##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933423.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 596409 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.500264080521923 31.0 28.0 31.0 16.0 33.0 2 28.624195811934428 31.0 30.0 33.0 16.0 34.0 3 28.757262214352902 31.0 30.0 33.0 16.0 34.0 4 32.13851065292442 35.0 30.0 37.0 19.0 37.0 5 33.76496833548789 35.0 33.0 37.0 30.0 37.0 6 32.75962468708554 35.0 33.0 37.0 17.0 37.0 7 32.57392326406878 35.0 32.0 36.0 26.0 37.0 8 33.01840683155351 35.0 32.0 37.0 27.0 37.0 9 33.32140192384756 35.0 32.0 38.0 25.0 39.0 10 32.4165597769316 35.0 29.0 39.0 17.0 39.0 11 33.23361652825494 37.0 31.0 39.0 18.0 39.0 12 33.89514410412988 37.0 31.0 39.0 26.0 39.0 13 34.16876505887738 37.0 32.0 39.0 27.0 39.0 14 34.32162995528237 37.0 32.0 40.0 24.0 41.0 15 34.7891011034374 37.0 32.0 40.0 27.0 41.0 16 34.88071105566817 37.0 32.0 40.0 26.0 41.0 17 32.967500490435256 36.0 30.0 40.0 19.0 41.0 18 33.48847016057773 36.0 31.0 39.0 24.0 39.0 19 33.24111976848103 37.0 32.0 37.0 24.0 39.0 20 32.492507658335136 35.0 31.0 36.0 25.0 39.0 21 32.87974695217544 35.0 32.0 37.0 25.0 39.0 22 32.90982865785057 34.0 31.0 37.0 25.0 39.0 23 33.89146709724367 35.0 33.0 38.0 27.0 40.0 24 32.184370121845916 35.0 31.0 37.0 21.0 39.0 25 31.447959370163762 35.0 30.0 37.0 18.0 39.0 26 30.363252398940997 33.0 25.0 37.0 18.0 39.0 27 32.349250262823 34.0 30.0 37.0 22.0 39.0 28 32.326641616742876 35.0 31.0 37.0 21.0 39.0 29 32.33077971660387 35.0 31.0 38.0 21.0 40.0 30 31.44204396647267 35.0 29.0 37.0 18.0 40.0 31 31.635417976589892 35.0 30.0 37.0 19.0 39.0 32 31.23187611186283 35.0 30.0 37.0 16.0 40.0 33 30.553125455853284 35.0 26.0 38.0 12.0 40.0 34 29.13313514718926 35.0 22.0 38.0 7.0 40.0 35 28.417817303226478 34.0 18.0 38.0 7.0 40.0 36 27.57263723384456 33.0 16.0 38.0 7.0 40.0 37 27.688852783911713 34.0 15.0 38.0 7.0 40.0 38 27.662466528841783 34.0 15.0 38.0 7.0 40.0 39 27.168308996007774 33.0 15.0 38.0 7.0 40.0 40 27.08919550174461 33.0 15.0 38.0 7.0 40.0 41 26.435273444901068 32.0 15.0 37.0 7.0 40.0 42 26.973073846974142 33.0 15.0 38.0 7.0 40.0 43 26.84592117154503 33.0 15.0 38.0 7.0 40.0 44 26.955912804803415 33.0 13.0 38.0 7.0 40.0 45 27.255074956950683 34.0 15.0 38.0 7.0 40.0 46 26.897327169777785 33.0 12.0 38.0 7.0 40.0 47 26.94087279031671 33.0 12.0 38.0 7.0 40.0 48 26.666086527869297 33.0 10.0 37.0 7.0 40.0 49 26.551893080084305 33.0 10.0 37.0 7.0 40.0 50 26.608013963571977 33.0 10.0 38.0 7.0 40.0 51 25.092176677414326 30.0 10.0 36.0 7.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 21.0 11 25.0 12 45.0 13 75.0 14 113.0 15 252.0 16 638.0 17 1283.0 18 2342.0 19 4118.0 20 6436.0 21 9235.0 22 13242.0 23 18418.0 24 25608.0 25 34740.0 26 40579.0 27 37577.0 28 31901.0 29 30264.0 30 32615.0 31 37468.0 32 42639.0 33 48567.0 34 53779.0 35 54559.0 36 42297.0 37 22868.0 38 4619.0 39 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.475515963038788 2.1414834450855036 31.0357489575107 44.34725163436501 2 45.581807115586784 13.07592608428109 29.455122239939367 11.887144560192754 3 9.986267812859968 13.018582885234798 64.82648652183317 12.168662780072065 4 9.144228205811784 2.250972067825938 74.51379841685824 14.091001309504048 5 17.436691934561686 2.860285475235954 65.19871430511613 14.504308285086239 6 11.233733897375794 10.728879007526713 67.858801594208 10.17858550088949 7 46.12824420825306 0.8616570172482306 50.51013650028755 2.499962274211154 8 46.71056271786643 7.919565264776353 41.77519118591436 3.594680831442852 9 43.01108802851734 2.170322714781299 41.593269048589136 13.225320208112217 10 23.648368820725373 12.70168625892634 50.172616442743156 13.477328477605132 11 18.505756955377937 15.348695274551524 52.89155596243518 13.253991807635366 12 13.699323786193704 13.081123859633239 56.93861091968766 16.280941434485396 13 13.706701273790301 14.29019347461222 61.434686599296796 10.568418652300686 14 13.07659676497169 17.308256582311802 54.8516202807134 14.763526372003104 15 10.208598461793835 15.195612406922097 61.36879222144535 13.226996909838718 16 10.97384512976833 14.87402101577944 54.18077191994085 19.971361934511382 17 11.891839325026952 16.389424036189933 57.16362429138393 14.555112347399184 18 12.044419182138432 15.115969074913357 55.95656671847675 16.883045024471464 19 12.662451438526245 17.88990441123457 52.964157147192616 16.48348700304657 20 14.051431148758653 16.250760803408397 57.83765838543684 11.860149662396108 21 14.30478077963277 19.444039241527207 53.81642463477244 12.434755344067577 22 12.586832190661108 14.08295314121685 55.79258528962507 17.537629378496973 23 12.969120184303055 19.328849832916674 54.09475712137141 13.607272861408864 24 14.00062708644571 15.640609045135134 53.25489722656768 17.103866641851482 25 11.739427138088123 20.900087020819605 51.79247798071458 15.568007860377694 26 12.232209775506405 15.653184308083882 55.885474565273164 16.22913135113655 27 13.883257965590728 17.065637842487284 52.297332870563665 16.75377132135833 28 11.641004746742588 16.273060936370847 56.48523077284213 15.600703544044439 29 14.319032744308016 13.955691480175517 55.18092449979795 16.54435127571851 30 11.968129253582692 17.440716018705285 53.92809296975734 16.663061757954694 31 14.036340833220157 14.867314208873442 51.30170738536809 19.79463757253831 32 14.747094695083407 17.827866447354083 52.684483299212445 14.740555558350058 33 13.835471966385485 15.652513627393283 49.8151436346534 20.69687077156783 34 18.375812571574205 17.946073919072315 46.91797072143446 16.760142787919026 35 16.141607521013267 18.77050815799225 43.09710282708678 21.990781493907704 36 16.16122493121331 18.079539376501696 44.159125700651735 21.60010999163326 37 16.04352047001303 20.48392965230236 44.13581954665339 19.33673033103122 38 17.645441299510907 20.694691059323382 41.905806250408695 19.754061390757013 39 20.803509001373218 20.322295605867787 41.981090157928534 16.89310523483046 40 20.610352962480448 20.620413172839445 41.826665928917905 16.942567935762202 41 19.525191605089795 21.967643010082007 40.87010759394979 17.63705779087841 42 19.377809523330466 21.43294282950123 41.689679397862875 17.499568249305426 43 18.766484073848652 20.306366939466038 41.16587777850435 19.761271208180965 44 19.326167110154273 21.24179883268026 37.94275404965384 21.489280007511624 45 17.514658564843923 20.468336326245915 38.42312909429603 23.593876014614132 46 20.450730958117667 22.396375641547998 37.61915061643939 19.533742783894944 47 16.41474223226008 19.754229060929664 44.07193721087375 19.759091495936513 48 19.397259263357864 21.356820571118142 36.872347667456395 22.373572498067603 49 17.381360777587194 19.86690341695045 40.94187042784398 21.80986537761838 50 18.068473145106797 19.033247318534762 40.36139629012976 22.53688324622868 51 18.397442023846054 19.183312123056492 37.741885182819175 24.677360670278283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 292.0 2 535.0 3 21782.5 4 43030.0 5 31573.5 6 20117.0 7 19636.5 8 19156.0 9 19206.5 10 19257.0 11 18927.5 12 18598.0 13 17819.0 14 17040.0 15 16329.5 16 15619.0 17 14838.5 18 14058.0 19 13173.0 20 12288.0 21 11450.5 22 10613.0 23 10139.0 24 9665.0 25 9089.5 26 8260.5 27 8007.0 28 8193.0 29 8379.0 30 8525.0 31 8671.0 32 9310.0 33 9949.0 34 10744.0 35 11539.0 36 12659.5 37 13780.0 38 14474.5 39 15169.0 40 17073.5 41 18978.0 42 20323.0 43 21668.0 44 24187.5 45 26707.0 46 49007.0 47 71307.0 48 57658.5 49 44010.0 50 39990.5 51 35971.0 52 30017.0 53 24063.0 54 21236.0 55 18409.0 56 16605.0 57 14801.0 58 12964.5 59 11128.0 60 10004.0 61 8880.0 62 7487.5 63 6095.0 64 5030.5 65 3966.0 66 3198.0 67 2430.0 68 1990.0 69 1550.0 70 1260.0 71 970.0 72 798.0 73 626.0 74 505.0 75 309.0 76 234.0 77 169.0 78 104.0 79 80.5 80 57.0 81 37.5 82 18.0 83 14.5 84 11.0 85 7.5 86 4.0 87 2.0 88 0.0 89 0.5 90 1.0 91 2.0 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 596409.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.85005807293963 #Duplication Level Percentage of deduplicated Percentage of total 1 83.23342741833866 45.653583275056825 2 7.477647864731201 8.202988392589988 3 3.3308627463907405 5.480940452175701 4 1.8780648707357521 4.1204786889841545 5 1.1803253691233384 3.2370457520689513 6 0.7681063949596851 2.527840822184102 7 0.519651672613092 1.9952047094369814 8 0.3800802937707341 1.6677940948563779 9 0.270992343233725 1.3377551187322627 >10 0.886062831392352 7.495248238638694 >50 0.03676594690589903 1.4041104262228357 >100 0.031157580167913656 3.496262137982969 >500 0.003115758016791352 0.9797668802940812 >1k 0.0024926064134330817 2.557340240139653 >5k 6.231516033582704E-4 3.091401203146631 >10k+ 6.231516033582704E-4 6.752239567489822 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27185 4.558113643489619 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG 12325 2.066534877910964 No Hit GAATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC 9172 1.537870823545587 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC 8917 1.495114929519843 No Hit GAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT 3119 0.5229632684952775 No Hit GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC 2719 0.45589519943528684 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTT 2332 0.39100684261974583 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTC 2075 0.3479156082487018 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA 1298 0.21763588409966986 No Hit GAATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT 1263 0.21176742805692067 No Hit GAATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT 1138 0.19080865647567358 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCTCCTTGGTTCGTATGCCG 1020 0.1710235761029763 No Hit GAATCTTTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC 728 0.1220638856891831 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6767017264997678E-4 0.0 0.0 0.1133450367113843 0.0 2 1.6767017264997678E-4 0.0 0.0 0.7701091029813434 0.0 3 1.6767017264997678E-4 0.0 0.0 0.9838885731100637 0.0 4 1.6767017264997678E-4 0.0 0.0 1.8646599900403917 0.0 5 1.6767017264997678E-4 0.0 0.0 3.963387541100151 0.0 6 1.6767017264997678E-4 0.0 0.0 4.560796366252018 0.0 7 1.6767017264997678E-4 0.0 0.0 5.085268666301146 0.0 8 1.6767017264997678E-4 0.0 0.0 5.8025616648977465 0.0 9 1.6767017264997678E-4 0.0 0.0 6.051384201110312 0.0 10 1.6767017264997678E-4 0.0 0.0 8.786420057376732 0.0 11 1.6767017264997678E-4 0.0 0.0 9.368403226644803 0.0 12 1.6767017264997678E-4 0.0 0.0 11.584332228386895 0.0 13 1.6767017264997678E-4 0.0 0.0 11.857634609806357 0.0 14 1.6767017264997678E-4 0.0 0.0 12.031005568326433 0.0 15 1.6767017264997678E-4 0.0 0.0 12.427210186298328 0.0 16 1.6767017264997678E-4 0.0 0.0 12.712584820148589 0.0 17 1.6767017264997678E-4 0.0 0.0 12.987899243639852 0.0 18 1.6767017264997678E-4 0.0 0.0 13.272435526626861 0.0 19 3.3534034529995356E-4 0.0 0.0 13.997106012820062 0.0 20 3.3534034529995356E-4 0.0 0.0 14.246934570068527 0.0 21 3.3534034529995356E-4 0.0 0.0 14.533818235472637 0.0 22 3.3534034529995356E-4 0.0 0.0 14.936561990177882 0.0 23 3.3534034529995356E-4 0.0 0.0 15.255638328730788 0.0 24 3.3534034529995356E-4 0.0 0.0 15.501945812353602 0.0 25 3.3534034529995356E-4 0.0 0.0 15.716563633345574 0.0 26 3.3534034529995356E-4 0.0 0.0 15.938558941934142 0.0 27 3.3534034529995356E-4 0.0 0.0 16.154182783962014 0.0 28 3.3534034529995356E-4 0.0 0.0 16.344153089574437 0.0 29 3.3534034529995356E-4 0.0 0.0 16.565980727990354 0.0 30 3.3534034529995356E-4 0.0 0.0 16.798706927628523 0.0 31 3.3534034529995356E-4 0.0 0.0 17.02690603260514 0.0 32 3.3534034529995356E-4 0.0 0.0 17.250242702574912 0.0 33 3.3534034529995356E-4 0.0 0.0 17.47408238306263 0.0 34 3.3534034529995356E-4 0.0 0.0 17.707311593218748 0.0 35 3.3534034529995356E-4 0.0 0.0 17.936852059576566 0.0 36 3.3534034529995356E-4 0.0 0.0 18.159518048855734 0.0 37 3.3534034529995356E-4 0.0 0.0 18.39744202384605 0.0 38 3.3534034529995356E-4 0.0 0.0 18.62916220244832 0.0 39 3.3534034529995356E-4 0.0 0.0 18.871445601927537 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCGG 30 2.1637388E-6 45.000004 2 GCGACCG 95 0.0 45.0 11 GCGATCG 25 3.888541E-5 45.0 9 ATCCGGC 55 1.8189894E-12 44.999996 6 CGTTTTT 21600 0.0 44.625 1 CGGCTGT 1280 0.0 42.363285 9 CTACGGC 85 0.0 42.35294 6 TACGGCT 1355 0.0 42.17712 7 ACGGCTG 1360 0.0 42.022057 8 TGATCCG 75 0.0 42.0 4 GATACCT 1670 0.0 41.63174 5 TGATACC 1685 0.0 41.52819 4 CCGATGA 180 0.0 41.250004 18 GATCCGG 60 3.6379788E-12 41.250004 5 TCGCTTG 60 3.6379788E-12 41.250004 1 GAATGAT 4890 0.0 41.134968 1 GTTTGCG 55 6.002665E-11 40.90909 1 ATGATAC 4600 0.0 40.891304 3 AATGATA 4565 0.0 40.859802 2 CGACCAC 840 0.0 40.714287 12 >>END_MODULE