##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933407.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417839
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.07185542756899	31.0	25.0	31.0	16.0	33.0
2	28.151378880382158	31.0	28.0	33.0	16.0	34.0
3	28.353404062330227	31.0	28.0	33.0	16.0	34.0
4	31.52775351271662	35.0	28.0	35.0	19.0	37.0
5	33.45299505311855	35.0	33.0	37.0	28.0	37.0
6	32.09423246752936	35.0	32.0	37.0	17.0	37.0
7	31.76241327401224	33.0	30.0	35.0	25.0	37.0
8	32.40983728182386	35.0	32.0	35.0	25.0	37.0
9	32.590933349926644	34.0	31.0	37.0	25.0	39.0
10	31.279296571167365	35.0	27.0	38.0	15.0	39.0
11	31.86210956851802	35.0	27.0	39.0	15.0	39.0
12	32.76833421485309	35.0	31.0	39.0	22.0	39.0
13	32.95165362735408	35.0	31.0	39.0	23.0	39.0
14	33.20649341014123	36.0	31.0	39.0	19.0	41.0
15	33.63134843803475	36.0	31.0	39.0	23.0	41.0
16	33.85029161949937	36.0	32.0	40.0	24.0	41.0
17	31.754668664246278	34.0	27.0	39.0	17.0	41.0
18	32.541253927948325	35.0	30.0	39.0	20.0	39.0
19	32.36946287924296	35.0	30.0	37.0	18.0	39.0
20	32.080992918325	34.0	30.0	36.0	23.0	39.0
21	32.63896381142019	35.0	31.0	37.0	23.0	39.0
22	33.07193440535709	35.0	31.0	38.0	25.0	39.0
23	33.9688779649578	35.0	32.0	38.0	27.0	40.0
24	32.42730094605817	35.0	31.0	37.0	22.0	39.0
25	31.56319300017471	35.0	30.0	37.0	18.0	39.0
26	31.013182110813016	33.0	27.0	37.0	20.0	39.0
27	32.550233463128144	35.0	30.0	38.0	22.0	39.0
28	32.40126220864974	35.0	31.0	38.0	21.0	39.0
29	32.607604843013696	35.0	31.0	38.0	21.0	40.0
30	31.736570784440897	35.0	30.0	38.0	18.0	40.0
31	31.908570047314875	35.0	30.0	37.0	20.0	40.0
32	31.832100402308065	35.0	30.0	38.0	18.0	40.0
33	31.83080803850287	35.0	30.0	38.0	16.0	40.0
34	31.232828912571588	35.0	30.0	38.0	12.0	40.0
35	30.777801976359317	35.0	29.0	38.0	10.0	40.0
36	30.287206316308435	35.0	27.0	38.0	9.0	40.0
37	30.525427257867264	35.0	28.0	38.0	8.0	40.0
38	30.337831078477596	35.0	26.0	38.0	8.0	40.0
39	29.98124397196049	35.0	25.0	38.0	8.0	40.0
40	29.559945337797572	34.0	24.0	38.0	8.0	40.0
41	28.923286241829988	33.0	23.0	37.0	8.0	40.0
42	29.609555355053022	34.0	24.0	38.0	8.0	40.0
43	29.468733172346287	34.0	23.0	38.0	7.0	40.0
44	29.67060518525078	35.0	24.0	38.0	7.0	40.0
45	29.965474740270775	35.0	25.0	38.0	7.0	40.0
46	29.6726346750782	35.0	24.0	38.0	7.0	40.0
47	29.66618482238374	34.0	24.0	38.0	7.0	40.0
48	29.524386187024188	34.0	24.0	38.0	7.0	40.0
49	29.398926380735162	34.0	24.0	37.0	7.0	40.0
50	29.56094332984714	34.0	24.0	38.0	7.0	40.0
51	27.791675741134743	32.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	23.0
11	29.0
12	38.0
13	55.0
14	97.0
15	195.0
16	415.0
17	788.0
18	1360.0
19	2204.0
20	3265.0
21	4751.0
22	6483.0
23	8805.0
24	11984.0
25	15943.0
26	20220.0
27	22470.0
28	23091.0
29	24773.0
30	27822.0
31	32026.0
32	35991.0
33	39684.0
34	42488.0
35	43024.0
36	30997.0
37	15582.0
38	3158.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.29759548534244	2.5622309071197282	33.57896223186443	36.56121137567341
2	35.97869035681207	15.37817197533021	34.060726739246455	14.582410928611258
3	13.31493709299515	15.339161734543689	57.46639255789909	13.87950861456207
4	11.821538918100034	3.0188661182895804	68.0058587159169	17.15373624769349
5	21.72296027895912	3.4807665153324603	56.987260643453574	17.80901256225484
6	14.454849834505634	13.71269795303933	59.21108369491599	12.621368517539052
7	53.360983536721086	1.5546657923267095	41.92619645365799	3.1581542172942205
8	53.54023918303461	10.657214860269146	31.21704771455034	4.585498242145898
9	49.26658354055031	3.085877574855387	31.385533662487227	16.262005222107078
10	29.096613767503754	19.37827727904767	37.197820213048566	14.327288740400009
11	24.953630465322767	16.32159755312453	44.327121211758595	14.397650769794106
12	19.860281113060292	14.956478452226815	46.30252322066633	18.88071721404656
13	17.79441363778872	15.897750090345802	53.17789866431808	13.129937607547404
14	15.566043380345059	19.17125974358545	46.25705116085383	19.005645715215667
15	12.22767621021494	15.782155327769786	54.533923353253286	17.456245108761987
16	14.414882287196743	16.073655163831045	45.12001033891045	24.39145221006177
17	15.00673704465117	17.152060961279343	49.77371667077511	18.06748532329438
18	15.386787734031529	15.306852639413746	48.36791204267673	20.938447583878002
19	15.558624254796705	18.96639614779855	44.68036731851263	20.794612278892107
20	17.713281910017972	18.03876612762332	50.53980121530063	13.708150747058076
21	18.260382587551664	22.18031347002075	44.21248375570495	15.346820186722637
22	15.74051249404675	15.600506415150333	48.07593355335428	20.583047537448635
23	16.389566316212704	22.408870402236268	45.37202128092399	15.829542000627036
24	17.184130729778698	16.805037346920702	45.024519013304165	20.986312909996435
25	14.689150605855367	23.59808442964874	42.73871036451839	18.974054599977503
26	15.278372770373277	17.298289532571157	47.06023133312113	20.36310636393443
27	16.047089907835314	18.852715998267275	44.08516198822991	21.015032105667494
28	12.599350467524573	19.71524917492144	48.292763480670786	19.392636876883202
29	17.14153058953329	18.09093933309241	43.54571976287517	21.221810314499127
30	14.76358118796953	20.328882655759756	45.70564260396947	19.201893552301243
31	17.7980035372476	16.878989275773684	41.419063323433186	23.90394386354553
32	18.604294955712607	20.90853175505398	41.722529491024055	18.764643798209356
33	15.821644221817493	18.01435481130292	40.90451106766003	25.259489899219556
34	17.87554536555946	22.756851323117278	39.401539827541235	19.966063483782033
35	14.888031035877455	22.010152235669718	39.51689526348666	23.584921464966172
36	16.889758974150332	25.832437852857197	34.9299610615572	22.347842111435266
37	16.51617010379596	21.45898300541596	37.0745669982936	24.95027989249448
38	17.923410691677898	22.321994835331314	37.452224421368044	22.302370051622756
39	18.58371286548168	22.421794040288244	34.487446121592285	24.507046972637784
40	18.390097621332618	20.424852634627214	41.777335289429665	19.40771445461051
41	17.082177585146432	26.06889256388226	33.68402662269438	23.16490322827692
42	17.542881349036353	21.366602926007385	40.743444245271505	20.347071479684757
43	18.915419575482424	23.93984285813435	34.38286038402351	22.76187718235971
44	20.337019761199887	20.544515949923294	35.77263012787222	23.345834161004596
45	17.776464140494305	19.953618498991236	36.50281567780891	25.76710168270554
46	21.18567199327971	23.616273253573745	33.99299730278887	21.20505745035767
47	15.649568374421726	20.52513049284533	43.61081660639624	20.214484526336697
48	19.13081354301537	22.566347325165914	34.78516845004894	23.517670681769772
49	17.302597411921816	19.490521468795396	40.97654838346828	22.23033273581451
50	18.688298603050455	20.542122683617375	37.42565916537231	23.34391954795986
51	18.439159580604013	19.712616581984925	36.082558114489075	25.765665722921987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64.0
1	403.0
2	742.0
3	16904.5
4	33067.0
5	22356.5
6	11646.0
7	10785.5
8	9925.0
9	9864.5
10	9804.0
11	9208.5
12	8613.0
13	8071.0
14	7529.0
15	6846.0
16	6163.0
17	5658.0
18	5153.0
19	4677.5
20	4202.0
21	4022.5
22	3843.0
23	3641.5
24	3440.0
25	3361.5
26	3452.5
27	3622.0
28	3845.0
29	4068.0
30	4552.0
31	5036.0
32	5622.5
33	6209.0
34	6938.0
35	7667.0
36	8421.0
37	9175.0
38	9523.5
39	9872.0
40	10729.5
41	11587.0
42	12532.5
43	13478.0
44	15218.5
45	16959.0
46	34895.0
47	52831.0
48	42340.5
49	31850.0
50	29875.0
51	27900.0
52	23998.5
53	20097.0
54	18920.5
55	17744.0
56	16927.0
57	16110.0
58	15248.5
59	14387.0
60	12939.5
61	11492.0
62	10209.0
63	8926.0
64	7743.5
65	6561.0
66	5758.5
67	4956.0
68	4206.5
69	3457.0
70	2841.0
71	2225.0
72	1909.5
73	1594.0
74	1371.0
75	931.0
76	714.0
77	528.0
78	342.0
79	259.5
80	177.0
81	141.0
82	105.0
83	77.0
84	49.0
85	32.5
86	16.0
87	10.5
88	5.0
89	3.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.660418299705015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22701834652258	35.02745179947285
2	6.809859337638503	5.946426144868984
3	3.1028985759456016	4.06421549302032
4	1.9571035362244928	3.4179183618957305
5	1.504686182328578	3.2847614065125965
6	1.2219543789741913	3.2010623597501677
7	0.9417418827588999	2.8781791172123734
8	0.7676923583224283	2.68142155918754
9	0.631513245432341	2.481491921164226
>10	2.7279427932229985	17.296813832103048
>50	0.06500190658860056	1.9755977791283161
>100	0.034742398349079606	3.0826665036553282
>500	0.0028018063184741615	0.8633912770952107
>1k	0.0028018063184741615	2.365824213519605
>5k	0.0011207225273896647	3.5754038320967436
>10k+	0.0011207225273896647	7.857374399317026
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21216	5.077553794643391	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	10900	2.6086602734546083	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	8477	2.0287718475297902	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	6137	1.468747531944122	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2357	0.564092868305735	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT	2286	0.5471006775336912	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	2024	0.4843971003185437	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC	1607	0.38459789536161054	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA	1396	0.3340999763066636	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	993	0.23765134417802072	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCACCCTCTATCGTATGCCG	915	0.21898386699183178	No Hit
GAATCTTTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	552	0.13210830008687557	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	535	0.12803974736680876	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCCTCTATCGT	534	0.12780042073621659	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCACCCTCTATCGTATGC	478	0.1143981294230553	No Hit
GAATGACTCTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	459	0.10985092344180412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16226345554148847	0.0
2	0.0	0.0	0.0	0.979563899013735	0.0
3	0.0	0.0	0.0	1.1949578665466842	0.0
4	0.0	0.0	0.0	2.052704510589007	0.0
5	0.0	0.0	0.0	4.022841333623716	0.0
6	0.0	0.0	0.0	4.373933500702424	0.0
7	0.0	0.0	0.0	4.847800229274912	0.0
8	0.0	0.0	0.0	5.53371035255206	0.0
9	0.0	0.0	0.0	5.708658119514933	0.0
10	0.0	0.0	0.0	9.09177936956579	0.0
11	0.0	0.0	0.0	9.65754752428567	0.0
12	0.0	0.0	0.0	12.482319745165004	0.0
13	0.0	0.0	0.0	12.764007189371982	0.0
14	0.0	0.0	0.0	12.941826875901961	0.0
15	0.0	0.0	0.0	13.509030990405396	0.0
16	0.0	0.0	0.0	13.829250022137714	0.0
17	0.0	0.0	0.0	14.124100431027262	0.0
18	0.0	0.0	0.0	14.389035011092789	0.0
19	0.0	0.0	0.0	15.302066106801902	0.0
20	0.0	0.0	0.0	15.589497390143093	0.0
21	0.0	0.0	0.0	15.888416351752708	0.0
22	0.0	0.0	0.0	16.32159755312453	0.0
23	0.0	0.0	0.0	16.620516514734145	0.0
24	0.0	0.0	0.0	16.859364492065126	0.0
25	0.0	0.0	0.0	17.082656238407615	0.0
26	0.0	0.0	0.0	17.281297341799114	0.0
27	0.0	0.0	0.0	17.49381938976496	0.0
28	0.0	0.0	0.0	17.67834022195152	0.0
29	0.0	0.0	0.0	17.890144290025585	0.0
30	0.0	0.0	0.0	18.136650719535513	0.0
31	0.0	0.0	0.0	18.336727782710565	0.0
32	0.0	0.0	0.0	18.55044646382937	0.0
33	0.0	0.0	0.0	18.757224672661	0.0
34	2.3932663059216588E-4	0.0	0.0	18.97788382606698	0.0
35	2.3932663059216588E-4	0.0	0.0	19.225826215360463	0.0
36	2.3932663059216588E-4	0.0	0.0	19.414415600267088	0.0
37	2.3932663059216588E-4	0.0	0.0	19.61090276398326	0.0
38	2.3932663059216588E-4	0.0	0.0	19.847118148377724	0.0
39	2.3932663059216588E-4	0.0	0.0	20.174277652397215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	40	6.7975634E-9	45.0	17
CGCGGGC	45	3.8380676E-10	45.0	4
ACGTCGG	20	7.028126E-4	45.0	43
CCTGATC	20	7.028126E-4	45.0	31
CTTACGG	20	7.028126E-4	45.0	2
CGTTGAT	30	2.1621727E-6	44.999996	25
CGTTTTT	10340	0.0	44.347195	1
ACGGCTG	1140	0.0	43.618423	8
CGGGATG	150	0.0	43.5	6
CGATGAA	145	0.0	43.448273	19
TACGGCT	1150	0.0	43.23913	7
TGATCCG	110	0.0	42.954544	4
GAATGAT	4160	0.0	41.80889	1
GATACCT	1365	0.0	41.538464	5
ACGGCGA	1470	0.0	41.47959	8
ATACGGC	2670	0.0	41.376404	6
ATGATAC	4000	0.0	41.343754	3
GGCGACT	245	0.0	41.32653	10
TGATACG	2685	0.0	41.312847	4
CGACCAC	840	0.0	41.25	12
>>END_MODULE