SRR2933422.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933422.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281922
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	10664	3.782606536559758	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	9826	3.485361199196941	RNA PCR Primer, Index 11 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	9472	3.359794553103341	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7418	2.631224239328609	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCT	2243	0.7956101332992814	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTC	2122	0.7526904604819773	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	1977	0.7012577947091748	TruSeq Adapter, Index 11 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	1891	0.6707529032853058	TruSeq Adapter, Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1820	0.6455686324586233	Illumina PCR Primer Index 11 (100% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTA	1743	0.6182561133930662	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTACATCTCGT	693	0.24581267159001427	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	570	0.20218358269308534	TruSeq Adapter, Index 11 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT	522	0.18515759678208865	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATG	422	0.14968679280084562	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	287	0.10180120742616751	TruSeq Adapter, Index 11 (96% over 27bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAGTC	20	7.0242205E-4	45.0	22
GATACCG	20	7.0242205E-4	45.0	27
CGTTCAT	40	6.7830115E-9	45.0	17
GCCGCTA	20	7.0242205E-4	45.0	10
ATATCTC	20	7.0242205E-4	45.0	24
CGCTAAC	20	7.0242205E-4	45.0	12
CGAGGCG	20	7.0242205E-4	45.0	9
GCGGGTT	20	7.0242205E-4	45.0	5
CCGAGTT	20	7.0242205E-4	45.0	32
TCCACCG	20	7.0242205E-4	45.0	38
TAGGGTG	20	7.0242205E-4	45.0	5
CGGGATC	75	0.0	45.0	6
CGGCTAT	20	7.0242205E-4	45.0	31
AAGGCCG	20	7.0242205E-4	45.0	28
GTAAACC	20	7.0242205E-4	45.0	25
TACCGAT	20	7.0242205E-4	45.0	16
CGACCCT	30	2.1596534E-6	44.999996	12
CGTTTTT	6490	0.0	44.133278	1
CGATGAA	415	0.0	43.915665	19
GCGACTG	255	0.0	43.235294	11