SRR2933427.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404432
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35431	8.76068164734739	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	10998	2.7193693871899356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	10812	2.6733789611108913	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	6801	1.681617676148277	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	2962	0.7323851722910155	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2775	0.6861474858567077	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	2477	0.6124638999881314	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	2248	0.5558412786327491	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	1751	0.4329528820667009	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	1188	0.29374530205325	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTACGGTAGTCGTATGCCG	911	0.22525418364521105	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGT	864	0.21363294694781818	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	834	0.20621513628990784	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	705	0.17431855046089328	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTAT	535	0.13228429006606796	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	522	0.12906990544764013	No Hit
GAATCTTTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	518	0.12808086402658542	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	503	0.12437195869763025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	502	0.12412469834236657	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	473	0.1169541480397199	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	471	0.11645962732919254	No Hit
GAATCTGTCTCTTATCCACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	451	0.11151442022391898	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	449	0.11101989951339163	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.027857E-4	45.000004	1
GGAATAG	20	7.027857E-4	45.000004	8
GGGCAAC	20	7.027857E-4	45.000004	7
GGCGCGA	20	7.027857E-4	45.000004	8
TTGCACG	20	7.027857E-4	45.000004	1
GGACATA	20	7.027857E-4	45.000004	8
CACCGGT	80	0.0	45.000004	16
CCGTTTG	20	7.027857E-4	45.000004	1
TACCGTA	20	7.027857E-4	45.000004	43
TCGCTTG	25	3.8863072E-5	45.0	1
CGGGCTA	25	3.8863072E-5	45.0	6
GCGATGC	25	3.8863072E-5	45.0	9
TGTTTAG	30	2.1619999E-6	44.999996	1
GACACGA	60	0.0	44.999996	25
CGACCAA	65	0.0	44.999996	29
CACGACC	60	0.0	44.999996	27
CGAGACA	65	0.0	44.999996	22
CACGTGA	60	0.0	44.999996	43
TCAAGCG	65	0.0	44.999996	17
AATCGTT	30	2.1619999E-6	44.999996	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content