##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933416.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683128
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.45068859715895	31.0	28.0	33.0	25.0	34.0
2	28.672213699335995	31.0	28.0	33.0	16.0	34.0
3	28.651561639985477	31.0	27.0	34.0	16.0	34.0
4	33.17998383904627	35.0	32.0	37.0	28.0	37.0
5	34.27664215198323	35.0	33.0	37.0	30.0	37.0
6	33.693331264418966	35.0	33.0	37.0	28.0	37.0
7	33.72255419189376	35.0	33.0	37.0	29.0	37.0
8	33.564460833108875	35.0	33.0	37.0	28.0	37.0
9	34.78842618074504	37.0	34.0	39.0	28.0	39.0
10	34.78139235985057	37.0	33.0	39.0	28.0	39.0
11	35.10697409563069	37.0	34.0	39.0	28.0	39.0
12	35.2185666522233	37.0	34.0	39.0	29.0	39.0
13	35.33934928739563	37.0	34.0	39.0	30.0	39.0
14	36.12973264161329	38.0	34.0	40.0	29.0	41.0
15	36.41991691161832	38.0	34.0	40.0	30.0	41.0
16	36.35909375695331	38.0	34.0	40.0	30.0	41.0
17	35.621646309330025	38.0	33.0	40.0	27.0	41.0
18	35.37661463151854	38.0	34.0	39.0	27.0	40.0
19	34.67685411811549	37.0	33.0	38.0	27.0	40.0
20	33.880372346031784	35.0	32.0	38.0	27.0	40.0
21	34.23837113981567	35.0	33.0	38.0	27.0	40.0
22	34.71741167101919	35.0	33.0	39.0	29.0	40.0
23	35.01697778454404	35.0	33.0	39.0	30.0	40.0
24	34.461506481947744	35.0	33.0	38.0	27.0	40.0
25	33.471160602405405	35.0	33.0	38.0	24.0	40.0
26	33.47306946867937	35.0	32.0	38.0	24.0	40.0
27	34.25255442611048	35.0	33.0	38.0	26.0	40.0
28	33.950180639645865	35.0	33.0	39.0	24.0	40.0
29	33.86392740452741	35.0	33.0	39.0	24.0	40.0
30	33.137690154700145	35.0	33.0	39.0	21.0	40.0
31	33.129722394631756	35.0	33.0	39.0	21.0	40.0
32	32.31898560738251	35.0	31.0	39.0	16.0	40.0
33	31.52938834303381	35.0	30.0	39.0	12.0	40.0
34	30.787568362005363	35.0	25.0	39.0	8.0	40.0
35	30.14518216205455	35.0	23.0	39.0	7.0	40.0
36	29.587305746507244	35.0	21.0	39.0	7.0	40.0
37	29.356542258551837	35.0	20.0	39.0	7.0	40.0
38	29.273317738403346	35.0	20.0	39.0	7.0	40.0
39	28.87895240716235	35.0	18.0	39.0	7.0	40.0
40	28.622419224508437	35.0	18.0	39.0	7.0	40.0
41	28.317843800868943	34.0	18.0	39.0	7.0	40.0
42	28.40830415383354	35.0	17.0	39.0	7.0	40.0
43	28.297575271398625	35.0	17.0	39.0	7.0	40.0
44	28.358928341394293	35.0	17.0	39.0	7.0	40.0
45	28.51367825649073	35.0	18.0	39.0	7.0	40.0
46	28.27794791020131	35.0	17.0	39.0	7.0	40.0
47	28.12181758030706	34.0	17.0	39.0	7.0	40.0
48	28.007348549612956	34.0	17.0	38.0	7.0	40.0
49	28.148822768207424	34.0	17.0	39.0	7.0	40.0
50	28.09303088147463	34.0	17.0	39.0	7.0	40.0
51	26.991636999215373	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	21.0
10	38.0
11	42.0
12	80.0
13	86.0
14	119.0
15	211.0
16	407.0
17	829.0
18	1651.0
19	3025.0
20	4827.0
21	7067.0
22	10301.0
23	14967.0
24	21795.0
25	31701.0
26	39711.0
27	39058.0
28	33882.0
29	30356.0
30	30189.0
31	32142.0
32	36316.0
33	42839.0
34	49303.0
35	58105.0
36	65289.0
37	68097.0
38	52913.0
39	7745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.783015774496143	2.3721762246606786	30.969013127847195	40.87579487299598
2	40.90185148317738	15.989975524352683	30.098898010328956	13.009274982140976
3	11.11490086777295	16.183350704406788	59.479775386164825	13.221973041655444
4	9.90766005785153	2.612248363410664	72.6515382183134	14.8285533604244
5	20.28843789158108	3.0676534997833493	60.831352250237146	15.812556358398428
6	12.490338560269818	13.942921385157685	63.68337998149688	9.883360073075616
7	48.91791875021958	1.0835451042849948	47.465043154430795	2.533492991064632
8	48.11118853274935	10.444309119228022	38.38080125540163	3.063701092621002
9	45.00854891030671	2.745312737876357	37.647702919511424	14.598435432305513
10	26.0996475038353	16.855991849258118	44.65707744375871	12.387283203147872
11	21.732383974891967	15.30708739796934	49.856103102200464	13.104425524938227
12	16.537896265414386	13.609748099916851	52.463959902097415	17.38839573257135
13	15.919856893583633	14.542808961131733	58.323623098453005	11.213711046831634
14	14.739258235645442	19.76349966624117	50.59827733601902	14.898964762094366
15	11.625786089868956	14.981526156152288	59.20515628110691	14.187531472871848
16	13.259594102422973	16.759816608307666	49.75465798503355	20.225931304235807
17	13.90954550245342	17.156960335398345	54.22292747479243	14.710566687355811
18	13.930185850967899	15.769079879612605	53.20598775046551	17.094746518953986
19	13.75232752866227	18.78535208628544	49.74968087971801	17.712639505334284
20	15.844175615697203	17.280948811935684	55.028193837758074	11.846681734609033
21	15.488019814734574	21.81099296178754	50.42451780632619	12.27646941715169
22	14.021676757503718	14.339772341347448	53.35866777529248	18.279883125856355
23	14.12107247836423	20.83709055989507	51.99157405347168	13.050262908269023
24	15.627378763569933	16.649441978662857	50.1823962712698	17.540782986497405
25	12.823072689159279	24.438172641144853	48.24674145987282	14.492013209823048
26	14.182407982105843	15.802894918668246	52.19212212059819	17.822574978627724
27	16.151585061657553	18.61188532749353	48.45197386141397	16.78455574943495
28	12.504977105315549	17.954614654940215	52.94659273225516	16.59381550748908
29	18.99146280052933	16.40029394198452	48.47612746073942	16.132115796746728
30	13.654102891405417	22.296846271855348	50.37386844046796	13.675182396271271
31	17.053465821925027	17.01730861566207	46.0389560960757	19.8902694663372
32	17.705027461910504	23.513016594254662	44.81034301038751	13.97161293344732
33	16.077514023726152	18.465353491585766	44.41568783595461	21.041444648733474
34	23.328717312128912	20.8616833155719	40.422731903830616	15.386867468468573
35	15.745219051188064	27.63420618097926	39.01377194317902	17.606802824653652
36	22.325098663793607	21.121371104683163	36.43899825508543	20.114531976437796
37	16.97661346043494	28.65890433418042	38.378751859095225	15.985730346289422
38	18.376058366806806	23.187016196086237	38.30131395580331	20.13561148130365
39	23.344966097129674	24.63608577016313	35.38238807368458	16.636560059022614
40	18.499022145190946	25.759447716972517	39.44355962572168	16.29797051211486
41	23.65091168858545	24.54488763452823	34.15128643533862	17.652914241547705
42	17.542393226452436	25.98283191437037	38.9552177629961	17.519557096181096
43	23.13431743392161	21.71862374254898	35.19969903151386	19.94735979201555
44	20.999578409902682	23.26474687027907	35.40654167301003	20.329133046808213
45	17.559373938705484	22.324513121991778	36.38351816936211	23.732594769940626
46	22.57673525312972	24.771199540935225	34.0207106135307	18.63135459240435
47	16.508619175322924	21.926198311297444	42.94890562237238	18.61627689100725
48	20.247303580002576	24.167798714150205	33.92790223794076	21.656995467906455
49	17.155789251794687	22.512911196730336	39.80864493916221	20.522654612312774
50	19.647123233127616	20.979084446838662	37.42095185675306	21.952840463280673
51	18.987510393366982	21.374617933974307	35.17364242133246	24.464229251326252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	970.5
2	1886.0
3	23249.5
4	44613.0
5	33840.5
6	23068.0
7	22138.0
8	21208.0
9	20741.0
10	20274.0
11	19479.5
12	18685.0
13	17956.5
14	17228.0
15	16295.0
16	15362.0
17	14468.5
18	13575.0
19	12742.5
20	11910.0
21	11054.5
22	10199.0
23	9756.5
24	9314.0
25	9117.5
26	9036.5
27	9152.0
28	9328.0
29	9504.0
30	9968.5
31	10433.0
32	11603.0
33	12773.0
34	13891.0
35	15009.0
36	16048.0
37	17087.0
38	18228.0
39	19369.0
40	20230.0
41	21091.0
42	21895.0
43	22699.0
44	25533.5
45	28368.0
46	63425.5
47	98483.0
48	68788.0
49	39093.0
50	36676.5
51	34260.0
52	30429.0
53	26598.0
54	25041.5
55	23485.0
56	21871.5
57	20258.0
58	18461.0
59	16664.0
60	14624.0
61	12584.0
62	11207.5
63	9831.0
64	8436.0
65	7041.0
66	5739.0
67	4437.0
68	3789.5
69	3142.0
70	2636.5
71	2131.0
72	1742.5
73	1354.0
74	1113.5
75	725.0
76	577.0
77	419.5
78	262.0
79	200.5
80	139.0
81	109.0
82	79.0
83	58.5
84	38.0
85	22.5
86	7.0
87	6.5
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	683128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.044301560507854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45694634764416	39.61586396084833
2	6.2670452837556425	6.021916270122293
3	2.8959472484074205	4.174012887174272
4	1.8948389942143258	3.641448641865699
5	1.428195488185212	3.4308327360863524
6	1.0818606716387549	3.1186344212799546
7	0.8521720580541816	2.8659407937015593
8	0.6384159108150756	2.4537797232180627
9	0.5268122161676575	2.277929248138655
>10	1.865723470024806	12.984799052981543
>50	0.05547331418163073	1.9360497723387267
>100	0.031300582101919355	2.908764567674958
>500	0.0015495337674216673	0.486283019964483
>1k	0.0024792540278746676	3.359760056796864
>5k	0.0	0.0
>10k+	0.0012396270139373338	10.723984847808293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21243	3.109666124064597	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	18546	2.714864564181237	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	16947	2.480794228900001	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	15289	2.2380871520417847	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	3925	0.5745628930449345	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	3546	0.5190828073216147	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	3434	0.5026876368703962	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	3304	0.4836575283109461	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	3053	0.44691478024616177	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	2811	0.41148950123549316	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT	1443	0.21123420500989565	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACATGAGTCGT	1049	0.15355833752971623	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTAT	727	0.10642222248246301	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06236020189481327	0.0
2	0.0	0.0	0.0	0.6158435900738953	0.0
3	0.0	0.0	0.0	0.7140682273307492	0.0
4	0.0	0.0	0.0	1.408959960651591	0.0
5	0.0	0.0	0.0	4.319395486643791	0.0
6	0.0	0.0	0.0	4.626951318054596	0.0
7	0.0	0.0	0.0	5.200782283847244	0.0
8	0.0	0.0	0.0	5.859663196355588	0.0
9	1.4638545045730813E-4	0.0	0.0	5.9653534915857644	0.0
10	1.4638545045730813E-4	0.0	0.0	9.214085793584804	0.0
11	1.4638545045730813E-4	0.0	0.0	9.37686641449333	0.0
12	1.4638545045730813E-4	0.0	0.0	12.098318323945147	0.0
13	1.4638545045730813E-4	0.0	0.0	12.298134463819371	0.0
14	1.4638545045730813E-4	0.0	0.0	12.462671710133387	0.0
15	1.4638545045730813E-4	0.0	0.0	12.995807520698902	0.0
16	1.4638545045730813E-4	0.0	0.0	13.251396517197362	0.0
17	1.4638545045730813E-4	0.0	0.0	13.378166317293392	0.0
18	2.9277090091461627E-4	0.0	0.0	13.486198779730884	0.0
19	2.9277090091461627E-4	0.0	0.0	14.185921232916819	0.0
20	2.9277090091461627E-4	0.0	0.0	14.340065112248363	0.0
21	2.9277090091461627E-4	0.0	0.0	14.447219261983113	0.0
22	2.9277090091461627E-4	0.0	0.0	14.65889262334438	0.0
23	2.9277090091461627E-4	0.0	0.0	14.78185640172852	0.0
24	2.9277090091461627E-4	0.0	0.0	14.901599700202597	0.0
25	2.9277090091461627E-4	0.0	0.0	14.992505064936585	0.0
26	2.9277090091461627E-4	0.0	0.0	15.109467039851975	0.0
27	2.9277090091461627E-4	0.0	0.0	15.260975981075289	0.0
28	2.9277090091461627E-4	0.0	0.0	15.363445796395405	0.0
29	2.9277090091461627E-4	0.0	0.0	15.474991509643873	0.0
30	2.9277090091461627E-4	0.0	0.0	15.604396247848134	0.0
31	2.9277090091461627E-4	0.0	0.0	15.723846775421297	0.0
32	2.9277090091461627E-4	0.0	0.0	15.857057535337448	0.0
33	2.9277090091461627E-4	0.0	0.0	15.986023117190337	0.0
34	2.9277090091461627E-4	0.0	0.0	16.178959140893067	0.0
35	2.9277090091461627E-4	0.0	0.0	16.33764096918879	0.0
36	2.9277090091461627E-4	0.0	0.0	16.47363305266363	0.0
37	2.9277090091461627E-4	0.0	0.0	16.628362473797004	0.0
38	2.9277090091461627E-4	0.0	0.0	16.797876825426567	0.0
39	2.9277090091461627E-4	0.0	0.0	17.012917052148353	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATG	20	7.0312753E-4	45.0	1
TAAGGCG	20	7.0312753E-4	45.0	29
CCTTGCG	45	3.8380676E-10	45.0	1
GTACGGG	45	3.8380676E-10	45.0	3
CGTTTTT	19980	0.0	44.481983	1
ACGGCTG	1845	0.0	42.682926	8
TACGGCT	1850	0.0	42.567566	7
GATACCT	2255	0.0	42.206207	5
TGATACC	2285	0.0	41.750546	4
CGGCTGT	1915	0.0	41.240208	9
ATACCTG	2320	0.0	41.12069	6
CCGATGA	450	0.0	41.0	18
GGCGACT	425	0.0	40.764706	10
GAATGAT	7070	0.0	40.09901	1
GCGACTG	405	0.0	40.0	11
CGATGAA	450	0.0	40.0	19
ATGATAC	6755	0.0	39.937084	3
GAATCTG	2035	0.0	39.914005	1
CGCTTTT	565	0.0	39.82301	1
CGGGATA	85	0.0	39.705883	6
>>END_MODULE