SRR2933429.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153009
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10707	6.997627590533891	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	5455	3.565149762432275	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	4610	3.0128946663268175	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	2556	1.670489971178166	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	1470	0.9607278003254711	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	942	0.6156500598004039	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	743	0.4855923507767517	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	732	0.47840323118247946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	624	0.4078191478932612	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	459	0.29998235397917766	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTAAGACTCGTATGCCG	440	0.2875647837708893	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	328	0.21436647517466292	TruSeq Adapter, Index 15 (95% over 23bp)
GAATCTTTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	318	0.20783091190714273	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGT	299	0.19541334169885433	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGGTAAGACTCGTATGC	211	0.13790038494467646	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	204	0.1333254906574123	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	192	0.12548281473638806	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	191	0.12482925840963603	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATG	189	0.12352214575613199	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	189	0.12352214575613199	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	188	0.12286858942937998	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	175	0.1143723571816037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	173	0.11306524452809966	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTAT	172	0.11241168820134763	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	170	0.11110457554784359	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAAGAC	160	0.10456901228032339	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	158	0.10326189962681932	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	158	0.10326189962681932	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	157	0.10260834330006732	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCTA	25	3.874916E-5	45.000004	13
TCTTGCG	35	1.2027158E-7	45.000004	1
CTCGTAA	25	3.874916E-5	45.000004	33
GGGCTGC	25	3.874916E-5	45.000004	7
GAATGAC	100	0.0	45.000004	1
CCGATGA	50	2.1827873E-11	45.000004	18
CATGCAC	25	3.874916E-5	45.000004	45
GGACTAA	35	1.2027158E-7	45.000004	8
GATCCGG	35	1.2027158E-7	45.000004	5
ACCGACT	25	3.874916E-5	45.000004	17
GAATAGC	25	3.874916E-5	45.000004	27
ACTTACT	25	3.874916E-5	45.000004	38
GCGGGAC	35	1.2027158E-7	45.000004	5
TGCCTTG	25	3.874916E-5	45.000004	1
CGAATAG	25	3.874916E-5	45.000004	26
AACGAAT	25	3.874916E-5	45.000004	24
TCGTAAC	25	3.874916E-5	45.000004	34
TGCTACG	50	2.1827873E-11	45.000004	4
CGATGAA	50	2.1827873E-11	45.000004	19
CGCACTT	25	3.874916E-5	45.000004	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content