FastQCFastQC Report
Sat 14 Jan 2017
SRR2933413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431216
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277426.433434751957255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG130553.027485065489221No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC97782.267541093094876No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC70591.6369986271381396RNA PCR Primer, Index 29 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC29790.690837074691106Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT27430.6361081221476013No Hit
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT23310.5405643575377537TruSeq Adapter, Index 12 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC18800.43597640161775075No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA17550.406988608956996No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT12490.289646024266261No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCACTAACCTCGTATGCCG11130.2581073058513599No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT8200.19015991985455086RNA PCR Primer, Index 29 (96% over 26bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGT6410.14864940076435013No Hit
GAATCTTTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC5790.13427145560461579No Hit
GAATGACTCTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT5410.12545916663574636No Hit
GAATCTGTCTCTTATCCACATCTGACGCCACTAACCTCGTATGCCGTCTTC5050.11711068234944899No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT4580.10621127230900523RNA PCR Primer, Index 29 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT4540.10528366294386107No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC4490.1041241512374309Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC4440.10296463953100071RNA PCR Primer, Index 29 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4400.10203703016585655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG351.2095734E-745.0000041
CCGGGTA253.886737E-545.0000045
CTTTGCG502.1827873E-1145.0000041
CGGTAGT302.1623346E-645.00000412
TACGATT207.028379E-445.045
GTACGGG207.028379E-445.03
CGTTTTT116150.044.341371
CCGATGA1600.043.5937518
TACGGCT15150.043.069317
CGATGAA1600.042.187519
ACGGCTG15450.042.087388
GAATGAT48850.041.9600831
TGATACC16850.041.394664
GATACCT16850.041.394665
TCGTCCC603.6379788E-1241.25000438
AATGATA46700.041.241972
CGACCAC8650.041.09826712
ATGATAC47500.041.0210533
TCGTTCA556.002665E-1140.90909216
ACCGGTG1100.040.90909217
Produced by FastQC (version 0.11.5)