##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933429.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153009
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.749060512780293	31.0	25.0	31.0	16.0	33.0
2	27.515054669986732	31.0	25.0	33.0	16.0	34.0
3	27.644328111418282	31.0	25.0	33.0	16.0	34.0
4	31.307073440124437	35.0	28.0	35.0	19.0	37.0
5	33.55194138906862	35.0	33.0	37.0	28.0	37.0
6	31.648465122966623	35.0	31.0	37.0	17.0	37.0
7	32.38149389905169	35.0	32.0	35.0	26.0	37.0
8	32.048624590710354	35.0	32.0	36.0	17.0	37.0
9	33.201582913423394	35.0	32.0	38.0	26.0	39.0
10	32.039755831356324	35.0	28.0	39.0	17.0	39.0
11	32.73287845812992	37.0	30.0	39.0	17.0	39.0
12	33.51992366462103	37.0	31.0	39.0	25.0	39.0
13	33.64133482344176	35.0	32.0	39.0	25.0	39.0
14	33.83244776451058	36.0	32.0	40.0	21.0	41.0
15	34.21122286924299	37.0	32.0	40.0	25.0	41.0
16	34.50567613669784	37.0	32.0	40.0	25.0	41.0
17	32.30218483880033	35.0	27.0	40.0	18.0	41.0
18	33.09045873118575	36.0	30.0	39.0	23.0	39.0
19	32.739329059074954	36.0	30.0	37.0	21.0	39.0
20	31.840355796064284	34.0	30.0	35.0	22.0	39.0
21	32.5186230875308	35.0	31.0	37.0	24.0	39.0
22	32.70487356952859	34.0	30.0	37.0	25.0	39.0
23	33.48923265951676	35.0	32.0	38.0	26.0	39.0
24	32.133567306498314	35.0	31.0	37.0	21.0	39.0
25	31.426327863066877	34.0	30.0	37.0	18.0	39.0
26	30.45178388199387	33.0	25.0	37.0	18.0	39.0
27	32.141070133129425	34.0	30.0	37.0	22.0	39.0
28	32.109921638596425	35.0	30.0	37.0	21.0	39.0
29	32.112607755099376	35.0	30.0	37.0	21.0	40.0
30	31.238999013129945	34.0	29.0	37.0	18.0	39.0
31	31.651700226784044	34.0	30.0	37.0	20.0	39.0
32	31.367952211961388	35.0	30.0	37.0	18.0	39.0
33	31.096981223326733	35.0	30.0	37.0	15.0	40.0
34	30.127253952381885	35.0	26.0	37.0	10.0	40.0
35	29.64396865543857	35.0	24.0	37.0	8.0	40.0
36	28.81799109856283	34.0	22.0	37.0	7.0	40.0
37	28.73444699331412	34.0	21.0	37.0	7.0	40.0
38	28.3644687567398	33.0	21.0	37.0	7.0	40.0
39	28.05178780333183	33.0	20.0	37.0	7.0	39.0
40	27.777810455594114	33.0	18.0	37.0	7.0	39.0
41	27.356501905116694	33.0	18.0	37.0	7.0	39.0
42	27.766007228332974	33.0	18.0	37.0	7.0	40.0
43	27.786613859315466	33.0	18.0	37.0	7.0	40.0
44	27.707291727937573	33.0	18.0	37.0	7.0	40.0
45	27.77450999614402	34.0	18.0	37.0	7.0	40.0
46	27.410394159820665	33.0	17.0	37.0	7.0	40.0
47	27.39754524243672	33.0	18.0	37.0	7.0	40.0
48	27.20641269467809	33.0	17.0	37.0	7.0	39.0
49	27.242469397225	33.0	17.0	37.0	7.0	39.0
50	27.28044755537256	33.0	17.0	37.0	7.0	40.0
51	25.617009456960048	31.0	14.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	7.0
11	16.0
12	11.0
13	22.0
14	40.0
15	95.0
16	209.0
17	377.0
18	575.0
19	1043.0
20	1536.0
21	2079.0
22	2892.0
23	3894.0
24	5404.0
25	7290.0
26	9293.0
27	10063.0
28	9718.0
29	9798.0
30	10427.0
31	11418.0
32	12520.0
33	12996.0
34	13549.0
35	13589.0
36	9029.0
37	4253.0
38	846.0
39	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.632897411263393	1.9881183459796483	26.686665490265277	41.69231875249169
2	43.06805482030469	19.9027508185793	26.418053839970195	10.611140521145815
3	10.327497075335437	19.811252932834016	59.253377252318494	10.607872739512054
4	9.39421864073355	2.207059715441575	76.0216719277951	12.377049716029775
5	23.616257867184284	2.6286035461966293	59.912815586011284	13.842323000607806
6	11.213065898084427	17.30617153239352	61.72251305478763	9.758249514734427
7	41.22306530988374	1.1149670934389482	54.93859838310164	2.7233692135756717
8	40.679306446026054	14.395885209366769	40.20417099647734	4.7206373481298485
9	37.49844780372397	2.4985458371729767	39.787855616336294	20.215150742766767
10	26.568371795123163	14.627897705363738	44.74050546046311	14.063225039049989
11	22.8777392179545	13.120143259546824	51.43292224640381	12.569195276094868
12	16.7820193583384	11.927402963224386	52.89819553098184	18.39238214745538
13	15.725218777980379	13.837094549993791	61.33626126567718	9.101425406348646
14	12.949565058264549	19.483821213131254	50.61140194367651	16.955211784927684
15	8.279251547294605	13.787424269160637	63.326993836963844	14.60633034658092
16	10.032089615643525	13.517505506212052	51.056473802194645	25.393931075949784
17	9.944513067858754	16.023240462979302	57.630596892993225	16.401649576168722
18	10.466051016606865	14.087406623139815	56.09277885614572	19.3537635041076
19	11.462070858576947	17.40681920671333	51.64402093994471	19.487088994765013
20	13.52273395682607	15.354652340711986	59.7899469965819	11.332666705880046
21	13.783502931200125	22.649648059918043	50.64342620368737	12.923422805194464
22	11.086275970694533	13.39659758576293	55.48366435961284	20.033462083929702
23	11.917599618323106	22.38691841656373	51.07085204138318	14.624629923729977
24	14.08609951048631	14.4377128142789	50.91269141030919	20.563496264925593
25	10.043853629525062	23.76004025906973	49.45395368899869	16.742152422406527
26	12.175754367390153	14.92330516505565	55.19087112522792	17.710069342326268
27	14.267134612996621	18.128998947774313	50.02385480592645	17.580011633302618
28	10.2993941532851	16.65653654360201	58.49786613859316	14.546203164519733
29	18.511982955251	13.334509734721486	50.89569894581365	17.25780836421387
30	14.071067714971013	21.559516106895675	52.47991948186055	11.889496696272769
31	17.783921207249247	15.03637040958375	48.73896306753198	18.44074531563503
32	18.45446999849682	24.96650523825396	44.53594233018973	12.043082433059492
33	13.937088667986849	18.11854204654628	44.24118842682457	23.703180858642302
34	23.623446986778557	19.557673078054233	42.028246704442225	14.790633230724989
35	18.11658137756603	21.703298498781116	40.547941624348894	19.63217849930396
36	19.90013659327229	18.06298975877236	43.27719284486534	18.759680803090014
37	18.04926507591057	24.49398401401225	42.88179126718036	14.574959642896824
38	15.62457110366057	25.991281558601127	41.47860583364377	16.905541504094533
39	21.16150030390369	24.83710108555706	38.67550274820435	15.325895862334896
40	15.979452189086915	28.600278414995195	39.11142481814795	16.308844577769936
41	19.780535785476673	21.43599396113954	37.30630224365887	21.477168009724917
42	14.874288440549249	24.730571404296477	42.73866243162167	17.6564777235326
43	18.957054813769126	19.745897300158813	36.771039612048966	24.5260082740231
44	21.724865857563934	19.96483866962074	39.19834780960597	19.111947663209353
45	15.963113280918115	20.58310295472815	40.17345384911999	23.280329915233743
46	21.764079237169057	25.117476749733676	35.34694037605631	17.771503637040958
47	14.716781365802012	20.673947284146685	46.4652406067617	18.144030743289612
48	20.173323137854638	21.924854093550053	36.61745387526224	21.284368893333074
49	15.531112548935031	19.503427902933815	43.76278519564208	21.20267435248907
50	18.201543700043786	18.06625754040612	39.23690763288434	24.49529112666575
51	18.32506584579992	18.158408982478154	38.270951381944855	25.24557378977707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	123.0
2	215.0
3	7897.0
4	15579.0
5	10629.0
6	5679.0
7	5648.5
8	5618.0
9	5592.0
10	5566.0
11	5190.0
12	4814.0
13	4241.5
14	3669.0
15	3333.0
16	2997.0
17	2678.0
18	2359.0
19	2171.0
20	1983.0
21	1869.5
22	1756.0
23	1628.0
24	1500.0
25	1463.0
26	1436.5
27	1447.0
28	1432.0
29	1417.0
30	1436.5
31	1456.0
32	1623.5
33	1791.0
34	1959.0
35	2127.0
36	2193.0
37	2259.0
38	2500.0
39	2741.0
40	3286.0
41	3831.0
42	4209.5
43	4588.0
44	5215.0
45	5842.0
46	14238.0
47	22634.0
48	18136.5
49	13639.0
50	11865.0
51	10091.0
52	8235.5
53	6380.0
54	5432.0
55	4484.0
56	4127.0
57	3770.0
58	3371.5
59	2973.0
60	2679.5
61	2386.0
62	2160.0
63	1934.0
64	1594.5
65	1255.0
66	1088.5
67	922.0
68	798.0
69	674.0
70	548.5
71	423.0
72	373.5
73	324.0
74	266.5
75	161.5
76	114.0
77	81.0
78	48.0
79	40.5
80	33.0
81	25.0
82	17.0
83	11.0
84	5.0
85	3.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	153009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82475540654472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.34736722003538	38.65197472044128
2	6.015745336299846	5.51340117248005
3	2.2961948770608704	3.1566770582122623
4	1.5132066860630955	2.7736930507355777
5	1.0282959666837812	2.356070557941036
6	0.8842489588681615	2.4312295355175184
7	0.7287922870671459	2.3377709807919795
8	0.618974271207713	2.269147566483017
9	0.4535341434194763	1.8704782071642845
>10	1.995264989446061	14.498493552666837
>50	0.0556221119288037	1.8201543700043787
>100	0.049917279936105875	4.126554647112261
>500	0.005704831992697815	1.987464789652896
>1k	0.004278623994523361	5.644112437830454
>5k	0.0014262079981744538	3.565149762432275
>10k+	0.0014262079981744538	6.997627590533891
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10707	6.997627590533891	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	5455	3.565149762432275	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	4610	3.0128946663268175	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	2556	1.670489971178166	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	1470	0.9607278003254711	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	942	0.6156500598004039	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	743	0.4855923507767517	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	732	0.47840323118247946	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	624	0.4078191478932612	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	459	0.29998235397917766	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTAAGACTCGTATGCCG	440	0.2875647837708893	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	328	0.21436647517466292	TruSeq Adapter, Index 15 (95% over 23bp)
GAATCTTTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	318	0.20783091190714273	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGT	299	0.19541334169885433	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCGGTAAGACTCGTATGC	211	0.13790038494467646	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	204	0.1333254906574123	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	192	0.12548281473638806	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	191	0.12482925840963603	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATG	189	0.12352214575613199	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	189	0.12352214575613199	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	188	0.12286858942937998	No Hit
GAATCTGTCTCTTATCCACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	175	0.1143723571816037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	173	0.11306524452809966	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTAT	172	0.11241168820134763	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	170	0.11110457554784359	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAAGAC	160	0.10456901228032339	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	158	0.10326189962681932	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	158	0.10326189962681932	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	157	0.10260834330006732	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2744936572358489	0.0
2	0.0	0.0	0.0	1.4129887784378696	0.0
3	0.0	0.0	0.0	1.6855217666934625	0.0
4	0.0	0.0	0.0	2.6135717506813325	0.0
5	0.0	0.0	0.0	4.968335195968865	0.0
6	0.0	0.0	0.0	5.355894097732813	0.0
7	0.0	0.0	0.0	5.916645426086047	0.0
8	0.0	0.0	0.0	6.654510518989079	0.0
9	0.0	0.0	0.0	6.851884529668189	0.0
10	0.0	0.0	0.0	11.78623479664595	0.0
11	0.0	0.0	0.0	12.648929147958617	0.0
12	0.0	0.0	0.0	16.83691808978557	0.0
13	0.0	0.0	0.0	17.2029096327667	0.0
14	0.0	0.0	0.0	17.40681920671333	0.0
15	0.0	0.0	0.0	18.174094334320202	0.0
16	0.0	0.0	0.0	18.63877288264089	0.0
17	0.0	0.0	0.0	19.050513368494663	0.0
18	0.0	0.0	0.0	19.370755968603152	0.0
19	0.0	0.0	0.0	20.832761471547425	0.0
20	0.0	0.0	0.0	21.27456554843179	0.0
21	0.0	0.0	0.0	21.67780980203779	0.0
22	0.0	0.0	0.0	22.398028874118516	0.0
23	0.0	0.0	0.0	22.78689488853597	0.0
24	0.0	0.0	0.0	23.033939180048232	0.0
25	0.0	0.0	0.0	23.268565901352208	0.0
26	0.0	0.0	0.0	23.463979243051064	0.0
27	0.0	0.0	0.0	23.682267056186237	0.0
28	0.0	0.0	0.0	23.86330215869655	0.0
29	0.0	0.0	0.0	24.06132972570241	0.0
30	0.0	0.0	0.0	24.30053134129365	0.0
31	6.535563267520211E-4	0.0	0.0	24.522086936062585	0.0
32	0.0013071126535040421	0.0	0.0	24.75017809409904	0.0
33	0.0013071126535040421	0.0	0.0	24.952780555392167	0.0
34	0.0013071126535040421	0.0	0.0	25.163225692606318	0.0
35	0.0019606689802560634	0.0	0.0	25.397852413910293	0.0
36	0.0019606689802560634	0.0	0.0	25.58738374866838	0.0
37	0.0019606689802560634	0.0	0.0	25.766458182198434	0.0
38	0.0019606689802560634	0.0	0.0	25.983438882680105	0.0
39	0.0019606689802560634	0.0	0.0	26.200419583161775	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCTA	25	3.874916E-5	45.000004	13
TCTTGCG	35	1.2027158E-7	45.000004	1
CTCGTAA	25	3.874916E-5	45.000004	33
GGGCTGC	25	3.874916E-5	45.000004	7
GAATGAC	100	0.0	45.000004	1
CCGATGA	50	2.1827873E-11	45.000004	18
CATGCAC	25	3.874916E-5	45.000004	45
GGACTAA	35	1.2027158E-7	45.000004	8
GATCCGG	35	1.2027158E-7	45.000004	5
ACCGACT	25	3.874916E-5	45.000004	17
GAATAGC	25	3.874916E-5	45.000004	27
ACTTACT	25	3.874916E-5	45.000004	38
GCGGGAC	35	1.2027158E-7	45.000004	5
TGCCTTG	25	3.874916E-5	45.000004	1
CGAATAG	25	3.874916E-5	45.000004	26
AACGAAT	25	3.874916E-5	45.000004	24
TCGTAAC	25	3.874916E-5	45.000004	34
TGCTACG	50	2.1827873E-11	45.000004	4
CGATGAA	50	2.1827873E-11	45.000004	19
CGCACTT	25	3.874916E-5	45.000004	35
>>END_MODULE