FastQCFastQC Report
Sat 14 Jan 2017
SRR2933407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417839
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212165.077553794643391No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG109002.6086602734546083No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC84772.0287718475297902No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC61371.468747531944122No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC23570.564092868305735TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT22860.5471006775336912No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT20240.4843971003185437No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC16070.38459789536161054No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA13960.3340999763066636No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT9930.23765134417802072No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCACCCTCTATCGTATGCCG9150.21898386699183178No Hit
GAATCTTTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC5520.13210830008687557No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT5350.12803974736680876TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCCTCTATCGT5340.12780042073621659No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCACCCTCTATCGTATGC4780.1143981294230553No Hit
GAATGACTCTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT4590.10985092344180412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC406.7975634E-945.017
CGCGGGC453.8380676E-1045.04
ACGTCGG207.028126E-445.043
CCTGATC207.028126E-445.031
CTTACGG207.028126E-445.02
CGTTGAT302.1621727E-644.99999625
CGTTTTT103400.044.3471951
ACGGCTG11400.043.6184238
CGGGATG1500.043.56
CGATGAA1450.043.44827319
TACGGCT11500.043.239137
TGATCCG1100.042.9545444
GAATGAT41600.041.808891
GATACCT13650.041.5384645
ACGGCGA14700.041.479598
ATACGGC26700.041.3764046
ATGATAC40000.041.3437543
GGCGACT2450.041.3265310
TGATACG26850.041.3128474
CGACCAC8400.041.2512
Produced by FastQC (version 0.11.5)