##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933420.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	474171
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.18399691250625	31.0	28.0	31.0	25.0	34.0
2	28.19610435897598	31.0	25.0	33.0	16.0	34.0
3	28.255367789257463	31.0	25.0	33.0	16.0	34.0
4	32.83492664038923	35.0	32.0	37.0	28.0	37.0
5	34.05261814830515	35.0	33.0	37.0	30.0	37.0
6	33.21700610117447	35.0	32.0	37.0	28.0	37.0
7	33.23309523357607	35.0	32.0	35.0	28.0	37.0
8	33.15330545309604	35.0	32.0	37.0	28.0	37.0
9	34.342597923533916	37.0	33.0	39.0	27.0	39.0
10	34.26795607491812	37.0	32.0	39.0	27.0	39.0
11	34.556075761697784	37.0	33.0	39.0	27.0	39.0
12	34.66404314055478	37.0	33.0	39.0	27.0	39.0
13	34.8096108787758	37.0	33.0	39.0	27.0	39.0
14	35.628870175527396	38.0	34.0	40.0	27.0	41.0
15	35.85757247912673	38.0	34.0	40.0	27.0	41.0
16	35.79564123491314	37.0	34.0	40.0	29.0	41.0
17	35.07647662973906	37.0	33.0	40.0	27.0	41.0
18	34.894896566850356	37.0	33.0	39.0	27.0	40.0
19	34.38268261871772	37.0	32.0	38.0	27.0	40.0
20	33.84965550402703	35.0	32.0	38.0	26.0	40.0
21	34.447119288189285	35.0	33.0	39.0	27.0	40.0
22	35.089497248882786	36.0	33.0	39.0	29.0	40.0
23	35.31535458726915	36.0	34.0	39.0	29.0	40.0
24	34.91409217349859	36.0	33.0	39.0	27.0	40.0
25	33.8954216938615	35.0	33.0	39.0	24.0	40.0
26	34.04186675271158	35.0	33.0	39.0	25.0	40.0
27	34.67305887538462	36.0	33.0	39.0	27.0	40.0
28	34.31452999023559	36.0	33.0	39.0	25.0	40.0
29	34.482323887373965	36.0	33.0	39.0	25.0	40.0
30	33.837796912928034	35.0	33.0	39.0	23.0	40.0
31	33.93179675686619	36.0	33.0	39.0	24.0	40.0
32	33.61991349112451	36.0	33.0	39.0	21.0	40.0
33	33.28028285154512	36.0	33.0	40.0	18.0	41.0
34	33.14585666352434	37.0	33.0	40.0	15.0	41.0
35	32.80073011635043	37.0	32.0	40.0	12.0	41.0
36	32.37502504370786	37.0	31.0	40.0	10.0	41.0
37	32.39531730114241	37.0	31.0	40.0	10.0	41.0
38	32.129752768516	37.0	31.0	40.0	10.0	41.0
39	31.90124870563573	36.0	30.0	40.0	10.0	40.0
40	31.661457575431648	36.0	30.0	40.0	10.0	40.0
41	31.130634307032693	35.0	28.0	39.0	8.0	40.0
42	31.510547882514956	36.0	30.0	39.0	8.0	40.0
43	31.41530797961073	36.0	29.0	39.0	8.0	40.0
44	31.589930636837764	36.0	30.0	40.0	8.0	40.0
45	31.736078334609246	37.0	30.0	40.0	8.0	40.0
46	31.45957681933311	36.0	29.0	40.0	8.0	40.0
47	31.458583506793964	36.0	30.0	39.0	8.0	40.0
48	31.228862583329644	36.0	29.0	39.0	8.0	40.0
49	31.489698863911965	36.0	29.0	40.0	8.0	40.0
50	31.38722106581803	36.0	29.0	40.0	8.0	40.0
51	30.21035449236668	35.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	35.0
10	40.0
11	32.0
12	50.0
13	59.0
14	92.0
15	126.0
16	208.0
17	424.0
18	873.0
19	1534.0
20	2438.0
21	3450.0
22	4967.0
23	6827.0
24	9445.0
25	13479.0
26	17769.0
27	19432.0
28	18490.0
29	18105.0
30	19896.0
31	23106.0
32	28321.0
33	34263.0
34	40704.0
35	47442.0
36	54548.0
37	57121.0
38	44308.0
39	6578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.699745450480943	2.882504412964943	33.959056964681515	31.458693171872593
2	30.326612129379487	20.05816467055134	35.239607652091756	14.375615547977416
3	13.13260406056043	20.26673921433407	51.97302239065653	14.627634334448963
4	11.981331629306727	3.20622728931124	67.81561925971855	16.996821821663495
5	25.168979123565126	4.015639927367975	51.863568206406555	18.951812742660348
6	14.89525930518737	18.377969129280363	55.40174325296149	11.325028312570781
7	57.31350082565151	1.432816431203089	38.20288461335679	3.0507981297886206
8	55.574887540570806	14.097024069375816	26.571637658144425	3.756450731908953
9	52.27207062431064	3.502744790381529	25.34802845387002	18.877156131437815
10	30.875991994449258	21.686901982618085	32.614605279529954	14.822500743402697
11	26.304223581788005	17.774178513658576	40.399560496107945	15.522037408445478
12	20.288250441296494	15.106575475935896	43.3120119113147	21.293162171452913
13	19.137610693188744	16.253419125167927	50.89324315489559	13.715727026747734
14	16.955275628412537	23.079859375626093	40.47084279721872	19.49402219874265
15	13.271372563906269	17.142760733996806	52.124444556921446	17.461422145175476
16	15.042885372576558	18.859019214587143	40.47991125564406	25.618184157192236
17	15.412583224195492	19.499083663910277	46.597746382634114	18.49058672926012
18	14.934696554618482	18.432590774214365	44.80366787509148	21.829044796075678
19	15.731244635374159	21.456183528726978	40.81839673872928	21.994175097169585
20	18.60025180789209	19.73528537173298	47.26311815779539	14.401344662579532
21	18.668581587655087	25.879904085235072	40.424867821946094	15.026646505163749
22	16.28737312066744	16.673942522845135	43.96409734041095	23.074587016076478
23	16.75619133181911	24.966309622477965	42.0936750665899	16.183823979113022
24	18.507458279818884	18.971004131420944	40.1966801006388	22.324857488121374
25	14.995855925394002	28.326489810637934	38.41567704477921	18.261977219188857
26	15.651315664602011	18.12109977202317	43.90504691345527	22.322537649919543
27	18.70148111124468	20.69527659852669	39.568003947942834	21.0352383422858
28	13.456116042524743	20.96121441420922	46.21539486809611	19.36727467516993
29	19.49317862121471	16.87218324191062	40.31203932758435	23.32259880929032
30	15.323374900616022	23.151563465500843	45.433820288461334	16.0912413454218
31	18.289815277610817	17.878782127122918	40.19204042423514	23.639362171031124
32	20.115317048069155	22.43304630607945	37.67058719322776	19.78104945262363
33	17.032673866600867	18.701692005626665	39.24512464912448	25.020509478648002
34	19.064219448258118	20.321149964886086	35.56986825427958	25.04476233257622
35	16.42403268019343	20.26821547500796	42.324604414862996	20.983147429935613
36	19.924246737991147	21.192565551246282	36.61948959341672	22.26369811734585
37	16.455666837491115	22.094982611758205	39.79007573217257	21.65927481857811
38	18.617123358450854	22.069464391538073	39.22930757047563	20.084104679535443
39	19.74351025263038	22.097091555578054	32.13692950433493	26.022468687456634
40	17.095942181196236	21.635021964649884	42.579997511446294	18.68903834270759
41	21.27818867033201	23.88885022491886	31.872046160562327	22.960914944186804
42	16.637036005997835	22.239023474653656	41.15561685552259	19.968323663825917
43	22.748122512764382	21.508907124223118	31.794647922373997	23.948322440638503
44	20.680092203023804	21.687956454528006	35.02681522066934	22.605136121778852
45	17.240826621619625	21.38743196020001	36.178720335068995	25.19302108311137
46	23.48224585645263	25.023672894377768	31.228818295509424	20.265262953660176
47	15.649628509546135	21.496675250068016	43.21310244616394	19.640593794221918
48	20.308074513203042	23.58263158227728	31.605264767351866	24.504029137167816
49	16.808704032933267	20.70919562773767	39.93158586248421	22.550514476844853
50	20.70497774009798	20.140624373907304	35.82568313962685	23.328714746367872
51	19.11757572690021	20.3810439693697	33.499307211955184	27.002073091774907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	566.5
2	1081.0
3	11171.5
4	21262.0
5	16480.0
6	11698.0
7	11107.5
8	10517.0
9	10097.5
10	9678.0
11	9100.5
12	8523.0
13	7883.5
14	7244.0
15	6690.0
16	6136.0
17	5695.5
18	5255.0
19	4840.0
20	4425.0
21	4155.5
22	3886.0
23	3840.0
24	3794.0
25	3850.0
26	4537.0
27	5168.0
28	5679.0
29	6190.0
30	6364.0
31	6538.0
32	7587.0
33	8636.0
34	9595.5
35	10555.0
36	11085.5
37	11616.0
38	12736.5
39	13857.0
40	15370.5
41	16884.0
42	17556.0
43	18228.0
44	20341.0
45	22454.0
46	53265.5
47	84077.0
48	58898.0
49	33719.0
50	31159.5
51	28600.0
52	25090.5
53	21581.0
54	20106.0
55	18631.0
56	17341.0
57	16051.0
58	15097.0
59	14143.0
60	13234.0
61	12325.0
62	10691.5
63	9058.0
64	7810.5
65	6563.0
66	5314.5
67	4066.0
68	3415.5
69	2765.0
70	2353.0
71	1941.0
72	1607.0
73	1273.0
74	1070.0
75	668.0
76	469.0
77	348.5
78	228.0
79	172.5
80	117.0
81	95.5
82	74.0
83	47.5
84	21.0
85	17.0
86	13.0
87	8.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	474171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.706013765571655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.05250931861474	32.993245600576564
2	5.8459961022312115	4.759343956218039
3	2.4596946612554156	3.0037309422049816
4	1.7216676378989881	2.803289062722218
5	1.3107687715338128	2.6678085828768414
6	1.1385701874745306	2.7807992234644656
7	0.9749571472871192	2.7780633340818364
8	0.8989073360736047	2.92727475169484
9	0.7365320784894335	2.6983156433198383
>10	3.7634393078248256	22.93159121116728
>50	0.06270617775349321	1.7342111221865797
>100	0.026874076180068335	2.0608905951723644
>500	0.002107770680789673	0.6533589459715885
>1k	0.0031616560211845095	3.664472827635791
>5k	5.269426701974183E-4	1.9967558857680625
>10k+	0.0015808280105922548	9.546848314938758
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	17209	3.629281419572264	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	13733	2.8962125477939393	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	13566	2.860993186002518	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9309	1.9632158018942532	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT	3179	0.6704332403289109	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	3053	0.6438605481988565	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	2796	0.5896606920288251	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2772	0.5845992268611957	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	2715	0.5725782470880758	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTA	2569	0.5417876673183303	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGT	955	0.2014041347952532	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	732	0.15437468761269668	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTAT	705	0.1486805392991136	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATG	654	0.13792492581790114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08056165391810127	0.0
2	0.0	0.0	0.0	0.7741932762653135	0.0
3	0.0	0.0	0.0	0.8874435593910214	0.0
4	0.0	0.0	0.0	1.6122875502719483	0.0
5	0.0	0.0	0.0	4.936404799112556	0.0
6	0.0	0.0	0.0	5.192852367605779	0.0
7	0.0	0.0	0.0	5.931826282079672	0.0
8	0.0	0.0	0.0	6.7463003853040355	0.0
9	0.0	0.0	0.0	6.871149859438894	0.0
10	0.0	0.0	0.0	11.19216485192051	0.0
11	0.0	0.0	0.0	11.381126218178673	0.0
12	0.0	0.0	0.0	14.92394094113727	0.0
13	0.0	0.0	0.0	15.196205588279334	0.0
14	0.0	0.0	0.0	15.419331844418997	0.0
15	0.0	0.0	0.0	16.141645102716108	0.0
16	0.0	0.0	0.0	16.475912698161633	0.0
17	0.0	0.0	0.0	16.63534885094196	0.0
18	0.0	0.0	0.0	16.764838001480477	0.0
19	0.0	0.0	0.0	17.69446043726841	0.0
20	0.0	0.0	0.0	17.889748634986113	0.0
21	0.0	0.0	0.0	18.02661908889409	0.0
22	0.0	0.0	0.0	18.31111561019126	0.0
23	0.0	0.0	0.0	18.45304752926687	0.0
24	0.0	0.0	0.0	18.582325785423404	0.0
25	0.0	0.0	0.0	18.685664032595835	0.0
26	0.0	0.0	0.0	18.818949282010077	0.0
27	0.0	0.0	0.0	19.031530819050513	0.0
28	0.0	0.0	0.0	19.15131882801774	0.0
29	0.0	0.0	0.0	19.281862450466182	0.0
30	0.0	0.0	0.0	19.412195178532638	0.0
31	0.0	0.0	0.0	19.54231701221711	0.0
32	0.0	0.0	0.0	19.69352828410004	0.0
33	0.0	0.0	0.0	19.825548167222372	0.0
34	0.0	0.0	0.0	20.015985794154428	0.0
35	0.0	0.0	0.0	20.169938693003157	0.0
36	0.0	0.0	0.0	20.326633218817683	0.0
37	0.0	0.0	0.0	20.498301245753115	0.0
38	0.0	0.0	0.0	20.667649434486716	0.0
39	2.1089438198455831E-4	0.0	0.0	20.84142640524199	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.887328E-5	45.0	1
TCGATCT	20	7.02909E-4	45.0	18
TCTAGCG	20	7.02909E-4	45.0	1
ACGGGAT	40	6.8012014E-9	45.0	5
GAACGTC	20	7.02909E-4	45.0	9
TCGAGTC	20	7.02909E-4	45.0	15
CCACCGT	20	7.02909E-4	45.0	15
CGTTTTT	8205	0.0	44.259598	1
GATACCT	2175	0.0	43.241383	5
TGATACC	2190	0.0	43.047943	4
ACGGCTG	1630	0.0	42.51534	8
CACCGGT	90	0.0	42.5	16
CGGCTGT	1635	0.0	42.38532	9
TACGGCT	1660	0.0	41.88253	7
GGATCAC	65	0.0	41.538464	8
AGCGGGA	65	0.0	41.538464	4
ATACCTG	2265	0.0	41.52318	6
TACCTGT	2220	0.0	41.4527	7
CGACCAC	1550	0.0	41.370968	12
ACCACCG	945	0.0	41.19048	14
>>END_MODULE