##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933412.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	985671
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.502275099906562	31.0	28.0	33.0	25.0	34.0
2	28.78233203574012	31.0	28.0	33.0	16.0	34.0
3	28.77497258212933	31.0	28.0	34.0	16.0	34.0
4	33.1947424647778	35.0	32.0	37.0	28.0	37.0
5	34.293421435752904	35.0	33.0	37.0	30.0	37.0
6	33.7720902816457	35.0	33.0	37.0	28.0	37.0
7	33.72727005258347	35.0	33.0	37.0	29.0	37.0
8	33.649966368088336	35.0	33.0	37.0	28.0	37.0
9	34.86671820516176	37.0	34.0	39.0	28.0	39.0
10	34.79606176908928	37.0	33.0	39.0	28.0	39.0
11	35.126375839402805	37.0	34.0	39.0	28.0	39.0
12	35.28245124387346	37.0	34.0	39.0	30.0	39.0
13	35.47588698460237	37.0	34.0	39.0	30.0	39.0
14	36.22166422670445	38.0	34.0	40.0	30.0	41.0
15	36.59151177218362	38.0	35.0	40.0	30.0	41.0
16	36.50668935172081	38.0	34.0	41.0	30.0	41.0
17	35.74569912272959	38.0	33.0	40.0	27.0	41.0
18	35.48947163911691	38.0	34.0	39.0	27.0	40.0
19	34.781413879479054	37.0	33.0	38.0	27.0	40.0
20	33.99724248760489	35.0	33.0	38.0	27.0	40.0
21	34.31803715438519	35.0	33.0	38.0	27.0	40.0
22	34.754469797731694	35.0	33.0	39.0	29.0	40.0
23	35.076545825128264	35.0	33.0	39.0	30.0	40.0
24	34.50559263689406	35.0	33.0	38.0	27.0	40.0
25	33.528022027633966	35.0	33.0	38.0	24.0	40.0
26	33.49315745314613	35.0	32.0	38.0	24.0	40.0
27	34.28252429055943	35.0	33.0	38.0	26.0	40.0
28	33.989566498354925	35.0	33.0	39.0	24.0	40.0
29	33.98952794593733	35.0	33.0	39.0	24.0	40.0
30	33.16564350579453	35.0	33.0	39.0	21.0	40.0
31	33.05255404693858	35.0	33.0	39.0	21.0	40.0
32	32.26004214387965	35.0	31.0	39.0	15.0	40.0
33	31.384772403773674	35.0	30.0	39.0	10.0	40.0
34	30.671217881017093	35.0	25.0	39.0	8.0	40.0
35	30.148244191013024	35.0	22.0	40.0	7.0	41.0
36	29.446330469294523	35.0	20.0	40.0	7.0	41.0
37	29.421981573973465	35.0	18.0	40.0	7.0	41.0
38	29.1601264519297	35.0	18.0	39.0	7.0	40.0
39	28.85469593809699	35.0	18.0	39.0	7.0	40.0
40	28.632378349368096	35.0	18.0	39.0	7.0	40.0
41	28.274297407552826	35.0	17.0	39.0	7.0	40.0
42	28.458176206868213	35.0	17.0	39.0	7.0	40.0
43	28.294810337323508	35.0	15.0	39.0	7.0	40.0
44	28.369266215603382	35.0	15.0	39.0	7.0	40.0
45	28.510673439717714	35.0	17.0	39.0	7.0	40.0
46	28.3342920710866	35.0	17.0	39.0	7.0	40.0
47	28.229511672758964	35.0	15.0	39.0	7.0	40.0
48	28.06949986354473	35.0	15.0	38.0	7.0	40.0
49	28.20258077999657	35.0	15.0	39.0	7.0	40.0
50	28.175398281982527	35.0	15.0	39.0	7.0	40.0
51	27.09101718524741	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	47.0
10	57.0
11	56.0
12	71.0
13	133.0
14	191.0
15	278.0
16	570.0
17	1202.0
18	2326.0
19	4332.0
20	7111.0
21	10505.0
22	15448.0
23	22576.0
24	33057.0
25	47355.0
26	58357.0
27	56326.0
28	47573.0
29	40532.0
30	39803.0
31	43545.0
32	49913.0
33	59636.0
34	69129.0
35	82728.0
36	95073.0
37	102787.0
38	82637.0
39	12304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.298502238576564	2.383046675817793	30.02807224723057	42.29037883837508
2	41.47702428092132	14.960468553908962	30.5825168844371	12.979990280732617
3	11.413950496666738	15.19969645043833	60.1844834635492	13.201869589345735
4	10.077804865923824	2.650478709427385	72.38520764027754	14.886508784371255
5	19.36832878313352	3.150036878431038	61.588197278808046	15.893437059627402
6	12.33616490695171	13.081849826159035	64.71185618730793	9.87012907958132
7	49.649730995433565	1.0633365494165903	47.05637073628016	2.2305617188696836
8	48.8615369631449	9.54182480766909	38.621913397066564	2.9747248321194393
9	45.85191204773195	2.6082739575375555	37.84812579450953	13.691688200220966
10	25.84422185495972	14.876667772512327	46.240987104216316	13.038123268311638
11	21.851307383498145	15.838347683963514	48.83647789171032	13.473867040828026
12	15.110315713863956	14.430271358292982	53.31210921291182	17.14730371493125
13	15.71325523425159	14.947178115212886	58.39128877688397	10.948277873651554
14	14.274945696890748	20.22398954620761	51.34542864708407	14.155636109817577
15	11.744791111841577	15.4919846480215	59.112929161961745	13.650295078175173
16	13.107517619976644	16.804998828209413	50.66781918104519	19.419664370768746
17	13.793344838186371	17.42508402905229	54.49769750758621	14.283873625175133
18	14.099430743118141	15.974498590300415	53.14460910384905	16.781461562732392
19	13.835752497537209	18.182537580998122	50.44320062170846	17.538509299756207
20	15.80304178574798	17.449737285564858	55.08521606093717	11.662004867749989
21	15.781837956072565	20.972312262408042	51.33508036657262	11.910769414946772
22	14.083705414889957	14.300714944438864	53.86665530384885	17.748924336822327
23	14.145287829306127	20.459463654708316	52.499059016649575	12.896189499335986
24	15.349949425315343	16.836145123474264	50.826188454362565	16.987716996847833
25	12.854492016098678	23.7513328483845	49.04993654069157	14.344238594825251
26	13.580089096666129	15.79675165445671	53.567671160052384	17.05548808882477
27	16.63425219977051	17.710879187883176	49.52271092484206	16.132157687504247
28	12.201028537919854	17.982572278173954	53.72908404528489	16.087315138621307
29	16.411053992660836	15.698341535867444	51.98265952838219	15.90794494308953
30	16.161173454428507	19.150913438662595	50.75385194451293	13.934061162395972
31	15.672775195780336	16.04541474792299	47.72200866211951	20.559801394177164
32	17.307093340475678	19.210771139660192	46.5889733998464	16.893162120017735
33	15.427561529151207	17.420822972371106	46.041833431236185	21.109782067241504
34	17.507768819413375	19.690850192407	46.69763034521661	16.103750642963018
35	20.486145985830973	21.04809819909483	40.039120558482495	18.4266352565917
36	16.7563010375673	20.90870077338179	42.33065596938532	20.00434221966559
37	19.24810611248581	22.258542657742797	39.43506504705931	19.05828618271208
38	19.06234433193226	22.609674019018517	38.27189802682639	20.056083622222832
39	19.40728701564721	21.656516220929703	39.4648924438276	19.471304319595482
40	21.59442653786101	22.022764188050576	39.596173571100294	16.786635702988118
41	18.46508622045287	24.003039553765912	39.7554559279922	17.776418297789018
42	20.322501118527377	22.884207813763418	39.189749926699676	17.603541141009526
43	20.01641521359561	21.492364084973588	38.75948465563053	19.73173604580027
44	21.162132192181772	22.47707399324927	35.89757637183198	20.463217442736976
45	18.377125836105556	21.904164777090937	36.335653580149966	23.383055806653537
46	21.996487671849938	23.449203638942407	34.92463509629481	19.629673592912848
47	16.96529572240636	21.654690053780623	42.66991724419203	18.71009697962099
48	19.284528001736888	23.669053872945437	35.231532631070614	21.814885494247065
49	18.66839949638368	21.47633439555389	39.3170743584827	20.538191749579727
50	18.968803992407203	21.055098506499633	38.05722193307909	21.918875568014073
51	18.860349954498002	21.44336193313996	35.66149354094825	24.03479457141379
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	1235.5
2	2391.0
3	30772.5
4	59154.0
5	45293.0
6	31432.0
7	30388.5
8	29345.0
9	29061.0
10	28777.0
11	28232.5
12	27688.0
13	26757.5
14	25827.0
15	24553.0
16	23279.0
17	21988.0
18	20697.0
19	19480.5
20	18264.0
21	17139.5
22	16015.0
23	15262.0
24	14509.0
25	13996.0
26	13825.0
27	14167.0
28	14351.0
29	14535.0
30	15721.5
31	16908.0
32	17871.5
33	18835.0
34	20830.0
35	22825.0
36	24186.0
37	25547.0
38	26899.5
39	28252.0
40	29761.0
41	31270.0
42	33553.5
43	35837.0
44	39114.5
45	42392.0
46	88613.5
47	134835.0
48	97276.5
49	59718.0
50	55732.5
51	51747.0
52	46058.5
53	40370.0
54	37207.5
55	34045.0
56	31506.5
57	28968.0
58	25970.5
59	22973.0
60	20005.5
61	17038.0
62	14435.5
63	11833.0
64	10018.5
65	8204.0
66	6631.5
67	5059.0
68	4243.0
69	3427.0
70	2849.5
71	2272.0
72	1845.5
73	1419.0
74	1214.0
75	823.5
76	638.0
77	481.5
78	325.0
79	233.5
80	142.0
81	107.0
82	72.0
83	58.0
84	44.0
85	25.5
86	7.0
87	8.5
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	985671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.78974105638283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23380293295236	38.944880863708384
2	5.3402266487366825	4.99735644153569
3	2.486294607542159	3.4899924263033575
4	1.6794924555468165	3.1433206840473633
5	1.3188645138754023	3.0854664546341146
6	1.0823392051882914	3.0385422687558816
7	0.9141642181737797	2.994145493595327
8	0.7458372561824459	2.791802566958377
9	0.6303733952116667	2.6545507137708086
>10	2.478831805730803	16.495443117500646
>50	0.04960638615851403	1.6287418222954901
>100	0.034679337729266076	3.1066314073741075
>500	0.0028533632252977052	0.8915261890194932
>1k	0.00153642635208338	2.5517510907231133
>5k	2.1948947886905429E-4	0.6057160998545065
>10k+	8.779579154762171E-4	9.580132359923327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28257	2.8667780628627604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	23283	2.3621472073338876	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	22714	2.304420034676885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	19030	1.9306644914986848	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT	5898	0.5983741025149365	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCTTGTT	4878	0.49489129740045107	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTC	4640	0.47074530954040444	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	4278	0.4340190590978126	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCT	3787	0.38420527742015337	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTA	3640	0.3692915790360069	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTT	2015	0.204429266966361	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGT	1609	0.1632390523815756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0145373050439751E-4	0.0	0.0	0.05945188607557694	0.0
2	1.0145373050439751E-4	0.0	0.0	0.5602274998452831	0.0
3	1.0145373050439751E-4	0.0	0.0	0.6498111438806661	0.0
4	1.0145373050439751E-4	0.0	0.0	1.369219546887349	0.0
5	1.0145373050439751E-4	0.0	0.0	4.156153523843148	0.0
6	1.0145373050439751E-4	0.0	0.0	4.4601089004343235	0.0
7	1.0145373050439751E-4	0.0	0.0	4.960985967934534	0.0
8	1.0145373050439751E-4	0.0	0.0	5.571128703187981	0.0
9	1.0145373050439751E-4	0.0	0.0	5.670756266543299	0.0
10	1.0145373050439751E-4	0.0	0.0	8.525461335476036	0.0
11	1.0145373050439751E-4	0.0	0.0	8.666684928338157	0.0
12	1.0145373050439751E-4	0.0	0.0	11.027208875983975	0.0
13	1.0145373050439751E-4	0.0	0.0	11.207695062551297	0.0
14	1.0145373050439751E-4	0.0	0.0	11.359165482194364	0.0
15	1.0145373050439751E-4	0.0	0.0	11.845636119962949	0.0
16	1.0145373050439751E-4	0.0	0.0	12.102009696947562	0.0
17	1.0145373050439751E-4	0.0	0.0	12.224870164588387	0.0
18	1.0145373050439751E-4	0.0	0.0	12.32713552493682	0.0
19	1.0145373050439751E-4	0.0	0.0	13.026557543034135	0.0
20	1.0145373050439751E-4	0.0	0.0	13.166462237399701	0.0
21	1.0145373050439751E-4	0.0	0.0	13.26010403065526	0.0
22	1.0145373050439751E-4	0.0	0.0	13.477722282587193	0.0
23	1.0145373050439751E-4	0.0	0.0	13.585364690652357	0.0
24	1.0145373050439751E-4	0.0	0.0	13.684586439085658	0.0
25	1.0145373050439751E-4	0.0	0.0	13.760169468311435	0.0
26	1.0145373050439751E-4	0.0	0.0	13.876435443469473	0.0
27	1.0145373050439751E-4	0.0	0.0	14.025065158658416	0.0
28	1.0145373050439751E-4	0.0	0.0	14.113329904197242	0.0
29	1.0145373050439751E-4	0.0	0.0	14.214276366049118	0.0
30	1.0145373050439751E-4	0.0	0.0	14.329324896441104	0.0
31	1.0145373050439751E-4	0.0	0.0	14.432604794094582	0.0
32	1.0145373050439751E-4	0.0	0.0	14.555668169196416	0.0
33	1.0145373050439751E-4	0.0	0.0	14.671021060779916	0.0
34	1.0145373050439751E-4	0.0	0.0	14.833245575856447	0.0
35	1.0145373050439751E-4	0.0	0.0	14.968483398618808	0.0
36	1.0145373050439751E-4	0.0	0.0	15.091546773720642	0.0
37	1.0145373050439751E-4	0.0	0.0	15.231350014355703	0.0
38	1.0145373050439751E-4	0.0	0.0	15.375211404210939	0.0
39	1.0145373050439751E-4	0.0	0.0	15.575988336879142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGCGT	25	3.8903996E-5	45.0	44
CGTTTTT	29345	0.0	44.486282	1
CCGATGA	725	0.0	42.827583	18
GATACCT	2735	0.0	42.367462	5
CGATGAA	705	0.0	42.127663	19
TGATACC	2765	0.0	41.8264	4
ACGGCTG	2315	0.0	41.695465	8
TACGGCT	2310	0.0	41.68831	7
CGGCTGT	2360	0.0	41.472458	9
ATACCTG	2815	0.0	41.16341	6
CGACCAC	2245	0.0	40.389755	12
ATGATAC	8865	0.0	40.02538	3
GAATGAT	9270	0.0	39.660194	1
GCGACCA	2330	0.0	39.592274	11
ATACGGC	6155	0.0	39.406986	6
AATGATA	8810	0.0	39.381382	2
GCGATCG	40	3.4575532E-7	39.375004	9
GAATCTG	2985	0.0	39.346733	1
TACCTGT	2965	0.0	39.3086	7
ACCACCG	1420	0.0	39.137325	14
>>END_MODULE