FastQCFastQC Report
Sat 14 Jan 2017
SRR2933412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985671
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282572.8667780628627604No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG232832.3621472073338876No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC227142.304420034676885No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC190301.9306644914986848No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT58980.5983741025149365No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCTTGTT48780.49489129740045107No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTC46400.47074530954040444No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC42780.4340190590978126No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCT37870.38420527742015337No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTA36400.3692915790360069No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTT20150.204429266966361No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGT16090.1632390523815756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCGT253.8903996E-545.044
CGTTTTT293450.044.4862821
CCGATGA7250.042.82758318
GATACCT27350.042.3674625
CGATGAA7050.042.12766319
TGATACC27650.041.82644
ACGGCTG23150.041.6954658
TACGGCT23100.041.688317
CGGCTGT23600.041.4724589
ATACCTG28150.041.163416
CGACCAC22450.040.38975512
ATGATAC88650.040.025383
GAATGAT92700.039.6601941
GCGACCA23300.039.59227411
ATACGGC61550.039.4069866
AATGATA88100.039.3813822
GCGATCG403.4575532E-739.3750049
GAATCTG29850.039.3467331
TACCTGT29650.039.30867
ACCACCG14200.039.13732514
Produced by FastQC (version 0.11.5)