##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933426.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533658
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.283730404116493	31.0	28.0	31.0	25.0	34.0
2	28.339826255766802	31.0	26.0	33.0	16.0	34.0
3	28.32403711740478	31.0	25.0	33.0	16.0	34.0
4	33.002233640271484	35.0	32.0	37.0	28.0	37.0
5	34.21570556423777	35.0	33.0	37.0	30.0	37.0
6	33.46208620502269	35.0	32.0	37.0	28.0	37.0
7	33.65863905347619	35.0	33.0	37.0	29.0	37.0
8	33.37946962286708	35.0	33.0	37.0	28.0	37.0
9	34.71886114327903	37.0	34.0	39.0	28.0	39.0
10	34.661815244969624	37.0	33.0	39.0	28.0	39.0
11	34.892507935794086	37.0	34.0	39.0	27.0	39.0
12	34.96541230525917	37.0	34.0	39.0	27.0	39.0
13	34.99883445952277	37.0	34.0	39.0	27.0	39.0
14	35.785165780331226	38.0	34.0	40.0	27.0	41.0
15	36.06221962380401	38.0	34.0	40.0	27.0	41.0
16	36.10384928174973	38.0	34.0	40.0	30.0	41.0
17	35.32868241458012	37.0	33.0	40.0	27.0	41.0
18	35.118416663855875	37.0	33.0	39.0	27.0	40.0
19	34.43013877801888	37.0	33.0	38.0	27.0	40.0
20	33.60436646691327	35.0	32.0	37.0	26.0	40.0
21	34.13188409055987	35.0	33.0	38.0	27.0	40.0
22	34.67118828912899	35.0	33.0	39.0	29.0	40.0
23	34.95860270060601	35.0	33.0	39.0	30.0	40.0
24	34.511205678543185	35.0	33.0	38.0	27.0	40.0
25	33.580656525340196	35.0	33.0	38.0	24.0	40.0
26	33.51693406638709	35.0	32.0	38.0	24.0	40.0
27	34.25450756851767	35.0	33.0	38.0	27.0	40.0
28	33.93744308152412	35.0	33.0	39.0	25.0	40.0
29	34.009176288934114	35.0	33.0	39.0	24.0	40.0
30	33.302204782838444	35.0	33.0	39.0	21.0	40.0
31	33.19425549696622	35.0	33.0	38.0	21.0	40.0
32	32.573230420981226	35.0	31.0	38.0	18.0	40.0
33	31.863466864546208	35.0	30.0	39.0	14.0	40.0
34	31.42409745567386	35.0	30.0	39.0	10.0	40.0
35	30.967516649239776	35.0	27.0	39.0	8.0	40.0
36	30.312211191437214	35.0	24.0	39.0	7.0	40.0
37	30.296497007446717	35.0	23.0	39.0	7.0	40.0
38	29.968603487626908	35.0	23.0	39.0	7.0	40.0
39	29.703889007566644	35.0	22.0	39.0	7.0	40.0
40	29.414143515135162	35.0	22.0	39.0	7.0	40.0
41	28.96885271091223	35.0	21.0	39.0	7.0	40.0
42	29.214124026998565	35.0	21.0	39.0	7.0	40.0
43	29.085165030787508	35.0	20.0	39.0	7.0	40.0
44	29.235278024502584	35.0	20.0	39.0	7.0	40.0
45	29.343315381761354	35.0	20.0	39.0	7.0	40.0
46	29.07834418297861	35.0	20.0	39.0	7.0	40.0
47	29.016270720199078	35.0	20.0	39.0	7.0	40.0
48	28.872671261369643	35.0	20.0	38.0	7.0	40.0
49	29.077718313976366	35.0	20.0	39.0	7.0	40.0
50	28.962245483062187	35.0	20.0	39.0	7.0	40.0
51	27.674433813416083	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	26.0
10	35.0
11	44.0
12	39.0
13	67.0
14	84.0
15	147.0
16	280.0
17	566.0
18	1153.0
19	2095.0
20	3288.0
21	4865.0
22	7042.0
23	10260.0
24	14544.0
25	20675.0
26	26842.0
27	28487.0
28	26528.0
29	24673.0
30	25185.0
31	27965.0
32	32272.0
33	38045.0
34	42138.0
35	49257.0
36	52035.0
37	51611.0
38	37983.0
39	5414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.133156441016528	2.3732427884525293	28.705088277511067	38.78851249301987
2	38.38094809784543	19.28219946107807	29.724092958411568	12.612759482664929
3	11.700939553047082	19.48626273755851	56.05668799118536	12.756109718209041
4	10.475435578591533	2.6822421850698386	71.87037390988236	14.97194832645627
5	23.02485861731671	3.2389657795816795	56.882123007619114	16.8540525954825
6	12.69577144913034	16.884409115950668	60.43645930539784	9.983360129521154
7	48.43588965217424	1.3474922141146577	47.49671137694928	2.719906756761821
8	46.64223154154909	13.178477601759928	36.39671849761458	3.782572359076412
9	43.83781373089132	2.8218447020376347	35.49764081115621	17.842700755914837
10	29.371620026308985	15.895948341447145	41.549269382263546	13.183162249980324
11	25.092662341799432	13.825895985818635	47.454549542965715	13.626892129416218
12	18.371503847033118	12.988093498083042	49.85233988809312	18.788062766790716
13	18.14158131237609	14.028460174868549	56.875564500110556	10.954394012644803
14	14.820165724115444	20.76985634994697	48.39672599305173	16.013251932885854
15	11.158269903196429	14.15438351903279	59.74687908735557	14.94046749041521
16	14.129461190500283	16.185646987396424	47.46804132984046	22.216850492262836
17	14.624534814431714	16.836438318173812	53.25939084582261	15.279636021571868
18	14.578250490014206	15.519677396384951	51.305517766059914	18.596554347540934
19	14.554452477054594	17.862001506582867	48.07610866884784	19.5074373475147
20	16.32075224207264	17.842513369986023	53.91168126403052	11.92505312391082
21	15.987392674709271	22.940534949349583	48.63995292865469	12.432119447286464
22	13.796663780923362	13.960626468637216	52.541327966600335	19.70138178383909
23	14.595302609536445	21.58479775436703	50.55335064779316	13.26654898830337
24	16.270720199078813	16.853865209553685	47.683347762049856	19.192066829317653
25	13.74101016006506	25.30909308958172	45.488496377829996	15.461400372523226
26	13.52382986856751	14.780627293135304	51.40370799275941	20.291834845537778
27	16.209819772213667	18.223468963268612	46.810316719696885	18.75639454482084
28	10.950271522210855	18.225904980343216	53.18724726322851	17.636576234217422
29	17.035629560505043	15.148465871400784	49.389496643918015	18.426407924176157
30	14.144077292947918	20.254732431632245	49.372631910324586	16.228558365095246
31	18.214287052756635	15.311304243541743	47.576163010767196	18.898245692934427
32	22.180497622072565	20.604019802944958	43.613512774098766	13.601969800883712
33	16.59546001371665	19.691262943683032	40.35374715641853	23.359529886181786
34	18.76913678798032	18.632345059944758	45.14426842659531	17.454249725479613
35	16.16953179751826	21.213211457525233	41.16437868447583	21.452878060480682
36	19.735298636954752	21.424582785229493	42.39456730715177	16.445551270663984
37	19.904508130675453	21.398161369266457	38.807438471830274	19.889892028227816
38	19.117299843720133	25.190477796641296	38.0657649655772	17.626457394061365
39	23.65972214414475	20.78690846946921	35.234738353027595	20.318631033358443
40	18.046951418324095	23.89395455516454	40.46898950264027	17.590104523871094
41	21.263805658305508	22.94877993021748	33.741459886294216	22.045954525182797
42	17.752380738225604	21.032758808075585	43.49864519973466	17.716215253964148
43	23.060461943791715	20.682534507118792	35.10731592143283	21.149687627656665
44	20.746807880702622	20.846497194832644	35.70132931577902	22.705365608685714
45	17.06336267796979	20.149983697424194	37.764823163898974	25.021830460707044
46	24.586345562139048	22.61860592364398	33.98018955960559	18.81485895461138
47	15.369206495545837	21.341008660977632	44.41327591828475	18.87650892519179
48	19.166394957069883	23.48001903841037	34.30717800538922	23.04640799913053
49	17.514587994558312	19.506875189728255	42.44328764864389	20.535249167069548
50	19.896263149807556	19.649663267485916	38.00561408242732	22.448459500279206
51	18.513729767004335	19.867967874556363	36.00901701089462	25.60928534754468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	962.5
2	1790.0
3	19971.0
4	38152.0
5	27886.0
6	17620.0
7	16683.5
8	15747.0
9	15283.0
10	14819.0
11	14189.5
12	13560.0
13	12499.5
14	11439.0
15	10558.0
16	9677.0
17	8967.0
18	8257.0
19	7646.0
20	7035.0
21	6750.0
22	6465.0
23	6074.0
24	5683.0
25	5591.5
26	5799.0
27	6098.0
28	6278.0
29	6458.0
30	6994.0
31	7530.0
32	8164.0
33	8798.0
34	9594.5
35	10391.0
36	10854.5
37	11318.0
38	12040.5
39	12763.0
40	13583.5
41	14404.0
42	15607.0
43	16810.0
44	18358.5
45	19907.0
46	54112.5
47	88318.0
48	60964.5
49	33611.0
50	30388.5
51	27166.0
52	23950.0
53	20734.0
54	20008.0
55	19282.0
56	18333.5
57	17385.0
58	16253.5
59	15122.0
60	13709.0
61	12296.0
62	10860.0
63	9424.0
64	8378.5
65	7333.0
66	6012.0
67	4691.0
68	3728.5
69	2766.0
70	2328.0
71	1890.0
72	1614.5
73	1339.0
74	1146.5
75	715.0
76	476.0
77	362.0
78	248.0
79	194.0
80	140.0
81	110.5
82	81.0
83	53.5
84	26.0
85	19.5
86	13.0
87	8.5
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.882152918181255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.29556446555098	36.147752857361624
2	5.2378144380179865	4.492175193762899
3	2.0904058423547554	2.6892330897874834
4	1.3845729965091864	2.3749388385076546
5	0.9975129616710477	2.1387751680122866
6	0.8751136163842094	2.2516053551142163
7	0.718530899811687	2.1568506345514193
8	0.6687463691562179	2.294182725250834
9	0.5567342333832644	2.1486566277654795
>10	3.0448392851652994	20.2070638139265
>50	0.07615088919472916	2.2902265285089625
>100	0.04693019915472049	3.6194046704282647
>500	0.002213688639373608	0.6633550104110552
>1k	0.0030991640951230507	3.967219890540758
>5k	0.0	0.0
>10k+	0.0017709509114988864	12.558559596070543
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17808	3.3369686203523603	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	17511	3.2813149994940582	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	15728	2.947205888415427	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	15101	2.8297149110478994	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	4020	0.7532914338396501	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC	3545	0.6642831176521292	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT	3383	0.6339265971839643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	3123	0.5852062556918476	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	2830	0.5303021785488085	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	2719	0.5095023404502509	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGT	1276	0.23910444516900337	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	840	0.1574041802053	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTAT	809	0.1515952164120092	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATG	775	0.14522409483227086	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.10474873420805084	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.621577864475001E-4	0.0	0.0	0.08694707097054669	0.0
2	5.621577864475001E-4	0.0	0.0	0.7060701797780601	0.0
3	5.621577864475001E-4	0.0	0.0	0.7999505301147927	0.0
4	5.621577864475001E-4	0.0	0.0	1.704837180366452	0.0
5	5.621577864475001E-4	0.0	0.0	5.080032530197243	0.0
6	5.621577864475001E-4	0.0	0.0	5.329255815522301	0.0
7	5.621577864475001E-4	0.0	0.0	6.000659598469432	0.0
8	5.621577864475001E-4	0.0	0.0	6.833215280198179	0.0
9	5.621577864475001E-4	0.0	0.0	6.943210820413073	0.0
10	5.621577864475001E-4	0.0	0.0	10.836340877490828	0.0
11	5.621577864475001E-4	0.0	0.0	11.058205817208774	0.0
12	5.621577864475001E-4	0.0	0.0	14.656765194188038	0.0
13	5.621577864475001E-4	0.0	0.0	14.930910808045603	0.0
14	5.621577864475001E-4	0.0	0.0	15.146966783970258	0.0
15	5.621577864475001E-4	0.0	0.0	15.916748179545701	0.0
16	5.621577864475001E-4	0.0	0.0	16.281775968878947	0.0
17	5.621577864475001E-4	0.0	0.0	16.467475424335436	0.0
18	5.621577864475001E-4	0.0	0.0	16.598270802648887	0.0
19	5.621577864475001E-4	0.0	0.0	17.48816657859528	0.0
20	5.621577864475001E-4	0.0	0.0	17.684547031994274	0.0
21	5.621577864475001E-4	0.0	0.0	17.838578265480887	0.0
22	5.621577864475001E-4	0.0	0.0	18.14888936359991	0.0
23	5.621577864475001E-4	0.0	0.0	18.301796281513628	0.0
24	5.621577864475001E-4	0.0	0.0	18.432404273898264	0.0
25	5.621577864475001E-4	0.0	0.0	18.538839481465658	0.0
26	5.621577864475001E-4	0.0	0.0	18.688186066731877	0.0
27	5.621577864475001E-4	0.0	0.0	18.88100618748337	0.0
28	5.621577864475001E-4	0.0	0.0	18.996998077420372	0.0
29	5.621577864475001E-4	0.0	0.0	19.136600594388167	0.0
30	5.621577864475001E-4	0.0	0.0	19.293630002735835	0.0
31	5.621577864475001E-4	0.0	0.0	19.428360485554418	0.0
32	5.621577864475001E-4	0.0	0.0	19.583703420542744	0.0
33	5.621577864475001E-4	0.0	0.0	19.725741954585146	0.0
34	5.621577864475001E-4	0.0	0.0	19.924558425058745	0.0
35	5.621577864475001E-4	0.0	0.0	20.109883108657606	0.0
36	5.621577864475001E-4	0.0	0.0	20.268598990364616	0.0
37	5.621577864475001E-4	0.0	0.0	20.434435537366628	0.0
38	5.621577864475001E-4	0.0	0.0	20.64655640878615	0.0
39	5.621577864475001E-4	0.0	0.0	21.011022040332946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	45	3.8380676E-10	45.0	1
CGACGAA	35	1.210301E-7	45.0	19
TCCGATC	20	7.0298865E-4	45.0	14
GCACGGG	40	6.8030204E-9	45.0	3
CGTCGAT	25	3.8879876E-5	45.0	40
CCGGTGA	30	2.1633077E-6	44.999996	18
CACCGGT	65	0.0	44.999996	16
TAACTCG	30	2.1633077E-6	44.999996	38
CGTTTTT	14025	0.0	44.13369	1
CCGATGA	515	0.0	44.126213	18
GATACCT	1940	0.0	43.492268	5
TGATACC	1950	0.0	43.384613	4
TACGGCT	1845	0.0	42.92683	7
ACGGCTG	1860	0.0	42.580647	8
ATACCTG	2010	0.0	41.865673	6
CGGCTGT	1910	0.0	41.58377	9
AGGCACG	65	0.0	41.53846	10
GTATGAT	175	0.0	41.142857	1
CGATGAA	555	0.0	40.945946	19
TACCTGT	2075	0.0	40.66265	7
>>END_MODULE