##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933421.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197413
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.95189273249482	31.0	25.0	31.0	16.0	33.0
2	28.03642617254183	31.0	28.0	33.0	16.0	34.0
3	28.204398899768506	31.0	28.0	33.0	16.0	34.0
4	31.428279799202688	35.0	28.0	35.0	19.0	37.0
5	33.437858702314436	35.0	33.0	37.0	28.0	37.0
6	32.099664155856	35.0	32.0	37.0	17.0	37.0
7	32.045792323707154	35.0	32.0	35.0	25.0	37.0
8	32.41535258569598	35.0	32.0	35.0	25.0	37.0
9	32.8494374737226	35.0	31.0	37.0	25.0	39.0
10	31.71155395034775	35.0	28.0	39.0	15.0	39.0
11	32.32199500539478	35.0	28.0	39.0	17.0	39.0
12	33.15116025793641	35.0	31.0	39.0	23.0	39.0
13	33.258108635196265	35.0	31.0	39.0	24.0	39.0
14	33.30098321792384	36.0	31.0	39.0	18.0	41.0
15	33.908070897053385	36.0	32.0	40.0	24.0	41.0
16	33.99623125123472	36.0	32.0	40.0	25.0	41.0
17	31.96558483990416	34.0	27.0	39.0	18.0	41.0
18	32.741926823461476	35.0	30.0	39.0	21.0	39.0
19	32.523091184471134	35.0	30.0	37.0	19.0	39.0
20	32.10164984068932	34.0	30.0	36.0	23.0	39.0
21	32.57234832559153	35.0	31.0	37.0	23.0	39.0
22	32.91104435878083	34.0	31.0	37.0	25.0	39.0
23	33.852436263062714	35.0	32.0	38.0	27.0	40.0
24	32.302214139899604	35.0	31.0	37.0	21.0	39.0
25	31.452655093636185	34.0	29.0	37.0	18.0	39.0
26	30.819712987493226	33.0	25.0	37.0	19.0	39.0
27	32.38514180930334	34.0	30.0	37.0	22.0	39.0
28	32.25438547613379	35.0	30.0	37.0	21.0	39.0
29	32.484228495590465	35.0	31.0	38.0	21.0	40.0
30	31.584302958771712	35.0	30.0	37.0	18.0	40.0
31	31.761439216262353	35.0	30.0	37.0	20.0	39.0
32	31.771058643554376	35.0	30.0	37.0	18.0	40.0
33	31.72151783317208	35.0	30.0	38.0	16.0	40.0
34	30.8905999098337	35.0	29.0	38.0	12.0	40.0
35	30.35931270990259	35.0	27.0	38.0	10.0	40.0
36	29.528252951933258	34.0	24.0	38.0	8.0	40.0
37	29.675107515715784	35.0	24.0	38.0	8.0	40.0
38	29.39937086210128	34.0	23.0	38.0	8.0	40.0
39	28.98104481467786	34.0	23.0	37.0	7.0	40.0
40	29.047666567044722	34.0	23.0	38.0	8.0	40.0
41	28.312167891678865	33.0	21.0	37.0	8.0	40.0
42	28.94836712881117	34.0	22.0	38.0	7.0	40.0
43	28.95208015682858	34.0	22.0	38.0	7.0	40.0
44	28.87009467461616	34.0	22.0	38.0	7.0	40.0
45	28.957322972651244	34.0	22.0	38.0	7.0	40.0
46	28.6227654713722	34.0	20.0	38.0	7.0	40.0
47	28.66960129272135	34.0	21.0	38.0	7.0	40.0
48	28.407232553074014	34.0	20.0	37.0	7.0	40.0
49	28.2885422945804	34.0	20.0	37.0	7.0	40.0
50	28.445558296566084	34.0	20.0	37.0	7.0	40.0
51	26.715793792708688	31.0	18.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	13.0
11	16.0
12	20.0
13	22.0
14	50.0
15	101.0
16	255.0
17	428.0
18	696.0
19	1192.0
20	1776.0
21	2563.0
22	3224.0
23	4515.0
24	6171.0
25	8359.0
26	10719.0
27	11657.0
28	11494.0
29	11573.0
30	12841.0
31	15056.0
32	16462.0
33	18235.0
34	18974.0
35	19558.0
36	13362.0
37	6804.0
38	1241.0
39	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.359393758263135	2.477040519114749	31.478676682893227	38.684889039728894
2	38.924488255586006	15.972605654136252	31.376859679960283	13.726046410317455
3	12.598967646507575	16.072903000308997	58.77981693201563	12.548312421167807
4	11.78544472755087	2.885828187606693	70.26133030752787	15.067396777314565
5	21.564942531646853	3.3543890219995647	58.547815999959475	16.53285244639411
6	13.045746734004346	13.771129560869852	61.11704902919261	12.066074675933196
7	49.33818948093591	1.3256472471417788	46.019259116674185	3.316904155248135
8	48.73539229939264	10.859467208339876	35.2271633580362	5.177977134231282
9	44.91142933849341	2.852902291135842	35.46068394685254	16.77498442351821
10	27.262642277864174	17.454777547577923	41.07834843703302	14.204231737524884
11	23.06484375395744	15.31054185894546	47.730899180905006	13.893715206192095
12	18.527655220274248	14.222467618647203	49.56563144271147	17.684245718367077
13	16.842355873220104	15.694001914767519	56.003404031142836	11.460238180869547
14	13.825330651983405	20.230683896197313	47.596662833754614	18.347322618064666
15	10.511465810255658	15.514175864811335	58.25908121552279	15.715277109410222
16	12.167891678866132	15.60738147943651	48.04648123477177	24.178245606925582
17	12.706356724227888	17.29217427423726	52.903304240348916	17.09816476118594
18	13.02751085288203	15.526839671146279	51.83903795596034	19.606611520011345
19	13.293450785915823	18.695830568402283	47.895528663259256	20.11518998242264
20	16.064798164254633	17.345868813097415	54.035448526692775	12.553884495955181
21	16.51512311752519	22.301469508087106	47.050092952338495	14.133314422049207
22	13.363354996884706	15.253301454311519	50.522002097126325	20.861341451677447
23	14.331882905381105	22.37086716680259	47.809921332435046	15.487328595381255
24	15.583066971273421	16.408240592058274	47.34946533409654	20.659227102571766
25	12.665325991702675	23.726907549148233	45.61452386620942	17.993242592939673
26	14.42762128127327	16.918845263483153	49.84220897306662	18.811324482176957
27	15.989321878498378	18.898451469761362	46.68841464341254	18.42381200832772
28	12.069113989453582	18.303759124272464	52.87392420965185	16.753202676622106
29	19.801127585316063	15.561285224377322	47.115438193026804	17.522148997279814
30	16.302877723351553	20.4322916930496	48.4593213212909	14.805509262307954
31	16.87781453095794	18.250571137665705	45.970630100348004	18.90098423102835
32	18.340230886517098	22.60590741237912	44.196684108949256	14.85717759215452
33	14.947850445512707	18.205994539366706	41.49625404608612	25.34990096903446
34	20.52600385992817	21.776174821313692	41.62187900492875	16.075942313829383
35	18.41266785875297	20.142037251852717	41.20701270939604	20.238282179998276
36	19.261649435447513	19.337125721203773	41.136601946173755	20.26462289717496
37	17.999827772233846	24.397582732646786	41.500813016366706	16.101776478752665
38	16.162056196906992	21.33648746536449	40.34334111735296	22.158115220375556
39	17.451738234057533	27.28695678602726	37.50614194607245	17.755163033842756
40	17.53683901262835	20.14811587889349	42.741866037191066	19.573179071287097
41	16.1823182870429	24.36313717941574	37.0993804865941	22.355164046947262
42	16.96139565276856	20.359348168560327	44.11057022587165	18.56868595279946
43	19.974368455978077	19.0443385187399	36.618662398119675	24.362630627162346
44	20.76813583705227	21.016852993470543	37.43623773510357	20.778773434373623
45	16.909220770668597	20.04629887596055	39.30085657986049	23.743623773510354
46	21.21542147680244	24.758754489319344	34.60258442959683	19.42323960428138
47	15.187956213623217	20.238788732251674	43.637956973451594	20.935298080673512
48	18.613262551098458	23.442225182738728	34.98452482865871	22.959987437504115
49	16.189410018590465	19.706402313930692	41.63099694548991	22.473190721988924
50	18.30021325849868	18.751044764022634	37.513233677620015	25.43550829985867
51	18.383287828055902	19.300653958959135	35.73118285016691	26.58487536281805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	183.5
2	318.0
3	8412.5
4	16507.0
5	11485.5
6	6464.0
7	6465.5
8	6467.0
9	6252.0
10	6037.0
11	5505.0
12	4973.0
13	4433.5
14	3894.0
15	3453.0
16	3012.0
17	2714.0
18	2416.0
19	2269.0
20	2122.0
21	1934.5
22	1747.0
23	1746.5
24	1746.0
25	1769.5
26	1818.5
27	1844.0
28	2017.5
29	2191.0
30	2298.5
31	2406.0
32	2607.0
33	2808.0
34	3305.5
35	3803.0
36	3962.0
37	4121.0
38	4266.0
39	4411.0
40	4889.5
41	5368.0
42	6001.0
43	6634.0
44	7330.0
45	8026.0
46	17215.5
47	26405.0
48	21697.5
49	16990.0
50	15520.5
51	14051.0
52	11513.5
53	8976.0
54	8110.0
55	7244.0
56	6518.5
57	5793.0
58	5320.5
59	4848.0
60	4421.0
61	3994.0
62	3663.0
63	3332.0
64	2797.0
65	2262.0
66	1890.0
67	1518.0
68	1285.5
69	1053.0
70	860.0
71	667.0
72	520.5
73	374.0
74	360.0
75	298.0
76	250.0
77	171.0
78	92.0
79	65.0
80	38.0
81	23.0
82	8.0
83	9.5
84	11.0
85	7.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.42608642794547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29646397627039	37.384062853003606
2	6.531217592024712	5.933753096300649
3	2.620515851333118	3.5711933864537797
4	1.7250800093669503	3.134545344024963
5	1.2723440793068457	2.8898806056338744
6	0.9288892358129732	2.5317481624817004
7	0.8207232623749678	2.6097572095049464
8	0.6289238043199483	2.285563767330419
9	0.559786790369883	2.288603080850805
>10	2.509004538510432	17.320034648174133
>50	0.05798588266779665	1.7693870211181635
>100	0.03791384635971319	3.304240348913192
>500	0.004460452512907435	1.5692988810260722
>1k	0.004460452512907435	5.012334547370234
>5k	0.0011151131282268586	2.6872597042747945
>10k+	0.0011151131282268586	5.708337343538672
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11269	5.708337343538672	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	5305	2.6872597042747945	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	3941	1.9963224306403329	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	3345	1.6944172876153039	RNA PCR Primer, Index 11 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	1407	0.7127190205305629	Illumina PCR Primer Index 11 (100% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCT	1202	0.6088758085840345	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	964	0.48831637227538205	TruSeq Adapter, Index 11 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTC	861	0.43614149017541903	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTA	715	0.36218486117935494	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	558	0.2826561573959162	TruSeq Adapter, Index 11 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	432	0.21883057346780607	TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTACATCTCGTATGCCG	417	0.2112322896668406	No Hit
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	319	0.16159016883386604	Illumina PCR Primer Index 11 (100% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	276	0.139808421937765	RNA PCR Primer, Index 11 (96% over 26bp)
GAATCTTTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	271	0.13727566067077648	Illumina PCR Primer Index 11 (100% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTACATCTCGT	266	0.134742899403788	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	264	0.1337297948969926	TruSeq Adapter, Index 11 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	249	0.1261315110960271	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	228	0.11549391377467544	TruSeq Adapter, Index 11 (95% over 21bp)
GAATCTGTCTCTTATCCACATCTGACGCGGTACATCTCGTATGCCGTCTTC	225	0.11397425701448233	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	223	0.11296115250768693	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC	201	0.10181700293293754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010131045067953985	0.0	0.0	0.28214960514251847	0.0
2	0.0010131045067953985	0.0	0.0	1.4700146393601232	0.0
3	0.0010131045067953985	0.0	0.0	1.7435528561948808	0.0
4	0.0010131045067953985	0.0	0.0	2.696377644835953	0.0
5	0.0010131045067953985	0.0	0.0	5.04627354834788	0.0
6	0.0010131045067953985	0.0	0.0	5.48646745655048	0.0
7	0.0010131045067953985	0.0	0.0	6.067989443451039	0.0
8	0.0010131045067953985	0.0	0.0	6.92203654267956	0.0
9	0.0010131045067953985	0.0	0.0	7.1474522954415365	0.0
10	0.0010131045067953985	0.0	0.0	11.215066890225062	0.0
11	0.0010131045067953985	0.0	0.0	12.079245034521536	0.0
12	0.0010131045067953985	0.0	0.0	15.173266198274684	0.0
13	0.0010131045067953985	0.0	0.0	15.517215178331721	0.0
14	0.0010131045067953985	0.0	0.0	15.724901602224778	0.0
15	0.0010131045067953985	0.0	0.0	16.35150673967773	0.0
16	0.0010131045067953985	0.0	0.0	16.74357818380755	0.0
17	0.0010131045067953985	0.0	0.0	17.147806882018916	0.0
18	0.0010131045067953985	0.0	0.0	17.494288623342943	0.0
19	0.0010131045067953985	0.0	0.0	18.524109354500464	0.0
20	0.0010131045067953985	0.0	0.0	18.903010440041943	0.0
21	0.0010131045067953985	0.0	0.0	19.31331776529408	0.0
22	0.0010131045067953985	0.0	0.0	19.982473292032438	0.0
23	0.0010131045067953985	0.0	0.0	20.354282646026352	0.0
24	0.0010131045067953985	0.0	0.0	20.644030534969836	0.0
25	0.0010131045067953985	0.0	0.0	20.905918049976446	0.0
26	0.0010131045067953985	0.0	0.0	21.142477952313172	0.0
27	0.0010131045067953985	0.0	0.0	21.39170166098484	0.0
28	0.0010131045067953985	0.0	0.0	21.60698636867886	0.0
29	0.0010131045067953985	0.0	0.0	21.83645453946802	0.0
30	0.0010131045067953985	0.0	0.0	22.111005860809573	0.0
31	0.0010131045067953985	0.0	0.0	22.33591506131815	0.0
32	0.0010131045067953985	0.0	0.0	22.548667007745184	0.0
33	0.0010131045067953985	0.0	0.0	22.750781356850865	0.0
34	0.0010131045067953985	0.0	0.0	22.97872987087983	0.0
35	0.0010131045067953985	0.0	0.0	23.235045311099068	0.0
36	0.0010131045067953985	0.0	0.0	23.46350037738143	0.0
37	0.0010131045067953985	0.0	0.0	23.674226114794873	0.0
38	0.0010131045067953985	0.0	0.0	23.898122210796654	0.0
39	0.0010131045067953985	0.0	0.0	24.1741931888984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	25	3.8790356E-5	45.0	17
ACACGTG	45	3.8198777E-10	45.0	42
GATGTTG	20	7.01908E-4	45.0	11
TTGTGCG	25	3.8790356E-5	45.0	1
GACACGA	45	3.8198777E-10	45.0	25
ACCGCCT	20	7.01908E-4	45.0	14
GACCGAT	35	1.2050987E-7	45.0	9
GATCCCT	25	3.8790356E-5	45.0	5
GTCTCCT	20	7.01908E-4	45.0	14
ACCGAGG	20	7.01908E-4	45.0	17
AACGGGC	20	7.01908E-4	45.0	29
TAATTGC	25	3.8790356E-5	45.0	30
ACGTGAG	45	3.8198777E-10	45.0	44
CGGGATA	45	3.8198777E-10	45.0	6
TAGGGCG	25	3.8790356E-5	45.0	5
CCGGTCT	25	3.8790356E-5	45.0	18
CGGTCTC	25	3.8790356E-5	45.0	19
ACCCGGG	20	7.01908E-4	45.0	39
GGACCGA	35	1.2050987E-7	45.0	8
ATTGCAA	25	3.8790356E-5	45.0	32
>>END_MODULE