FastQCFastQC Report
Sat 14 Jan 2017
SRR2933415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447557
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266305.950080101528967No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG85261.9050087474891466No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC59531.3301099077882816No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC48581.085448333955228No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC16300.36419942040902054No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT15810.35325109427402546No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT15060.33649345223066557No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC11790.26343013292161666No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA8600.19215429543052615No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT7180.16042649316176488No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGACATGAGTCGTATGCCG6870.15350000111717613No Hit
GAATCTTTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC5180.1157394477128053No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4650.103897380668831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT149500.044.5936471
GATACCT10600.042.02835
TACGGCT8000.041.906257
CCGATGA1450.041.8965518
CGATGAA1400.041.78571719
TTGTTCG603.6379788E-1241.2499961
TGATACC10850.040.8525354
GAATGAT31050.040.579711
CGGCTGT7900.040.4430359
ACGGCTG8300.040.3915678
GTTTTTT167450.040.203052
ATGATAC30150.040.03
AATGATA29700.039.7727282
ATACGGC19250.039.623386
TGCGGGA3750.039.6000024
ATACCTG11250.039.66
CGACCAC6850.039.41605812
ATGGGCG403.4519144E-739.3755
TCGTGCG403.4519144E-739.3751
CACCGGT1150.039.13043616