##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933408.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621560
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.149472295514514	31.0	28.0	31.0	25.0	34.0
2	28.09782643670764	31.0	25.0	33.0	16.0	34.0
3	28.11056856940601	31.0	25.0	33.0	16.0	34.0
4	32.815390308256646	35.0	32.0	37.0	28.0	37.0
5	34.07671182186756	35.0	33.0	37.0	30.0	37.0
6	33.23856425767424	35.0	32.0	37.0	28.0	37.0
7	33.341865628418816	35.0	32.0	36.0	28.0	37.0
8	33.13974033078062	35.0	32.0	37.0	28.0	37.0
9	34.41775854302079	37.0	34.0	39.0	28.0	39.0
10	34.334426282257546	37.0	32.0	39.0	27.0	39.0
11	34.534950769032754	37.0	33.0	39.0	27.0	39.0
12	34.65026546109788	37.0	33.0	39.0	27.0	39.0
13	34.72465409614518	37.0	33.0	39.0	27.0	39.0
14	35.58386800952442	38.0	34.0	40.0	27.0	41.0
15	35.739243194542766	38.0	34.0	40.0	27.0	41.0
16	35.74143123753137	37.0	34.0	40.0	27.0	41.0
17	35.01094986807388	37.0	33.0	40.0	27.0	41.0
18	34.84138940729777	37.0	33.0	39.0	27.0	40.0
19	34.2072865049231	37.0	32.0	38.0	27.0	40.0
20	33.5628016603385	35.0	32.0	38.0	25.0	40.0
21	34.23471426732737	35.0	33.0	38.0	27.0	40.0
22	34.86878981916468	35.0	33.0	39.0	29.0	40.0
23	35.09133953278847	35.0	33.0	39.0	29.0	40.0
24	34.707128837119505	35.0	33.0	39.0	27.0	40.0
25	33.717293583885706	35.0	33.0	38.0	24.0	40.0
26	33.77590095887766	35.0	32.0	38.0	25.0	40.0
27	34.424023424930816	35.0	33.0	39.0	27.0	40.0
28	34.05648851277431	35.0	33.0	39.0	25.0	40.0
29	34.01024036295772	35.0	33.0	39.0	24.0	40.0
30	33.51533560718193	35.0	33.0	39.0	22.0	40.0
31	33.586643284638654	35.0	33.0	39.0	23.0	40.0
32	33.073297831263275	35.0	32.0	39.0	20.0	40.0
33	32.59817716712787	35.0	31.0	39.0	15.0	40.0
34	32.320862024583306	36.0	31.0	39.0	12.0	40.0
35	31.991329879657634	36.0	31.0	40.0	10.0	41.0
36	31.687846708282386	36.0	30.0	40.0	9.0	41.0
37	31.681737885320807	36.0	30.0	40.0	8.0	40.0
38	31.34955112941631	35.0	29.0	39.0	8.0	40.0
39	31.06855975287985	35.0	28.0	39.0	8.0	40.0
40	30.671061522620505	35.0	26.0	39.0	8.0	40.0
41	30.19017311281292	35.0	24.0	39.0	8.0	40.0
42	30.56803204839436	35.0	26.0	39.0	7.0	40.0
43	30.416810927344102	35.0	25.0	39.0	7.0	40.0
44	30.650316944462322	35.0	26.0	39.0	7.0	40.0
45	30.764606795804106	35.0	26.0	39.0	7.0	40.0
46	30.51970847544887	35.0	26.0	39.0	7.0	40.0
47	30.386649720059207	35.0	25.0	39.0	7.0	40.0
48	30.27440150588841	35.0	25.0	39.0	7.0	40.0
49	30.54661657764335	35.0	27.0	39.0	7.0	40.0
50	30.45370197567411	35.0	26.0	39.0	7.0	40.0
51	29.170509685307934	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	21.0
9	30.0
10	46.0
11	38.0
12	53.0
13	67.0
14	108.0
15	145.0
16	300.0
17	570.0
18	1106.0
19	2126.0
20	3404.0
21	5040.0
22	7074.0
23	9918.0
24	13989.0
25	19893.0
26	25762.0
27	28178.0
28	27823.0
29	27020.0
30	29051.0
31	33588.0
32	39505.0
33	46691.0
34	53339.0
35	61377.0
36	66323.0
37	64490.0
38	47626.0
39	6859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98233477057726	2.5011261985970785	30.616352403629577	33.900186627196085
2	32.38448420104254	21.39889954308514	31.918237981852116	14.298378274020207
3	12.562906235922517	21.72195765493275	52.25191453761503	13.463221571529699
4	11.125876826050582	2.9823347705772574	69.27891112684213	16.61287727653002
5	25.547171632666192	3.586298989638973	52.515445009331366	18.351084368363473
6	13.96647145890984	18.811860480082373	55.88438766973422	11.33728039127357
7	53.0705000321771	1.5359740009009588	42.526224338760535	2.8673016281614
8	51.01567024905077	14.726172855396099	30.141096595662525	4.117060299890598
9	47.861831520689876	3.082244674689491	28.938477379496753	20.11744642512388
10	31.47161979535363	18.745253877340883	35.49456206963125	14.288564257674238
11	27.584464894780876	15.615387090546367	41.64795031855332	15.15219769611944
12	20.29329429178197	14.307548748310703	44.42242100521269	20.97673595469464
13	19.0657378209666	15.402535555698565	52.57481176394877	12.956914859386062
14	16.26713430722698	22.50386125233284	42.65702426153549	18.57198017890469
15	12.28698757963833	15.223791749790848	54.77266876890404	17.716551901666776
16	14.60084304009267	17.86408391788403	41.84230001930626	25.692773022717034
17	15.085108436836347	18.75152841238175	48.37425188236051	17.789111268421394
18	15.651264560139005	16.854688203874126	46.04511229808868	21.448934937898194
19	15.232801338567475	20.089935002252396	42.378370551515545	22.298893107664586
20	18.374090996846643	19.031469206512647	49.33473840015445	13.259701396486259
21	18.062616641997558	25.34686916790012	42.271864341334705	14.318649848767617
22	15.51676427054508	15.25162494369007	47.06367848638909	22.167932299375764
23	16.543696505566636	24.176909711049618	44.543406911641675	14.735986871742067
24	18.593377952249178	17.560171182186757	42.0528991569599	21.79355170860416
25	14.452828367333806	28.45163781453118	39.69431752365017	17.401216294484843
26	15.424576871098527	16.43735118089967	45.742647532016214	22.395424415985584
27	18.39371902953858	19.443336122015573	40.73074200398996	21.432202844455887
28	12.15473968723856	20.946167707059658	46.77311924834288	20.1259733573589
29	18.212240169895104	19.8196473389536	40.609756097560975	21.358356393590324
30	14.989381556084691	22.00189844906365	43.54688203874123	19.461837956110433
31	19.321706673531118	16.601937061586973	37.94018276594375	26.136173498938152
32	20.39336508140807	21.908423965506145	38.255196602097946	19.443014350987838
33	16.581826372353433	17.82595405109724	38.28866078898256	27.303558787566768
34	19.24222922968016	23.33563935903211	36.38120213655963	21.040929274728104
35	15.496975352339277	21.651328914344553	38.46772636591801	24.38396936739816
36	16.83747345389021	29.1109466503636	31.814788596434777	22.23679129931141
37	16.84535684406976	21.554475834995817	35.328689104833	26.271478216101425
38	18.41205997811957	22.919589420168606	34.61274856811893	24.055602033592898
39	19.90362957719287	23.050711113971296	30.82325117446425	26.222408134371584
40	18.51036102709312	20.621661625587233	41.448291395842716	19.41968595147693
41	17.621951219512194	28.289626102065768	29.60277366625909	24.485649012162945
42	17.915728167835766	21.432524615483622	40.62648819100328	20.02525902567733
43	20.085108436836347	25.28074522170024	31.219029538580344	23.41511680288307
44	21.334867108565543	20.595437286826694	33.637299697535234	24.432395907072525
45	18.12922324473904	19.933071626230774	34.22227942596049	27.715425703069695
46	24.143445524165006	24.698017890469142	30.441148079026963	20.71738850633889
47	15.614582662977025	21.12088937512066	43.41318617671665	19.85134178518566
48	20.12516892978956	23.850473003410773	31.39519917626617	24.629158890533496
49	18.01579895746187	19.559495463028508	40.3352854109016	22.08942016860802
50	19.664231932556792	20.781742711886224	35.90610721410644	23.647918141450543
51	19.097271381684795	20.12532981530343	33.14708153677843	27.63031726623335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	848.5
2	1640.0
3	17448.0
4	33256.0
5	24680.0
6	16104.0
7	15132.0
8	14160.0
9	13896.5
10	13633.0
11	12921.5
12	12210.0
13	11457.5
14	10705.0
15	9864.5
16	9024.0
17	8422.0
18	7820.0
19	7167.5
20	6515.0
21	6234.0
22	5953.0
23	5713.5
24	5474.0
25	5339.5
26	5525.0
27	5845.0
28	6117.0
29	6389.0
30	7216.0
31	8043.0
32	8775.5
33	9508.0
34	10667.0
35	11826.0
36	12743.0
37	13660.0
38	14253.0
39	14846.0
40	15751.0
41	16656.0
42	17963.0
43	19270.0
44	21944.0
45	24618.0
46	68653.5
47	112689.0
48	76501.0
49	40313.0
50	37817.0
51	35321.0
52	31195.0
53	27069.0
54	26638.0
55	26207.0
56	25834.5
57	25462.0
58	24004.0
59	22546.0
60	20091.5
61	17637.0
62	15396.5
63	13156.0
64	11261.0
65	9366.0
66	8042.5
67	6719.0
68	5535.5
69	4352.0
70	3695.5
71	3039.0
72	2592.0
73	2145.0
74	1791.0
75	1151.5
76	866.0
77	660.0
78	454.0
79	330.0
80	206.0
81	150.5
82	95.0
83	67.0
84	39.0
85	25.0
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	621560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.48689695039954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61812438567814	33.449514882382424
2	5.460573617449286	4.421633626794794
3	2.208122688544156	2.6820010723477927
4	1.496370081076338	2.4233352508879484
5	1.1659366458542149	2.360257841569704
6	1.0307007875730332	2.5037925943899024
7	0.8769827629405506	2.485441752531552
8	0.7824610748465786	2.5343536724009827
9	0.6616641180364777	2.4109854266447885
>10	3.5777491715454324	21.84818098493125
>50	0.07069993807422113	2.036222287500322
>100	0.044187461285492995	3.222825945823857
>500	8.034083870089634E-4	0.179063956719422
>1k	0.0040170419350448175	4.811469659934041
>5k	0.0	0.0
>10k+	0.0016068167740179269	12.630921045141207
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	23104	3.717098912413926	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	20513	3.30024454598108	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	19881	3.198564901216294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14165	2.2789433039449127	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	4717	0.7588969689169187	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT	4706	0.7571272282643671	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC	4271	0.687142029731643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA	3856	0.6203745414762855	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	3844	0.6184439153098655	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	3629	0.5838535298281743	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCCTCTATCGT	1440	0.23167513997039707	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	1073	0.1726301563807195	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATG	1037	0.16683827788145955	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTAT	1011	0.16265525452088292	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6088551386833128E-4	0.0	0.0	0.09041765879400218	0.0
2	1.6088551386833128E-4	0.0	0.0	0.8150460132569664	0.0
3	1.6088551386833128E-4	0.0	0.0	0.9279876439925349	0.0
4	1.6088551386833128E-4	0.0	0.0	1.8492180964026	0.0
5	1.6088551386833128E-4	0.0	0.0	5.586427698050068	0.0
6	1.6088551386833128E-4	0.0	0.0	5.869425316944462	0.0
7	1.6088551386833128E-4	0.0	0.0	6.60628097046142	0.0
8	1.6088551386833128E-4	0.0	0.0	7.500160885513869	0.0
9	1.6088551386833128E-4	0.0	0.0	7.626777784928245	0.0
10	1.6088551386833128E-4	0.0	0.0	11.994175944397966	0.0
11	1.6088551386833128E-4	0.0	0.0	12.198822318038484	0.0
12	1.6088551386833128E-4	0.0	0.0	16.174625136752688	0.0
13	1.6088551386833128E-4	0.0	0.0	16.47805521590836	0.0
14	1.6088551386833128E-4	0.0	0.0	16.71133921101744	0.0
15	1.6088551386833128E-4	0.0	0.0	17.52815496492696	0.0
16	1.6088551386833128E-4	0.0	0.0	17.885964347770127	0.0
17	1.6088551386833128E-4	0.0	0.0	18.04749340369393	0.0
18	1.6088551386833128E-4	0.0	0.0	18.162687431623656	0.0
19	1.6088551386833128E-4	0.0	0.0	19.202168736726946	0.0
20	1.6088551386833128E-4	0.0	0.0	19.409228393075487	0.0
21	1.6088551386833128E-4	0.0	0.0	19.539545659308835	0.0
22	1.6088551386833128E-4	0.0	0.0	19.84651521976961	0.0
23	1.6088551386833128E-4	0.0	0.0	19.974258317781068	0.0
24	1.6088551386833128E-4	0.0	0.0	20.09830104897355	0.0
25	1.6088551386833128E-4	0.0	0.0	20.191936418044918	0.0
26	1.6088551386833128E-4	0.0	0.0	20.329493532402342	0.0
27	1.6088551386833128E-4	0.0	0.0	20.544114807902698	0.0
28	1.6088551386833128E-4	0.0	0.0	20.664618057790076	0.0
29	1.6088551386833128E-4	0.0	0.0	20.785121307677457	0.0
30	1.6088551386833128E-4	0.0	0.0	20.933618636977926	0.0
31	1.6088551386833128E-4	0.0	0.0	21.056213398545594	0.0
32	1.6088551386833128E-4	0.0	0.0	21.213237660081088	0.0
33	1.6088551386833128E-4	0.0	0.0	21.356264881910032	0.0
34	1.6088551386833128E-4	0.0	0.0	21.548040414441083	0.0
35	1.6088551386833128E-4	0.0	0.0	21.710534783448097	0.0
36	1.6088551386833128E-4	0.0	0.0	21.852757577707703	0.0
37	1.6088551386833128E-4	0.0	0.0	22.011551579895745	0.0
38	1.6088551386833128E-4	0.0	0.0	22.18562970590128	0.0
39	1.6088551386833128E-4	0.0	0.0	22.437737306132956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	20	7.030784E-4	45.0	27
CGATATC	20	7.030784E-4	45.0	10
CGTTAGG	25	3.8887323E-5	44.999996	2
GCGATAT	25	3.8887323E-5	44.999996	9
CCTTGCG	25	3.8887323E-5	44.999996	1
CGTTTTT	12570	0.0	44.19451	1
GTATGAT	150	0.0	43.500004	1
TGATACC	2665	0.0	42.973736	4
GATACCT	2680	0.0	42.649254	5
ATACCTG	2690	0.0	42.490707	6
CGATGAA	670	0.0	42.313435	19
TACGGCT	2460	0.0	42.07317	7
ACGGCTG	2480	0.0	41.91532	8
CGGCTGT	2475	0.0	41.909092	9
CCGATGA	670	0.0	41.641792	18
TACCTGT	2780	0.0	41.43885	7
GAATCTG	2320	0.0	40.732758	1
GAATGAT	9005	0.0	40.577454	1
ATGATAC	8645	0.0	40.47137	3
AATCTGT	2345	0.0	40.394455	2
>>END_MODULE