##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933413.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431216
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.87394484434715	31.0	25.0	31.0	16.0	33.0
2	27.85857435716671	31.0	27.0	33.0	16.0	34.0
3	28.0135778820823	31.0	27.0	33.0	16.0	34.0
4	31.411046899929502	35.0	28.0	35.0	19.0	37.0
5	33.49483553485956	35.0	33.0	37.0	28.0	37.0
6	31.9189269414864	35.0	32.0	37.0	17.0	37.0
7	32.055760917962225	35.0	32.0	35.0	25.0	37.0
8	32.32235584950465	35.0	32.0	35.0	23.0	37.0
9	32.82697070609625	35.0	31.0	37.0	25.0	39.0
10	31.649090015212792	35.0	28.0	39.0	15.0	39.0
11	32.22280945048421	35.0	27.0	39.0	17.0	39.0
12	33.05957803049979	35.0	31.0	39.0	23.0	39.0
13	33.14778904307818	35.0	31.0	39.0	23.0	39.0
14	33.348688360357684	36.0	31.0	39.0	19.0	41.0
15	33.80014656227969	36.0	31.0	39.0	23.0	41.0
16	33.94649317279507	36.0	32.0	40.0	25.0	41.0
17	31.92637564468851	34.0	27.0	39.0	18.0	41.0
18	32.7245208897629	35.0	30.0	39.0	21.0	39.0
19	32.47425188304701	35.0	30.0	37.0	18.0	39.0
20	32.03843317502133	34.0	30.0	36.0	23.0	39.0
21	32.60670986234277	35.0	31.0	37.0	23.0	39.0
22	32.95322065971578	35.0	31.0	37.0	25.0	39.0
23	33.862277837557045	35.0	32.0	38.0	27.0	40.0
24	32.35255881043375	35.0	31.0	37.0	22.0	39.0
25	31.514454472932357	35.0	30.0	37.0	18.0	39.0
26	30.870765463248116	33.0	26.0	37.0	19.0	39.0
27	32.54830525768988	35.0	31.0	37.0	22.0	39.0
28	32.380711754665874	35.0	31.0	37.0	21.0	39.0
29	32.55996994545657	35.0	31.0	38.0	21.0	40.0
30	31.536081685280696	35.0	29.0	38.0	18.0	40.0
31	31.936836759303922	35.0	30.0	37.0	21.0	40.0
32	31.854451133538642	35.0	30.0	37.0	18.0	40.0
33	31.781589736929984	35.0	30.0	38.0	16.0	40.0
34	31.129088438276874	35.0	30.0	38.0	13.0	40.0
35	30.602095005751178	35.0	28.0	38.0	10.0	40.0
36	30.08185225038032	35.0	26.0	38.0	9.0	40.0
37	30.221153667767428	35.0	26.0	38.0	8.0	40.0
38	30.021724611331678	35.0	25.0	38.0	8.0	40.0
39	29.585952283774258	34.0	24.0	38.0	8.0	40.0
40	29.30543857370784	34.0	23.0	38.0	8.0	40.0
41	28.79085191644095	33.0	22.0	37.0	8.0	40.0
42	29.37208266854662	34.0	23.0	38.0	8.0	40.0
43	29.53655012801009	35.0	23.0	38.0	8.0	40.0
44	29.370607769656043	34.0	23.0	38.0	7.0	40.0
45	29.68403074097436	35.0	23.0	38.0	7.0	40.0
46	29.337999981447812	34.0	23.0	38.0	7.0	40.0
47	29.386611350228193	35.0	23.0	38.0	7.0	40.0
48	29.186312660012614	34.0	23.0	38.0	7.0	40.0
49	29.12546380468257	34.0	23.0	37.0	7.0	40.0
50	29.293340228562947	34.0	24.0	38.0	7.0	40.0
51	27.606923212496753	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	20.0
11	36.0
12	36.0
13	41.0
14	82.0
15	174.0
16	374.0
17	777.0
18	1373.0
19	2310.0
20	3552.0
21	4857.0
22	6833.0
23	9132.0
24	12457.0
25	16609.0
26	21309.0
27	23841.0
28	24125.0
29	25809.0
30	29304.0
31	33694.0
32	38042.0
33	41338.0
34	43565.0
35	44077.0
36	29780.0
37	14637.0
38	2960.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.427516604207636	2.4945734852139068	31.81537790805536	37.262532002523095
2	38.29426551890468	17.084013580201105	30.780397758895774	13.84132314199844
3	12.434835442098624	17.188137731438534	56.97863715632073	13.39838967014211
4	11.46641126488813	2.8623705984935626	69.69917628288376	15.972041853734556
5	22.225752291195132	3.24408185225038	57.547725501836666	16.98244035471782
6	13.403491521650402	14.839430818893549	59.78140885310378	11.97566880635227
7	48.87434603539758	1.548180030425587	46.20399057548885	3.373483358687989
8	48.60232458906905	11.823772772809914	34.295341545768245	5.278561092352788
9	44.84991280471968	3.0100923898927685	34.38439204482208	17.75560276056547
10	27.677544432488588	18.12502319023413	39.96257096211643	14.234861415160848
11	24.0981317947386	15.381386590479018	46.660374383139775	13.86010723164261
12	19.0257318837891	13.42876887685058	49.02786538532893	18.51763385403139
13	17.52509183332715	15.390198879447887	55.28783718600423	11.796872101220735
14	15.0727245742273	19.14748061296427	46.915003153871844	18.86479165893659
15	11.377360765834291	15.334542317539238	57.32996920336908	15.958127713257392
16	13.053087083967199	15.505686245408334	46.616776742978	24.82444992764647
17	13.644901858929167	17.00423917479871	52.046770064190575	17.304088902081556
18	13.532197321064155	15.548124373863677	51.08553485955994	19.834143445512225
19	13.643510444881452	18.952218841601425	46.899001892323106	20.50526882119402
20	15.544645838744389	17.95248784831732	53.66104226188268	12.84182405105562
21	16.64084820600349	22.228998923973137	46.775861749100216	14.354291120923158
22	13.732792846276576	14.912016251716079	50.58601721642981	20.76917368557753
23	14.104068494675523	22.16383436607176	48.082167637564474	15.649929501688248
24	16.08010834477385	16.531622203257765	46.65434492226633	20.73392452970205
25	13.203823605803125	23.638965158992246	44.63911357649067	18.518097658713963
26	13.535212051500872	16.245918518793363	49.23750510185151	20.981364327854255
27	14.826676190122814	19.299144744165336	46.00571407368928	19.86846499202256
28	10.858827130718712	18.158649029720603	51.082288226781934	19.900235612778747
29	16.220873065934473	16.03210456012764	46.54882935698119	21.198193016956697
30	13.447552966494749	23.80500723535305	46.956281770620755	15.791158027531447
31	16.050424845089236	17.342120886052463	43.786872472264484	22.82058179659382
32	17.368557752959074	21.36307558161107	44.30958034952321	16.958786315906647
33	14.137926236503285	20.60591443731216	40.99476828318059	24.26139104300397
34	16.63481874513005	21.171988052391377	40.640885310378096	21.55230789210048
35	12.917192311973583	24.76299580720567	38.914140477162256	23.40567140365849
36	15.139048643835107	20.850803309710216	35.9437033876294	28.066444658825272
37	14.360320581796593	24.420244146784906	43.04640829653816	18.173026974880337
38	16.702534228785574	24.456652814366812	36.023709695373086	22.81710326147453
39	16.976642796185672	24.34742681162109	40.14275908129569	18.533171310897558
40	16.078021223702272	24.076101072316426	37.80587918815628	22.039998515825015
41	15.792549441579162	24.948749582575786	35.070822975028754	24.187878000816294
42	15.841017030907945	20.092714556046158	40.97273756075842	23.093530852287486
43	17.621795109643426	19.63262031093466	35.900569552150195	26.845015027271717
44	19.074663277800454	21.695391636673964	37.73306185299246	21.496883232533115
45	16.161737968906532	19.63957738117324	37.33001558383734	26.86866906608289
46	20.42781343920448	22.450233757560017	36.34930058253868	20.77265222069682
47	14.115199807057252	20.958637898408224	44.60316871359134	20.322993580943194
48	17.7667340729472	23.495185707394903	35.56686208303959	23.171218136618307
49	16.04787391933509	18.68251641868576	41.576611257467256	23.692998404511894
50	17.708526585284403	18.545230232644432	38.4350302400653	25.311212942005863
51	17.69414864012467	19.035935586805685	36.50676227227191	26.763153500797742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	434.0
2	782.0
3	20323.0
4	39864.0
5	26528.5
6	13193.0
7	12426.0
8	11659.0
9	11310.0
10	10961.0
11	10285.0
12	9609.0
13	8774.5
14	7940.0
15	7142.5
16	6345.0
17	5818.0
18	5291.0
19	4806.0
20	4321.0
21	3999.0
22	3677.0
23	3489.0
24	3301.0
25	3362.5
26	3617.0
27	3810.0
28	4013.0
29	4216.0
30	4533.5
31	4851.0
32	5349.5
33	5848.0
34	6543.5
35	7239.0
36	7937.0
37	8635.0
38	9328.0
39	10021.0
40	10867.5
41	11714.0
42	12946.0
43	14178.0
44	16272.0
45	18366.0
46	39845.0
47	61324.0
48	49500.0
49	37676.0
50	33938.5
51	30201.0
52	25473.5
53	20746.0
54	18467.5
55	16189.0
56	14820.0
57	13451.0
58	12408.5
59	11366.0
60	10205.0
61	9044.0
62	7955.0
63	6866.0
64	5872.0
65	4878.0
66	4082.5
67	3287.0
68	2797.0
69	2307.0
70	1986.5
71	1666.0
72	1399.5
73	1133.0
74	941.0
75	619.0
76	489.0
77	399.0
78	309.0
79	216.0
80	123.0
81	87.5
82	52.0
83	32.5
84	13.0
85	10.5
86	8.0
87	7.5
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.89728436286509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08297041229359	34.39053552712507
2	6.590855193412613	5.52277868465749
3	2.644885796292166	3.3244059694366723
4	1.5789081846325381	2.646078607776182
5	1.1468575812901154	2.402510910350982
6	0.9501542962417148	2.388533084294225
7	0.7619611739184916	2.234687278398789
8	0.6381107025166344	2.1388084446661635
9	0.5361224014398623	2.0215865435785223
>10	2.944455273968999	19.282007982784087
>50	0.06997807246681526	2.036009285036895
>100	0.045711482821064804	3.933226623689321
>500	0.0028216964704360988	0.7296626005834232
>1k	0.003950375058610538	3.3220218853522674
>5k	0.0011286785881744396	3.980988077129973
>10k+	0.0011286785881744396	9.64615849513996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27742	6.433434751957255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	13055	3.027485065489221	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	9778	2.267541093094876	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	7059	1.6369986271381396	RNA PCR Primer, Index 29 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	2979	0.690837074691106	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT	2743	0.6361081221476013	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	2331	0.5405643575377537	TruSeq Adapter, Index 12 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	1880	0.43597640161775075	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA	1755	0.406988608956996	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	1249	0.289646024266261	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCACTAACCTCGTATGCCG	1113	0.2581073058513599	No Hit
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	820	0.19015991985455086	RNA PCR Primer, Index 29 (96% over 26bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGT	641	0.14864940076435013	No Hit
GAATCTTTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	579	0.13427145560461579	No Hit
GAATGACTCTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	541	0.12545916663574636	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCACTAACCTCGTATGCCGTCTTC	505	0.11711068234944899	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	458	0.10621127230900523	RNA PCR Primer, Index 29 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	454	0.10528366294386107	No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	449	0.1041241512374309	Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	444	0.10296463953100071	RNA PCR Primer, Index 29 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	440	0.10203703016585655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23932321620719083	0.0
2	0.0	0.0	0.0	1.4088067233126786	0.0
3	0.0	0.0	0.0	1.6680735408704686	0.0
4	0.0	0.0	0.0	2.679863455901451	0.0
5	0.0	0.0	0.0	5.016047642016994	0.0
6	0.0	0.0	0.0	5.469880523913769	0.0
7	0.0	0.0	0.0	6.081175095543765	0.0
8	0.0	0.0	0.0	6.916719231197358	0.0
9	0.0	0.0	0.0	7.142128306927387	0.0
10	0.0	0.0	0.0	11.226856146339653	0.0
11	0.0	0.0	0.0	12.077242031835553	0.0
12	0.0	0.0	0.0	15.392749805202033	0.0
13	0.0	0.0	0.0	15.789534711142444	0.0
14	0.0	0.0	0.0	16.003812474490744	0.0
15	0.0	0.0	0.0	16.714825052873735	0.0
16	0.0	0.0	0.0	17.13990204445104	0.0
17	0.0	0.0	0.0	17.521149493525286	0.0
18	0.0	0.0	0.0	17.87688768505807	0.0
19	0.0	0.0	0.0	19.006715891803644	0.0
20	0.0	0.0	0.0	19.393528997068753	0.0
21	0.0	0.0	0.0	19.79008200066788	0.0
22	0.0	0.0	0.0	20.411812177655747	0.0
23	0.0	0.0	0.0	20.814858446810877	0.0
24	0.0	0.0	0.0	21.134419873103038	0.0
25	0.0	0.0	0.0	21.42221067863901	0.0
26	0.0	0.0	0.0	21.664780527624206	0.0
27	2.3190234128603763E-4	0.0	0.0	21.9156988608957	0.0
28	2.3190234128603763E-4	0.0	0.0	22.16731290119105	0.0
29	2.3190234128603763E-4	0.0	0.0	22.423564988312123	0.0
30	2.3190234128603763E-4	0.0	0.0	22.727125153055546	0.0
31	2.3190234128603763E-4	0.0	0.0	22.96436124819116	0.0
32	2.3190234128603763E-4	0.0	0.0	23.228266112574673	0.0
33	2.3190234128603763E-4	0.0	0.0	23.486373418426034	0.0
34	2.3190234128603763E-4	0.0	0.0	23.75051018515083	0.0
35	2.3190234128603763E-4	0.0	0.0	24.04479425624281	0.0
36	2.3190234128603763E-4	0.0	0.0	24.283885570108716	0.0
37	2.3190234128603763E-4	0.0	0.0	24.539210047864643	0.0
38	2.3190234128603763E-4	0.0	0.0	24.818884271455605	0.0
39	2.3190234128603763E-4	0.0	0.0	25.203610255649142	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	35	1.2095734E-7	45.000004	1
CCGGGTA	25	3.886737E-5	45.000004	5
CTTTGCG	50	2.1827873E-11	45.000004	1
CGGTAGT	30	2.1623346E-6	45.000004	12
TACGATT	20	7.028379E-4	45.0	45
GTACGGG	20	7.028379E-4	45.0	3
CGTTTTT	11615	0.0	44.34137	1
CCGATGA	160	0.0	43.59375	18
TACGGCT	1515	0.0	43.06931	7
CGATGAA	160	0.0	42.1875	19
ACGGCTG	1545	0.0	42.08738	8
GAATGAT	4885	0.0	41.960083	1
TGATACC	1685	0.0	41.39466	4
GATACCT	1685	0.0	41.39466	5
TCGTCCC	60	3.6379788E-12	41.250004	38
AATGATA	4670	0.0	41.24197	2
CGACCAC	865	0.0	41.098267	12
ATGATAC	4750	0.0	41.021053	3
TCGTTCA	55	6.002665E-11	40.909092	16
ACCGGTG	110	0.0	40.909092	17
>>END_MODULE