##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933430.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	222276
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.168110817182242	31.0	28.0	31.0	25.0	34.0
2	28.032018751462147	31.0	25.0	33.0	16.0	34.0
3	27.967112958664003	31.0	25.0	33.0	16.0	34.0
4	32.92548903165434	35.0	32.0	37.0	28.0	37.0
5	34.21239809965988	35.0	33.0	37.0	30.0	37.0
6	33.22581385304756	35.0	32.0	37.0	28.0	37.0
7	33.776125177706994	35.0	33.0	37.0	30.0	37.0
8	33.17776997966492	35.0	32.0	37.0	28.0	37.0
9	34.862310820781374	37.0	34.0	39.0	29.0	39.0
10	34.80548507261243	37.0	33.0	39.0	28.0	39.0
11	35.1248582842952	37.0	34.0	39.0	29.0	39.0
12	35.14776674044881	37.0	34.0	39.0	28.0	39.0
13	35.17602890100596	37.0	34.0	39.0	27.0	39.0
14	36.0438238946175	38.0	34.0	40.0	29.0	41.0
15	36.11230182295884	38.0	34.0	40.0	27.0	41.0
16	36.33290143785204	38.0	34.0	40.0	30.0	41.0
17	35.39847756842844	37.0	33.0	40.0	27.0	41.0
18	35.29343248933758	37.0	33.0	39.0	27.0	40.0
19	34.54279364393817	37.0	33.0	37.0	27.0	40.0
20	33.31950817902068	35.0	32.0	37.0	25.0	39.0
21	34.180910219726826	35.0	33.0	38.0	27.0	40.0
22	34.68536864078893	35.0	33.0	38.0	29.0	40.0
23	34.83269898684518	35.0	33.0	39.0	29.0	40.0
24	34.49575302776728	35.0	33.0	38.0	27.0	40.0
25	33.570565423167594	35.0	33.0	38.0	24.0	40.0
26	33.51017203836672	35.0	32.0	38.0	24.0	40.0
27	34.16052115388076	35.0	33.0	38.0	26.0	40.0
28	33.762763411254475	35.0	33.0	38.0	24.0	40.0
29	33.688351418956614	35.0	33.0	38.0	24.0	40.0
30	33.10705159351437	35.0	32.0	38.0	21.0	40.0
31	32.977757382713385	35.0	32.0	38.0	21.0	40.0
32	32.34446813871043	35.0	31.0	38.0	18.0	40.0
33	31.5188819305728	35.0	30.0	39.0	12.0	40.0
34	30.918115316093505	35.0	28.0	39.0	10.0	40.0
35	30.47094153214922	35.0	25.0	39.0	8.0	40.0
36	29.749464629559647	35.0	22.0	39.0	7.0	40.0
37	29.571019813205204	35.0	21.0	39.0	7.0	40.0
38	29.028599578901904	35.0	21.0	38.0	7.0	40.0
39	28.822868865734492	35.0	19.0	38.0	7.0	40.0
40	28.49409292951106	34.0	18.0	38.0	7.0	40.0
41	28.05784250211449	34.0	18.0	38.0	7.0	40.0
42	28.223047022620527	34.0	18.0	38.0	7.0	40.0
43	28.190146484550738	34.0	17.0	38.0	7.0	40.0
44	28.2827880652882	35.0	17.0	39.0	7.0	40.0
45	28.19563965520344	35.0	17.0	39.0	7.0	40.0
46	27.97334844967518	34.0	16.0	38.0	7.0	40.0
47	27.77984577732189	34.0	17.0	38.0	7.0	40.0
48	27.674400295128578	34.0	17.0	38.0	7.0	40.0
49	27.907097482409256	34.0	16.0	38.0	7.0	40.0
50	27.767874174449783	34.0	15.0	38.0	7.0	40.0
51	26.624435386636435	32.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	15.0
11	11.0
12	14.0
13	18.0
14	31.0
15	38.0
16	131.0
17	250.0
18	498.0
19	933.0
20	1435.0
21	2188.0
22	3074.0
23	4399.0
24	6498.0
25	9375.0
26	12637.0
27	13488.0
28	12263.0
29	11328.0
30	10889.0
31	12104.0
32	13654.0
33	15615.0
34	17780.0
35	19305.0
36	19966.0
37	18675.0
38	13604.0
39	2050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.24886177545034	2.008763879141248	24.31166657668844	39.43070776871997
2	39.18191797584984	25.318972808580327	25.221346434162932	10.2777627814069
3	9.867911965304396	25.32167215533841	54.45707138872393	10.353344490633267
4	8.866004426928683	2.1473303460562545	77.06455037880832	11.922114848206734
5	26.91428674260829	2.6314131980060824	55.77930140905901	14.674998650326621
6	10.893663733376522	21.86830786949558	58.807068689377175	8.430959707750723
7	41.640123090212164	1.1220284691104752	54.60373589591319	2.634112544764167
8	39.22870665298998	17.79319404704062	38.86024582051144	4.117853479457971
9	37.017491766992386	2.603070057046195	37.28112796703198	23.09831020892944
10	28.998632330975905	14.527434360884667	43.13421152081197	13.339721787327466
11	25.259137288776117	12.467382893339812	48.877521639763174	13.395958178120896
12	16.953247314149973	11.268423041623928	51.21875506127517	20.559574582950926
13	16.304054418830642	13.411254476416707	60.97059511598193	9.314095988770719
14	13.899836239630009	22.403678309849017	47.75459338840001	15.941892062120965
15	8.596069751120229	13.246144433047203	63.666342745055694	14.49144307077687
16	10.171138584462561	15.469056488329825	48.612985657470894	25.746819269736726
17	10.432525328870414	17.016232071838616	56.338066187982506	16.213176411308464
18	10.836977451456747	15.529341899260379	53.75119221148482	19.882488437798052
19	11.190141985639475	18.23273767748205	49.904623081214346	20.67249725566413
20	14.073494214400114	16.23522107649949	58.17947056812252	11.511814140977883
21	13.892188090482103	24.716568590401124	49.070974824092566	12.320268495024205
22	11.420486242329355	13.272688009501701	54.46696899350357	20.83985675466537
23	12.508772876963775	23.742104410732605	50.55111662977559	13.198006082528027
24	15.492000935773543	15.451060843275927	48.0258777375875	21.031060483363024
25	9.914700642444528	28.028667062570854	47.10270114632259	14.953931148662022
26	12.518220590617071	14.36277420864151	53.48665622919253	19.632348971548886
27	16.699958610016377	19.103726898090663	46.469254440425416	17.727060051467543
28	10.535100505677626	17.611887923122605	56.27193219240944	15.581079378790333
29	20.989670499739063	13.322176033399918	47.809929996940745	17.87822346992028
30	15.403372383883102	22.306501826557973	50.2438409904803	12.046284799078624
31	18.921520991919955	14.449153304900214	47.076157569868094	19.553168133311736
32	20.34902553582033	26.47879213230398	41.158289693894076	12.01389263798161
33	14.94223397937699	19.405603843869784	40.56263384261009	25.089528334143136
34	27.049703971638863	19.791610430275874	39.34837769259839	13.810307905486871
35	19.062336914466698	23.857276539077542	37.310370890244556	19.770015656211196
36	21.169177059151686	19.159063506631398	40.138836401590815	19.532923032626105
37	19.824452482499236	25.448091561842034	40.074052079396786	14.653403876261944
38	16.312152459104897	26.516582986917165	38.18046032860048	18.99080422537746
39	23.382641400781008	25.96051755475175	35.23592290665659	15.420918137810649
40	16.078658964530586	30.78829923158596	36.71111590994979	16.42192589393367
41	20.998668322266013	23.456873436628335	33.55018085623279	21.99427738487286
42	15.345336428584282	27.20221706347064	40.65486152351131	16.797584984433765
43	20.40346236210837	21.573179290611673	32.255844085731255	25.76751426154871
44	22.59128290953589	21.254656373157697	36.054274865482554	20.09978585182386
45	16.189332181612052	21.29244722777088	37.111519012399	25.406701578218073
46	24.13171012614947	26.122028469110475	31.82124925767964	17.92501214706041
47	15.016915906350665	20.99551908438158	45.57802011913117	18.409544890136587
48	21.073350249239684	23.053770987421043	33.17767100361713	22.695207759722148
49	16.14074393996653	19.834799978405226	43.068977307491586	20.955478774136658
50	19.059637567708617	18.706922924652233	37.701776170166816	24.53166333747233
51	18.994853245514584	18.929169141067863	35.692562399899224	26.38341521351833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26.0
1	310.0
2	594.0
3	7746.5
4	14899.0
5	11494.5
6	8090.0
7	8097.5
8	8105.0
9	7986.5
10	7868.0
11	7434.5
12	7001.0
13	6391.5
14	5782.0
15	5204.5
16	4627.0
17	4187.0
18	3747.0
19	3471.5
20	3196.0
21	2953.0
22	2710.0
23	2552.0
24	2394.0
25	2393.0
26	2329.0
27	2266.0
28	2349.5
29	2433.0
30	2464.5
31	2496.0
32	2689.0
33	2882.0
34	3073.0
35	3264.0
36	3385.0
37	3506.0
38	3790.0
39	4074.0
40	4796.0
41	5518.0
42	5946.0
43	6374.0
44	7193.0
45	8012.0
46	26803.0
47	45594.0
48	30548.0
49	15502.0
50	13604.0
51	11706.0
52	9605.5
53	7505.0
54	6893.5
55	6282.0
56	6042.0
57	5802.0
58	5096.0
59	4390.0
60	3984.0
61	3578.0
62	3365.0
63	3152.0
64	2629.0
65	2106.0
66	1806.5
67	1507.0
68	1264.0
69	1021.0
70	856.0
71	691.0
72	586.5
73	482.0
74	412.5
75	261.0
76	179.0
77	129.0
78	79.0
79	68.0
80	57.0
81	44.5
82	32.0
83	19.0
84	6.0
85	5.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	222276.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.26243049182098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.23988685759447	37.30947110799187
2	4.916703063580209	4.254170490741241
3	1.7605707036043343	2.284997030718566
4	1.13974334976394	1.9723226979071065
5	0.812171127888355	1.7568248483866904
6	0.6489049728583016	1.6843923770447553
7	0.5573927330962334	1.6879915060555344
8	0.503317318691375	1.7419784412172254
9	0.46068093425677503	1.7937159207471791
>10	2.8431189035169817	19.99136209037413
>50	0.07175391526798527	2.0901941730101314
>100	0.03327717809529752	2.549083121884504
>500	0.002079823630956095	0.5551656499127211
>1k	0.006239470892868285	4.956900430095916
>5k	0.0031197354464341425	10.835177886951358
>10k+	0.0010399118154780474	4.536252226961075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	10083	4.536252226961075	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	9680	4.354946103043064	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	7618	3.427270600514675	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6786	3.0529611833936188	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	2241	1.008206014144577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	1871	0.8417462973960301	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	1770	0.7963072936349405	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	1737	0.7814608864654753	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	1733	0.7796613219600856	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1666	0.7495186164948082	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGT	726	0.3266209577282298	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTAT	508	0.22854469218449133	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATG	439	0.19750220446651912	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	389	0.1750076481491479	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAAGAC	311	0.13991614029404884	No Hit
GAATGATACGGCGACCACCCGTCTCTTATACACATCTGACGCGGTAAGACT	275	0.12372005974554158	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	240	0.10797387032338174	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	238	0.10707408807068688	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12057082186110961	0.0
2	0.0	0.0	0.0	1.046446759884108	0.0
3	0.0	0.0	0.0	1.1652180172398279	0.0
4	0.0	0.0	0.0	2.1347333945185265	0.0
5	0.0	0.0	0.0	6.170706688981267	0.0
6	0.0	0.0	0.0	6.469884288002303	0.0
7	0.0	0.0	0.0	7.453346290197772	0.0
8	0.0	0.0	0.0	8.436358401266894	0.0
9	0.0	0.0	0.0	8.584822472961543	0.0
10	0.0	0.0	0.0	14.061347153988734	0.0
11	0.0	0.0	0.0	14.357825406251687	0.0
12	0.0	0.0	0.0	19.609404524105166	0.0
13	0.0	0.0	0.0	19.97516600982562	0.0
14	0.0	0.0	0.0	20.295488491784987	0.0
15	0.0	0.0	0.0	21.392772948946355	0.0
16	0.0	0.0	0.0	21.899350357213553	0.0
17	0.0	0.0	0.0	22.128344940524393	0.0
18	0.0	0.0	0.0	22.28130792348252	0.0
19	0.0	0.0	0.0	23.66202379024276	0.0
20	0.0	0.0	0.0	23.960301607011104	0.0
21	0.0	0.0	0.0	24.125861541506957	0.0
22	0.0	0.0	0.0	24.540211268872934	0.0
23	0.0	0.0	0.0	24.69812305422088	0.0
24	0.0	0.0	0.0	24.82184311396642	0.0
25	0.0	0.0	0.0	24.919019597257464	0.0
26	0.0	0.0	0.0	25.058035955298816	0.0
27	0.0	0.0	0.0	25.266335546797674	0.0
28	0.0	0.0	0.0	25.361712465583327	0.0
29	0.0	0.0	0.0	25.484982634202524	0.0
30	0.0	0.0	0.0	25.611851931832497	0.0
31	0.0	0.0	0.0	25.730623189188215	0.0
32	0.0	0.0	0.0	25.85884216019723	0.0
33	0.0	0.0	0.0	25.97626374417391	0.0
34	0.0	0.0	0.0	26.176015404272167	0.0
35	0.0	0.0	0.0	26.301535028523098	0.0
36	0.0	0.0	0.0	26.433353128542894	0.0
37	0.0	0.0	0.0	26.57146970433155	0.0
38	0.0	0.0	0.0	26.719033993773508	0.0
39	0.0	0.0	0.0	26.84995231154061	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	30	2.157576E-6	45.000004	4
TTCGGGA	75	0.0	45.000004	4
TCGTTTG	25	3.8806233E-5	45.0	1
GATACCG	20	7.020997E-4	45.0	27
GACACTG	20	7.020997E-4	45.0	29
ACTTGAC	20	7.020997E-4	45.0	26
GTGGGAT	45	3.8198777E-10	45.0	5
ACCGACT	50	2.1827873E-11	45.0	17
GTGATAC	20	7.020997E-4	45.0	3
TTCCGAT	20	7.020997E-4	45.0	13
TACGGGA	20	7.020997E-4	45.0	4
GCACCGA	50	2.1827873E-11	45.0	9
GAAACCG	20	7.020997E-4	45.0	21
CTTGACT	20	7.020997E-4	45.0	27
CGGGATA	25	3.8806233E-5	45.0	6
CGGGAAC	20	7.020997E-4	45.0	6
CAAGCAG	25	3.8806233E-5	45.0	10
AACTAGT	20	7.020997E-4	45.0	43
TATACTA	25	3.8806233E-5	45.0	44
CACCGAC	55	1.8189894E-12	45.0	16
>>END_MODULE