##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933424.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1024875
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.418242224661544	31.0	28.0	33.0	25.0	34.0
2	28.573576289791436	31.0	27.0	33.0	16.0	34.0
3	28.53828905964142	31.0	27.0	33.0	16.0	34.0
4	33.179136967922915	35.0	32.0	37.0	28.0	37.0
5	34.289923161361145	35.0	33.0	37.0	30.0	37.0
6	33.720653738260765	35.0	33.0	37.0	28.0	37.0
7	33.85141114770094	35.0	33.0	37.0	30.0	37.0
8	33.610532016099526	35.0	33.0	37.0	28.0	37.0
9	34.922111477009395	37.0	34.0	39.0	28.0	39.0
10	34.85132040492743	37.0	33.0	39.0	28.0	39.0
11	35.31265081107452	37.0	34.0	39.0	30.0	39.0
12	35.45538821807538	37.0	34.0	39.0	30.0	39.0
13	35.645602634467615	37.0	34.0	39.0	30.0	39.0
14	36.46097231369679	38.0	35.0	40.0	30.0	41.0
15	36.71938236370289	38.0	35.0	41.0	30.0	41.0
16	36.64788096109282	38.0	34.0	41.0	31.0	41.0
17	35.88199829247469	38.0	34.0	40.0	27.0	41.0
18	35.58972264910355	38.0	34.0	39.0	28.0	40.0
19	34.90685498231492	37.0	34.0	38.0	27.0	40.0
20	33.865324063910236	35.0	33.0	37.0	27.0	40.0
21	34.26253152823515	35.0	33.0	38.0	28.0	40.0
22	34.662088547383824	35.0	33.0	38.0	29.0	40.0
23	34.93371289181608	35.0	33.0	39.0	30.0	40.0
24	34.36290572020978	35.0	33.0	38.0	27.0	40.0
25	33.42678570557385	35.0	33.0	38.0	24.0	40.0
26	33.32146066593487	35.0	32.0	38.0	24.0	40.0
27	34.15557019148677	35.0	33.0	38.0	25.0	40.0
28	33.80405025003049	35.0	33.0	38.0	24.0	40.0
29	33.734862300280525	35.0	33.0	39.0	23.0	40.0
30	32.95102134406635	35.0	32.0	38.0	21.0	40.0
31	32.80519770703744	35.0	33.0	38.0	20.0	40.0
32	31.839516038541287	35.0	31.0	38.0	15.0	40.0
33	30.755331137943653	35.0	25.0	39.0	9.0	40.0
34	29.7385788510794	35.0	21.0	39.0	7.0	40.0
35	29.13628588852299	35.0	18.0	39.0	7.0	40.0
36	28.30905915355531	35.0	15.0	39.0	7.0	40.0
37	28.315304793267472	35.0	15.0	39.0	7.0	40.0
38	28.15563166239785	35.0	15.0	39.0	7.0	40.0
39	27.750635931211125	35.0	15.0	39.0	7.0	40.0
40	27.600495670203685	34.0	15.0	39.0	7.0	40.0
41	27.049510428100987	33.0	12.0	38.0	7.0	40.0
42	27.35926917916819	34.0	12.0	39.0	7.0	40.0
43	27.194455909257226	34.0	12.0	38.0	7.0	40.0
44	27.361909501158678	34.0	11.0	39.0	7.0	40.0
45	27.555423832174654	35.0	10.0	39.0	7.0	40.0
46	27.29825588486401	34.0	10.0	39.0	7.0	40.0
47	27.212907915599462	34.0	10.0	39.0	7.0	40.0
48	26.982753994389558	34.0	10.0	38.0	7.0	40.0
49	27.16955140870838	34.0	10.0	39.0	7.0	40.0
50	27.062098304671302	34.0	10.0	39.0	7.0	40.0
51	25.88242761312355	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	29.0
10	45.0
11	48.0
12	86.0
13	133.0
14	174.0
15	297.0
16	576.0
17	1245.0
18	2621.0
19	4836.0
20	8173.0
21	12307.0
22	18296.0
23	27035.0
24	39652.0
25	57026.0
26	69529.0
27	63747.0
28	51186.0
29	42277.0
30	41068.0
31	44598.0
32	50994.0
33	59205.0
34	69650.0
35	82646.0
36	91877.0
37	97426.0
38	77005.0
39	11076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.690645200634222	2.1523600439077937	28.584656665447007	43.57233809001097
2	43.42412489327967	16.85912916209294	27.886815465300646	11.829930479326748
3	9.60126844737163	17.04256616660568	61.091059885351875	12.265105500670813
4	8.556555677521649	2.198316867910721	75.82573484571289	13.419392608854738
5	19.13257714355409	2.8511769728015612	63.207415538480305	14.808830345164045
6	10.692328332723502	13.73650445176241	66.70579338943774	8.86537382607635
7	45.72899134040737	0.7865349432857666	51.322161239175514	2.1623124771313575
8	44.414782290523235	10.070106110501282	42.3808757165508	3.134235882424686
9	41.7238199780461	2.1046469081595314	41.107939992682034	15.063593121112332
10	24.667496036101962	11.740651298938895	50.595731186730085	12.996121478229053
11	19.744651786803267	14.978997438712039	51.593413830954994	13.6829369435297
12	13.257909501158677	12.771118429076717	56.74106598365655	17.229906086108063
13	14.099865837297232	13.930186608122941	61.87417977802171	10.095767776558116
14	13.29488962068545	19.730015855592146	53.29088913282107	13.68420539090133
15	10.16845956824003	14.878107086230028	62.15294548115624	12.800487864373705
16	10.930698865715332	16.335162824734724	52.841468471764856	19.892669837785096
17	11.824856689840225	17.167752164898157	56.848688864495664	14.158702280765947
18	12.055226247103306	16.366483717526528	54.999390169532866	16.578899865837297
19	12.414391999024271	18.20397609464569	52.264910354921334	17.11672155140871
20	14.302134406634956	16.869667032564948	57.71923405293329	11.108964507866812
21	14.053713867544824	20.663397975362848	53.87798512013661	11.404903036955726
22	12.554116355653127	13.44115135992194	55.935504329796316	18.069227954628612
23	13.167166727649713	19.763190633004022	54.683644346871574	12.385998292474692
24	14.90825710452494	16.09474326137334	52.18402244176119	16.81297719234053
25	11.352603976094645	24.16616660568362	50.81341627027687	13.667813147944871
26	12.27076472740578	15.04817660690328	55.73382119770704	16.9472374679839
27	15.70454933528479	17.290498841322112	50.89479204781071	16.11015977558239
28	11.44324917672887	16.692864983534577	56.050933040614716	15.812952799121843
29	14.748335162824736	13.969020612269789	55.12535675082327	16.157287474082203
30	11.844273691913648	18.516209293816317	52.49869496280034	17.14082205146969
31	14.600219538968167	14.77282595438468	49.5452616172704	21.08169288937675
32	15.465007927796073	18.487230150018295	51.61868520551287	14.429076716672764
33	14.324283449201122	16.23505305525064	47.711184290767164	21.72947920478107
34	20.33584583485791	18.37043541895353	45.151750213440664	16.141968532747896
35	17.792901573362606	19.37202097816807	41.1731186730089	21.66195877546042
36	16.54484693255275	19.111794121234297	43.448615684839616	20.89474326137334
37	16.426881326991097	21.33177216733748	43.287230150018296	18.954116355653127
38	17.979168191242834	21.728601048908402	39.39904866447127	20.893182095377487
39	22.106988657153313	21.02334431028174	40.11337968044884	16.75628735211611
40	20.31564824978656	21.78519331625808	40.94099280400049	16.958165629954873
41	19.960190267105745	24.21348944993292	38.431418465666546	17.39490181729479
42	19.821831930723256	22.48742529576778	40.33428466886206	17.356458104646908
43	19.79353579704842	21.113599219417	39.340992804000486	19.75187217953409
44	19.843005244542017	22.149042566166603	35.71333089401146	22.294621295279914
45	17.66069032808879	21.474423710208562	36.23505305525064	24.629832906452005
46	22.361165995853153	23.3376753262593	35.61605073789487	18.685107939992683
47	16.428052201487986	20.85337236248323	43.45310403707769	19.265471398951092
48	20.071716062934506	23.081644102939382	33.88335162824735	22.963288205878765
49	17.81144041956336	21.095548237589952	40.184705451884376	20.90830589096231
50	18.690084156604463	20.28344920112209	38.64461519697524	22.381851445298206
51	18.39746310525674	20.63285766556897	35.52257592389316	25.447103305281132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	1176.5
2	2293.0
3	30012.5
4	57732.0
5	45610.0
6	33488.0
7	32700.5
8	31913.0
9	32157.5
10	32402.0
11	32059.5
12	31717.0
13	30951.5
14	30186.0
15	29090.0
16	27994.0
17	26860.0
18	25726.0
19	24308.5
20	22891.0
21	21589.0
22	20287.0
23	19139.5
24	17992.0
25	17087.0
26	15722.0
27	15262.0
28	15390.0
29	15518.0
30	15858.0
31	16198.0
32	17280.0
33	18362.0
34	19681.5
35	21001.0
36	22819.0
37	24637.0
38	25650.5
39	26664.0
40	29534.0
41	32404.0
42	34044.5
43	35685.0
44	39913.0
45	44141.0
46	100998.0
47	157855.0
48	110269.0
49	62683.0
50	57071.5
51	51460.0
52	44242.0
53	37024.0
54	33902.5
55	30781.0
56	27812.5
57	24844.0
58	22198.0
59	19552.0
60	17093.0
61	14634.0
62	12224.5
63	9815.0
64	8056.5
65	6298.0
66	4997.0
67	3696.0
68	2995.5
69	2295.0
70	1782.5
71	1270.0
72	1068.0
73	866.0
74	674.5
75	384.5
76	286.0
77	219.0
78	152.0
79	118.5
80	85.0
81	61.0
82	37.0
83	25.0
84	13.0
85	9.5
86	6.0
87	4.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1024875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46389469309547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.32540796904279	42.04924613008993
2	6.087173675360651	6.143649826639655
3	3.0531621237641433	4.622233556837442
4	1.9871215336130912	4.011115672585336
5	1.413013328444698	3.565307790328678
6	1.0308120563164853	3.121127463499698
7	0.7736626086566276	2.732941988786351
8	0.5469372472957009	2.208046692100941
9	0.41787054730761986	1.8978637765210102
>10	1.2843857807938843	9.445002407911474
>50	0.04445703893295635	1.5761703969826026
>100	0.030881891868758747	3.145924962750235
>500	0.002360399355050776	0.8111727719560125
>1k	0.001180199677525388	1.5563360121755163
>5k	7.867997850169253E-4	2.372670431725963
>10k+	7.867997850169253E-4	10.741190119109167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	30731	2.9985120136602026	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28376	2.7687278936455666	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG	26383	2.5742651542871084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	22720	2.2168557141114773	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	7672	0.7485790950115868	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTC	5687	0.554896938651055	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	5403	0.5271862422246616	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTT	5141	0.5016221490425662	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	4706	0.4591779485303086	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA	3951	0.3855104281009879	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	3069	0.2994511525795829	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGT	1778	0.17348457128918163	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT	1155	0.11269667032564948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.757287474082205E-5	0.0	0.0	0.06469081595316502	0.0
2	9.757287474082205E-5	0.0	0.0	0.6729601170874497	0.0
3	9.757287474082205E-5	0.0	0.0	0.7793145505549457	0.0
4	9.757287474082205E-5	0.0	0.0	1.6887913160141481	0.0
5	9.757287474082205E-5	0.0	0.0	5.280546408098549	0.0
6	9.757287474082205E-5	0.0	0.0	5.719819490181729	0.0
7	9.757287474082205E-5	0.0	0.0	6.336089767044761	0.0
8	9.757287474082205E-5	0.0	0.0	7.0712038053421145	0.0
9	9.757287474082205E-5	0.0	0.0	7.1965849493840715	0.0
10	9.757287474082205E-5	0.0	0.0	10.268862056348334	0.0
11	9.757287474082205E-5	0.0	0.0	10.4473228442493	0.0
12	9.757287474082205E-5	0.0	0.0	13.180729357238688	0.0
13	9.757287474082205E-5	0.0	0.0	13.388364434687157	0.0
14	9.757287474082205E-5	0.0	0.0	13.569362117331382	0.0
15	9.757287474082205E-5	0.0	0.0	14.082693011342847	0.0
16	9.757287474082205E-5	0.0	0.0	14.358726673984632	0.0
17	9.757287474082205E-5	0.0	0.0	14.508598609586535	0.0
18	9.757287474082205E-5	0.0	0.0	14.626954506647152	0.0
19	9.757287474082205E-5	0.0	0.0	15.358165629954872	0.0
20	9.757287474082205E-5	0.0	0.0	15.50628125381144	0.0
21	9.757287474082205E-5	0.0	0.0	15.626393462617392	0.0
22	9.757287474082205E-5	0.0	0.0	15.877643615075009	0.0
23	9.757287474082205E-5	0.0	0.0	16.007220392730822	0.0
24	9.757287474082205E-5	0.0	0.0	16.121380656177582	0.0
25	9.757287474082205E-5	0.0	0.0	16.21622149042566	0.0
26	9.757287474082205E-5	0.0	0.0	16.346871569703623	0.0
27	3.902914989632882E-4	0.0	0.0	16.51616050737895	0.0
28	4.8786437370411026E-4	0.0	0.0	16.61734357848518	0.0
29	4.8786437370411026E-4	0.0	0.0	16.735016465422614	0.0
30	4.8786437370411026E-4	0.0	0.0	16.862056348335162	9.757287474082205E-5
31	4.8786437370411026E-4	0.0	0.0	16.991535553116233	9.757287474082205E-5
32	4.8786437370411026E-4	0.0	0.0	17.13584583485791	9.757287474082205E-5
33	4.8786437370411026E-4	0.0	0.0	17.26864251738017	9.757287474082205E-5
34	4.8786437370411026E-4	0.0	0.0	17.43715087205757	9.757287474082205E-5
35	4.8786437370411026E-4	0.0	0.0	17.578436394682278	9.757287474082205E-5
36	4.8786437370411026E-4	0.0	0.0	17.7238199780461	9.757287474082205E-5
37	4.8786437370411026E-4	0.0	0.0	17.87886327600927	9.757287474082205E-5
38	4.8786437370411026E-4	0.0	0.0	18.034004146847177	9.757287474082205E-5
39	4.8786437370411026E-4	0.0	0.0	18.20992804000488	9.757287474082205E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAC	25	3.8905095E-5	45.0	3
CGTTTTT	33680	0.0	44.525684	1
GATACCT	3200	0.0	42.75	5
TACGGCT	2815	0.0	42.5222	7
ACGGCTG	2830	0.0	42.217316	8
TGATACC	3235	0.0	42.148376	4
CGGCTGT	2865	0.0	42.09424	9
CCGATGA	850	0.0	42.088238	18
CGATGAA	855	0.0	41.842106	19
ATACCTG	3305	0.0	41.732224	6
GAATCTG	3650	0.0	41.547943	1
CGCTTTT	1025	0.0	41.48781	1
AATCTGT	3735	0.0	40.78313	2
TCTCGCG	50	1.0804797E-9	40.5	1
CGACCTG	245	0.0	40.408165	12
TACCTGT	3465	0.0	40.064934	7
CACCGAG	135	0.0	40.0	16
ATGATAC	10360	0.0	39.57046	3
CACCGGT	245	0.0	39.489796	16
GAATGAT	10800	0.0	39.479168	1
>>END_MODULE