##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933425.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	350486
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.177690407034802	31.0	26.0	31.0	16.0	33.0
2	28.238035185428235	31.0	28.0	33.0	16.0	34.0
3	28.41083524020931	31.0	28.0	33.0	16.0	34.0
4	31.724317091124895	35.0	28.0	37.0	19.0	37.0
5	33.61267497132553	35.0	33.0	37.0	28.0	37.0
6	32.28863635066736	35.0	32.0	37.0	17.0	37.0
7	32.250828849083845	35.0	32.0	35.0	26.0	37.0
8	32.599832803592726	35.0	32.0	35.0	25.0	37.0
9	33.04650685048761	35.0	32.0	37.0	25.0	39.0
10	31.86035961493469	35.0	28.0	39.0	15.0	39.0
11	32.473456857049925	35.0	29.0	39.0	17.0	39.0
12	33.27708096757075	35.0	31.0	39.0	23.0	39.0
13	33.294941880702794	35.0	31.0	39.0	23.0	39.0
14	33.53838099096683	36.0	31.0	40.0	20.0	41.0
15	34.06843069338005	37.0	32.0	40.0	24.0	41.0
16	34.252244026865554	37.0	32.0	40.0	25.0	41.0
17	32.311764235946654	35.0	27.0	39.0	18.0	41.0
18	32.96660066307927	36.0	30.0	39.0	23.0	39.0
19	32.740720028760066	36.0	30.0	37.0	21.0	39.0
20	32.20767733946577	34.0	30.0	36.0	23.0	39.0
21	32.65002025758518	35.0	31.0	37.0	24.0	39.0
22	32.894483659832346	34.0	30.0	37.0	25.0	39.0
23	33.879184903248635	35.0	32.0	38.0	27.0	40.0
24	32.26756560889736	35.0	31.0	37.0	21.0	39.0
25	31.524939655221605	35.0	30.0	37.0	18.0	39.0
26	30.62889245219495	33.0	25.0	37.0	19.0	39.0
27	32.43178900155784	34.0	30.0	37.0	22.0	39.0
28	32.38139326535154	35.0	31.0	37.0	21.0	39.0
29	32.598531753051475	35.0	31.0	38.0	22.0	40.0
30	31.639725980495655	35.0	30.0	37.0	18.0	40.0
31	31.88218644967274	35.0	30.0	37.0	20.0	40.0
32	31.694726750854528	35.0	30.0	37.0	18.0	40.0
33	31.5046107405146	35.0	30.0	38.0	15.0	40.0
34	30.74032914296149	35.0	29.0	38.0	10.0	40.0
35	30.230927911528564	35.0	27.0	38.0	9.0	40.0
36	29.517980746734533	34.0	24.0	38.0	8.0	40.0
37	29.680049987731323	35.0	24.0	38.0	7.0	40.0
38	29.48053274595847	35.0	23.0	38.0	8.0	40.0
39	29.109239741387672	34.0	23.0	38.0	7.0	40.0
40	28.732448657007698	34.0	22.0	37.0	7.0	40.0
41	28.306831085977755	33.0	21.0	37.0	7.0	40.0
42	28.81312805647016	34.0	22.0	38.0	7.0	40.0
43	28.802006356887293	34.0	22.0	38.0	7.0	40.0
44	28.87950160634091	34.0	21.0	38.0	7.0	40.0
45	29.058247690349972	35.0	22.0	38.0	7.0	40.0
46	28.756212801652563	34.0	21.0	38.0	7.0	40.0
47	28.81106805977985	34.0	22.0	38.0	7.0	40.0
48	28.628581455464698	34.0	22.0	37.0	7.0	40.0
49	28.49064156628225	34.0	20.0	37.0	7.0	40.0
50	28.582847817031208	34.0	20.0	38.0	7.0	40.0
51	26.873649732086303	31.0	18.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	15.0
10	20.0
11	15.0
12	26.0
13	33.0
14	76.0
15	161.0
16	371.0
17	673.0
18	1211.0
19	1885.0
20	2913.0
21	4183.0
22	5837.0
23	7953.0
24	10938.0
25	15050.0
26	18788.0
27	20229.0
28	19907.0
29	20572.0
30	22757.0
31	25590.0
32	29333.0
33	31905.0
34	34714.0
35	35963.0
36	24415.0
37	12357.0
38	2545.0
39	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.465251108460823	2.4274864045924804	30.90565671667342	40.20160577027328
2	41.44302482838116	15.029416296228664	30.62775688615237	12.899801989237803
3	12.171099558898216	14.904446967924539	60.08028851366388	12.844164959513362
4	11.254087181798987	2.695970737775546	70.656174569027	15.393767511398456
5	20.290967399553764	3.232654086040527	59.982139086867946	16.494239427537764
6	12.97912042135777	13.03703999589142	62.71177736057931	11.272062222171499
7	48.20620509806383	1.4049063300673923	47.45410658342987	2.9347819884389104
8	47.96111684917514	10.194415754124272	37.21860502273985	4.625862373960729
9	44.22116717928819	2.7310648642171156	37.49393699035054	15.553830966144155
10	27.18796185867624	16.08338136188036	42.901000325262636	13.827656454180767
11	22.82059768435829	14.443943552666868	49.490707189445516	13.244751573529328
12	18.063774301969264	13.282128244780106	51.541003064316406	17.113094388934222
13	17.31795278556062	14.439093144947302	57.20257014545517	11.04038392403691
14	14.332384175116838	18.007851954143675	51.013449895288254	16.64631397545123
15	11.041810514542663	14.642524951067944	59.4120735207683	14.903591013621087
16	13.886146664916716	14.746095421785748	49.36431127063563	22.003446642661903
17	14.316121043351233	15.820603390720315	54.094029433415315	15.76924613251314
18	14.024240625873787	14.424256603687452	53.11167921115252	18.439823559286246
19	14.627117773605795	17.069726037559274	49.60597570231051	18.697180486524427
20	15.321582031807262	17.390423583252968	55.11404164503004	12.173952739909726
21	15.994076796220105	20.846196424393558	49.71896167036629	13.440765109020047
22	13.476715189765068	14.389447795347031	53.07401722180059	19.059819793087314
23	14.017678309547316	20.506097247821597	51.06109801818047	14.41512642445062
24	15.218582197291761	16.192658194621185	49.57972643700462	19.00903317108244
25	13.460452057999465	21.725832130242008	47.59277118058924	17.220944631169292
26	13.173422048241584	15.308172081053167	52.32819570539194	19.190210165313307
27	14.190010442642503	17.44948443019122	49.30068533407896	19.059819793087314
28	10.906284416495952	17.100255074382428	54.753114247074066	17.240346262047556
29	16.222616595242034	14.74894860279726	50.663364585176005	18.365070216784694
30	13.863891853026939	18.98820494969842	51.226582516848026	15.921320680426607
31	16.886551816620347	15.561819872976383	49.07214553505703	18.479482775346234
32	19.515187482524265	19.863560884029603	46.56106092682732	14.060190706618808
33	15.734722642273871	18.289746238080838	43.712445004936	22.263086114709292
34	17.216664859652024	18.39131948209058	47.36765519878112	17.02436045947627
35	15.009158711046947	20.461872942143195	43.090166226325735	21.438802120484127
36	18.125117693716724	19.9788293968946	44.77154579640842	17.12450711298026
37	18.86780071101271	20.44618044657989	41.22846561631563	19.45755322609177
38	18.423845745621794	23.715069931466594	40.9999258172937	16.86115850561791
39	21.298710932819002	20.177981431497976	38.60810417534509	19.91520346033793
40	17.24434071546367	22.325285460760202	42.735801144696225	17.694572679079908
41	19.853004114287018	20.96745661738272	37.826617896292575	21.352921372037684
42	17.55819062672974	19.63102663159156	45.05229880794097	17.758483933737722
43	21.302990704336263	19.43387182369624	38.731646913143464	20.531490558824032
44	19.609342455904088	19.582237236294745	38.886574642068446	21.921845665732725
45	16.560147908903637	19.178226805064966	40.518023544449704	23.743601741581692
46	22.053377310363324	21.461627568576205	37.57553796727972	18.90945715378075
47	14.815142402264284	20.124626946582744	45.93221983189058	19.128010819262396
48	18.249231067717396	21.62140570522075	38.02320206798559	22.106161159076255
49	17.006385419103758	18.53369321456492	44.026294916202076	20.43362645012925
50	19.05325747676084	18.57820283834447	40.03726254401032	22.331277140884374
51	17.887447715457963	18.640972820597685	38.582140228140354	24.889439235803998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	380.0
2	660.0
3	18421.5
4	36183.0
5	23947.5
6	11712.0
7	10995.0
8	10278.0
9	10010.5
10	9743.0
11	9058.5
12	8374.0
13	7730.5
14	7087.0
15	6486.5
16	5886.0
17	5413.5
18	4941.0
19	4526.0
20	4111.0
21	3906.0
22	3701.0
23	3481.0
24	3261.0
25	3133.5
26	3232.5
27	3459.0
28	3624.5
29	3790.0
30	4146.0
31	4502.0
32	4913.5
33	5325.0
34	5804.5
35	6284.0
36	6716.0
37	7148.0
38	7547.0
39	7946.0
40	8652.0
41	9358.0
42	10471.0
43	11584.0
44	12512.5
45	13441.0
46	28887.0
47	44333.0
48	36039.0
49	27745.0
50	25146.0
51	22547.0
52	19244.5
53	15942.0
54	14347.5
55	12753.0
56	11677.5
57	10602.0
58	9822.5
59	9043.0
60	8065.5
61	7088.0
62	6468.0
63	5848.0
64	5108.5
65	4369.0
66	3625.5
67	2882.0
68	2370.5
69	1859.0
70	1607.0
71	1355.0
72	1148.0
73	941.0
74	779.0
75	471.0
76	325.0
77	251.0
78	177.0
79	141.5
80	106.0
81	75.5
82	45.0
83	32.0
84	19.0
85	13.0
86	7.0
87	4.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	350486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.183018014938845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80198649787219	36.96060629591143
2	6.701571978514837	6.055944948672904
3	2.902568094968667	3.9344035957366805
4	1.8921895171875807	3.419793321710595
5	1.3839756548313211	3.1266098472240182
6	1.084751335899538	2.9407403491006687
7	0.8391795113997369	2.6541664086939325
8	0.6753478767974114	2.4411404226950513
9	0.5476944696641075	2.227184018056417
>10	2.049930299666158	13.813539089583953
>50	0.07004105951400892	2.1635866434650266
>100	0.04241018282499623	3.771759807468352
>500	0.002570314110605832	0.753132548731653
>1k	0.00321289263825729	2.411243568703307
>5k	0.0019277355829543739	6.148453347914502
>10k+	6.42578527651458E-4	7.1776957863315065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24722	7.05363409665436	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	8793	2.5088020634205077	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	7103	2.0266144724753627	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	5281	1.5067648921782897	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	1975	0.5635032497731721	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	1788	0.5101487648579401	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT	1757	0.50130390372226	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	1410	0.4022985226228722	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC	1375	0.39231238908258814	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	832	0.23738466015760973	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCTGACTGTCGTATGCCG	696	0.19858139840107739	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	544	0.15521304702612945	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGT	522	0.14893604880080802	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	461	0.13153164463059866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	400	0.1141272404603893	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTT	376	0.10727960603276593	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	369	0.10528237932470912	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	354	0.10100260780744452	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACGGCTGCCTCCTATACACATCTGACGCTCTGACTGTCGTATGC	353	0.10071728970629354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18517144764698162	0.0
2	0.0	0.0	0.0	1.0394138424929955	0.0
3	0.0	0.0	0.0	1.2428456486136394	0.0
4	0.0	0.0	0.0	2.240603048338593	0.0
5	0.0	0.0	0.0	4.305735464469337	0.0
6	0.0	0.0	0.0	4.662097772806902	0.0
7	0.0	0.0	0.0	5.159121905011898	0.0
8	0.0	0.0	0.0	5.894957287880258	0.0
9	0.0	0.0	0.0	6.067574739076597	0.0
10	0.0	0.0	0.0	9.444314466198364	0.0
11	0.0	0.0	0.0	10.1878534377978	0.0
12	0.0	0.0	0.0	13.130053696866637	0.0
13	0.0	0.0	0.0	13.460452057999463	0.0
14	0.0	0.0	0.0	13.637634598814218	0.0
15	0.0	0.0	0.0	14.267902284256717	0.0
16	0.0	0.0	0.0	14.642239632966795	0.0
17	0.0	0.0	0.0	15.013723800665362	0.0
18	0.0	0.0	0.0	15.337274527370566	0.0
19	0.0	0.0	0.0	16.216054278915564	0.0
20	0.0	0.0	0.0	16.588679719018735	0.0
21	0.0	0.0	0.0	16.98898101493355	0.0
22	0.0	0.0	0.0	17.592428798867857	0.0
23	0.0	0.0	0.0	17.95050301581233	0.0
24	0.0	0.0	0.0	18.239815570379417	0.0
25	0.0	0.0	0.0	18.488898272684217	0.0
26	0.0	0.0	0.0	18.725712296639525	0.0
27	0.0	0.0	0.0	18.992199403114533	0.0
28	0.0	0.0	0.0	19.204761388472008	0.0
29	0.0	0.0	0.0	19.47267508545277	0.0
30	0.0	0.0	0.0	19.75656659609799	0.0
31	0.0	0.0	0.0	20.000513572582072	0.0
32	0.0	0.0	0.0	20.236186324132774	0.0
33	0.0	0.0	0.0	20.48327179972952	0.0
34	0.0	0.0	0.0	20.749758906204526	0.0
35	0.0	0.0	0.0	21.04706036760384	0.0
36	0.0	0.0	0.0	21.284730345862602	0.0
37	0.0	0.0	0.0	21.525538823234022	0.0
38	0.0	0.0	0.0	21.836820871589737	0.0
39	0.0	0.0	0.0	22.375216128461624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCG	75	0.0	45.000004	11
ACCGCTG	20	7.026568E-4	45.000004	17
CTCCCGA	20	7.026568E-4	45.000004	44
ACGGGAG	30	2.1611686E-6	45.000004	5
TCCCGAG	20	7.026568E-4	45.000004	45
CACCGCT	20	7.026568E-4	45.000004	16
TATCGCG	20	7.026568E-4	45.000004	1
ATCGCGG	20	7.026568E-4	45.000004	2
TAACCGC	25	3.885239E-5	45.0	22
GTAACCG	25	3.885239E-5	45.0	21
CCGATGA	105	0.0	44.999996	18
CGTTTTT	10780	0.0	44.45733	1
TGATACC	1025	0.0	43.02439	4
ACGGCGA	1145	0.0	42.44541	8
GATACCT	1035	0.0	42.391304	5
CACCGGT	85	0.0	42.35294	16
CTACGGC	80	0.0	42.187504	6
GAATGAT	3205	0.0	42.05148	1
TACGGCT	835	0.0	42.035927	7
ATACGGC	2005	0.0	41.857853	6
>>END_MODULE