##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933415.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	447557
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.61923285749078	31.0	28.0	31.0	16.0	33.0
2	28.896710363149275	31.0	30.0	33.0	16.0	34.0
3	29.028492460178256	31.0	30.0	33.0	16.0	34.0
4	32.17115585277406	35.0	30.0	37.0	19.0	37.0
5	33.719711679182765	35.0	33.0	37.0	28.0	37.0
6	32.81803435093184	35.0	33.0	37.0	17.0	37.0
7	32.2801453222718	35.0	32.0	35.0	25.0	37.0
8	32.97491045833268	35.0	32.0	36.0	27.0	37.0
9	33.0678528098097	35.0	31.0	37.0	25.0	39.0
10	32.10728019000932	35.0	28.0	39.0	15.0	39.0
11	32.77659381933474	37.0	30.0	39.0	17.0	39.0
12	33.51972374468504	37.0	31.0	39.0	25.0	39.0
13	33.65528860011127	35.0	32.0	39.0	25.0	39.0
14	33.828220762941925	37.0	32.0	40.0	21.0	41.0
15	34.37197273196487	37.0	32.0	40.0	25.0	41.0
16	34.560525251532205	37.0	32.0	40.0	25.0	41.0
17	32.607408665264984	36.0	29.0	40.0	18.0	41.0
18	33.24869457968482	36.0	30.0	39.0	23.0	39.0
19	32.998395735068385	36.0	31.0	37.0	22.0	39.0
20	32.4680118956915	34.0	31.0	36.0	24.0	39.0
21	32.7939681426053	35.0	31.0	37.0	24.0	39.0
22	32.89912793230806	34.0	31.0	37.0	25.0	39.0
23	33.907790516068346	35.0	32.0	38.0	27.0	40.0
24	32.14057427322106	35.0	31.0	37.0	21.0	39.0
25	31.34126155998007	34.0	29.0	37.0	18.0	39.0
26	30.462414843249018	33.0	25.0	37.0	18.0	39.0
27	32.315792178426435	34.0	30.0	37.0	22.0	39.0
28	32.33784076665095	35.0	31.0	37.0	21.0	39.0
29	32.39824648033658	35.0	31.0	38.0	21.0	40.0
30	31.44495114588756	34.0	29.0	37.0	18.0	40.0
31	31.784431927106493	35.0	30.0	37.0	20.0	40.0
32	31.465701575441788	35.0	30.0	37.0	17.0	40.0
33	31.136972497357878	35.0	30.0	38.0	15.0	40.0
34	30.017043639134233	35.0	25.0	38.0	8.0	40.0
35	29.32503569377755	35.0	23.0	38.0	7.0	40.0
36	28.68637514327784	34.0	21.0	38.0	7.0	40.0
37	28.655858359940744	34.0	21.0	38.0	7.0	40.0
38	28.689429502834276	34.0	21.0	38.0	7.0	40.0
39	28.239332643663264	34.0	19.0	38.0	7.0	40.0
40	28.000507197965845	33.0	18.0	38.0	7.0	40.0
41	27.689371409675193	33.0	18.0	37.0	7.0	40.0
42	27.923069016907345	33.0	18.0	38.0	7.0	40.0
43	27.93385423532645	33.0	18.0	38.0	7.0	40.0
44	27.871283434288816	33.0	18.0	38.0	7.0	40.0
45	28.151565052049236	34.0	18.0	38.0	7.0	40.0
46	27.85592673112028	34.0	18.0	38.0	7.0	40.0
47	27.85002580676875	34.0	18.0	38.0	7.0	40.0
48	27.631351984216536	33.0	18.0	37.0	7.0	40.0
49	27.44011153886544	33.0	18.0	37.0	7.0	39.0
50	27.596578759800426	33.0	18.0	37.0	7.0	40.0
51	26.066494323628053	31.0	15.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	18.0
11	28.0
12	36.0
13	52.0
14	112.0
15	253.0
16	533.0
17	980.0
18	1703.0
19	2899.0
20	4184.0
21	6296.0
22	8664.0
23	11802.0
24	16115.0
25	22010.0
26	26696.0
27	27060.0
28	25014.0
29	24647.0
30	26825.0
31	29596.0
32	33947.0
33	38055.0
34	42096.0
35	44485.0
36	32431.0
37	17382.0
38	3568.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.49715008367649	2.390310061064848	33.6949260094245	42.41761384583416
2	43.59980963318639	11.215107796325384	32.18495074370416	13.000131826784076
3	11.715602705353731	11.251080867911798	63.88080177496944	13.152514651765026
4	10.67193675889328	2.684127384891757	71.22355364791524	15.420382208299724
5	17.562232296668356	3.1620553359683794	63.67568823635872	15.600024131004542
6	12.997450604057137	10.100389447601088	65.85753323040417	11.044626717937604
7	49.9581058948916	1.0966200953174678	46.28661824080508	2.6586557689858497
8	50.90904622204546	7.516137609287756	38.32025864861906	3.254557520047726
9	46.87201853618645	2.750264212156217	38.49565530200623	11.882061949651106
10	24.828345886669183	18.24259256362877	44.84791881257583	12.081142737126221
11	20.30311222928029	15.834631119611581	51.270564419727535	12.591692231380582
12	16.65843680246315	14.152387293685498	53.266958175159814	15.92221772869154
13	15.539473184421201	15.041436062892547	57.82615398708991	11.592936765596336
14	14.4636325652375	17.274671159204303	52.70300766159394	15.558688613964256
15	11.945294118961383	15.229791959459913	58.4111074120168	14.41380650956191
16	13.481187871042124	15.346425148081696	51.5391335628758	19.633253418000386
17	14.063236638014823	16.032371295723227	54.73470418293089	15.169687883331061
18	14.027487001655656	14.694217719754132	54.392401414791856	16.885893863798355
19	14.185455707317725	17.971565632980827	50.964234723174926	16.878743936526522
20	15.706379299173065	16.82333200016981	55.10069108515787	12.369597615499254
21	16.00086692868171	19.61247394186662	51.17225291973983	13.214406209711834
22	14.427882928878333	14.885254839048434	53.573287871712424	17.113574360360804
23	14.075078705058797	19.863168266835284	52.01125219804405	14.050500830061868
24	14.573115826587452	16.225642767289976	52.062642300310344	17.138599105812222
25	13.355617273330548	20.59536550651649	49.9665964335269	16.082420786626063
26	13.991067059614753	16.56347683088411	52.7047951434119	16.740660966089234
27	14.467654399327905	18.287056173850484	50.41167940619854	16.833610020623073
28	12.572700237064776	17.613398963707418	53.64456370920352	16.16933709002429
29	17.046990662641853	16.273011035465874	50.478263103917485	16.201735197974784
30	13.868177684630115	19.854230857745495	51.862220901471765	14.415370556152624
31	16.097390946851462	17.39108091259884	48.48008186666726	18.03144627388243
32	16.742001577452704	21.463634799589773	47.40446468271081	14.389898940246718
33	15.454567798068178	18.199246129543276	46.62780383280789	19.718382239580656
34	20.683845856505428	20.620390251967905	42.82538313555592	15.870380755970748
35	15.375918598078009	25.78397835359518	41.04907307895977	17.79102996936703
36	20.053088209993362	20.992856775784986	39.146522118970324	19.80753289525133
37	16.578446991109512	26.934222903451406	40.09098282453408	16.396347280905
38	17.644009589839953	22.663928840348827	41.03991223464274	18.65214933516848
39	21.228580940528246	23.458687943658575	38.5763154190416	16.736415696771584
40	18.405476844290224	24.357344427637152	40.91925721193055	16.31792151614208
41	22.01730729270238	23.133142817562902	37.41043040327824	17.439119486456473
42	17.451855294409427	24.612730892377954	40.24872809496891	17.686685718243712
43	21.261649354160475	21.17942519053439	38.20630668272421	19.352618772580925
44	19.87456346342477	22.646500892623735	37.992702605478186	19.486233038473312
45	17.202948451258724	21.873415006356733	38.72534671561388	22.19828982677067
46	20.43114061449156	23.854615166336355	36.949036659017736	18.765207560154348
47	16.599896772925014	21.441514712092538	43.36721356162455	18.5913749533579
48	19.296983400996968	23.148559848242794	37.01539692150944	20.5390598292508
49	16.705805070639045	21.980217044979746	40.55237656879459	20.761601315586617
50	18.829333470373605	20.582629698563533	39.26851775304598	21.319519078016878
51	18.49194627723396	20.60854818492393	37.867355442993855	23.03215009484825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	383.5
2	697.0
3	20770.5
4	40844.0
5	28386.0
6	15928.0
7	15011.0
8	14094.0
9	13770.0
10	13446.0
11	12828.5
12	12211.0
13	11525.5
14	10840.0
15	10148.0
16	9456.0
17	8830.5
18	8205.0
19	7528.0
20	6851.0
21	6425.5
22	6000.0
23	5749.0
24	5498.0
25	5353.5
26	5194.0
27	5179.0
28	5377.5
29	5576.0
30	6035.5
31	6495.0
32	7135.0
33	7775.0
34	8724.0
35	9673.0
36	10382.0
37	11091.0
38	11988.0
39	12885.0
40	13559.0
41	14233.0
42	15010.5
43	15788.0
44	17567.5
45	19347.0
46	32531.5
47	45716.0
48	37647.5
49	29579.0
50	27524.0
51	25469.0
52	22324.0
53	19179.0
54	17560.0
55	15941.0
56	14566.5
57	13192.0
58	12148.0
59	11104.0
60	9736.0
61	8368.0
62	7524.0
63	6680.0
64	5709.5
65	4739.0
66	4012.5
67	3286.0
68	2846.0
69	2406.0
70	2040.5
71	1675.0
72	1422.5
73	1170.0
74	942.0
75	596.0
76	478.0
77	354.5
78	231.0
79	181.5
80	132.0
81	95.5
82	59.0
83	45.5
84	32.0
85	19.5
86	7.0
87	6.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	447557.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.928561285983285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08377623910663	41.8040862877828
2	7.021819458041207	7.1522232521592315
3	3.145489883972835	4.8058582299105295
4	1.9709270122356213	4.015059085313671
5	1.3996820988169072	3.564189777524528
6	1.0145166099607166	3.1000722807619416
7	0.7891519494449069	2.8133261394880686
8	0.5912292962966392	2.408836596040973
9	0.4309030536681589	1.975074531935056
>10	1.4630950130742286	10.74905225081524
>50	0.05051630274100384	1.7881560582427771
>100	0.033528519518255195	3.3825610589822572
>500	0.0017881877076402772	0.6336183231572742
>1k	0.0022352346345503463	2.4484123058689637
>5k	8.940938538201386E-4	3.2965000722221243
>10k+	4.470469269100693E-4	6.062973749794542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26630	5.950080101528967	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	8526	1.9050087474891466	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	5953	1.3301099077882816	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	4858	1.085448333955228	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1630	0.36419942040902054	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	1581	0.35325109427402546	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	1506	0.33649345223066557	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	1179	0.26343013292161666	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	860	0.19215429543052615	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	718	0.16042649316176488	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGACATGAGTCGTATGCCG	687	0.15350000111717613	No Hit
GAATCTTTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	518	0.1157394477128053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	465	0.103897380668831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09272561930659111	0.0
2	0.0	0.0	0.0	0.6224905431040069	0.0
3	0.0	0.0	0.0	0.7771077203574069	0.0
4	0.0	0.0	0.0	1.3540174771034752	0.0
5	0.0	0.0	0.0	2.8293602826008755	0.0
6	0.0	0.0	0.0	3.175908320057557	0.0
7	0.0	0.0	0.0	3.553066983646776	0.0
8	0.0	0.0	0.0	4.080597555171743	0.0
9	0.0	0.0	0.0	4.260686348331051	0.0
10	0.0	0.0	0.0	6.804719845740319	0.0
11	0.0	0.0	0.0	7.277061916135822	0.0
12	0.0	0.0	0.0	9.184751886351906	0.0
13	2.2343522724479787E-4	0.0	0.0	9.398132528370688	0.0
14	2.2343522724479787E-4	0.0	0.0	9.539120156762156	0.0
15	2.2343522724479787E-4	0.0	0.0	9.902649271489441	0.0
16	2.2343522724479787E-4	0.0	0.0	10.140607788505152	0.0
17	2.2343522724479787E-4	0.0	0.0	10.377672564611881	0.0
18	2.2343522724479787E-4	0.0	0.0	10.621216962308711	0.0
19	2.2343522724479787E-4	0.0	0.0	11.240355977004047	0.0
20	2.2343522724479787E-4	0.0	0.0	11.480548846292205	0.0
21	2.2343522724479787E-4	0.0	0.0	11.7383930985327	0.0
22	2.2343522724479787E-4	0.0	0.0	12.089186405307034	0.0
23	2.2343522724479787E-4	0.0	0.0	12.36937418027201	0.0
24	2.2343522724479787E-4	0.0	0.0	12.58409543365426	0.0
25	2.2343522724479787E-4	0.0	0.0	12.780718433629684	0.0
26	2.2343522724479787E-4	0.0	0.0	12.95611508701685	0.0
27	2.2343522724479787E-4	0.0	0.0	13.145811594947682	0.0
28	2.2343522724479787E-4	0.0	0.0	13.330815963106375	0.0
29	2.2343522724479787E-4	0.0	0.0	13.527215527854553	0.0
30	2.2343522724479787E-4	0.0	0.0	13.764727174415773	0.0
31	2.2343522724479787E-4	0.0	0.0	13.968276666435784	0.0
32	2.2343522724479787E-4	0.0	0.0	14.17070898231957	0.0
33	2.2343522724479787E-4	0.0	0.0	14.37738656752101	0.0
34	4.4687045448959573E-4	0.0	0.0	14.585404764085915	0.0
35	4.4687045448959573E-4	0.0	0.0	14.813979001557344	0.0
36	4.4687045448959573E-4	0.0	0.0	15.010378566305521	0.0
37	4.4687045448959573E-4	0.0	0.0	15.22733417196022	0.0
38	4.4687045448959573E-4	0.0	0.0	15.463058336703481	0.0
39	4.4687045448959573E-4	0.0	0.0	15.757545966212126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14950	0.0	44.593647	1
GATACCT	1060	0.0	42.0283	5
TACGGCT	800	0.0	41.90625	7
CCGATGA	145	0.0	41.89655	18
CGATGAA	140	0.0	41.785717	19
TTGTTCG	60	3.6379788E-12	41.249996	1
TGATACC	1085	0.0	40.852535	4
GAATGAT	3105	0.0	40.57971	1
CGGCTGT	790	0.0	40.443035	9
ACGGCTG	830	0.0	40.391567	8
GTTTTTT	16745	0.0	40.20305	2
ATGATAC	3015	0.0	40.0	3
AATGATA	2970	0.0	39.772728	2
ATACGGC	1925	0.0	39.62338	6
TGCGGGA	375	0.0	39.600002	4
ATACCTG	1125	0.0	39.6	6
CGACCAC	685	0.0	39.416058	12
ATGGGCG	40	3.4519144E-7	39.375	5
TCGTGCG	40	3.4519144E-7	39.375	1
CACCGGT	115	0.0	39.130436	16
>>END_MODULE