##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933417.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486561
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.766068386081088	31.0	28.0	31.0	16.0	33.0
2	29.075330739619492	31.0	30.0	33.0	16.0	34.0
3	29.222249625432372	31.0	30.0	33.0	16.0	34.0
4	32.26742998308537	35.0	32.0	37.0	19.0	37.0
5	33.73077373648936	35.0	33.0	37.0	28.0	37.0
6	32.91491097724643	35.0	33.0	37.0	23.0	37.0
7	32.210374444314276	35.0	32.0	35.0	25.0	37.0
8	33.06184836022616	35.0	32.0	36.0	28.0	37.0
9	33.082182912317265	35.0	31.0	37.0	25.0	39.0
10	31.955308789648164	35.0	28.0	39.0	15.0	39.0
11	32.52923477220739	37.0	30.0	39.0	17.0	39.0
12	33.39035393301148	35.0	31.0	39.0	23.0	39.0
13	33.477013159706594	35.0	32.0	39.0	25.0	39.0
14	33.668335522164746	37.0	32.0	40.0	20.0	41.0
15	34.32474653743313	37.0	32.0	40.0	25.0	41.0
16	34.60541021577973	37.0	32.0	40.0	25.0	41.0
17	32.630963024163464	36.0	29.0	40.0	18.0	41.0
18	33.28269836670017	36.0	31.0	39.0	23.0	39.0
19	33.05204075131381	36.0	31.0	37.0	22.0	39.0
20	32.5455348866843	35.0	31.0	36.0	24.0	39.0
21	32.83938498975463	35.0	31.0	37.0	24.0	39.0
22	32.92366013716677	35.0	31.0	37.0	25.0	39.0
23	34.00195453396388	35.0	33.0	38.0	27.0	40.0
24	32.180119245068965	35.0	31.0	37.0	21.0	39.0
25	31.363448776206887	34.0	29.0	37.0	18.0	39.0
26	30.529467014413402	33.0	25.0	37.0	18.0	39.0
27	32.28662387655402	34.0	30.0	37.0	22.0	39.0
28	32.40131042150933	35.0	31.0	37.0	21.0	39.0
29	32.493224898830775	35.0	31.0	38.0	21.0	40.0
30	31.50526449920976	35.0	30.0	38.0	18.0	40.0
31	31.78312071867659	35.0	30.0	37.0	20.0	40.0
32	31.44391967297009	35.0	30.0	37.0	17.0	40.0
33	31.104469943131487	35.0	30.0	38.0	15.0	40.0
34	29.990317760774087	35.0	25.0	38.0	8.0	40.0
35	29.362604072254044	35.0	23.0	38.0	7.0	40.0
36	28.745795491212817	34.0	21.0	38.0	7.0	40.0
37	28.79679012497919	34.0	21.0	38.0	7.0	40.0
38	28.81997529600605	34.0	21.0	38.0	7.0	40.0
39	28.3558032805753	34.0	20.0	38.0	7.0	40.0
40	28.193318001237255	33.0	18.0	38.0	7.0	40.0
41	27.755391410326762	33.0	18.0	38.0	7.0	40.0
42	28.066639126440467	33.0	18.0	38.0	7.0	40.0
43	28.09922496870896	34.0	18.0	38.0	7.0	40.0
44	28.084552604914904	34.0	18.0	38.0	7.0	40.0
45	28.369390477247457	34.0	20.0	38.0	7.0	40.0
46	28.14282279097585	34.0	18.0	38.0	7.0	40.0
47	28.150324419754153	34.0	18.0	38.0	7.0	40.0
48	27.876794482089604	34.0	18.0	37.0	7.0	40.0
49	27.673381138233438	33.0	18.0	37.0	7.0	40.0
50	27.833891331199993	34.0	18.0	38.0	7.0	40.0
51	26.322596755596933	31.0	15.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	18.0
10	25.0
11	33.0
12	42.0
13	57.0
14	100.0
15	258.0
16	589.0
17	1104.0
18	1739.0
19	3077.0
20	4514.0
21	6504.0
22	9336.0
23	12730.0
24	17436.0
25	23715.0
26	28985.0
27	28560.0
28	27178.0
29	26635.0
30	28560.0
31	32092.0
32	36204.0
33	41015.0
34	46055.0
35	48419.0
36	36961.0
37	20188.0
38	4349.0
39	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.733063274697315	2.283166961593716	34.660196768750474	42.3235729949585
2	43.653930339669635	10.252979585293518	33.0630691732383	13.030020901798542
3	12.279035927663747	10.282575052254495	63.91428823929579	13.524100780785966
4	11.240933819192248	2.7809462739512623	70.36425032010375	15.61386958675274
5	17.414877065773872	3.2892073141908207	63.35033017442828	15.945585445607025
6	13.384344409025795	9.690665713034953	65.89101880339773	11.033971074541526
7	51.311551891746355	1.2886359572592132	44.76745978407641	2.6323523669180227
8	52.492493233119795	6.847240119943851	37.62734785566455	3.0329187912718036
9	48.46483791343737	2.801293157486934	37.63228043349138	11.101588495584314
10	25.43031603437185	18.019323373636606	44.495140383220196	12.055220208771358
11	21.654838756086082	16.500500451125347	49.480948945764254	12.36371184702432
12	17.48619392018678	14.565696798551468	52.54161348731196	15.406495793949782
13	16.597302290976877	15.073135742486555	56.5624865124825	11.767075454054066
14	14.665992547696998	17.36329052266828	52.439468021481375	15.531248908153344
15	12.26793762755338	15.480689985428342	58.25004470148656	14.001327685531722
16	14.295638162532551	15.504530778258019	51.641418033915585	18.55841302529385
17	14.957014639479944	15.642026385180893	54.32289065502578	15.078068320313385
18	14.842332205006157	15.201588290060238	53.56943939197757	16.386640112956034
19	15.375050610303745	17.409327915718688	50.750471163944496	16.46515031003307
20	16.516120280910307	17.490509925785254	54.069684993248536	11.923684800055904
21	16.705613479091006	19.076539221187065	51.20570699254564	13.012140307176285
22	14.750462942981454	14.794239571194566	53.84093669652932	16.614360789294665
23	14.766082772766415	19.38215352237438	51.92093077743593	13.930832927423284
24	14.717990138954828	16.714039966211843	51.14363872155804	17.42433117327529
25	14.121148221908456	20.51089174841387	49.57549002077848	15.792470008899192
26	13.963100207373794	17.427002986264824	52.009922702394974	16.59997410396641
27	15.31791491714297	17.85264334790499	50.653669324092974	16.17577241085907
28	13.10154328028757	17.963626349008656	53.26300299448579	15.671827376217987
29	16.359099886756233	17.482905534968893	50.1819915694024	15.976003008872475
30	16.24112906706456	18.4809304485974	51.198102601729275	14.079837882608759
31	17.596971397214325	17.313759220323867	48.34337318445169	16.745896198010115
32	17.307799022116445	20.231790053045763	46.99842363033618	15.461987294501615
33	16.818446196879734	18.430782574024633	46.01190806497027	18.73886316412536
34	17.0718573827331	24.643569870992536	42.80778771829226	15.476785027982103
35	17.935675074656622	23.72569934705001	40.59902047225322	17.739605106040145
36	21.208851510910247	22.96238292834814	38.9990155396754	16.829750021066218
37	19.247740776593275	23.49140190027561	39.33607502450875	17.92478229862237
38	20.404635801060916	22.891477122087466	39.917091587694046	16.786795489157576
39	18.853134550446914	24.356247212579717	37.987426037023106	18.803192199950264
40	18.508881722949436	24.083516763571268	40.71555262341207	16.692048890067227
41	20.352021637574733	25.223353289721125	37.22513723870183	17.199487834002312
42	17.981712467707027	24.607192109519673	39.9499754398729	17.461119982900396
43	20.666678998111234	22.48001792169944	38.290985097449244	18.562317982740087
44	20.43834174954425	22.88531139980393	37.60391811098711	19.07242873966471
45	18.514430873004617	22.565310413288366	37.835132696619745	21.085126017087273
46	20.03756980111435	23.682539291065254	36.686253111120706	19.593637796699696
47	17.0052675820709	21.941955890422786	42.694543952351296	18.358232575155018
48	19.169641627668472	24.174769453367613	36.55923923207985	20.09634968688407
49	17.53510865030284	22.571270611495784	39.69985263923742	20.19376809896395
50	18.7037185471092	21.54077289384065	38.58878948374407	21.166719075306077
51	18.330897873031336	22.057254897124924	37.26624205392541	22.345605175918333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	467.5
2	847.0
3	21758.0
4	42669.0
5	29730.0
6	16791.0
7	15982.5
8	15174.0
9	14920.5
10	14667.0
11	13972.0
12	13277.0
13	12633.5
14	11990.0
15	11276.5
16	10563.0
17	9774.5
18	8986.0
19	8273.0
20	7560.0
21	6991.5
22	6423.0
23	6093.5
24	5764.0
25	5630.0
26	5599.0
27	5702.0
28	6119.5
29	6537.0
30	7120.5
31	7704.0
32	8131.0
33	8558.0
34	9460.5
35	10363.0
36	11137.0
37	11911.0
38	12728.0
39	13545.0
40	14743.5
41	15942.0
42	16836.0
43	17730.0
44	19290.5
45	20851.0
46	33767.5
47	46684.0
48	39244.5
49	31805.0
50	29776.0
51	27747.0
52	24381.0
53	21015.0
54	19343.0
55	17671.0
56	16120.0
57	14569.0
58	13408.5
59	12248.0
60	11092.5
61	9937.0
62	8754.5
63	7572.0
64	6505.0
65	5438.0
66	4846.0
67	4254.0
68	3565.5
69	2877.0
70	2423.5
71	1970.0
72	1716.5
73	1463.0
74	1264.5
75	809.5
76	553.0
77	419.5
78	286.0
79	210.5
80	135.0
81	109.5
82	84.0
83	58.5
84	33.0
85	19.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.780950181891946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.10784382454244	40.87406482972112
2	6.655379819876346	6.626222625096667
3	2.989103604844755	4.464012529238711
4	1.8809576810519355	3.745434424587736
5	1.3469271797616533	3.3525657417175543
6	1.0502463015363108	3.136935528929721
7	0.808921767171242	2.818822593481971
8	0.6365221344555095	2.5349341332000996
9	0.511012371861562	2.289481328337377
>10	1.9325024653721248	14.037392201163904
>50	0.04238993647035394	1.4761032618671937
>100	0.03315648496046702	3.1745099731143624
>500	0.002098511706358672	0.7623921871721935
>1k	0.0016788093650869378	1.9123528882398155
>5k	8.394046825434689E-4	2.9377902285086908
>10k+	4.1970234127173444E-4	5.856985525622938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28033	5.761456425813002	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	8098	1.6643339684027287	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	5963	1.2255400658910187	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	4798	0.9861045172136689	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGCTAAGT	1572	0.32308384765733383	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	1401	0.2879392306411735	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCT	1382	0.2840342731949334	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	958	0.19689206492094516	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA	809	0.16626897757937853	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT	643	0.13215198094380767	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGGCTAAGTCGTATGCCG	642	0.13194645686768977	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	597	0.1226978734423844	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0774825766964471	0.0
2	0.0	0.0	0.0	0.511960473609681	0.0
3	0.0	0.0	0.0	0.6467842675430213	0.0
4	0.0	0.0	0.0	1.1462077725095106	0.0
5	0.0	0.0	0.0	2.4535464206954525	0.0
6	0.0	0.0	0.0	2.7248382011710763	0.0
7	0.0	0.0	0.0	3.0405231820881657	0.0
8	0.0	0.0	0.0	3.46225858628209	0.0
9	0.0	0.0	0.0	3.6240060341868747	0.0
10	0.0	0.0	0.0	5.794134753915747	0.0
11	0.0	0.0	0.0	6.2536865881153645	0.0
12	0.0	0.0	0.0	8.033114039144115	0.0
13	0.0	0.0	0.0	8.236171826348597	0.0
14	0.0	0.0	0.0	8.361541512780514	0.0
15	0.0	0.0	0.0	8.698806521689983	0.0
16	0.0	0.0	0.0	8.912757084928714	0.0
17	0.0	0.0	0.0	9.120541925883908	0.0
18	0.0	0.0	0.0	9.334081440970403	0.0
19	0.0	0.0	0.0	9.90995990225275	0.0
20	0.0	0.0	0.0	10.11897788766465	0.0
21	0.0	0.0	0.0	10.358413436342	0.0
22	0.0	0.0	0.0	10.692801108185819	0.0
23	0.0	0.0	0.0	10.940663143984002	0.0
24	0.0	0.0	0.0	11.136527588524357	0.0
25	0.0	0.0	0.0	11.302591042027618	0.0
26	0.0	0.0	0.0	11.463310869551814	0.0
27	0.0	0.0	0.0	11.657531121483226	0.0
28	0.0	0.0	0.0	11.827088484280491	0.0
29	0.0	0.0	0.0	12.001783948980703	0.0
30	0.0	0.0	0.0	12.202786495424007	0.0
31	0.0	0.0	0.0	12.376659863819746	0.0
32	0.0	0.0	0.0	12.556904478575143	0.0
33	0.0	0.0	0.0	12.729750226590294	0.0
34	0.0	0.0	0.0	12.936712971241016	0.0
35	0.0	0.0	0.0	13.150252486327512	0.0
36	0.0	0.0	0.0	13.339745684508213	0.0
37	0.0	0.0	0.0	13.540131658723162	0.0
38	0.0	0.0	0.0	13.753876697885774	0.0
39	0.0	0.0	0.0	14.109433349569736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGG	50	2.1827873E-11	45.0	3
TATCGGG	25	3.8874776E-5	45.0	3
CGTTTTT	15925	0.0	44.604397	1
TGATACC	1090	0.0	41.90367	4
ACGGCTG	815	0.0	41.68712	8
CGGCTGT	785	0.0	41.560513	9
TACGGCT	820	0.0	41.432926	7
CGACCAA	60	3.6379788E-12	41.249996	29
ACGACCA	60	3.6379788E-12	41.249996	28
GAATGAT	3170	0.0	41.16719	1
GATACCT	1110	0.0	41.148647	5
CCGATGA	170	0.0	41.029415	18
ATACGGC	1880	0.0	40.93085	6
AATGATA	2975	0.0	40.613445	2
ATGATAC	3025	0.0	40.46281	3
GCGACCA	740	0.0	40.43919	11
CGACCAC	735	0.0	40.408165	12
TGATACG	1925	0.0	40.207794	4
GATACGG	1915	0.0	40.182766	5
GTTTTTT	17915	0.0	40.06419	2
>>END_MODULE