SRR2933425.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350486
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24722	7.05363409665436	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	8793	2.5088020634205077	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	7103	2.0266144724753627	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	5281	1.5067648921782897	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	1975	0.5635032497731721	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	1788	0.5101487648579401	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT	1757	0.50130390372226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	1410	0.4022985226228722	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC	1375	0.39231238908258814	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	832	0.23738466015760973	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCTGACTGTCGTATGCCG	696	0.19858139840107739	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	544	0.15521304702612945	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGT	522	0.14893604880080802	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	461	0.13153164463059866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	400	0.1141272404603893	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	376	0.10727960603276593	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	369	0.10528237932470912	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	354	0.10100260780744452	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCTGCCTCCTATACACATCTGACGCTCTGACTGTCGTATGC	353	0.10071728970629354	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCG	75	0.0	45.000004	11
ACCGCTG	20	7.026568E-4	45.000004	17
CTCCCGA	20	7.026568E-4	45.000004	44
ACGGGAG	30	2.1611686E-6	45.000004	5
TCCCGAG	20	7.026568E-4	45.000004	45
CACCGCT	20	7.026568E-4	45.000004	16
TATCGCG	20	7.026568E-4	45.000004	1
ATCGCGG	20	7.026568E-4	45.000004	2
TAACCGC	25	3.885239E-5	45.0	22
GTAACCG	25	3.885239E-5	45.0	21
CCGATGA	105	0.0	44.999996	18
CGTTTTT	10780	0.0	44.45733	1
TGATACC	1025	0.0	43.02439	4
ACGGCGA	1145	0.0	42.44541	8
GATACCT	1035	0.0	42.391304	5
CACCGGT	85	0.0	42.35294	16
CTACGGC	80	0.0	42.187504	6
GAATGAT	3205	0.0	42.05148	1
TACGGCT	835	0.0	42.035927	7
ATACGGC	2005	0.0	41.857853	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content