##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933406.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516258
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.423361962429638	31.0	28.0	31.0	25.0	34.0
2	28.606016759062328	31.0	27.0	33.0	16.0	34.0
3	28.59582611794878	31.0	27.0	33.0	16.0	34.0
4	33.124354489421954	35.0	32.0	37.0	28.0	37.0
5	34.27291780466356	35.0	33.0	37.0	30.0	37.0
6	33.584581740137686	35.0	33.0	37.0	28.0	37.0
7	33.65770990473755	35.0	33.0	37.0	29.0	37.0
8	33.51387290850699	35.0	33.0	37.0	28.0	37.0
9	34.70493822856014	37.0	34.0	39.0	28.0	39.0
10	34.6913674945473	37.0	33.0	39.0	27.0	39.0
11	34.87268962418016	37.0	34.0	39.0	27.0	39.0
12	34.969222752964605	37.0	34.0	39.0	27.0	39.0
13	34.97987634089932	37.0	34.0	39.0	27.0	39.0
14	35.66954507242503	38.0	34.0	40.0	27.0	41.0
15	36.01508548051556	38.0	34.0	40.0	27.0	41.0
16	36.069974702571194	38.0	34.0	40.0	29.0	41.0
17	35.3156658105056	37.0	33.0	40.0	27.0	41.0
18	35.19080382289476	37.0	33.0	39.0	27.0	40.0
19	34.55482530052803	37.0	33.0	38.0	27.0	40.0
20	33.79235188607247	35.0	32.0	38.0	27.0	40.0
21	34.175193411046415	35.0	33.0	38.0	27.0	40.0
22	34.78468130275947	35.0	33.0	39.0	29.0	40.0
23	35.12129400416071	35.0	34.0	39.0	30.0	40.0
24	34.6205773082451	35.0	33.0	39.0	27.0	40.0
25	33.489685389863205	35.0	32.0	38.0	24.0	40.0
26	33.60583467955944	35.0	32.0	38.0	24.0	40.0
27	34.36202054011754	35.0	33.0	38.0	27.0	40.0
28	34.10933680446598	35.0	33.0	39.0	25.0	40.0
29	34.100552436959816	35.0	33.0	39.0	25.0	40.0
30	33.39690426104777	35.0	33.0	39.0	22.0	40.0
31	33.2794223043517	35.0	33.0	39.0	22.0	40.0
32	32.73962049982761	35.0	32.0	39.0	18.0	40.0
33	32.195890426879586	35.0	31.0	39.0	15.0	40.0
34	31.59045864664567	35.0	30.0	39.0	10.0	40.0
35	31.234222036268687	35.0	29.0	39.0	8.0	41.0
36	30.54093689589314	35.0	25.0	39.0	7.0	40.0
37	30.46652448969314	35.0	25.0	39.0	7.0	40.0
38	30.205292702485966	35.0	24.0	39.0	7.0	40.0
39	29.86594493450949	35.0	23.0	39.0	7.0	40.0
40	29.625408226119497	35.0	22.0	39.0	7.0	40.0
41	29.094844050842795	35.0	21.0	39.0	7.0	40.0
42	29.256947107841427	35.0	21.0	39.0	7.0	40.0
43	29.174341124011637	35.0	21.0	39.0	7.0	40.0
44	29.31493942951005	35.0	21.0	39.0	7.0	40.0
45	29.405165634237147	35.0	20.0	39.0	7.0	40.0
46	29.219797078205083	35.0	20.0	39.0	7.0	40.0
47	29.052915402763734	35.0	20.0	39.0	7.0	40.0
48	28.943235746467852	35.0	20.0	38.0	7.0	40.0
49	29.099953899019482	35.0	20.0	39.0	7.0	40.0
50	28.998518182769082	35.0	20.0	39.0	7.0	40.0
51	27.81570648784135	33.0	19.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	21.0
10	32.0
11	29.0
12	47.0
13	57.0
14	81.0
15	136.0
16	295.0
17	539.0
18	1056.0
19	1922.0
20	3110.0
21	4475.0
22	6558.0
23	9493.0
24	13668.0
25	19761.0
26	25316.0
27	27232.0
28	25444.0
29	23984.0
30	24366.0
31	26735.0
32	31037.0
33	35995.0
34	39944.0
35	47351.0
36	50581.0
37	51434.0
38	39518.0
39	6022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.68042722824634	2.4516811361760977	31.3267784712295	37.54111316434806
2	37.77316767972603	17.33861751294895	31.46140108240453	13.426813724920486
3	12.590216519647154	17.480988188076505	56.65713654800507	13.271658744271274
4	11.169415292353824	2.8634907352525287	70.32200953786672	15.645084434526924
5	22.578439462439324	3.325662750020339	57.535767000220815	16.56013078731952
6	13.469815479856969	15.729344630010575	60.36884658445971	10.431993305672744
7	51.090152598119545	1.2414335467924953	44.92133778072204	2.747076074365918
8	50.21597728267649	11.96765958106218	34.37719124933657	3.4391718869247545
9	46.99317008162585	3.1135594993201074	33.838894506235256	16.054375912818784
10	29.093592738514463	19.048421525671273	39.68810168559131	12.16988405022295
11	25.69587299373569	15.871715305138128	45.79803121694967	12.634380484176516
12	19.201058385535912	14.939429510051175	48.01068458019053	17.848827524222386
13	19.05849400880955	15.605181905171445	54.200612871858645	11.135711214160361
14	15.135261826451115	21.19366673252521	47.917126707963845	15.753944733059827
15	11.742190920043855	15.531381596023694	57.43542957203569	15.290997911896763
16	14.831150316314712	17.884856021601603	45.9847595582054	21.29923410387829
17	15.4703655923976	18.120203464159392	51.69895672318879	14.710474220254213
18	15.818447365464555	16.163042509752877	50.001743314389316	18.016766810393253
19	14.933424760487974	19.464686261520402	46.90736027335169	18.69452870463993
20	16.31529196641989	19.601439590282375	52.448969313792716	11.634299129505015
21	16.357518914961126	23.687187414044917	47.75054333298467	12.20475033800929
22	14.034455640396857	15.298358572651658	51.74040886533477	18.92677692161671
23	14.188448411453189	22.806813647439846	49.71312793215795	13.291610008949014
24	15.588136164475902	18.794091326429808	46.95520456825851	18.662567940835785
25	13.915135455528048	26.370923065598983	44.344494419456936	15.369447059416027
26	14.184186976279303	17.25958726063325	49.36000991752186	19.19621584556559
27	16.469478439075036	19.34769049583735	46.388046286934056	17.79478477815356
28	11.196533516187642	20.637549442333096	50.893351773725534	17.27256526775372
29	17.420165886049226	20.707282017905776	44.96317732606565	16.90937476997935
30	17.535224635744143	21.521797240914424	47.6108844802405	13.332093643100931
31	18.003788803272784	18.449496182141488	45.80558557930337	17.74112943528236
32	19.783131689968968	24.91564295371694	41.3750489096537	13.92617644666039
33	18.335405940440634	18.432450441445944	39.457596783003844	23.77454683510958
34	20.3409922945504	25.30943830410376	40.083834051966264	14.265735349379574
35	19.266142122737083	26.26245017026371	36.79710532330734	17.674302383691874
36	20.76558619914849	25.499653274138122	37.11787517094166	16.61688535577173
37	20.475227502527808	27.767511593040688	36.18675158544759	15.57050931898392
38	19.723471597534566	23.823165936411637	35.84932340031535	20.60403906573845
39	23.284288088513883	26.02555311491541	33.57778475103533	17.112374045535372
40	19.92879529227634	25.518054926025357	35.06599413471559	19.48715564698271
41	25.116511511686017	24.036237695105935	32.758620689655174	18.088630103552873
42	17.94877754920989	26.10632668162043	38.77092461521177	17.173971153957904
43	22.75722603814372	22.679357995420894	33.810032968012116	20.75338299842327
44	23.391017669459842	22.42386558658655	34.53137772199172	19.65373902196189
45	18.275358444808603	22.10948789171306	35.51518814236292	24.09996552111541
46	22.563718140929534	25.499459572539312	32.626903602462335	19.30991868406882
47	15.942416388704872	22.795191551511067	42.33561513816735	18.92677692161671
48	20.752414490429206	25.46556179274704	32.74176865055844	21.040255066265317
49	18.423733869499358	22.61253094383041	38.790682178290695	20.173053008379533
50	19.88211320696241	21.63298195863309	35.78288375192249	22.702021082482016
51	18.7656171914043	22.316748602442964	34.180003021744945	24.737631184407796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	772.5
2	1474.0
3	17945.0
4	34416.0
5	25139.0
6	15862.0
7	14877.0
8	13892.0
9	13587.5
10	13283.0
11	12890.5
12	12498.0
13	11696.5
14	10895.0
15	10183.0
16	9471.0
17	8873.5
18	8276.0
19	7747.5
20	7219.0
21	6962.0
22	6705.0
23	6506.5
24	6308.0
25	6445.0
26	6820.5
27	7059.0
28	7383.0
29	7707.0
30	8286.5
31	8866.0
32	9435.5
33	10005.0
34	10727.0
35	11449.0
36	12027.5
37	12606.0
38	13302.0
39	13998.0
40	14614.5
41	15231.0
42	16096.0
43	16961.0
44	18641.5
45	20322.0
46	48450.0
47	76578.0
48	54442.5
49	32307.0
50	29702.0
51	27097.0
52	24196.5
53	21296.0
54	20748.5
55	20201.0
56	19286.5
57	18372.0
58	16544.0
59	14716.0
60	12695.5
61	10675.0
62	9106.0
63	7537.0
64	6529.0
65	5521.0
66	4574.5
67	3628.0
68	3050.5
69	2473.0
70	2045.0
71	1617.0
72	1359.5
73	1102.0
74	965.0
75	694.5
76	561.0
77	452.0
78	343.0
79	240.5
80	138.0
81	103.0
82	68.0
83	47.0
84	26.0
85	18.0
86	10.0
87	7.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	516258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.40711597264668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63851953131592	38.81422475670252
2	5.44349254810954	5.052335799527149
3	2.4417353029612006	3.3994168013707804
4	1.689164185411067	3.135569529968506
5	1.2926210846043749	2.9993408290961785
6	1.0548831554935076	2.937245096074722
7	0.8539517867964761	2.7740607723439044
8	0.7031807446523248	2.610607229344999
9	0.5524144079891876	2.307236354686367
>10	2.2183552608135892	15.351259648687634
>50	0.06785207806977794	2.1820730707223213
>100	0.037508291602816414	3.256583194396449
>500	0.001685765914733322	0.5225866491758642
>1k	0.0029500903507833133	3.579875009923285
>5k	0.0	0.0
>10k+	0.001685765914733322	11.077585257979395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17557	3.400818970359781	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCG	14924	2.8908026606851616	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC	13093	2.5361350332585646	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTC	11066	2.1435018924646205	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTACGAGT	3352	0.6492877592211647	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTC	2895	0.5607661285636252	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTA	2863	0.5545676774016093	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCT	2800	0.5423644766763905	No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	2639	0.511178519267498	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCT	2605	0.5045926649078561	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGT	1150	0.22275683863494608	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTAT	798	0.1545738758527713	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATG	718	0.13907774794773156	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTT	634	0.12280681364743985	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.10111223458038424	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11486504809610698	0.0
2	0.0	0.0	0.0	0.794757659929725	0.0
3	0.0	0.0	0.0	0.9072982888400761	0.0
4	0.0	0.0	0.0	1.6280619380232364	0.0
5	0.0	0.0	0.0	4.365065529250878	0.0
6	0.0	0.0	0.0	4.607192527767125	0.0
7	0.0	0.0	0.0	5.300644251517652	0.0
8	0.0	0.0	0.0	6.068283687613557	0.0
9	0.0	0.0	0.0	6.193996025243193	0.0
10	0.0	0.0	0.0	9.775732288894313	0.0
11	0.0	0.0	0.0	10.057374413568409	0.0
12	0.0	0.0	0.0	13.248801955611341	0.0
13	0.0	0.0	0.0	13.51436684758396	0.0
14	0.0	0.0	0.0	13.749714290141752	0.0
15	0.0	0.0	0.0	14.497983566356357	0.0
16	0.0	0.0	0.0	14.860011854537847	0.0
17	0.0	0.0	0.0	15.08393090276567	0.0
18	1.937015988129966E-4	0.0	0.0	15.238504778618443	0.0
19	1.937015988129966E-4	0.0	0.0	16.185705596813996	0.0
20	1.937015988129966E-4	0.0	0.0	16.420859337772974	0.0
21	1.937015988129966E-4	0.0	0.0	16.582406471183013	0.0
22	1.937015988129966E-4	0.0	0.0	16.883031352540783	0.0
23	1.937015988129966E-4	0.0	0.0	17.04302887316032	0.0
24	1.937015988129966E-4	0.0	0.0	17.185012145090244	0.0
25	1.937015988129966E-4	0.0	0.0	17.30220161237211	0.0
26	1.937015988129966E-4	0.0	0.0	17.45367626264387	0.0
27	1.937015988129966E-4	0.0	0.0	17.648346369450934	0.0
28	1.937015988129966E-4	0.0	0.0	17.782387875829528	0.0
29	1.937015988129966E-4	0.0	0.0	17.930957002119097	0.0
30	1.937015988129966E-4	0.0	0.0	18.09928369148759	0.0
31	1.937015988129966E-4	0.0	0.0	18.239329947429386	0.0
32	1.937015988129966E-4	0.0	0.0	18.3900297913059	0.0
33	1.937015988129966E-4	0.0	0.0	18.534724885619205	0.0
34	1.937015988129966E-4	0.0	0.0	18.756513216260085	0.0
35	1.937015988129966E-4	0.0	0.0	18.940336033533622	0.0
36	1.937015988129966E-4	0.0	0.0	19.107500513309237	0.0
37	1.937015988129966E-4	0.0	0.0	19.29190443537921	0.0
38	1.937015988129966E-4	0.0	0.0	19.503620282881815	0.0
39	1.937015988129966E-4	0.0	0.0	19.88463132774698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGC	20	7.029671E-4	45.000004	43
GTAGGCG	20	7.029671E-4	45.000004	19
AATGCGA	30	2.1631695E-6	45.000004	25
CGAGGCA	20	7.029671E-4	45.000004	32
AACGAGC	30	2.1631695E-6	45.000004	15
GATACGT	25	3.8878105E-5	45.0	5
CGATTAA	25	3.8878105E-5	45.0	19
TGCGAAT	25	3.8878105E-5	45.0	27
CGAATAT	25	3.8878105E-5	45.0	14
CGTTTTT	13315	0.0	44.44236	1
CACCGGT	140	0.0	43.392857	16
TACGGCT	1790	0.0	42.988827	7
GATACCT	1910	0.0	42.76178	5
ACGGCTG	1800	0.0	42.5	8
TGATACC	1930	0.0	42.318653	4
CGGCTGT	1835	0.0	42.179836	9
ATACCTG	1945	0.0	42.107967	6
ACCGGTC	75	0.0	42.0	17
TTGTTCG	65	0.0	41.538464	1
GCGCGAC	60	3.6379788E-12	41.250004	9
>>END_MODULE