##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933418.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	737099
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.544788420551377	31.0	28.0	33.0	25.0	34.0
2	28.855895883727968	31.0	28.0	33.0	16.0	34.0
3	28.828842529972228	31.0	28.0	34.0	16.0	34.0
4	33.24995421239209	35.0	32.0	37.0	28.0	37.0
5	34.29695875316613	35.0	33.0	37.0	30.0	37.0
6	33.757468128433224	35.0	33.0	37.0	28.0	37.0
7	33.70616158752081	35.0	33.0	37.0	29.0	37.0
8	33.61510597626642	35.0	33.0	37.0	28.0	37.0
9	34.84517819180327	37.0	34.0	39.0	28.0	39.0
10	34.747508814962444	37.0	33.0	39.0	27.0	39.0
11	35.03976535038034	37.0	34.0	39.0	28.0	39.0
12	35.162020298494504	37.0	34.0	39.0	28.0	39.0
13	35.27759364752903	37.0	34.0	39.0	30.0	39.0
14	36.087503849550735	38.0	34.0	40.0	28.0	41.0
15	36.37816765454844	38.0	34.0	40.0	30.0	41.0
16	36.37039664956811	38.0	34.0	40.0	30.0	41.0
17	35.629399849952314	38.0	33.0	40.0	27.0	41.0
18	35.4376766214579	38.0	34.0	39.0	27.0	40.0
19	34.73891702471445	37.0	33.0	38.0	27.0	40.0
20	33.92979097787407	35.0	32.0	38.0	27.0	40.0
21	34.30708900703976	35.0	33.0	38.0	27.0	40.0
22	34.77517402682679	35.0	33.0	39.0	29.0	40.0
23	35.134394430056204	35.0	34.0	39.0	30.0	40.0
24	34.57883405078558	35.0	33.0	39.0	27.0	40.0
25	33.56689942599298	35.0	33.0	38.0	24.0	40.0
26	33.58665118254129	35.0	32.0	38.0	24.0	40.0
27	34.32012389109197	35.0	33.0	38.0	27.0	40.0
28	34.08951714762875	35.0	33.0	39.0	25.0	40.0
29	34.008232272734055	35.0	33.0	39.0	24.0	40.0
30	33.25441901291414	35.0	33.0	39.0	21.0	40.0
31	33.13775354463919	35.0	33.0	39.0	21.0	40.0
32	32.40407462226919	35.0	31.0	39.0	16.0	40.0
33	31.59011340403392	35.0	30.0	39.0	12.0	40.0
34	30.840196500063087	35.0	26.0	39.0	8.0	40.0
35	30.37382495431414	35.0	23.0	40.0	7.0	41.0
36	29.698121961907425	35.0	21.0	39.0	7.0	40.0
37	29.5970676937562	35.0	21.0	39.0	7.0	40.0
38	29.44952984605867	35.0	20.0	39.0	7.0	40.0
39	29.081347281708428	35.0	18.0	39.0	7.0	40.0
40	28.886822529945096	35.0	18.0	39.0	7.0	40.0
41	28.46387798653912	34.0	18.0	39.0	7.0	40.0
42	28.63657392019254	35.0	18.0	39.0	7.0	40.0
43	28.52597819288861	35.0	18.0	39.0	7.0	40.0
44	28.630684616313413	35.0	18.0	39.0	7.0	40.0
45	28.80994547543817	35.0	18.0	39.0	7.0	40.0
46	28.599802740201792	35.0	18.0	39.0	7.0	40.0
47	28.466783973387564	35.0	18.0	39.0	7.0	40.0
48	28.319985510765854	34.0	18.0	38.0	7.0	40.0
49	28.507976540464714	35.0	18.0	39.0	7.0	40.0
50	28.415090781563944	35.0	18.0	39.0	7.0	40.0
51	27.28821637256325	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	14.0
9	17.0
10	34.0
11	46.0
12	62.0
13	79.0
14	134.0
15	212.0
16	389.0
17	813.0
18	1732.0
19	3132.0
20	4993.0
21	7321.0
22	10773.0
23	15709.0
24	23333.0
25	33450.0
26	41589.0
27	41391.0
28	35877.0
29	31576.0
30	30801.0
31	34601.0
32	39130.0
33	46698.0
34	53117.0
35	63285.0
36	71711.0
37	76249.0
38	59850.0
39	8976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.69899023062031	2.2834110479053695	31.613663836201106	40.403934885273216
2	40.60648569595129	15.791908549597816	30.672948952583027	12.928656801867863
3	11.31896800836794	15.983334667392032	59.50435423192813	13.193343092311888
4	10.42383723217641	2.6020927989320297	71.99521366872021	14.978856300171348
5	20.476760923566577	3.160226780934447	60.17848348729275	16.184528808206224
6	12.760429738746085	13.97641293774649	63.49201396284624	9.771143360661187
7	49.88556489698127	1.2087928487218136	46.378030630892184	2.527611623404726
8	49.05067026274625	10.227255768899429	37.66780310378932	3.0542708645650043
9	46.02787413902339	2.7430507977897136	36.71433552345072	14.514739539736182
10	26.932474470864836	16.355740545028553	44.30788808558959	12.403896898517024
11	23.135155521849846	15.845090008262119	47.96926871424327	13.050485755644765
12	17.07613224275165	13.896776416736422	51.80715209218844	17.219939248323495
13	16.79665825079128	14.682559601898795	57.25567393253823	11.265108214771692
14	15.048860465147829	20.150753155274938	49.94851437866556	14.851872000911682
15	11.843727911718778	15.199450820039099	59.03169045135049	13.925130816891626
16	13.794890509958634	16.91021151839848	49.5634914712949	19.731406500347987
17	14.60902809527621	17.011147756271548	53.622919038012526	14.75690511043971
18	14.484078800812375	16.295233069099265	52.33435400129426	16.886334128794097
19	14.754598771671104	18.460342504873836	49.220389662718304	17.564669060736755
20	16.267828337848783	18.074776929557633	54.166400985484984	11.490993747108599
21	16.18208680245123	21.441081862816258	50.27235147517498	12.104479859557536
22	14.144232999909104	14.223598186946393	53.66985981530297	17.96230899784154
23	14.57565401662463	20.766002938546926	51.728193906110306	12.930149138718136
24	15.702232671594995	17.1430160670412	49.17941823282897	17.975333028534838
25	13.460200054538129	24.53591715631143	47.571628777138486	14.432254012011954
26	13.909257779484166	16.7076607077204	51.399744132063674	17.983337380731758
27	17.057138864657258	18.36551128138825	48.22907099317731	16.34827886077718
28	12.811033524669007	18.626805897172563	52.41290518641323	16.149255391745207
29	17.078709915493036	18.786485940151863	47.78109860412238	16.353705540232724
30	17.460069814231197	19.941690329250207	49.46241956643544	13.13582029008315
31	19.58420782011643	17.21030689228991	45.45196778180407	17.75351750578959
32	18.448268143085258	21.152653849754238	44.084037558048514	16.315040449111994
33	17.36564559170478	18.50958962093287	43.75043243851911	20.374332348843236
34	18.071249587911527	26.635228103687563	40.24452617626669	15.048996132134217
35	19.09458566624022	24.472560673667985	38.333656672984226	18.099196987107565
36	22.871961568256097	24.469168999008275	36.30448555757096	16.354383875164665
37	19.741445857340736	23.926229719481373	37.948498098627184	18.383826324550707
38	21.87399521638206	23.374336418852828	37.41858284979358	17.33308551497153
39	20.345570947728866	25.332960701344053	34.91688362078907	19.404584730138012
40	18.85133475964558	25.076007429124175	39.412616215732214	16.660041595498026
41	21.58393919948338	27.16134467690229	33.95039200975717	17.304324113857163
42	17.801136618011963	25.891094683346473	38.7941104247869	17.513658273854666
43	22.47147262443715	22.76247831024055	35.287661494588924	19.478387570733375
44	21.241244391865948	23.296327901679422	35.367705016558155	20.09472268989647
45	18.551782053699707	22.840758161386734	35.48953397033506	23.117925814578502
46	22.525603752006177	24.395094824440136	33.68190704369427	19.397394379859424
47	16.68486865400713	21.901399947632544	42.85950733890563	18.5542240594547
48	19.78282428818924	25.313288988317716	33.65748698614433	21.246399737348714
49	17.72095742905634	22.87413224003831	39.261347525909	20.143562804996343
50	19.726522488838	21.592486219625858	36.93791471701901	21.74307657451713
51	18.735068152310614	22.398348118773733	34.74607888492591	24.12050484398975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	1109.0
2	2145.0
3	24413.5
4	46682.0
5	35442.0
6	24202.0
7	23297.0
8	22392.0
9	21974.5
10	21557.0
11	20875.0
12	20193.0
13	19225.5
14	18258.0
15	17330.0
16	16402.0
17	15361.0
18	14320.0
19	13420.5
20	12521.0
21	11708.5
22	10896.0
23	10356.0
24	9816.0
25	9547.0
26	9227.5
27	9177.0
28	9805.0
29	10433.0
30	11296.5
31	12160.0
32	12984.5
33	13809.0
34	14943.5
35	16078.0
36	17050.0
37	18022.0
38	19266.5
39	20511.0
40	21975.0
41	23439.0
42	24442.5
43	25446.0
44	27973.0
45	30500.0
46	68372.0
47	106244.0
48	75171.5
49	44099.0
50	40618.5
51	37138.0
52	32456.0
53	27774.0
54	26491.5
55	25209.0
56	23502.0
57	21795.0
58	20170.5
59	18546.0
60	16430.0
61	14314.0
62	12372.5
63	10431.0
64	8836.0
65	7241.0
66	6356.5
67	5472.0
68	4599.5
69	3727.0
70	3072.0
71	2417.0
72	2137.5
73	1858.0
74	1532.0
75	944.0
76	682.0
77	496.0
78	310.0
79	236.0
80	162.0
81	130.5
82	99.0
83	67.0
84	35.0
85	25.0
86	15.0
87	11.0
88	7.0
89	5.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	737099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.02475697315416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26717678627806	39.15618752215389
2	5.633637392481259	5.2984085851261025
3	2.5613090268677343	3.6133480356480354
4	1.730630626252528	3.255299384392909
5	1.3116027256711693	3.083889971000666
6	1.0465896687228546	2.9529374893383697
7	0.8542979113626576	2.8121206165151515
8	0.675393883450561	2.5408186584333947
9	0.5452159745029703	2.3074783828995233
>10	2.279196495049308	15.818697025397649
>50	0.057731653097437656	1.8998205073813377
>100	0.03194288450964195	2.848903230845522
>500	0.0017583239179613697	0.5477865013596921
>1k	0.0023444318906151594	3.2660478194275977
>5k	0.0	0.0
>10k+	0.0011722159453075797	10.59825627008012
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22906	3.1075879902156966	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	18789	2.5490470072541136	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	18307	2.4836555198148416	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	16904	2.2933147379117322	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCT	4240	0.5752280222873725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	3604	0.4889438189442667	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGCTAAGT	3562	0.4832458055159483	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	3350	0.4544844044015797	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT	3244	0.4401037038443954	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA	3087	0.418803986981396	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	1362	0.18477843546117959	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGT	1251	0.1697193999720526	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATG	822	0.1115182628113727	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTAT	773	0.10487058047833465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06281381469788998	0.0
2	0.0	0.0	0.0	0.5874380510623404	0.0
3	0.0	0.0	0.0	0.6768425950923824	0.0
4	0.0	0.0	0.0	1.3682015577283377	0.0
5	0.0	0.0	0.0	4.23416664518606	0.0
6	0.0	0.0	0.0	4.511198631391442	0.0
7	0.0	0.0	0.0	5.071774619148853	0.0
8	0.0	0.0	0.0	5.69638542448165	0.0
9	0.0	0.0	0.0	5.795422324545278	0.0
10	0.0	0.0	0.0	8.820253453063971	0.0
11	0.0	0.0	0.0	8.977762824261056	0.0
12	0.0	0.0	0.0	11.742656006859322	0.0
13	0.0	0.0	0.0	11.952261500829604	0.0
14	0.0	0.0	0.0	12.11248421175446	0.0
15	0.0	0.0	0.0	12.658679498954687	0.0
16	0.0	0.0	0.0	12.929606470772583	0.0
17	0.0	0.0	0.0	13.054962766195585	0.0
18	0.0	0.0	0.0	13.164310357224743	0.0
19	0.0	0.0	0.0	13.86285967013929	0.0
20	0.0	0.0	0.0	14.011822021193897	0.0
21	0.0	0.0	0.0	14.124832620855543	0.0
22	0.0	0.0	0.0	14.351803489083556	0.0
23	0.0	0.0	0.0	14.46413575381326	0.0
24	0.0	0.0	0.0	14.570770005114646	0.0
25	0.0	0.0	0.0	14.656647207498585	0.0
26	0.0	0.0	0.0	14.768165470309958	0.0
27	0.0	0.0	0.0	14.926217509452597	0.0
28	0.0	0.0	0.0	15.017792725264856	0.0
29	0.0	0.0	0.0	15.129039654103451	0.0
30	0.0	0.0	0.0	15.25670228829506	0.0
31	0.0	0.0	0.0	15.372290560698088	0.0
32	0.0	0.0	0.0	15.495340517352487	0.0
33	0.0	0.0	0.0	15.622596150584927	0.0
34	0.0	0.0	0.0	15.794486222339197	0.0
35	0.0	0.0	0.0	15.941549235584365	0.0
36	0.0	0.0	0.0	16.079386893755114	0.0
37	0.0	0.0	0.0	16.226992574945836	0.0
38	0.0	0.0	0.0	16.402681322318983	0.0
39	0.0	0.0	0.0	16.65339391316499	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	20	7.031639E-4	45.0	19
ACGATAC	25	3.8894395E-5	45.0	3
CAAGACG	25	3.8894395E-5	45.0	38
CGATACG	20	7.031639E-4	45.0	4
CGTTTTT	21085	0.0	44.5198	1
TACGGCT	1980	0.0	42.5	7
CCGATGA	605	0.0	42.39669	18
CGGCTGT	2030	0.0	42.00739	9
CGATGAA	600	0.0	42.000004	19
ACGGCTG	1995	0.0	41.842106	8
GATACCT	2290	0.0	41.46288	5
TGATACC	2320	0.0	40.926723	4
ATACCTG	2345	0.0	40.682304	6
CGGTAGT	100	0.0	40.5	12
TGATGCG	50	1.0804797E-9	40.5	4
GAATGAC	410	0.0	40.060974	1
CACCGGT	130	0.0	39.807693	16
ATGATAC	7365	0.0	39.68432	3
GAATGAT	7725	0.0	39.6699	1
ATACGGC	4940	0.0	39.352226	6
>>END_MODULE