##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933433.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	588147
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.470810868711393	31.0	28.0	31.0	16.0	33.0
2	28.744320722540454	31.0	30.0	33.0	16.0	34.0
3	28.938421857120755	31.0	30.0	33.0	16.0	34.0
4	31.964950939135964	35.0	30.0	37.0	19.0	37.0
5	33.59384473609489	35.0	33.0	37.0	28.0	37.0
6	32.61749018527681	35.0	33.0	37.0	17.0	37.0
7	32.09596240395683	35.0	31.0	35.0	25.0	37.0
8	32.828639778830805	35.0	32.0	36.0	27.0	37.0
9	32.936211525349954	35.0	31.0	37.0	25.0	39.0
10	31.94055567740718	35.0	28.0	39.0	15.0	39.0
11	32.44550597044616	35.0	29.0	39.0	17.0	39.0
12	33.34760697580707	35.0	31.0	39.0	23.0	39.0
13	33.38229898307736	35.0	32.0	39.0	24.0	39.0
14	33.599822833407295	36.0	31.0	40.0	20.0	41.0
15	34.18209563255444	37.0	32.0	40.0	25.0	41.0
16	34.37652831690037	37.0	32.0	40.0	25.0	41.0
17	32.376904073301404	36.0	27.0	39.0	18.0	41.0
18	33.08401641086327	36.0	30.0	39.0	23.0	39.0
19	32.748613866941426	36.0	30.0	37.0	20.0	39.0
20	32.32035188481791	34.0	30.0	36.0	24.0	39.0
21	32.6420503717608	35.0	31.0	37.0	23.0	39.0
22	32.74854925724351	34.0	30.0	37.0	24.0	39.0
23	33.79426571928447	35.0	32.0	38.0	27.0	40.0
24	32.06899125558746	34.0	30.0	37.0	21.0	39.0
25	31.3103203790889	34.0	29.0	37.0	18.0	39.0
26	30.373370942978543	33.0	25.0	37.0	18.0	39.0
27	32.16443338145056	34.0	30.0	37.0	22.0	39.0
28	32.17223925311189	35.0	31.0	37.0	21.0	39.0
29	32.280861757349776	35.0	30.0	38.0	21.0	40.0
30	31.328514810072992	34.0	29.0	37.0	18.0	40.0
31	31.70195716377028	35.0	30.0	37.0	20.0	40.0
32	31.42503659799336	35.0	30.0	37.0	17.0	40.0
33	31.145965209377927	35.0	30.0	38.0	15.0	40.0
34	30.299352032740114	35.0	27.0	38.0	10.0	40.0
35	29.78505373656586	35.0	25.0	38.0	8.0	40.0
36	29.149833289976826	34.0	23.0	38.0	7.0	40.0
37	29.201680872298933	35.0	23.0	38.0	7.0	40.0
38	28.993316296776147	34.0	22.0	38.0	7.0	40.0
39	28.617097426323692	34.0	21.0	37.0	7.0	40.0
40	28.47032289546661	33.0	21.0	37.0	7.0	40.0
41	27.870136207444737	33.0	19.0	37.0	7.0	40.0
42	28.40233989121767	33.0	21.0	38.0	7.0	40.0
43	28.4651218147844	34.0	21.0	38.0	7.0	40.0
44	28.456561029810576	34.0	20.0	38.0	7.0	40.0
45	28.58125264602217	34.0	20.0	38.0	7.0	40.0
46	28.360454104161036	34.0	20.0	38.0	7.0	40.0
47	28.407430455311342	34.0	20.0	38.0	7.0	40.0
48	28.177839893768056	33.0	20.0	37.0	7.0	40.0
49	28.007714057880087	33.0	20.0	37.0	7.0	39.0
50	28.18905137661163	34.0	20.0	37.0	7.0	40.0
51	26.85611590299704	31.0	18.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	25.0
10	30.0
11	49.0
12	61.0
13	75.0
14	167.0
15	339.0
16	715.0
17	1267.0
18	2310.0
19	3700.0
20	5494.0
21	7654.0
22	10513.0
23	14482.0
24	19238.0
25	26102.0
26	32946.0
27	35298.0
28	34523.0
29	35053.0
30	37449.0
31	41710.0
32	45933.0
33	51091.0
34	55735.0
35	58382.0
36	41343.0
37	21821.0
38	4546.0
39	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.65930116110428	2.5070263046483277	31.711459890129507	43.12221264411788
2	42.913591330058644	11.713058130025317	32.244149846892014	13.129200693024023
3	12.866851314382288	11.68840442950487	62.426570228191245	13.018174027921592
4	11.359915123260002	2.87700183797588	70.00817822755195	15.754904811212164
5	18.539242740335325	3.452878277029382	61.95049876986536	16.057380212769935
6	13.462280688331319	10.927030147225098	64.06527619795732	11.54541296648627
7	50.30647100129729	1.3049458723754435	45.70490030553586	2.6836828207914007
8	51.05798380336889	8.221924110809033	37.290507305146505	3.429584780675579
9	47.097409321139104	3.0512779968273236	37.36582861087449	12.485484071159082
10	25.764817299076594	19.89468619239748	42.588162483188725	11.752334025337204
11	22.446089157982613	15.119179388826263	49.54781712735081	12.88691432584031
12	18.22656580752771	13.913358395095104	51.85472339398144	16.00535240339575
13	17.08331420546224	14.478863277377934	56.4885989387007	11.949223578459128
14	14.479203328419596	17.489675200247557	51.57877197367324	16.4523494976596
15	11.910457759709733	14.70652744977021	57.88535859232471	15.497656198195351
16	14.13354144457083	14.930281035183382	50.564059665355764	20.372117854890018
17	14.569656905501516	15.50275696382027	54.2211385929028	15.70644753777542
18	14.702616862791103	14.35440459612988	53.247742486147175	17.695236054931847
19	14.51541876435653	16.619314559115324	50.549777521605996	18.315489154922155
20	16.542633049220687	16.424805363285028	54.40986692102484	12.622694666469435
21	16.599591598698964	19.422525321050692	50.2738261012978	13.704056978952542
22	14.79919135862293	14.15377448154968	53.44361188614412	17.603422273683282
23	15.016824025286196	19.383929527822126	51.640491237734786	13.958755209156894
24	15.060010507577188	16.043438120061822	51.172410978887925	17.72414039347306
25	13.848408646137786	20.595191338219866	48.75719845548817	16.799201560154177
26	14.06281082790527	15.868991935689547	52.13764586064368	17.930551375761503
27	14.879613429975839	17.116809233065883	49.938365748698885	18.065211588259398
28	12.680503343551866	17.486274689830942	52.81128697417482	17.021934992442368
29	15.87001208881453	15.90996808620974	49.61684748880807	18.60317233616766
30	14.358825259671477	18.48330434398203	50.98215242107841	16.17571797526809
31	17.66633171639063	17.114088824732594	47.44035079665458	17.7792286622222
32	17.127860891919877	20.943573630401925	46.39197343521263	15.536592042465575
33	15.442567929446211	19.301297124698415	44.876195917007145	20.37993902884823
34	16.965486519526582	21.146924153315414	43.30039938994843	18.587189937209576
35	14.794090592998007	21.609903646537347	41.69603857539016	21.89996718507448
36	18.876913424705048	21.141483336648832	40.36507879832763	19.616524440318493
37	16.376688140889947	24.537743115241597	40.33260392384897	18.752964820019486
38	19.90233734083486	24.651490188677318	38.25659231450641	17.189580155981414
39	18.18065891690343	25.999962594385416	38.23261871606928	17.586759772641873
40	17.87019231586661	23.57896920327741	38.7608880092902	19.78995047156578
41	17.579788726287816	24.423655990764214	39.41837669834242	18.57817858460555
42	17.252659624209592	22.00334270173953	40.49633850040891	20.247659173641964
43	18.418184569503882	21.205072881439506	39.78563182333668	20.59111072571993
44	19.709698425733702	21.996371655385474	38.20881514315298	20.085114775727835
45	18.177428432007645	21.11087874289931	38.426957886378744	22.2847349387143
46	19.348394193968517	23.468622640258303	37.90089892492863	19.28208424084455
47	15.468921885174966	22.298336980380757	43.6438509420264	18.58889019241788
48	18.11162855544617	23.542583741819648	38.022807223364225	20.322980479369953
49	16.702797089843184	20.983529627797132	41.323512659250156	20.99016062310953
50	17.249939215876303	20.612193890302937	40.68608698165595	21.451779912164817
51	17.450739355977333	20.820815204362177	38.824477554080865	22.903967885579625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	554.5
2	1010.0
3	31369.0
4	61728.0
5	41692.0
6	21656.0
7	20227.5
8	18799.0
9	17982.0
10	17165.0
11	16190.5
12	15216.0
13	14014.0
14	12812.0
15	11704.5
16	10597.0
17	9482.5
18	8368.0
19	7705.0
20	7042.0
21	6530.0
22	6018.0
23	5649.5
24	5281.0
25	5243.5
26	5516.5
27	5827.0
28	6375.0
29	6923.0
30	7560.5
31	8198.0
32	8736.0
33	9274.0
34	10546.5
35	11819.0
36	12586.5
37	13354.0
38	13998.0
39	14642.0
40	15751.0
41	16860.0
42	17898.5
43	18937.0
44	20625.0
45	22313.0
46	39722.0
47	57131.0
48	48045.5
49	38960.0
50	36489.0
51	34018.0
52	30640.5
53	27263.0
54	25138.5
55	23014.0
56	21676.0
57	20338.0
58	18686.5
59	17035.0
60	15668.5
61	14302.0
62	12773.5
63	11245.0
64	9747.5
65	8250.0
66	7103.5
67	5957.0
68	4919.0
69	3881.0
70	3261.5
71	2642.0
72	2257.5
73	1873.0
74	1647.0
75	1151.5
76	882.0
77	653.0
78	424.0
79	303.5
80	183.0
81	135.5
82	88.0
83	63.5
84	39.0
85	34.5
86	30.0
87	24.5
88	19.0
89	12.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.399058368599654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.58131332444472	35.40552178754592
2	6.476003296612102	5.621048901298248
3	2.743171680631954	3.5715320364850727
4	1.6844336230237817	2.9241133249456355
5	1.2343571890420157	2.6784969847467517
6	0.9731270121817454	2.5339677601041934
7	0.8194863450122698	2.4895455003620524
8	0.7015149000962846	2.435606887657681
9	0.5867791989363105	2.2919098233705375
>10	3.0762388667656606	20.38881084124693
>50	0.07374611033568025	2.2773803585872656
>100	0.04384903856746466	3.6807615087056456
>500	0.002391765740043527	0.7411347200270251
>1k	0.001993138116702939	1.5116795479916303
>5k	7.972552466811757E-4	2.3804887365947396
>10k+	7.972552466811757E-4	9.068001280330659
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41478	7.052318553014808	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCG	10938	1.8597391468459414	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGC	7961	1.3535731713330172	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	5799	0.9859779952970941	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTT	2267	0.38544785572314405	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	1935	0.3289993828073594	No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	1729	0.29397412551623997	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTC	1427	0.24262641822537565	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTA	1380	0.2346352187463338	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	944	0.1605040916641588	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCCGAAACTTCGTATGCCG	889	0.15115268801847156	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	637	0.10830625676914105	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	629	0.10694605260249564	No Hit
GAATCTTTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	594	0.10099515937342196	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGT	591	0.10048508281092992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10507577187335819	0.0
2	0.0	0.0	0.0	0.6160024619695417	0.0
3	0.0	0.0	0.0	0.7501525979049455	0.0
4	0.0	0.0	0.0	1.3103866890420253	0.0
5	0.0	0.0	0.0	2.6639598603750425	0.0
6	0.0	0.0	0.0	2.9009754364130056	0.0
7	0.0	0.0	0.0	3.2508879582825383	0.0
8	0.0	0.0	0.0	3.724239008275142	0.0
9	0.0	0.0	0.0	3.8570289400439006	0.0
10	0.0	0.0	0.0	6.334810855109352	0.0
11	0.0	0.0	0.0	6.759874657186043	0.0
12	0.0	0.0	0.0	8.78657886548771	0.0
13	0.0	0.0	0.0	9.003191379025992	0.0
14	0.0	0.0	0.0	9.126629907149063	0.0
15	0.0	0.0	0.0	9.564445623288055	0.0
16	0.0	0.0	0.0	9.823734542554838	0.0
17	0.0	0.0	0.0	10.036096418072352	0.0
18	0.0	0.0	0.0	10.240637119631657	0.0
19	0.0	0.0	0.0	10.911217773787845	0.0
20	0.0	0.0	0.0	11.132591001909386	0.0
21	0.0	0.0	0.0	11.369096501384858	0.0
22	0.0	0.0	0.0	11.71917904877522	0.0
23	0.0	0.0	0.0	11.943102659709222	0.0
24	0.0	0.0	0.0	12.139482136268654	0.0
25	0.0	0.0	0.0	12.300156253453643	0.0
26	0.0	0.0	0.0	12.473072208138442	0.0
27	0.0	0.0	0.0	12.647178341469054	0.0
28	0.0	0.0	0.0	12.807172356570721	0.0
29	1.7002552083067668E-4	0.0	0.0	12.999131169588555	0.0
30	1.7002552083067668E-4	0.0	0.0	13.201631564897891	0.0
31	1.7002552083067668E-4	0.0	0.0	13.372677238853552	0.0
32	1.7002552083067668E-4	0.0	0.0	13.555964750309021	0.0
33	3.4005104166135336E-4	0.0	0.0	13.727180449785513	0.0
34	3.4005104166135336E-4	0.0	0.0	13.913868471657596	0.0
35	3.4005104166135336E-4	0.0	0.0	14.12827065342508	0.0
36	3.4005104166135336E-4	0.0	0.0	14.302546812276523	0.0
37	3.4005104166135336E-4	0.0	0.0	14.476482920086305	0.0
38	3.4005104166135336E-4	0.0	0.0	14.689354872166312	0.0
39	3.4005104166135336E-4	0.0	0.0	15.021414714348623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAGT	25	3.8884733E-5	45.000004	13
CCCGTTG	20	7.0304744E-4	45.0	1
AGATTCG	20	7.0304744E-4	45.0	34
ATAAGCG	20	7.0304744E-4	45.0	34
CGTTTTT	19010	0.0	44.372696	1
TACGGCT	1075	0.0	43.534885	7
GATACCT	1545	0.0	43.398056	5
ACGGCTG	1095	0.0	42.739723	8
TGATACC	1590	0.0	42.31132	4
CGACCAC	855	0.0	41.842102	12
GAATGAT	4380	0.0	41.712326	1
ATGATAC	4215	0.0	41.263344	3
ATACCTG	1625	0.0	41.26154	6
GGGCAAC	60	3.6379788E-12	41.249996	7
AATGATA	4175	0.0	41.227547	2
ACACGAC	115	0.0	41.08696	26
CACGACC	115	0.0	41.08696	27
ACCACCG	515	0.0	41.06796	14
ATACGGC	2630	0.0	40.979084	6
CGGTAGG	50	1.0804797E-9	40.500004	31
>>END_MODULE