##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833845
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.3242233268773	31.0	28.0	31.0	25.0	34.0
2	28.483128159310183	31.0	27.0	33.0	16.0	34.0
3	28.48773453099797	31.0	26.0	33.0	16.0	34.0
4	33.02804957755938	35.0	32.0	37.0	28.0	37.0
5	34.19426392195192	35.0	33.0	37.0	30.0	37.0
6	33.49781074420306	35.0	33.0	37.0	28.0	37.0
7	33.562364708069246	35.0	32.0	37.0	29.0	37.0
8	33.41864255347217	35.0	33.0	37.0	28.0	37.0
9	34.66341466339667	37.0	34.0	39.0	28.0	39.0
10	34.677810624276695	37.0	33.0	39.0	27.0	39.0
11	34.85514454125167	37.0	34.0	39.0	27.0	39.0
12	35.002251017875025	37.0	34.0	39.0	27.0	39.0
13	35.05328568259089	37.0	34.0	39.0	27.0	39.0
14	35.83119404685523	38.0	34.0	40.0	27.0	41.0
15	36.10825872914031	38.0	34.0	40.0	27.0	41.0
16	36.080165978089454	38.0	34.0	40.0	29.0	41.0
17	35.33344086730747	37.0	33.0	40.0	27.0	41.0
18	35.144111915284014	37.0	33.0	39.0	27.0	40.0
19	34.405972332987545	37.0	33.0	38.0	27.0	40.0
20	33.72155736377864	35.0	32.0	38.0	27.0	40.0
21	34.15614292824206	35.0	33.0	38.0	27.0	40.0
22	34.67722778214176	35.0	33.0	39.0	28.0	40.0
23	34.98746049925346	35.0	33.0	39.0	29.0	40.0
24	34.49486535267346	35.0	33.0	39.0	27.0	40.0
25	33.5401975187235	35.0	33.0	38.0	24.0	40.0
26	33.52329389754691	35.0	32.0	38.0	24.0	40.0
27	34.224189147863214	35.0	33.0	38.0	26.0	40.0
28	33.90799009408224	35.0	33.0	39.0	24.0	40.0
29	33.906632527628034	35.0	33.0	39.0	24.0	40.0
30	33.23264875366525	35.0	32.0	39.0	21.0	40.0
31	33.192420653718614	35.0	33.0	39.0	21.0	40.0
32	32.544598816326776	35.0	31.0	39.0	18.0	40.0
33	31.792980709844155	35.0	30.0	39.0	13.0	40.0
34	31.366594510970263	35.0	30.0	39.0	10.0	40.0
35	30.95511276076489	35.0	27.0	39.0	8.0	41.0
36	30.358792101649588	35.0	24.0	39.0	7.0	40.0
37	30.321414651404037	35.0	23.0	39.0	7.0	40.0
38	29.83585918246197	35.0	23.0	39.0	7.0	40.0
39	29.596496950872165	35.0	22.0	39.0	7.0	40.0
40	29.373118505237784	35.0	21.0	39.0	7.0	40.0
41	28.862565584730977	34.0	20.0	39.0	7.0	40.0
42	29.199760147269576	35.0	21.0	39.0	7.0	40.0
43	29.11529001193267	35.0	20.0	39.0	7.0	40.0
44	29.27746403708123	35.0	20.0	39.0	7.0	40.0
45	29.318728300823295	35.0	20.0	39.0	7.0	40.0
46	29.133753875120675	35.0	20.0	39.0	7.0	40.0
47	29.074264401657384	35.0	20.0	39.0	7.0	40.0
48	28.928598240680223	35.0	20.0	38.0	7.0	40.0
49	29.133352121797216	35.0	20.0	39.0	7.0	40.0
50	29.11570855494726	35.0	20.0	39.0	7.0	40.0
51	28.181400620019307	33.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	46.0
10	61.0
11	66.0
12	77.0
13	105.0
14	159.0
15	253.0
16	452.0
17	919.0
18	1887.0
19	3253.0
20	5384.0
21	7849.0
22	10896.0
23	15611.0
24	22402.0
25	31746.0
26	41481.0
27	45367.0
28	42506.0
29	39663.0
30	40104.0
31	43450.0
32	48954.0
33	57110.0
34	64426.0
35	75142.0
36	80099.0
37	82045.0
38	62985.0
39	9327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.313295636479204	2.459689750493197	29.494330481084614	39.73268413194299
2	38.33386300811302	17.58936013287841	30.74516247024327	13.331614388765297
3	12.387554041818323	17.806306927546487	56.70022606119842	13.105912969436767
4	10.817118289370327	2.896581498959639	70.56095557327801	15.725344638392027
5	22.153637666472786	3.536988289190437	57.491140439769985	16.818233604566796
6	13.327177113252464	15.757364977903569	60.299216281203336	10.616241627640628
7	50.463455438360846	1.2697803548621147	45.66867943082947	2.598084775947568
8	49.12004029525871	12.050321102842855	35.40646043329396	3.4231781686044767
9	45.965856963824216	3.1207238755404183	34.55162530206453	16.361793858570834
10	27.97522321294725	19.467886717555423	40.43833086484898	12.118559204648346
11	24.900431135282936	14.671431740911082	46.59834861395103	13.829788509854948
12	18.62696304469056	13.648819624750402	49.37620301135103	18.348014319208005
13	18.17711924878125	14.324364839988245	55.64715264827396	11.851363262956545
14	15.212659427111753	20.86946614778526	47.695674855638636	16.222199569464347
15	11.734794835970714	14.761136662089477	57.44352967278091	16.060538829158897
16	14.236938519748874	16.89138868734597	46.91303539626669	21.958637396638466
17	14.535914948221793	17.43309607900749	52.16605004527221	15.864938927498514
18	15.009624090808243	15.812171326805341	50.569830124303685	18.60837445808274
19	14.406154621062667	18.00622417835449	47.57826694409633	20.009354256486517
20	17.012034610748998	17.55626045607997	52.75045122294911	12.681253710221924
21	16.546600387362158	22.549514598036804	47.77470633031319	13.129178684287849
22	14.645407719660128	13.97022228351792	52.06759049943335	19.316779497388602
23	15.300925231907609	21.37519562988325	49.8144139498348	13.509465188374339
24	16.328934034502815	17.034340914678385	47.68907890555199	18.947646145266805
25	13.66429012586272	25.245459287997168	45.19724888918204	15.893001696958068
26	14.526680618100487	15.500362777254765	49.992504602174265	19.980452002470482
27	17.240734189207828	17.9624510550522	45.9483477145033	18.84846704123668
28	12.594906727269455	18.605616151682867	50.53589096294875	18.263586158098928
29	16.873879438025053	16.313943238851344	46.01658581630878	20.795591506814816
30	14.83153343846878	20.416504266380443	47.96478961917383	16.78717267597695
31	20.471310615282217	17.20355701599218	43.05356511102183	19.27156725770377
32	18.833116466489574	23.587956994405435	41.97878502599404	15.600141513110948
33	16.437107615923825	20.84296242107346	40.2192253956071	22.50070456739562
34	18.473457297219507	22.8665999076567	39.560829650594535	19.09911314452926
35	15.493646900802908	22.178342497706407	38.229527070378786	24.098483531111896
36	20.281467179152003	22.2425031030947	37.47878802415317	19.997241693600127
37	16.572744334978324	25.682830741924455	37.63960927990214	20.104815643195078
38	21.57463317523041	25.880229539063016	33.77786039371826	18.76727689198832
39	19.83030419322536	27.799890867007658	34.36214164502995	18.007663294737032
40	18.60513644622202	24.611288668757382	35.723665669279065	21.059909215741534
41	18.581630878640514	26.895166367850138	35.91722682273084	18.605975930778502
42	18.25087396338648	22.58609213942639	38.17819858606815	20.984835311118974
43	20.016309985668798	21.812447157445327	36.462771858079144	21.708470998806735
44	21.15165288512853	22.300787316587613	35.04560200037177	21.501957797912084
45	19.001972788707732	21.151892737858955	35.29456913455139	24.551565338881925
46	22.29718952563126	24.339775377917956	34.04889397909683	19.314141117353945
47	15.699800322601924	22.864920938543733	42.71885062571581	18.716428113138534
48	19.39077406472426	25.154555103166654	33.777500614622625	21.677170217486463
49	17.638529942615232	21.121071661999533	39.92912351815985	21.311274877225383
50	18.451270919655332	20.816338767996452	38.29560649761047	22.43678381473775
51	18.321990297957054	21.147935167806967	35.194430619599565	25.335643914636414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	1248.0
2	2415.0
3	30131.0
4	57847.0
5	43065.5
6	28284.0
7	26758.0
8	25232.0
9	24000.0
10	22768.0
11	21664.0
12	20560.0
13	19215.5
14	17871.0
15	16185.0
16	14499.0
17	13413.0
18	12327.0
19	11411.0
20	10495.0
21	9680.5
22	8866.0
23	8513.0
24	8160.0
25	8076.5
26	8588.0
27	9183.0
28	9924.5
29	10666.0
30	11415.0
31	12164.0
32	13104.5
33	14045.0
34	15761.5
35	17478.0
36	18290.5
37	19103.0
38	19991.5
39	20880.0
40	22136.5
41	23393.0
42	24770.5
43	26148.0
44	28697.0
45	31246.0
46	77434.5
47	123623.0
48	87719.0
49	51815.0
50	47933.0
51	44051.0
52	39835.0
53	35619.0
54	34280.0
55	32941.0
56	31244.5
57	29548.0
58	27398.0
59	25248.0
60	22913.0
61	20578.0
62	18102.5
63	15627.0
64	13531.5
65	11436.0
66	9504.5
67	7573.0
68	6276.5
69	4980.0
70	4120.0
71	3260.0
72	2750.0
73	2240.0
74	1968.5
75	1401.0
76	1105.0
77	765.5
78	426.0
79	301.0
80	176.0
81	145.5
82	115.0
83	80.0
84	45.0
85	31.0
86	17.0
87	16.5
88	16.0
89	9.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.533585505225886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.21342333007857	33.729384097322985
2	5.495667060619411	4.455181814197406
3	2.146434304662813	2.6100803525820058
4	1.3991225176393827	2.2684580880409135
5	1.02854972411033	2.0845404094301276
6	0.837536464113899	2.0369013529143167
7	0.7394525367440259	2.098086383761911
8	0.6530346958981648	2.117587014725396
9	0.5720696808324431	2.0869231790672207
>10	3.7741672889258635	24.510934502342806
>50	0.07871581737453345	2.2268425329756885
>100	0.0548899384298504	4.098068890357298
>500	0.003317523751253888	0.9409311890068238
>1k	0.002412744546366464	3.5761986492406943
>5k	0.0	0.0
>10k+	0.001206372273183232	11.159881544034423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26485	3.1762497826334632	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCG	23587	2.8287031762497827	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	20929	2.509938897516925	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGC	20289	2.4331860237813983	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	4902	0.5878790422680474	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTT	4828	0.5790044912423772	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTC	4573	0.5484232681133784	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTA	4423	0.5304343133316144	No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	3888	0.46627370794332285	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	3785	0.4539212923265115	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGT	1563	0.18744490882598086	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	1292	0.15494486385359388	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTAT	984	0.11800754336837181	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATG	964	0.11560901606413661	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1992636521175998E-4	0.0	0.0	0.06871780726633847	0.0
2	1.1992636521175998E-4	0.0	0.0	0.6336909137789397	0.0
3	2.3985273042351996E-4	0.0	0.0	0.7227962031312773	0.0
4	2.3985273042351996E-4	0.0	0.0	1.4526680618100487	0.0
5	2.3985273042351996E-4	0.0	0.0	4.4029765723845555	0.0
6	2.3985273042351996E-4	0.0	0.0	4.642589450077653	0.0
7	2.3985273042351996E-4	0.0	0.0	5.241261865214758	0.0
8	2.3985273042351996E-4	0.0	0.0	5.956982412798542	0.0
9	2.3985273042351996E-4	0.0	0.0	6.051844167681044	0.0
10	2.3985273042351996E-4	0.0	0.0	9.46746697527718	0.0
11	2.3985273042351996E-4	0.0	0.0	9.627688599200091	0.0
12	2.3985273042351996E-4	0.0	0.0	12.709796185142322	0.0
13	2.3985273042351996E-4	0.0	0.0	12.94545149278343	0.0
14	2.3985273042351996E-4	0.0	0.0	13.133016327974623	0.0
15	3.597790956352799E-4	0.0	0.0	13.835425049019902	0.0
16	3.597790956352799E-4	0.0	0.0	14.139798163927349	0.0
17	3.597790956352799E-4	0.0	0.0	14.27615444117312	0.0
18	3.597790956352799E-4	0.0	0.0	14.37977082071608	0.0
19	3.597790956352799E-4	0.0	0.0	15.211220310729212	0.0
20	3.597790956352799E-4	0.0	0.0	15.388711331242616	0.0
21	3.597790956352799E-4	0.0	0.0	15.506958727341413	0.0
22	3.597790956352799E-4	0.0	0.0	15.74189447679125	0.0
23	3.597790956352799E-4	0.0	0.0	15.844671371777729	0.0
24	3.597790956352799E-4	0.0	0.0	15.956202891424665	0.0
25	4.797054608470399E-4	0.0	0.0	16.040511126168532	0.0
26	4.797054608470399E-4	0.0	0.0	16.157799111345632	0.0
27	4.797054608470399E-4	0.0	0.0	16.324017053529133	0.0
28	4.797054608470399E-4	0.0	0.0	16.41636035474219	0.0
29	4.797054608470399E-4	0.0	0.0	16.519856807919936	0.0
30	4.797054608470399E-4	0.0	0.0	16.64817801869652	0.0
31	4.797054608470399E-4	0.0	0.0	16.761148654725996	0.0
32	4.797054608470399E-4	0.0	0.0	16.887790896389618	0.0
33	4.797054608470399E-4	0.0	0.0	17.003519838818963	0.0
34	4.797054608470399E-4	0.0	0.0	17.16062337724637	0.0
35	4.797054608470399E-4	0.0	0.0	17.311490744682764	0.0
36	4.797054608470399E-4	0.0	0.0	17.431417109894525	0.0
37	4.797054608470399E-4	0.0	0.0	17.567173755314236	0.0
38	4.797054608470399E-4	0.0	0.0	17.72271825099389	0.0
39	4.797054608470399E-4	0.0	0.0	17.97540310249507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTG	20	7.032171E-4	45.0	1
CGTTTTT	22440	0.0	44.31818	1
GATACCT	3085	0.0	42.447327	5
CCGATGA	760	0.0	42.33553	18
CGGCTGT	2495	0.0	42.29459	9
TGATACC	3115	0.0	42.182983	4
CGATGAA	780	0.0	42.115383	19
TACGGCT	2540	0.0	41.633858	7
GGCGACT	595	0.0	41.596638	10
ATACCTG	3170	0.0	41.451103	6
ACCGGTC	60	3.6379788E-12	41.249996	17
ACGGCTG	2565	0.0	41.22807	8
CCGGTGA	105	0.0	40.714287	18
GATACGC	50	1.0804797E-9	40.5	5
GATGAAT	830	0.0	40.391567	20
GAATCTG	2455	0.0	40.23422	1
ATGATAC	9515	0.0	40.128746	3
TACCTGT	3290	0.0	40.07599	7
TACGGGA	90	0.0	40.0	4
CACAACG	45	1.9272193E-8	40.0	12
>>END_MODULE