##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933427.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404432
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.086976302567553	31.0	25.0	31.0	16.0	33.0
2	27.982120603710882	31.0	27.0	33.0	16.0	34.0
3	28.08257754084741	31.0	27.0	33.0	16.0	34.0
4	31.645839102741622	35.0	28.0	37.0	19.0	37.0
5	33.70216748427424	35.0	33.0	37.0	30.0	37.0
6	32.150601337184	35.0	32.0	37.0	17.0	37.0
7	32.67293883767852	35.0	32.0	37.0	27.0	37.0
8	32.4916994698738	35.0	32.0	37.0	23.0	37.0
9	33.426227400403526	35.0	32.0	39.0	27.0	39.0
10	32.52414744629505	35.0	28.0	39.0	17.0	39.0
11	33.15279948174229	37.0	31.0	39.0	17.0	39.0
12	33.791500178027455	37.0	31.0	39.0	25.0	39.0
13	33.891932389128456	37.0	32.0	39.0	25.0	39.0
14	34.26300094947977	37.0	32.0	40.0	23.0	41.0
15	34.54147545199193	37.0	32.0	40.0	25.0	41.0
16	34.635290975986074	37.0	32.0	40.0	25.0	41.0
17	32.762803141195555	36.0	29.0	40.0	18.0	41.0
18	33.29954602998774	36.0	30.0	39.0	23.0	39.0
19	32.958368773984255	36.0	31.0	37.0	23.0	39.0
20	32.23217994619615	34.0	30.0	35.0	24.0	39.0
21	32.77824455038177	35.0	32.0	37.0	25.0	39.0
22	32.81043290343	34.0	30.0	37.0	25.0	39.0
23	33.74960438343158	35.0	32.0	38.0	27.0	39.0
24	32.260963524152395	35.0	31.0	37.0	21.0	39.0
25	31.602956739328242	35.0	30.0	37.0	19.0	39.0
26	30.33939698144558	33.0	25.0	36.0	18.0	39.0
27	32.37990564544843	34.0	31.0	37.0	22.0	39.0
28	32.26590873125767	35.0	31.0	37.0	21.0	39.0
29	32.321915674328444	35.0	31.0	38.0	21.0	40.0
30	31.590860762748743	35.0	30.0	37.0	19.0	40.0
31	31.81712129603988	35.0	30.0	37.0	21.0	39.0
32	31.554295901412353	35.0	30.0	37.0	18.0	39.0
33	31.177367270641295	35.0	30.0	38.0	15.0	40.0
34	30.143500019780827	35.0	25.0	37.0	10.0	40.0
35	29.484820686790364	35.0	24.0	37.0	8.0	40.0
36	28.8090754440796	34.0	22.0	37.0	7.0	40.0
37	28.839080488190845	35.0	21.0	38.0	7.0	40.0
38	28.744963306563278	34.0	21.0	38.0	7.0	40.0
39	28.15417177671401	33.0	20.0	37.0	7.0	39.0
40	27.96763856470309	33.0	20.0	37.0	7.0	39.0
41	27.263161668710687	33.0	18.0	37.0	7.0	39.0
42	27.73338163152273	33.0	18.0	37.0	7.0	39.0
43	27.78831546465166	33.0	18.0	37.0	7.0	40.0
44	27.82250415397397	33.0	18.0	37.0	7.0	40.0
45	28.12674318550461	34.0	18.0	38.0	7.0	40.0
46	27.784203524943624	33.0	18.0	37.0	7.0	40.0
47	27.800782331764054	33.0	18.0	37.0	7.0	40.0
48	27.649684001265975	33.0	18.0	37.0	7.0	40.0
49	27.548512481702733	33.0	18.0	37.0	7.0	39.0
50	27.596216422043756	33.0	18.0	37.0	7.0	40.0
51	25.91526387625114	31.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	4.0
10	17.0
11	24.0
12	30.0
13	53.0
14	91.0
15	153.0
16	406.0
17	722.0
18	1351.0
19	2213.0
20	3406.0
21	5073.0
22	6973.0
23	9934.0
24	13955.0
25	19348.0
26	24023.0
27	25253.0
28	24264.0
29	24419.0
30	26116.0
31	29355.0
32	33068.0
33	36337.0
34	38491.0
35	40104.0
36	24872.0
37	11903.0
38	2429.0
39	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.495183368279463	1.9402520077540848	26.599279977845473	44.96528464612098
2	45.25927720852949	17.08841041262808	26.54142105471377	11.110891324128655
3	10.224462950508368	16.970961743877833	61.74585591644578	11.058719389168019
4	9.856539541876014	2.379880919412905	74.7821636270127	12.981415911698383
5	21.035674724057444	2.7164022629267714	61.9649285912094	14.282994421806386
6	11.192981762076196	14.389316374569766	64.20312932705622	10.214572536297819
7	40.78510108003323	1.1875914863314474	55.09257427701072	2.9347331566245995
8	39.85367132175496	11.83932033073545	43.28762313565692	5.019385211852673
9	36.70481069747201	2.445157653202516	43.33237725995965	17.517654389365827
10	25.13648771610555	14.20139850456937	48.19376310479883	12.46835067452625
11	21.56431736361119	12.832070657119122	53.38425248249396	12.219359496775725
12	15.658503778138227	11.083445424694387	55.628881987577635	17.629168809589746
13	15.727489417256797	12.387991059065554	62.16842386359141	9.716095660086244
14	13.694762036634096	16.59166435890335	54.420520631404045	15.293052973058513
15	9.020305020374252	12.890671361316612	64.02534913162164	14.063674486687502
16	11.07231870870752	13.476431142936265	52.87440162994026	22.57684851841595
17	12.236173200933655	14.663528108557186	58.452595244688844	14.647703445820312
18	11.658325750682438	12.862483680816553	57.184149622186176	18.29504094631483
19	11.803962099932745	15.725511334414685	54.37279938283815	18.097727182814417
20	12.31381295248645	16.030136092099536	60.68535625271987	10.970694702694148
21	13.744461368042094	19.92646477034458	53.839211536179135	12.48986232543419
22	10.877230288404478	12.647119911381887	58.281985599556904	18.193664200656723
23	11.847727182814417	19.665110574830873	55.13238319420818	13.354779048146536
24	13.415110574830875	14.79012541045219	52.91965027495351	18.87511373976342
25	11.043141986786406	21.367003600110774	51.387872374095025	16.20198203900779
26	11.15094750168137	13.476183882581003	56.81993511888278	18.55293349685485
27	12.739595284250505	17.0290679273648	52.99852632828262	17.23281046010207
28	9.25198797325632	15.459211931795705	58.31215136289908	16.9766487320489
29	15.08436523321597	13.631957906397119	54.78621869683903	16.49745816354789
30	12.703000751671482	19.918057918265617	54.123808205087634	13.255133124975274
31	15.544516754361673	14.297335522411677	51.34262372908177	18.815523994144876
32	18.994293231000515	20.12724017881869	48.36931795703604	12.509148633144756
33	16.386191003679233	15.25052419195316	47.28013609209954	21.08314871226807
34	16.77018633540373	18.158553230209282	50.756863947462115	14.31439648692487
35	14.49366024449104	25.056869881710647	42.719913359971514	17.7295565138268
36	18.200340230248845	18.135805277525023	44.2163330300273	19.447521462198836
37	17.763678442853188	23.79089686276061	43.359575899038646	15.085848795347548
38	21.40310361197927	20.139603196581874	38.38890097717292	20.068392214265934
39	22.035101080033233	18.44166633698619	44.18320014242197	15.340032440558609
40	20.196473078292517	22.96801440044309	41.55853147129802	15.276981049966373
41	19.76104759267318	21.24238438105788	41.93041104561459	17.066156980654352
42	17.127477548759742	23.130958974561857	43.35215808838074	16.38940538829766
43	23.03032400996954	18.23767654389366	39.92908573011037	18.802913716026424
44	19.23487755667207	20.18633540372671	41.43292321082407	19.14586382877715
45	16.09837995015231	19.00270008307948	40.55935237567749	24.339567591090717
46	22.690093761126718	20.753303398346322	39.09829093642442	17.458311904102544
47	15.609298967440758	18.29355738418325	48.534240613996914	17.56290303437908
48	17.440756418878824	21.57519681924279	40.140493333860825	20.843553428017568
49	16.598587648850735	18.628100644855007	45.28771214938482	19.485599556909445
50	19.90618942121296	16.87304664319342	41.7778514064169	21.442912529176724
51	17.061459033904338	17.676890058155635	40.54105510938798	24.720595798552043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	401.0
2	747.0
3	25236.0
4	49725.0
5	32457.0
6	15189.0
7	14449.0
8	13709.0
9	13330.0
10	12951.0
11	12315.0
12	11679.0
13	10787.5
14	9896.0
15	9042.5
16	8189.0
17	7454.0
18	6719.0
19	6112.0
20	5505.0
21	5112.0
22	4719.0
23	4446.0
24	4173.0
25	4118.5
26	4173.5
27	4283.0
28	4307.5
29	4332.0
30	4468.5
31	4605.0
32	5002.0
33	5399.0
34	5668.5
35	5938.0
36	6159.5
37	6381.0
38	7048.0
39	7715.0
40	8259.5
41	8804.0
42	9895.0
43	10986.0
44	12745.5
45	14505.0
46	35062.5
47	55620.0
48	44021.5
49	32423.0
50	28958.5
51	25494.0
52	20634.5
53	15775.0
54	14014.0
55	12253.0
56	11152.0
57	10051.0
58	9368.5
59	8686.0
60	7607.0
61	6528.0
62	5829.0
63	5130.0
64	4524.5
65	3919.0
66	3367.0
67	2815.0
68	2388.5
69	1962.0
70	1647.0
71	1332.0
72	1116.0
73	900.0
74	749.0
75	480.0
76	362.0
77	253.5
78	145.0
79	122.0
80	99.0
81	69.5
82	40.0
83	27.0
84	14.0
85	9.5
86	5.0
87	6.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	404432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.60646380118557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22334987796404	38.787460435007326
2	6.887934318102061	6.42044522923135
3	3.028316632096737	4.234173884770343
4	1.9048041574971906	3.5510474405896235
5	1.2932199831133466	3.0136205164971
6	0.9109013894438054	2.547233558013741
7	0.6273548848455356	2.0467154911735275
8	0.48944785006351366	1.8249146805242602
9	0.3321176663656139	1.393094699568289
>10	1.1788405689316626	8.77632288904194
>50	0.06590806083989895	2.122774907035172
>100	0.04754024060582873	4.299431184027112
>500	0.004862070061959756	1.4840037127345866
>1k	0.0032413800413065045	3.374131956965761
>5k	5.402300068844173E-4	1.7123700797943544
>10k+	0.0016206900206532523	14.41225933502553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35431	8.76068164734739	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	10998	2.7193693871899356	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	10812	2.6733789611108913	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	6801	1.681617676148277	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	2962	0.7323851722910155	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2775	0.6861474858567077	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	2477	0.6124638999881314	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	2248	0.5558412786327491	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	1751	0.4329528820667009	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	1188	0.29374530205325	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTACGGTAGTCGTATGCCG	911	0.22525418364521105	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGT	864	0.21363294694781818	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	834	0.20621513628990784	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	705	0.17431855046089328	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTAT	535	0.13228429006606796	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	522	0.12906990544764013	No Hit
GAATCTTTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	518	0.12808086402658542	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	503	0.12437195869763025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	502	0.12412469834236657	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	473	0.1169541480397199	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	471	0.11645962732919254	No Hit
GAATCTGTCTCTTATCCACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	451	0.11151442022391898	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	449	0.11101989951339163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25888159196107136	0.0
2	0.0	0.0	0.0	1.3574593503975947	0.0
3	0.0	0.0	0.0	1.6128792973849744	0.0
4	0.0	0.0	0.0	2.716896783637299	0.0
5	0.0	0.0	0.0	5.123976342129208	0.0
6	0.0	0.0	0.0	5.492888792182616	0.0
7	0.0	0.0	0.0	6.0873026862365	0.0
8	0.0	0.0	0.0	6.915377616014559	0.0
9	0.0	0.0	0.0	7.136181113265024	0.0
10	0.0	0.0	0.0	10.738764489456818	0.0
11	0.0	0.0	0.0	11.6449736914982	0.0
12	0.0	0.0	0.0	15.490366736558927	0.0
13	0.0	0.0	0.0	15.894390157059778	0.0
14	0.0	0.0	0.0	16.143628595165566	0.0
15	0.0	0.0	0.0	17.00211654864106	0.0
16	0.0	0.0	0.0	17.497873560944733	0.0
17	0.0	0.0	0.0	17.902638762511373	0.0
18	0.0	0.0	0.0	18.267100526170037	0.0
19	0.0	0.0	0.0	19.479170787672587	0.0
20	0.0	0.0	0.0	19.92423942714721	0.0
21	0.0	0.0	0.0	20.373016971950786	0.0
22	0.0	0.0	0.0	21.06040075958381	0.0
23	0.0	0.0	0.0	21.481237884242592	0.0
24	0.0	0.0	0.0	21.795505795782727	0.0
25	0.0	0.0	0.0	22.079855204335956	0.0
26	0.0	0.0	0.0	22.33206076670491	0.0
27	0.0	0.0	0.0	22.60478893856075	0.0
28	0.0	0.0	0.0	22.848834909205998	0.0
29	0.0	0.0	0.0	23.128239110653954	0.0
30	0.0	0.0	0.0	23.44374332397041	0.0
31	0.0	0.0	0.0	23.70188313486569	0.0
32	0.0	0.0	0.0	23.976836649918898	0.0
33	0.0	0.0	0.0	24.24659769751157	0.0
34	0.0	0.0	0.0	24.561360129762235	0.0
35	0.0	0.0	0.0	24.8842821537366	0.0
36	0.0	0.0	0.0	25.148108952802943	0.0
37	0.0	0.0	0.0	25.397594651263994	0.0
38	0.0	0.0	0.0	25.692823515448826	0.0
39	0.0	0.0	0.0	26.20391066977885	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	20	7.027857E-4	45.000004	1
GGAATAG	20	7.027857E-4	45.000004	8
GGGCAAC	20	7.027857E-4	45.000004	7
GGCGCGA	20	7.027857E-4	45.000004	8
TTGCACG	20	7.027857E-4	45.000004	1
GGACATA	20	7.027857E-4	45.000004	8
CACCGGT	80	0.0	45.000004	16
CCGTTTG	20	7.027857E-4	45.000004	1
TACCGTA	20	7.027857E-4	45.000004	43
TCGCTTG	25	3.8863072E-5	45.0	1
CGGGCTA	25	3.8863072E-5	45.0	6
GCGATGC	25	3.8863072E-5	45.0	9
TGTTTAG	30	2.1619999E-6	44.999996	1
GACACGA	60	0.0	44.999996	25
CGACCAA	65	0.0	44.999996	29
CACGACC	60	0.0	44.999996	27
CGAGACA	65	0.0	44.999996	22
CACGTGA	60	0.0	44.999996	43
TCAAGCG	65	0.0	44.999996	17
AATCGTT	30	2.1619999E-6	44.999996	22
>>END_MODULE