FastQCFastQC Report
Sat 14 Jan 2017
SRR2933426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533658
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178083.3369686203523603No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG175113.2813149994940582No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC157282.947205888415427No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC151012.8297149110478994No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT40200.7532914338396501No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC35450.6642831176521292No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT33830.6339265971839643No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA31230.5852062556918476No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT28300.5303021785488085No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC27190.5095023404502509TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGT12760.23910444516900337No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT8400.1574041802053No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT8090.1515952164120092No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATG7750.14522409483227086No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.10474873420805084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG453.8380676E-1045.01
CGACGAA351.210301E-745.019
TCCGATC207.0298865E-445.014
GCACGGG406.8030204E-945.03
CGTCGAT253.8879876E-545.040
CCGGTGA302.1633077E-644.99999618
CACCGGT650.044.99999616
TAACTCG302.1633077E-644.99999638
CGTTTTT140250.044.133691
CCGATGA5150.044.12621318
GATACCT19400.043.4922685
TGATACC19500.043.3846134
TACGGCT18450.042.926837
ACGGCTG18600.042.5806478
ATACCTG20100.041.8656736
CGGCTGT19100.041.583779
AGGCACG650.041.5384610
GTATGAT1750.041.1428571
CGATGAA5550.040.94594619
TACCTGT20750.040.662657
Produced by FastQC (version 0.11.5)