SRR2933414.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933414.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655249
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	24267	3.703477609275253	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	20441	3.1195774430788905	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	19872	3.0327402254715383	RNA PCR Primer, Index 29 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18872	2.8801264862670526	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT	4971	0.7586428975854981	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	4772	0.7282727634838054	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA	4510	0.6882879638122301	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	4490	0.6852356890281405	TruSeq Adapter, Index 12 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	4186	0.6388411123099769	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	4073	0.6215957597798699	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGT	1610	0.24570812011922186	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	1085	0.1655859070368669	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG	1047	0.15978658494709644	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	1043	0.1591761299902785	RNA PCR Primer, Index 29 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	670	0.10225120526700536	RNA PCR Primer, Index 29 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAT	20	7.0310646E-4	45.0	8
TAGGGCG	50	2.1827873E-11	45.0	5
CGCGTCC	20	7.0310646E-4	45.0	21
CTGCGAT	30	2.164068E-6	44.999996	31
CACTCGA	30	2.164068E-6	44.999996	44
TGCGATC	30	2.164068E-6	44.999996	32
ACTCGAG	30	2.164068E-6	44.999996	45
TAATGCG	30	2.164068E-6	44.999996	24
CGTTTTT	14825	0.0	44.392914	1
TACGGCT	2650	0.0	42.45283	7
TGATACC	2885	0.0	42.34835	4
GATACCT	2880	0.0	42.26562	5
ACGGCTG	2660	0.0	42.20865	8
CACCGGT	230	0.0	42.06522	16
CCGATGA	595	0.0	41.97479	18
CGGCTGT	2740	0.0	41.468975	9
CGATGAA	625	0.0	41.04	19
CGACCAC	2195	0.0	41.00228	12
CACCCGT	165	0.0	40.90909	16
ATACCTG	2985	0.0	40.85427	6