##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933419.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337019
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.22912951495316	31.0	26.0	31.0	16.0	33.0
2	28.458356947234428	31.0	28.0	33.0	16.0	34.0
3	28.691732513597156	31.0	28.0	33.0	16.0	34.0
4	31.669018660668982	35.0	28.0	35.0	19.0	37.0
5	33.39945522359274	35.0	33.0	37.0	28.0	37.0
6	32.25084639144974	35.0	32.0	37.0	17.0	37.0
7	31.567401244440223	33.0	30.0	35.0	23.0	37.0
8	32.49701945587638	35.0	32.0	35.0	25.0	37.0
9	32.37652179847427	34.0	31.0	37.0	25.0	39.0
10	31.115005385453046	35.0	27.0	37.0	15.0	39.0
11	31.85167008388251	35.0	27.0	39.0	17.0	39.0
12	32.769300840605425	35.0	31.0	39.0	23.0	39.0
13	32.943335538945874	35.0	31.0	39.0	23.0	39.0
14	33.12004664425448	36.0	31.0	39.0	18.0	41.0
15	33.66387651734769	36.0	31.0	39.0	24.0	41.0
16	33.82038401395767	36.0	32.0	39.0	24.0	41.0
17	31.656158851578102	34.0	27.0	38.0	17.0	41.0
18	32.486296024853196	35.0	30.0	39.0	19.0	39.0
19	32.44533394259671	35.0	30.0	37.0	18.0	39.0
20	32.24502179402348	34.0	30.0	37.0	23.0	39.0
21	32.77316412427786	35.0	31.0	37.0	23.0	39.0
22	33.27958364365214	35.0	31.0	38.0	25.0	39.0
23	34.19174883315184	35.0	33.0	39.0	27.0	40.0
24	32.525225580753606	35.0	31.0	38.0	22.0	39.0
25	31.621226102979357	35.0	30.0	37.0	18.0	39.0
26	31.295923968678323	34.0	27.0	37.0	20.0	39.0
27	32.74031434429513	35.0	31.0	38.0	22.0	40.0
28	32.57345728282382	35.0	31.0	38.0	21.0	40.0
29	32.97584705906789	35.0	31.0	38.0	22.0	40.0
30	31.881371673407138	35.0	30.0	38.0	18.0	40.0
31	32.151193256166565	35.0	30.0	38.0	21.0	40.0
32	32.19855557105089	35.0	30.0	38.0	20.0	40.0
33	32.35143419213753	35.0	31.0	38.0	18.0	40.0
34	31.808055332191955	35.0	30.0	38.0	15.0	40.0
35	31.363451318768377	35.0	30.0	38.0	12.0	40.0
36	30.842115726413052	35.0	29.0	38.0	10.0	40.0
37	31.044920316065266	35.0	29.0	38.0	10.0	40.0
38	30.934398357362642	35.0	29.0	38.0	10.0	40.0
39	30.50968343031105	35.0	27.0	38.0	9.0	40.0
40	30.270593052617212	35.0	26.0	38.0	9.0	40.0
41	29.658360507864543	34.0	24.0	38.0	8.0	40.0
42	30.25592028936054	35.0	26.0	38.0	8.0	40.0
43	30.31975051851676	35.0	26.0	38.0	8.0	40.0
44	30.318590346538326	35.0	26.0	38.0	8.0	40.0
45	30.62229429201321	35.0	27.0	38.0	8.0	40.0
46	30.345437497589156	35.0	26.0	38.0	8.0	40.0
47	30.463834383224686	35.0	27.0	38.0	8.0	40.0
48	30.19360629519404	35.0	26.0	38.0	8.0	40.0
49	30.01342950990894	35.0	26.0	38.0	8.0	40.0
50	30.18366916998745	35.0	27.0	38.0	8.0	40.0
51	28.528726867031235	33.0	23.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	20.0
10	18.0
11	19.0
12	37.0
13	44.0
14	74.0
15	159.0
16	338.0
17	667.0
18	1155.0
19	1745.0
20	2644.0
21	3671.0
22	4952.0
23	6550.0
24	8772.0
25	11728.0
26	15067.0
27	16794.0
28	17193.0
29	18075.0
30	20813.0
31	24229.0
32	28351.0
33	32455.0
34	36439.0
35	37406.0
36	28927.0
37	15379.0
38	3235.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.53060806660752	2.879362884585142	37.16259320691118	34.427435841896155
2	34.192434254448564	13.320910690495195	37.78392316160217	14.702731893454079
3	13.986748521596706	13.278183129141086	57.53503511671448	15.20003323254772
4	13.103118815259673	3.2710321969977953	66.17787127728704	17.447977710455493
5	20.87686450912263	4.138935787003107	56.65615291719458	18.328046786679682
6	15.682498612837852	12.49276747008329	59.098745174604396	12.72598874247446
7	58.26021678303004	1.4702435174278008	37.15814241927013	3.111397280272032
8	58.758111560475825	9.490266127429017	27.773805037698175	3.9778172743969926
9	54.31236814541614	3.4380850931253133	27.78537708556491	14.464169675893643
10	28.740812832510926	22.386274957791695	34.286790952438885	14.586121257258494
11	23.683234476394503	18.548212415323764	42.81628038775262	14.952272720529109
12	20.151386123631013	15.729380242656942	45.33512947341248	18.784104160299567
13	18.023909631207736	16.880947364985357	51.19295944739021	13.9021835564167
14	16.300267937415992	19.53183648399645	44.08505158462876	20.0828439939588
15	13.528020675392188	17.517409997655918	51.77423231331171	17.18033701364018
16	15.184307116216	16.942071515255815	44.00315709203338	23.870464276494797
17	15.580427216269706	17.852109228263092	47.7079927244458	18.859470831021397
18	15.19884635584344	16.94770917960115	47.117521564066124	20.73592290048929
19	16.33023657419908	20.11845029508722	42.97799233871088	20.573320792002825
20	18.33754180031393	18.578477771282927	48.41448108266893	14.669499345734216
21	19.06628409674232	22.285687157103904	42.484251629730075	16.163777116423702
22	16.472068340360632	17.09132126081912	44.95977971568369	21.47683068313656
23	16.567908634231305	22.99039520027061	43.15246321424014	17.28923295125794
24	17.48862823757711	17.99928193959391	43.671128334010845	20.840961488818138
25	15.5068408606043	23.128666336319316	41.726430854046804	19.63806194902958
26	15.818395995478	18.70962764710595	45.01259572902418	20.45938062839187
27	16.659594859636996	19.82232455736917	43.21388408368668	20.30419649930716
28	13.895655734543155	19.80748859856566	47.874155463045106	18.422700203846073
29	18.27730780757168	16.81092163943279	43.435830027387176	21.475940525608348
30	15.573009236867952	21.10385467881633	46.19976915248102	17.123366931834706
31	17.03642821324614	18.699539195119563	42.31690201442649	21.947130577207812
32	18.625656120278087	21.443894854592767	40.89146309258528	19.038985932543863
33	16.65247359941131	19.033644987374597	41.20628213839576	23.107599274818334
34	18.04705372694121	20.425554642319867	38.54085377975722	22.986537850981694
35	16.045089445995625	20.355528916767305	42.67652565582356	20.92285598141351
36	18.843744714689674	20.683997044677007	38.95240327696658	21.519854963666738
37	16.54713829190639	22.45570724499212	40.4383729107261	20.558781552375386
38	18.298374869072664	21.673852216047166	40.83508644913195	19.19268646574822
39	18.545838661915205	21.913007871959742	35.65882042258745	23.882333043537603
40	17.668736777451716	21.374462567392342	42.35280503473098	18.60399562042496
41	19.940418789445104	22.585670244110865	35.69976766888514	21.77414329755889
42	16.910322563416305	21.92517335817862	40.94635614015827	20.218147938246805
43	20.724350852622553	21.10800874728131	35.34281450007269	22.82482590002344
44	19.922318919704825	21.51006323085642	36.83679555158611	21.730822297852644
45	17.020998816090486	21.515997614377824	38.07263091991846	23.390372649613226
46	20.462941258504713	24.101608514653478	34.51378112213258	20.92166910470923
47	15.846881036380738	21.191386835757033	43.00499378373326	19.956738344128965
48	19.435699470949707	22.163438856562983	35.04194125553752	23.358920416949786
49	16.517763093475445	20.50596553903489	40.33630151415796	22.639969853331714
50	19.768321667324397	19.937748316860475	37.66731252540658	22.626617490408552
51	18.609336565594226	20.151089404454943	36.27866678139809	24.96090724855275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	313.5
2	568.0
3	11621.0
4	22674.0
5	15961.5
6	9249.0
7	8851.5
8	8454.0
9	8060.5
10	7667.0
11	7146.0
12	6625.0
13	6059.0
14	5493.0
15	4990.0
16	4487.0
17	4062.5
18	3638.0
19	3311.5
20	2985.0
21	2784.5
22	2584.0
23	2506.0
24	2428.0
25	2454.5
26	2855.0
27	3229.0
28	3529.0
29	3829.0
30	4140.5
31	4452.0
32	5166.0
33	5880.0
34	6550.0
35	7220.0
36	7801.0
37	8382.0
38	9055.0
39	9728.0
40	11008.0
41	12288.0
42	13062.0
43	13836.0
44	15014.5
45	16193.0
46	28493.0
47	40793.0
48	33748.0
49	26703.0
50	24722.5
51	22742.0
52	19602.5
53	16463.0
54	14924.5
55	13386.0
56	12393.0
57	11400.0
58	10698.0
59	9996.0
60	9416.5
61	8837.0
62	7838.0
63	6839.0
64	5926.5
65	5014.0
66	4249.5
67	3485.0
68	2938.5
69	2392.0
70	2027.5
71	1663.0
72	1367.0
73	1071.0
74	945.0
75	659.5
76	500.0
77	374.0
78	248.0
79	195.0
80	142.0
81	100.0
82	58.0
83	44.0
84	30.0
85	19.0
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.04929984515871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14848325570617	34.86435271220124
2	6.93806761065124	6.112340410551206
3	3.2121753403315196	4.244822241644634
4	2.1114686938851843	3.7203487044245644
5	1.6697628711610597	3.6775942691043313
6	1.2655743135713202	3.34485974489004
7	1.0430116888051808	3.2160754235529345
8	0.8147454512043184	2.8711173342062515
9	0.6966888409480605	2.761979008832748
>10	3.0198858352898807	19.287001015332375
>50	0.04178035901889293	1.2339701391527746
>100	0.030821576325412818	2.6474542716541807
>500	0.002054771755027521	0.6785327244387752
>1k	0.0034246195917125356	2.8396398410038914
>5k	0.0013698478366850142	4.092013935777282
>10k+	6.849239183425071E-4	4.407898223232772
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14610	4.335067162385504	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	8120	2.409359709689958	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	5443	1.6150424753500543	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	4063	1.2055700123731896	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	1616	0.4794981885294301	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT	1565	0.4643655105498503	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	1097	0.3255009361490005	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	1071	0.3177862375711755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTA	905	0.26853085434352364	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	687	0.2038460739602218	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTTCTCTGTCGTATGCCG	657	0.19494449867811608	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	352	0.10444514997670755	TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTTTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	346	0.10266483492028639	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGT	338	0.10029108151172485	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12343517724519983	0.0
2	0.0	0.0	0.0	0.855144665434293	0.0
3	0.0	0.0	0.0	1.040594150478163	0.0
4	0.0	0.0	0.0	1.6459012696613544	0.0
5	0.0	0.0	0.0	3.302781148837306	0.0
6	0.0	0.0	0.0	3.595049537266445	0.0
7	0.0	0.0	0.0	4.02084155492717	0.0
8	0.0	0.0	0.0	4.593806283918711	0.0
9	0.0	0.0	0.0	4.756705111581246	0.0
10	0.0	0.0	0.0	7.900741501221	0.0
11	0.0	0.0	0.0	8.420000059343835	0.0
12	0.0	0.0	0.0	10.694352543921855	0.0
13	0.0	0.0	0.0	10.937958987475483	0.0
14	0.0	0.0	0.0	11.081867787869527	0.0
15	0.0	0.0	0.0	11.555134873701483	0.0
16	0.0	0.0	0.0	11.848590138834902	0.0
17	0.0	0.0	0.0	12.142638842320462	0.0
18	0.0	0.0	0.0	12.405532032318654	0.0
19	0.0	0.0	0.0	13.168100314819046	0.0
20	0.0	0.0	0.0	13.434850854106148	0.0
21	0.0	0.0	0.0	13.71525047549248	0.0
22	0.0	0.0	0.0	14.167747218999523	0.0
23	0.0	0.0	0.0	14.468323744358626	0.0
24	0.0	0.0	0.0	14.697687667460885	0.0
25	0.0	0.0	0.0	14.897676392132194	0.0
26	0.0	0.0	0.0	15.084312753880345	0.0
27	0.0	0.0	0.0	15.286378512784145	0.0
28	0.0	0.0	0.0	15.482509888166543	0.0
29	0.0	0.0	0.0	15.673597037555746	0.0
30	0.0	0.0	0.0	15.884861090917722	0.0
31	0.0	0.0	0.0	16.08336621970868	0.0
32	0.0	0.0	0.0	16.2842451019082	0.0
33	0.0	0.0	0.0	16.49847634703088	0.0
34	0.0	0.0	0.0	16.688376619715804	0.0
35	0.0	0.0	0.0	16.893409570380307	0.0
36	0.0	0.0	0.0	17.088650788234492	0.0
37	0.0	0.0	0.0	17.293980458075065	0.0
38	0.0	0.0	0.0	17.509101860725952	0.0
39	0.0	0.0	0.0	17.7236298250247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTT	40	6.7902874E-9	45.0	23
CCGAGCT	20	7.0261833E-4	45.0	27
CGAATGT	20	7.0261833E-4	45.0	25
ACATACG	45	3.8380676E-10	45.0	17
CGTTTTT	7630	0.0	44.174313	1
ACGGCTG	655	0.0	43.969467	8
TGATACC	985	0.0	43.401012	4
CGATGAA	125	0.0	43.2	19
CACCGGT	100	0.0	42.75	16
TACGGCT	670	0.0	42.649254	7
GATACCT	1015	0.0	42.118225	5
CGGCTGT	625	0.0	41.760002	9
GAATGAT	3045	0.0	41.674877	1
AATGATA	2830	0.0	41.660778	2
TGGGACG	65	0.0	41.538464	6
ATGATAC	2850	0.0	41.526318	3
ACCACCG	365	0.0	41.30137	14
AACATAC	55	6.002665E-11	40.909092	16
GATACGG	1870	0.0	40.78877	5
ATACGGC	1855	0.0	40.75472	6
>>END_MODULE