878822862 25 11.755097424091712 23.81592385717565 50.74985112760444 13.679127591128204 26 12.382690215673685 14.114600140389163 55.96770383874541 17.535005805191737 27 15.508844184661651 16.768188214629085 51.75968834936276 15.963279251346504 28 11.302492074000392 16.57640064805235 56.7190520071991 15.402055270748155 29 14.94479246023693 13.556037565745044 55.386352975618195 16.11281699839983 30 12.815667696852554 18.58659383285705 56.54356555209203 12.054172918198361 31 18.035350124587065 14.767642626408467 49.73169701223914 17.465310236765326 32 16.64343481052453 18.84125711510248 48.14566540138992 16.36964267298307 33 14.95693512490311 16.090860399277428 48.55452389110857 20.39768058471089 34 16.653082407108617 18.230464448606586 49.739847567973975 15.376605576310826 35 14.592322509173533 19.34526086768488 44.60749252311265 21.454924100028943 36 22.192798900839342 19.245458144401233 42.04938238748074 16.51236056727868 37 15.287614814716244 20.969051175509744 45.10517543655374 18.638158573220267 38 20.756138699171306 20.82483624036488 40.73647756268443 17.68254749777939 39 18.93873110817617 20.730189991117555 41.4653701183993 18.86570878230697 40 16.917725961682407 21.021114929489375 43.060550312216186 19.00060879661203 41 20.6353774039981 23.30709630630121 39.61652466956982 16.441001620130873 42 16.162219346425232 22.13790740303334 41.481172216252546 20.218701034288888 43 21.45808451959959 20.833818485460405 38.02766531489423 19.680431680045775 44 19.379859145089874 21.517966153569777 38.90443223894103 20.197742462399322 45 17.098202552953662 21.150026780397415 37.80527157984384 23.94649908680508 46 21.576517084562845 23.3771245504719 35.819197386499354 19.2271609784659 47 15.23837880456298 20.546053966659237 45.86201275478803 18.353554473989746 48 18.407115268818636 23.513521605626213 36.38358178666835 21.6957813388868 49 15.646904618537357 21.018786199279425 41.89186042256473 21.442448759618486 50 18.53186867292319 20.197742462399322 39.390804177076646 21.879584687600843 51 17.305958555255778 20.85244832714002 37.44498374878989 24.39660936881431 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61.0 1 1038.0 2 2015.0 3 24306.0 4 46597.0 5 35297.0 6 23997.0 7 22777.5 8 21558.0 9 21241.5 10 20925.0 11 20003.0 12 19081.0 13 18155.0 14 17229.0 15 16240.0 16 15251.0 17 14275.0 18 13299.0 19 12363.0 20 11427.0 21 10566.5 22 9706.0 23 9200.0 24 8694.0 25 8517.5 26 8153.5 27 7966.0 28 8271.5 29 8577.0 30 8754.0 31 8931.0 32 9521.0 33 10111.0 34 10780.0 35 11449.0 36 12124.0 37 12799.0 38 13207.5 39 13616.0 40 14042.0 41 14468.0 42 15599.5 43 16731.0 44 18734.0 45 20737.0 46 56116.0 47 91495.0 48 62186.5 49 32878.0 50 29838.5 51 26799.0 52 22862.0 53 18925.0 54 18169.0 55 17413.0 56 16528.0 57 15643.0 58 14752.0 59 13861.0 60 12501.5 61 11142.0 62 9858.0 63 8574.0 64 7624.0 65 6674.0 66 5743.5 67 4813.0 68 4075.5 69 3338.0 70 2813.5 71 2289.0 72 1936.5 73 1584.0 74 1309.0 75 801.5 76 569.0 77 435.0 78 301.0 79 227.5 80 154.0 81 111.5 82 69.0 83 51.5 84 34.0 85 27.5 86 21.0 87 11.0 88 1.0 89 2.0 90 3.0 91 2.0 92 1.0 93 2.0 94 3.0 95 2.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 601186.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.072759260404986 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68849086061084 41.90513655729969 2 6.498726890766966 6.508183742009891 3 2.894737735035003 4.34842517185253 4 1.8564712938419943 3.718345606816109 5 1.2860027973407944 3.2196854239726487 6 0.9437618747714628 2.835405669274796 7 0.6983300126545865 2.4477117425578068 8 0.5127405249282873 2.0539466294230246 9 0.3644978203732726 1.6426270449443896 >10 1.1644080656368274 8.860601288779264 >50 0.052668424272261004 1.8913839860838229 >100 0.032073719637370234 3.0855863690063847 >500 0.003038562913014022 0.9483968622685632 >1k 0.002700944811568019 3.8412608742364163 >5k 0.0 0.0 >10k+ 0.0013504724057840096 12.693303031474654 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24817 4.1280069728836 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG 17494 2.9099147352067414 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC 17236 2.8669995641947748 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC 15537 2.584391519429927 No Hit GAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT 4238 0.704939902126796 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGCTTCGT 3482 0.5791884707894063 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTC 3368 0.5602259533655142 No Hit GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC 3182 0.5292871091475849 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT 2885 0.4798847611221818 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTA 2678 0.44545282158932514 No Hit GAATGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT 1732 0.2880971945454485 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGT 1157 0.19245291806529094 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTAT 774 0.12874551303589904 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.12541875559311094 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 732 0.12175932240604405 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 647 0.10762060327419466 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06470543226222833 0.0 2 0.0 0.0 0.0 0.6646861370690602 0.0 3 0.0 0.0 0.0 0.7724730782153942 0.0 4 0.0 0.0 0.0 1.6151407384736172 0.0 5 0.0 0.0 0.0 4.98547870376223 0.0 6 0.0 0.0 0.0 5.400658032622183 0.0 7 0.0 0.0 0.0 6.021098295702162 0.0 8 0.0 0.0 0.0 6.735852132285183 0.0 9 0.0 0.0 0.0 6.869587781485264 0.0 10 0.0 0.0 0.0 10.317106519446561 0.0 11 0.0 0.0 0.0 10.505400990708367 0.0 12 0.0 0.0 0.0 13.595625979314223 0.0 13 0.0 0.0 0.0 13.805045360337733 0.0 14 0.0 0.0 0.0 13.99300715585526 0.0 15 0.0 0.0 0.0 14.567371828352623 0.0 16 0.0 0.0 0.0 14.862122537783648 0.0 17 0.0 0.0 0.0 15.003343391230002 0.0 18 0.0 0.0 0.0 15.10996596727136 0.0 19 0.0 0.0 0.0 15.900736211422089 0.0 20 0.0 0.0 0.0 16.0536007159182 0.0 21 0.0 0.0 0.0 16.15273808771329 0.0 22 0.0 0.0 0.0 16.40324292315523 0.0 23 0.0 0.0 0.0 16.523671542584157 0.0 24 0.0 0.0 0.0 16.63578326840612 0.0 25 0.0 0.0 0.0 16.716789812138007 0.0 26 0.0 0.0 0.0 16.851523488570926 0.0 27 0.0 0.0 0.0 17.002724614345645 0.0 28 0.0 0.0 0.0 17.103525364862122 0.0 29 0.0 0.0 0.0 17.208817237926365 0.0 30 0.0 0.0 0.0 17.3275824786339 0.0 31 0.0 0.0 0.0 17.441191245305113 0.0 32 0.0 0.0 0.0 17.57093478557385 0.0 33 0.0 0.0 0.0 17.68304651139581 0.0 34 0.0 0.0 0.0 17.875832105205376 0.0 35 0.0 0.0 0.0 18.00923507866118 0.0 36 0.0 0.0 0.0 18.150123256363255 0.0 37 0.0 0.0 0.0 18.29084509619319 0.0 38 0.0 0.0 0.0 18.45019677770274 0.0 39 0.0 0.0 0.0 18.661445875319785 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCA 70 0.0 45.0 16 TTATCGG 35 1.2106284E-7 45.0 2 ACGGGCT 20 7.0305984E-4 45.0 5 ATACGGA 20 7.0305984E-4 45.0 4 TATACCG 20 7.0305984E-4 45.0 27 TGCGTTG 25 3.8885788E-5 45.0 1 CGTTTTT 19795 0.0 44.329376 1 CGATGAA 650 0.0 44.307693 19 GATACCT 2040 0.0 43.566177 5 CCGATGA 670 0.0 42.985073 18 TGATACC 2075 0.0 42.831326 4 ATACCTG 2100 0.0 42.321426 6 ACGGCTG 1965 0.0 41.564884 8 GATGAAT 710 0.0 41.514084 20 TACGGCT 1975 0.0 41.468353 7 TACCTGT 2175 0.0 41.06897 7 CGGCTGT 2005 0.0 40.960102 9 ATGAATG 660 0.0 40.90909 21 GAATCTG 1985 0.0 40.80605 1 TTGATAC 105 0.0 40.714287 3 >>END_MODULE