##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933411.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664216
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.736368892047164	31.0	28.0	31.0	16.0	33.0
2	29.110019933274717	31.0	30.0	33.0	16.0	34.0
3	29.270890794560806	31.0	30.0	33.0	16.0	34.0
4	32.271187384826625	35.0	32.0	37.0	19.0	37.0
5	33.73055000180664	35.0	33.0	37.0	28.0	37.0
6	33.01011417972467	35.0	33.0	37.0	25.0	37.0
7	32.28062407409638	35.0	32.0	35.0	25.0	37.0
8	33.1458019680345	35.0	33.0	37.0	28.0	37.0
9	33.14945439435365	35.0	31.0	37.0	25.0	39.0
10	32.19667246799234	35.0	28.0	39.0	15.0	39.0
11	32.831509629397665	37.0	30.0	39.0	17.0	39.0
12	33.6271197923567	37.0	31.0	39.0	25.0	39.0
13	33.84190082744168	37.0	32.0	39.0	26.0	39.0
14	33.90804196225324	37.0	32.0	40.0	21.0	41.0
15	34.61792850518506	37.0	32.0	40.0	25.0	41.0
16	34.7852791862888	37.0	32.0	40.0	25.0	41.0
17	32.83546617365435	36.0	30.0	40.0	18.0	41.0
18	33.432360858515906	36.0	31.0	39.0	24.0	39.0
19	33.16771050381201	37.0	32.0	37.0	23.0	39.0
20	32.63734688715719	35.0	31.0	36.0	25.0	39.0
21	32.86822208438219	35.0	32.0	37.0	24.0	39.0
22	32.876496501138185	34.0	30.0	37.0	25.0	39.0
23	33.9331738470618	35.0	33.0	38.0	27.0	40.0
24	32.13615600949089	35.0	31.0	37.0	21.0	39.0
25	31.350172233128983	34.0	29.0	37.0	18.0	39.0
26	30.34058348489046	33.0	25.0	37.0	18.0	39.0
27	32.251588338733185	34.0	30.0	37.0	22.0	39.0
28	32.295708022691414	35.0	31.0	37.0	21.0	39.0
29	32.40757976320956	35.0	31.0	38.0	21.0	40.0
30	31.39666162814506	35.0	29.0	37.0	18.0	40.0
31	31.67011183109109	35.0	30.0	37.0	18.0	40.0
32	31.350024690763245	35.0	30.0	37.0	16.0	40.0
33	30.901768400640755	35.0	29.0	38.0	12.0	40.0
34	29.72752839437773	35.0	24.0	38.0	8.0	40.0
35	29.05711093981476	35.0	22.0	38.0	7.0	40.0
36	28.342766509689618	34.0	19.0	38.0	7.0	40.0
37	28.453427800594987	34.0	18.0	38.0	7.0	40.0
38	28.386752201091213	34.0	18.0	38.0	7.0	40.0
39	28.027750009033205	34.0	18.0	38.0	7.0	40.0
40	27.829221217194405	33.0	18.0	38.0	7.0	40.0
41	27.43114438676575	33.0	17.0	38.0	7.0	40.0
42	27.81889776819589	33.0	18.0	38.0	7.0	40.0
43	27.70856167270888	33.0	16.0	38.0	7.0	40.0
44	27.681907692678287	34.0	16.0	38.0	7.0	40.0
45	27.92877768677659	34.0	17.0	38.0	7.0	40.0
46	27.713421537572113	34.0	17.0	38.0	7.0	40.0
47	27.7263977380852	34.0	15.0	38.0	7.0	40.0
48	27.46604116733111	33.0	15.0	38.0	7.0	40.0
49	27.25196622785359	33.0	15.0	37.0	7.0	40.0
50	27.408556854998977	33.0	15.0	38.0	7.0	40.0
51	26.072723330964624	31.0	15.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	19.0
10	26.0
11	48.0
12	63.0
13	72.0
14	174.0
15	360.0
16	709.0
17	1468.0
18	2632.0
19	4378.0
20	6781.0
21	9777.0
22	13427.0
23	18589.0
24	25632.0
25	34389.0
26	41054.0
27	40384.0
28	35840.0
29	34195.0
30	36563.0
31	41766.0
32	48007.0
33	54935.0
34	61877.0
35	65239.0
36	51235.0
37	28435.0
38	6018.0
39	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.614378455201322	2.4547737482987464	32.466396473436355	44.46445132306358
2	45.07825767521409	9.8475495923013	32.30274489021643	12.771447842268177
3	12.198592024281258	9.885187950907536	64.88461584785672	13.031604176954485
4	10.649397184048562	2.773344815541932	71.12114131547568	15.456116684933816
5	16.44284389415491	3.222596263865971	64.8295132908572	15.505046551121923
6	12.682771869391885	9.045099786816337	67.24830476832837	11.023823575463403
7	50.43178724993075	1.1377323039493177	46.05083286159924	2.3796475845206984
8	51.452238428463026	6.414931287412529	39.14554903826466	2.9872812458597804
9	47.583316270610766	2.62655521697761	39.25108699579655	10.539041516615077
10	24.358341262480877	16.000216796945573	46.961681139870166	12.679760800703384
11	20.189065002950848	16.37253544027846	50.7030845387645	12.73531501800619
12	15.070097679068256	14.934298481216954	54.573813337829854	15.42179050188493
13	15.0860563431173	15.239921953099595	58.4443915834608	11.229630120322305
14	13.991833981716791	17.441916484998856	54.0381442181459	14.528105315138449
15	11.984354487094558	15.733135004275717	58.81339805123634	13.46911245739338
16	13.370801064713888	15.413510062991556	53.098088573596534	18.117600298698015
17	14.096016958338852	15.915154106495477	55.62588073759138	14.362948197574282
18	14.123116576535343	14.691154684620667	54.90036373709757	16.285365001746417
19	14.283305430763487	17.20072988305009	52.32650222216869	16.18946246401773
20	15.717477447095524	16.72769099208691	55.5951678369687	11.959663723848868
21	16.21987425777157	18.636256880291953	52.45296710708565	12.690901754850831
22	14.399081021836272	14.748063882833296	54.559962421862764	16.29289267346767
23	13.976176424536597	19.26828019800788	53.21010032880869	13.545443048646828
24	14.35828104110711	16.411528779794523	53.03470557770363	16.19548460139473
25	13.287093355173617	19.751556722511953	51.36506798993099	15.596281932383441
26	13.442313946065736	16.66280246184976	54.37749165933974	15.51739193274477
27	14.811446878726198	17.228883375287555	52.1822720319896	15.777397713996653
28	12.433605935418598	17.433334939236634	54.76516675298397	15.367892372360798
29	15.498572753441653	15.737802160742891	53.207390366989046	15.556234718826406
30	14.964258614667516	17.6654883351199	52.96334324978621	14.406909800426368
31	15.052633480674963	16.187204162501356	50.67688824117455	18.083274115649125
32	15.996302407650523	18.41449167138401	49.83830561142761	15.75090030953786
33	14.896359015741867	17.276909920869116	48.65164344129018	19.175087622098836
34	16.391354619581584	19.55839666614475	47.767443120912475	16.282805593361196
35	17.98481216953521	21.09434280414805	42.71065436544739	18.210190660869355
36	16.669878473267733	20.443500307129007	43.19874859985306	19.687872619750202
37	18.275681404844207	21.980951979476558	40.902658171438205	18.84070844424103
38	18.249635660688693	22.144754116130898	40.98094595733917	18.624664265841233
39	18.31768581304877	21.360220169342504	41.6238091223337	18.69828489527503
40	20.703807195249738	21.37617883339155	41.287322196393944	16.63269177496477
41	18.154786997000976	22.520384935021138	41.899773567634625	17.42505450034326
42	19.392185674539608	22.415449191226948	40.7239512447758	17.468413889457647
43	18.817222108470737	21.262360436966286	41.03936068989606	18.881056764666916
44	19.98882893516567	22.30795403904754	38.381490358558054	19.321726667228734
45	18.01100846712515	21.798180110084672	38.580371445433414	21.610439977356762
46	19.78242017656907	22.67906825490503	37.727937899719365	19.810573668806533
47	17.11732328037867	21.265371505654787	42.86783215098703	18.74947306297951
48	18.454087224637767	22.457905259734783	38.39353463331206	20.69447288231539
49	18.006040203789127	21.231045322605897	40.045708022691414	20.717206450913558
50	17.987672684789285	20.66586772977465	39.95537596203644	21.391083623399616
51	18.341924915991186	20.970587881050744	38.27926457658352	22.408222626374553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	461.0
2	844.0
3	27626.5
4	54409.0
5	38580.5
6	22752.0
7	21845.0
8	20938.0
9	20764.0
10	20590.0
11	19994.5
12	19399.0
13	18533.5
14	17668.0
15	16743.0
16	15818.0
17	14793.5
18	13769.0
19	12774.5
20	11780.0
21	10997.0
22	10214.0
23	9585.0
24	8956.0
25	8689.5
26	8663.0
27	8903.0
28	9028.5
29	9154.0
30	10092.0
31	11030.0
32	11757.0
33	12484.0
34	13792.5
35	15101.0
36	16295.0
37	17489.0
38	18558.0
39	19627.0
40	20651.5
41	21676.0
42	23305.5
43	24935.0
44	26712.0
45	28489.0
46	45487.5
47	62486.0
48	53114.0
49	43742.0
50	40890.0
51	38038.0
52	33590.0
53	29142.0
54	26190.0
55	23238.0
56	21352.0
57	19466.0
58	17398.0
59	15330.0
60	13620.5
61	11911.0
62	10093.5
63	8276.0
64	7116.5
65	5957.0
66	4996.0
67	4035.0
68	3430.0
69	2825.0
70	2404.0
71	1983.0
72	1630.0
73	1277.0
74	1078.5
75	707.5
76	535.0
77	434.0
78	333.0
79	220.5
80	108.0
81	89.0
82	70.0
83	51.5
84	33.0
85	24.0
86	15.0
87	9.5
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	664216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.11922251391473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63538621962869	40.0986670073131
2	6.5369173851627815	6.421765991937765
3	3.0484876956180518	4.492180363559826
4	2.058979750630722	4.045419380915003
5	1.4665882980933453	3.6018838475175263
6	1.0939577707791215	3.2240613098235475
7	0.8753790564732624	3.009855706125163
8	0.6417263292130476	2.5216878686122706
9	0.5173242194884472	2.2869507104001268
>10	2.041080528593153	14.365356585834627
>50	0.04616938103757573	1.6485547056869925
>100	0.03297812689743196	3.2031573531054938
>500	0.002198541793162084	0.713509741275959
>1k	0.001570386995115774	1.3309077919973402
>5k	6.281547980463096E-4	2.071878016288893
>10k+	6.281547980463096E-4	6.964163619606343
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35080	5.281414479627109	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	10062	1.5148686571838077	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	7091	1.0675744035072927	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	6339	0.9543582208197333	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCTTGTT	2202	0.3315186626037313	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCT	2129	0.32052826189071026	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	1849	0.27837330025172535	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTC	1329	0.20008551435075336	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTA	1118	0.16831873968708974	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCT	862	0.12977706047430354	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGCCTTGTTCGTATGCCG	858	0.12917484673660376	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07768557216327218	0.0
2	0.0	0.0	0.0	0.4852337191516013	0.0
3	0.0	0.0	0.0	0.6178712948799788	0.0
4	0.0	0.0	0.0	1.1538415214327868	0.0
5	0.0	0.0	0.0	2.4731412672985895	0.0
6	0.0	0.0	0.0	2.7885507124188518	0.0
7	0.0	0.0	0.0	3.114649451383285	0.0
8	0.0	0.0	0.0	3.5518566249533285	0.0
9	0.0	0.0	0.0	3.710238837968372	0.0
10	0.0	0.0	0.0	5.7586688667541885	0.0
11	0.0	0.0	0.0	6.155377166463921	0.0
12	0.0	0.0	0.0	7.702313705180242	0.0
13	0.0	0.0	0.0	7.880418418104954	0.0
14	0.0	0.0	0.0	7.999054524431811	0.0
15	0.0	0.0	0.0	8.329368759560143	0.0
16	0.0	0.0	0.0	8.534573090681345	0.0
17	0.0	0.0	0.0	8.740680742409095	0.0
18	0.0	0.0	0.0	8.933539691907452	0.0
19	0.0	0.0	0.0	9.51091211292712	0.0
20	0.0	0.0	0.0	9.707986558589374	0.0
21	0.0	0.0	0.0	9.919514133956424	0.0
22	0.0	0.0	0.0	10.199694075421249	0.0
23	0.0	0.0	0.0	10.41950208968167	0.0
24	0.0	0.0	0.0	10.602575065942403	0.0
25	0.0	0.0	0.0	10.751924072891951	0.0
26	0.0	0.0	0.0	10.896154263071049	0.0
27	0.0	0.0	0.0	11.048213231840245	0.0
28	0.0	0.0	0.0	11.189281799896419	0.0
29	0.0	0.0	0.0	11.35669721897696	0.0
30	0.0	0.0	0.0	11.531640309778746	0.0
31	0.0	0.0	0.0	11.689269755621666	0.0
32	0.0	0.0	0.0	11.85954568995628	0.0
33	0.0	0.0	0.0	12.032230479241692	0.0
34	0.0	0.0	0.0	12.20627024943693	0.0
35	0.0	0.0	0.0	12.382718874582967	0.0
36	0.0	0.0	0.0	12.55510255699953	0.0
37	0.0	0.0	0.0	12.730798414973442	0.0
38	0.0	0.0	0.0	12.91914076143905	0.0
39	0.0	0.0	0.0	13.159424042781264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	25	3.8890204E-5	45.000004	7
GCGAGCC	25	3.8890204E-5	45.000004	33
CGTTTTT	22235	0.0	44.50416	1
ACCGGTG	105	0.0	42.85714	17
CGACCAC	1050	0.0	41.999996	12
CACCGGT	145	0.0	41.896553	16
GACCGCC	70	0.0	41.785717	13
CCGATGA	195	0.0	41.53846	18
GCGACCA	1060	0.0	41.39151	11
GAATGAT	4105	0.0	41.053593	1
CGGCGAC	1630	0.0	40.996933	9
ACGGCGA	1590	0.0	40.896225	8
TACGGCT	875	0.0	40.885715	7
ATACGGC	2465	0.0	40.80122	6
ATGATAC	3850	0.0	40.79221	3
TACGGCG	1665	0.0	40.67568	7
ACGGCTG	880	0.0	40.653408	8
TGATACC	1395	0.0	40.64516	4
GGCGACC	1480	0.0	40.591213	10
AATGATA	3830	0.0	40.535248	2
>>END_MODULE