FastQCFastQC Report
Sat 14 Jan 2017
SRR2933421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197413
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112695.708337343538672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG53052.6872597042747945No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC39411.9963224306403329No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC33451.6944172876153039RNA PCR Primer, Index 11 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC14070.7127190205305629Illumina PCR Primer Index 11 (100% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCT12020.6088758085840345No Hit
GAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT9640.48831637227538205TruSeq Adapter, Index 11 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTC8610.43614149017541903No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTA7150.36218486117935494No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT5580.2826561573959162TruSeq Adapter, Index 11 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT4320.21883057346780607TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTACATCTCGTATGCCG4170.2112322896668406No Hit
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC3190.16159016883386604Illumina PCR Primer Index 11 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC2760.139808421937765RNA PCR Primer, Index 11 (96% over 26bp)
GAATCTTTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC2710.13727566067077648Illumina PCR Primer Index 11 (100% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTACATCTCGT2660.134742899403788No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT2640.1337297948969926TruSeq Adapter, Index 11 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2490.1261315110960271No Hit
GAATGACTCTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT2280.11549391377467544TruSeq Adapter, Index 11 (95% over 21bp)
GAATCTGTCTCTTATCCACATCTGACGCGGTACATCTCGTATGCCGTCTTC2250.11397425701448233Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA2230.11296115250768693No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC2010.10181700293293754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC253.8790356E-545.017
ACACGTG453.8198777E-1045.042
GATGTTG207.01908E-445.011
TTGTGCG253.8790356E-545.01
GACACGA453.8198777E-1045.025
ACCGCCT207.01908E-445.014
GACCGAT351.2050987E-745.09
GATCCCT253.8790356E-545.05
GTCTCCT207.01908E-445.014
ACCGAGG207.01908E-445.017
AACGGGC207.01908E-445.029
TAATTGC253.8790356E-545.030
ACGTGAG453.8198777E-1045.044
CGGGATA453.8198777E-1045.06
TAGGGCG253.8790356E-545.05
CCGGTCT253.8790356E-545.018
CGGTCTC253.8790356E-545.019
ACCCGGG207.01908E-445.039
GGACCGA351.2050987E-745.08
ATTGCAA253.8790356E-545.032
Produced by FastQC (version 0.11.5)