##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933428.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	666946
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.41823326026395	31.0	28.0	33.0	25.0	34.0
2	28.50352202427183	31.0	27.0	33.0	16.0	34.0
3	28.44470167000027	31.0	26.0	33.0	16.0	34.0
4	33.16137438413305	35.0	32.0	37.0	28.0	37.0
5	34.34252248307959	35.0	33.0	37.0	32.0	37.0
6	33.60783331783983	35.0	33.0	37.0	28.0	37.0
7	33.921477900759584	35.0	33.0	37.0	30.0	37.0
8	33.52084426625244	35.0	33.0	37.0	28.0	37.0
9	34.994882644172094	37.0	34.0	39.0	29.0	39.0
10	35.051986217774754	37.0	34.0	39.0	28.0	39.0
11	35.33505711107046	37.0	34.0	39.0	30.0	39.0
12	35.36900588653354	37.0	34.0	39.0	30.0	39.0
13	35.43832334251948	37.0	34.0	39.0	30.0	39.0
14	36.280841327483785	38.0	35.0	40.0	29.0	41.0
15	36.48442752486708	38.0	34.0	41.0	30.0	41.0
16	36.48085002384001	38.0	34.0	41.0	30.0	41.0
17	35.70769297664279	38.0	33.0	40.0	27.0	41.0
18	35.4054960971353	38.0	34.0	39.0	27.0	40.0
19	34.64288713029241	37.0	33.0	37.0	27.0	40.0
20	33.66114048213798	35.0	32.0	37.0	27.0	39.0
21	34.15566177771514	35.0	33.0	38.0	27.0	40.0
22	34.58210859649807	35.0	33.0	38.0	29.0	40.0
23	34.8369478188639	35.0	33.0	39.0	30.0	40.0
24	34.35264474185316	35.0	33.0	38.0	27.0	40.0
25	33.485782956941044	35.0	33.0	38.0	24.0	40.0
26	33.31209573188834	35.0	32.0	38.0	24.0	40.0
27	34.1132415517898	35.0	33.0	38.0	26.0	40.0
28	33.765069435906355	35.0	33.0	38.0	24.0	40.0
29	33.709958527377026	35.0	33.0	39.0	24.0	40.0
30	33.047012801636114	35.0	33.0	38.0	21.0	40.0
31	32.92949354220582	35.0	33.0	38.0	21.0	40.0
32	32.10753494285894	35.0	31.0	38.0	16.0	40.0
33	31.259401810641343	35.0	30.0	39.0	11.0	40.0
34	30.429830900852544	35.0	25.0	39.0	8.0	40.0
35	29.834338911995875	35.0	22.0	39.0	7.0	40.0
36	29.156466940351994	35.0	20.0	39.0	7.0	40.0
37	28.99265007961664	35.0	18.0	39.0	7.0	40.0
38	28.810362458130044	35.0	18.0	39.0	7.0	40.0
39	28.267946730319995	35.0	18.0	38.0	7.0	40.0
40	28.047937914014028	34.0	17.0	38.0	7.0	40.0
41	27.47576865293442	33.0	15.0	38.0	7.0	40.0
42	27.675186596815934	34.0	15.0	38.0	7.0	40.0
43	27.54894099372363	34.0	15.0	38.0	7.0	40.0
44	27.660926071975844	34.0	15.0	38.0	7.0	40.0
45	27.81985498076306	34.0	15.0	39.0	7.0	40.0
46	27.57846662248518	34.0	15.0	38.0	7.0	40.0
47	27.49341925733118	34.0	15.0	38.0	7.0	40.0
48	27.433712774347548	34.0	15.0	38.0	7.0	40.0
49	27.575634309224434	34.0	15.0	38.0	7.0	40.0
50	27.413661675757858	34.0	15.0	38.0	7.0	40.0
51	26.263779976189976	31.0	13.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	18.0
9	34.0
10	33.0
11	37.0
12	43.0
13	72.0
14	111.0
15	171.0
16	327.0
17	711.0
18	1471.0
19	2639.0
20	4297.0
21	6596.0
22	9781.0
23	14053.0
24	21037.0
25	30594.0
26	39837.0
27	41628.0
28	37721.0
29	33573.0
30	32830.0
31	34333.0
32	38015.0
33	44030.0
34	49572.0
35	58001.0
36	59992.0
37	57353.0
38	41894.0
39	6137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.379278082483438	2.0055596704980614	25.867911345146382	44.747250901872114
2	43.31115262704927	18.508544919678656	27.077304609368674	11.102997843903404
3	10.27834337412624	18.632542964497876	59.57948619528418	11.509627466091708
4	9.505117355827908	2.4070914286913783	75.1138772854174	12.973913930063302
5	21.85394319780012	2.8258659621618545	60.7501956680151	14.569995172022923
6	11.317258068869144	16.115697522737975	63.50559115730508	9.061453251087794
7	42.52488207441082	1.1545162576880288	53.727888014921746	2.592713652979402
8	40.889517292254546	12.912289750594502	42.61799306090748	3.5801998962434736
9	38.507615309185454	2.5462331283192343	41.69722886110719	17.24892270138812
10	26.56856777010433	14.552302585216795	47.18657882347296	11.692550821205915
11	22.87096706479985	12.766700752384752	52.14350187271533	12.218830310100067
12	16.060670579027388	11.183664044765242	54.69198405867941	18.063681317527955
13	16.17837126244104	12.214032320457727	61.62253015986302	9.985066257238218
14	13.802916577953836	18.424730038114028	53.375385713386095	14.39696767054604
15	9.62821577758919	12.501012075940181	64.00683113775328	13.863941008717347
16	11.855382594692824	14.634917969370834	52.322077049716164	21.187622386220173
17	12.897595907314836	15.192684265292842	57.6950757632552	14.214644064137127
18	12.414198450849094	13.738743466487541	56.24158477597887	17.605473306684498
19	12.365618805720404	16.052873845858585	53.432811651917845	18.148695696503164
20	13.707106722283363	16.101303553810954	59.190399222725674	11.001190501180005
21	14.095294071783924	20.760001559346634	53.42396535851478	11.720739010354661
22	11.930501120030707	12.041904442038785	57.88624566306717	18.141348774863335
23	12.888299802382802	19.728133911890918	55.23250757932426	12.151058706402019
24	14.225589478008715	15.370059944883094	52.39239758541171	18.01195299169648
25	11.695099753203408	23.2759473780486	50.679065471567405	14.34988739718058
26	11.90081355911873	13.202718061132385	56.3442917417602	18.552176637988683
27	14.580340837189217	16.758778071987837	51.7872511417716	16.873629949051345
28	9.940684853046573	16.414222440797303	57.16519778212928	16.479894924026834
29	15.604261814299807	13.986739556125983	54.49721566663568	15.911782962938528
30	12.873605959103134	21.327963583258615	52.82916458004097	12.969265877597286
31	16.159479178224323	14.371178476218466	49.628605614247626	19.84073673130958
32	20.751455140296216	20.116021387038828	46.68353959690889	12.448983875756058
33	17.802040944844112	16.22515166145385	44.71396484872838	21.258842544973653
34	17.71087914163965	19.103345698152474	49.14895658718997	14.036818573017907
35	16.062319887966943	26.54847618847703	40.466694455023344	16.922509468532684
36	19.812998353689803	19.52646840973632	41.37726292683366	19.283270309740217
37	19.04217133021264	25.055701661004036	41.149658293175165	14.752468715608162
38	22.225937332257782	21.56141576679371	35.79165329726845	20.420993603680056
39	22.751767009622966	20.2020853262483	41.265709667649254	15.780437996479474
40	20.872604378765296	24.69675206088649	38.5578442632537	15.872799297094517
41	20.98565700971293	23.00456108890375	38.75861014235035	17.251171759032964
42	17.622116333256365	24.784465309035514	40.96103732536067	16.632381032347446
43	23.61105696713077	20.02995744782936	36.941371565314135	19.417614019725736
44	20.352172439747747	21.992185274370037	38.095587948649516	19.5600543372327
45	16.589049188390064	21.115502604408753	37.89332269778963	24.402125509411558
46	23.752297787227153	22.437798562402353	35.78940423962359	18.020499410746897
47	16.41977011632126	20.07853709295805	45.58944802127908	17.912244769441603
48	18.39309329390985	23.086426787176173	37.014241033007174	21.506238885906807
49	17.065999346273912	20.732712993255824	42.47735198951639	19.72393567095387
50	19.591091332731587	19.123587216956096	39.57306888413755	21.712252566174772
51	17.479376141396756	19.887367193146073	38.05450516233698	24.57875150312019
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	1232.5
2	2406.0
3	29644.5
4	56883.0
5	41954.0
6	27025.0
7	25642.5
8	24260.0
9	23780.0
10	23300.0
11	21989.5
12	20679.0
13	19449.0
14	18219.0
15	16660.5
16	15102.0
17	13864.0
18	12626.0
19	11604.5
20	10583.0
21	9848.0
22	9113.0
23	8655.5
24	8198.0
25	8049.5
26	8123.0
27	8345.0
28	8463.0
29	8581.0
30	8828.5
31	9076.0
32	9769.5
33	10463.0
34	11209.5
35	11956.0
36	11999.5
37	12043.0
38	13075.5
39	14108.0
40	14925.5
41	15743.0
42	16714.0
43	17685.0
44	20834.5
45	23984.0
46	63844.5
47	103705.0
48	71605.0
49	39505.0
50	35208.0
51	30911.0
52	26587.0
53	22263.0
54	21386.0
55	20509.0
56	19599.0
57	18689.0
58	17686.5
59	16684.0
60	14765.5
61	12847.0
62	11382.0
63	9917.0
64	8678.0
65	7439.0
66	6519.0
67	5599.0
68	4697.5
69	3796.0
70	3154.5
71	2513.0
72	2104.5
73	1696.0
74	1419.5
75	912.5
76	682.0
77	520.0
78	358.0
79	282.0
80	206.0
81	152.0
82	98.0
83	60.5
84	23.0
85	17.5
86	12.0
87	11.5
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.64816290619601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.26064955858081	38.91992020538186
2	5.35253585235939	4.886668570995123
3	2.1378131190370757	2.9276172456242233
4	1.3670013088667954	2.4960439376053856
5	1.0268748549304256	2.3437475331070234
6	0.7978612088338018	2.185253906242792
7	0.6594583950355779	2.1072144972509834
8	0.5764072553043083	2.10495458323555
9	0.4974187823440164	2.0435628248137183
>10	2.194716051900217	15.506634804085836
>50	0.07114508959339783	2.280058586750643
>100	0.05043618968582775	4.099805723920884
>500	0.0033401450123064697	0.991217115222197
>1k	0.0030061305110758232	4.277282530891908
>5k	0.0	0.0
>10k+	0.001336058004922588	12.830017934871899
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28151	4.220881450672168	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	19872	2.979551567893053	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	19406	2.9096808437264787	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	16718	2.50664971376993	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	4634	0.6948088750813409	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	4570	0.6852128957966612	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	4258	0.6384324967838475	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	3979	0.596600024589697	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	3346	0.5016897919771616	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	3286	0.4926935613977743	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGT	1750	0.26239005856546105	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTAT	1182	0.17722574241392858	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	1048	0.1571341607866304	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	821	0.12309842176128202	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTT	785	0.11770068341364968	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	766	0.1148518770635104	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	686	0.10285690295766074	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10630545801309252	0.0
2	0.0	0.0	0.0	0.7456375778548788	0.0
3	0.0	0.0	0.0	0.8507435384573864	0.0
4	0.0	0.0	0.0	1.6725492018844104	0.0
5	0.0	0.0	0.0	4.803087506334846	0.0
6	0.0	0.0	0.0	5.0251444644693875	0.0
7	0.0	0.0	0.0	5.691465276049335	0.0
8	0.0	0.0	0.0	6.492429671967446	0.0
9	0.0	0.0	0.0	6.599035004333184	0.0
10	0.0	0.0	0.0	10.262000221907021	0.0
11	0.0	0.0	0.0	10.534136196933485	0.0
12	0.0	0.0	0.0	14.174310963706207	0.0
13	0.0	0.0	0.0	14.47043688694437	0.0
14	0.0	0.0	0.0	14.723380903401475	0.0
15	0.0	0.0	0.0	15.613707856408165	0.0
16	0.0	0.0	0.0	16.02138703883073	0.0
17	0.0	0.0	0.0	16.216605242403432	0.0
18	0.0	0.0	0.0	16.36114468037892	0.0
19	0.0	0.0	0.0	17.36782288221235	0.0
20	0.0	0.0	0.0	17.58718097117308	0.0
21	0.0	0.0	0.0	17.75840922653408	0.0
22	0.0	0.0	0.0	18.094118564321548	0.0
23	0.0	0.0	0.0	18.258599646748014	0.0
24	0.0	0.0	0.0	18.403438959076148	0.0
25	0.0	0.0	0.0	18.52998593589286	0.0
26	0.0	0.0	0.0	18.673475813634088	0.0
27	0.0	0.0	0.0	18.85954784945108	0.0
28	0.0	0.0	0.0	18.984145642975594	0.0
29	0.0	0.0	0.0	19.13633187694356	0.0
30	0.0	0.0	0.0	19.316706300060275	0.0
31	0.0	0.0	0.0	19.465743853325456	0.0
32	0.0	0.0	0.0	19.646718025147464	0.0
33	0.0	0.0	0.0	19.80610124357894	0.0
34	0.0	0.0	0.0	20.077937344252756	0.0
35	0.0	0.0	0.0	20.292647380747468	0.0
36	0.0	0.0	0.0	20.468373751398165	0.0
37	0.0	0.0	0.0	20.65654490768368	0.0
38	0.0	0.0	0.0	20.880251174757777	0.0
39	0.0	0.0	0.0	21.229005046885355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	20	7.031155E-4	45.0	13
CTCGTAG	25	3.889038E-5	45.0	1
TCGTACA	20	7.031155E-4	45.0	34
TATCGTG	25	3.889038E-5	45.0	1
TGCACGG	35	1.2109012E-7	45.0	2
ACGAATG	20	7.031155E-4	45.0	21
CGTTTTT	22330	0.0	44.445812	1
GATACCT	2400	0.0	43.6875	5
CGATGAA	560	0.0	43.392857	19
TGATACC	2420	0.0	43.326447	4
TACGGCT	2265	0.0	42.81457	7
CCGATGA	545	0.0	42.522938	18
ACGGCTG	2280	0.0	42.43421	8
CACCGGT	150	0.0	42.0	16
ATACCTG	2540	0.0	41.45669	6
CGGCTGT	2360	0.0	41.28178	9
ATGATGC	120	0.0	41.250004	3
ATGATAC	8060	0.0	41.008068	3
GAATGAT	8370	0.0	40.94086	1
GATGAAT	630	0.0	40.714287	20
>>END_MODULE