##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933405.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348971
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.48309458379063	31.0	28.0	31.0	16.0	33.0
2	28.69013184476647	31.0	30.0	33.0	16.0	34.0
3	28.868487639374045	31.0	30.0	33.0	16.0	34.0
4	31.99474741454161	35.0	30.0	37.0	19.0	37.0
5	33.6629834570781	35.0	33.0	37.0	28.0	37.0
6	32.582988271231706	35.0	33.0	37.0	17.0	37.0
7	32.1991626811397	35.0	32.0	35.0	25.0	37.0
8	32.84522496138648	35.0	32.0	35.0	27.0	37.0
9	32.92450375532638	35.0	31.0	37.0	25.0	39.0
10	31.756151657301036	35.0	28.0	39.0	15.0	39.0
11	32.295846932839694	35.0	28.0	39.0	17.0	39.0
12	33.180525029300426	35.0	31.0	39.0	23.0	39.0
13	33.08605299580768	35.0	31.0	39.0	22.0	39.0
14	33.198182657011614	36.0	31.0	40.0	18.0	41.0
15	33.85737210255294	36.0	31.0	40.0	23.0	41.0
16	34.08652868003358	36.0	32.0	40.0	24.0	41.0
17	32.11121841069888	35.0	27.0	39.0	18.0	41.0
18	32.94421312945775	36.0	30.0	39.0	22.0	39.0
19	32.75971355786011	36.0	30.0	37.0	21.0	39.0
20	32.38884319900507	34.0	30.0	37.0	24.0	39.0
21	32.66049041324351	35.0	31.0	37.0	23.0	39.0
22	32.962100002579014	35.0	31.0	37.0	25.0	39.0
23	33.99991403297122	35.0	32.0	38.0	27.0	40.0
24	32.25148508042216	35.0	31.0	37.0	21.0	39.0
25	31.26078671293603	34.0	29.0	37.0	18.0	39.0
26	30.68650690171963	33.0	25.0	37.0	19.0	39.0
27	32.421198323069824	34.0	30.0	37.0	22.0	39.0
28	32.388304472291395	35.0	31.0	37.0	21.0	39.0
29	32.63300388857527	35.0	31.0	38.0	22.0	40.0
30	31.57723421143877	35.0	30.0	38.0	18.0	40.0
31	31.87290634465328	35.0	30.0	37.0	21.0	40.0
32	31.735723025695545	35.0	30.0	37.0	18.0	40.0
33	31.68587361127429	35.0	30.0	38.0	16.0	40.0
34	30.852947666138448	35.0	29.0	38.0	12.0	40.0
35	30.390232999303667	35.0	27.0	38.0	10.0	40.0
36	29.72886285679899	34.0	25.0	38.0	8.0	40.0
37	29.8316077840279	35.0	25.0	38.0	8.0	40.0
38	29.695395892495366	35.0	24.0	38.0	8.0	40.0
39	29.288293296577653	34.0	23.0	38.0	7.0	40.0
40	29.01778944382198	34.0	23.0	38.0	7.0	40.0
41	28.486450163480633	33.0	21.0	37.0	7.0	40.0
42	28.857566961151498	34.0	22.0	38.0	7.0	40.0
43	28.885921752810404	34.0	22.0	38.0	7.0	40.0
44	28.929805055434407	34.0	22.0	38.0	7.0	40.0
45	29.19794194933103	34.0	23.0	38.0	7.0	40.0
46	29.009579592573594	34.0	23.0	38.0	7.0	40.0
47	29.01184912213336	34.0	23.0	38.0	7.0	40.0
48	28.77034481375243	34.0	23.0	37.0	7.0	40.0
49	28.593378819443448	34.0	22.0	37.0	7.0	39.0
50	28.700144138051584	34.0	22.0	37.0	7.0	40.0
51	27.006413140346904	32.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	11.0
11	21.0
12	29.0
13	48.0
14	103.0
15	196.0
16	401.0
17	712.0
18	1238.0
19	1972.0
20	3037.0
21	4233.0
22	5747.0
23	7865.0
24	10743.0
25	14797.0
26	18102.0
27	19272.0
28	19334.0
29	20174.0
30	22467.0
31	25034.0
32	28535.0
33	31377.0
34	34966.0
35	37089.0
36	25532.0
37	13183.0
38	2690.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.193414352482012	2.4067902490464825	34.07733020795424	39.32246519051726
2	40.52543047989661	12.115333365809766	33.46323906571033	13.895997088583293
3	13.35669726137702	12.07149018113253	61.022262594886115	13.549549962604344
4	12.092695381564656	2.876456782941849	68.8693329818237	16.16151485366979
5	19.570107544753	3.29597588338286	60.512191557464654	16.621725014399477
6	13.821778887070845	11.38404050766396	62.6576420390233	12.136538566241894
7	51.376475409131416	1.2708792421146742	44.16527447839505	3.187370870358855
8	52.06793687727633	8.545695774147422	35.05850056308404	4.327866785492204
9	47.70281771264661	3.165019442876342	35.78893375094205	13.343229093534992
10	27.173604683483727	19.65894014115786	41.06243785300211	12.105017322356298
11	23.37013677354279	16.910860787859164	47.098182943568375	12.620819495029673
12	18.55770250250021	15.536534554447218	49.62819260053128	16.277570342521297
13	18.563147080989538	16.291038510363325	53.63740826601637	11.508406142630763
14	14.686893753349132	18.66114949379748	50.148866238168786	16.5030905146846
15	11.716732909038287	16.3707012903651	56.193494588375536	15.719071212221072
16	14.493754495359212	16.677317026343164	47.997111507833026	20.831816970464594
17	15.045089706594531	17.26762395729158	51.7825263417304	15.904759994383488
18	15.184069736453745	15.496130050921137	51.31715815927398	18.00264205335114
19	14.872009421986354	18.747689636101565	48.26131684294683	18.118984098965242
20	15.749159672293686	19.441443558347256	52.68403391685842	12.125362852500638
21	16.713136621667704	21.38200595464953	48.63842554252359	13.26643188115918
22	13.87679778548934	16.431737880798117	51.65644136618802	18.035022967524522
23	14.031251880528755	21.58947305076926	49.57116780477461	14.808107263927376
24	14.965426926592754	18.656564585595937	48.05155729272633	18.326451195084978
25	13.85874470944577	22.615919374389275	45.73474586713509	17.790590049029863
26	13.923793094555133	18.6436695312791	49.351092211100635	18.081445163065126
27	14.94078304500947	19.094423318843113	48.42092895971298	17.54386467643443
28	11.40897094600984	20.13319158325477	51.37962753351998	17.078209937215412
29	16.043453467480106	19.971287012387847	46.81277240802244	17.172487112109604
30	16.55065893727559	20.15812202160065	48.726685025403256	14.564534015720504
31	17.073625029013872	19.34687982669047	46.56747981923999	17.012015325055664
32	18.120703439540822	23.787936533408217	43.334546423628325	14.756813603422634
33	17.848187958311723	18.76144436070619	42.11696673935656	21.27340094162552
34	18.290345042997842	24.33124815529084	41.88657510222912	15.491831699482193
35	17.755916680755707	25.418444512581274	38.64705090107774	18.178587905585278
36	19.90967730842964	24.22006413140347	39.120729229649456	16.749529330517436
37	19.26607081963831	26.268658427204556	38.60664639755166	15.85862435560548
38	18.699548099985385	23.87906158391385	38.52612394726209	18.89526636883867
39	20.890847663559438	24.73787220141502	36.60705330815455	17.764226826871003
40	19.878156064544044	24.216625450252312	37.025139624782575	18.88007886042107
41	22.891013866481742	23.413120287932234	35.50237698834574	18.193488857240286
42	18.047344908316163	25.101226176387147	39.37948998627393	17.471938929022755
43	21.12410486831284	22.041659622146252	37.03459599794826	19.799639511592655
44	21.733611102355212	21.879755051279332	37.13145218370581	19.25518166265965
45	18.116118531339282	21.569987190912713	37.43892758997166	22.87496668777635
46	20.41315754031137	24.300300024930436	36.21848233807394	19.068060096684253
47	15.791856629920536	22.43194993280244	42.93078794513011	18.845405492146913
48	19.466660553455732	24.155875416581893	36.27149533915426	20.10596869080812
49	18.180020689398262	22.00211478890796	40.087858303412034	19.730006218281748
50	19.447747807124376	20.83525565161575	37.54294769479412	22.17404884646575
51	18.242490063644258	21.277126179539273	36.47896243527399	24.001421321542477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	324.5
2	564.0
3	16961.0
4	33358.0
5	22023.0
6	10688.0
7	9950.5
8	9213.0
9	9004.5
10	8796.0
11	8420.0
12	8044.0
13	7573.5
14	7103.0
15	6573.5
16	6044.0
17	5590.5
18	5137.0
19	4720.5
20	4304.0
21	4239.0
22	4174.0
23	4005.0
24	3836.0
25	3884.0
26	4038.0
27	4144.0
28	4353.5
29	4563.0
30	4972.5
31	5382.0
32	5901.0
33	6420.0
34	6778.0
35	7136.0
36	7527.0
37	7918.0
38	8446.0
39	8974.0
40	9667.5
41	10361.0
42	11563.5
43	12766.0
44	13606.5
45	14447.0
46	26110.0
47	37773.0
48	32292.5
49	26812.0
50	24952.5
51	23093.0
52	19660.5
53	16228.0
54	14985.5
55	13743.0
56	12553.5
57	11364.0
58	10233.0
59	9102.0
60	7986.0
61	6870.0
62	5955.0
63	5040.0
64	4359.0
65	3678.0
66	3154.5
67	2631.0
68	2227.5
69	1824.0
70	1509.5
71	1195.0
72	1002.0
73	809.0
74	693.0
75	487.5
76	398.0
77	329.0
78	260.0
79	180.0
80	100.0
81	70.5
82	41.0
83	35.0
84	29.0
85	19.5
86	10.0
87	6.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	348971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14657789443762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.29089755088735	39.13878531042546
2	7.108602136318797	6.845097329536773
3	3.2351094635621074	4.672783493532759
4	2.038907995269157	3.9266577045527247
5	1.4551259035184483	3.5029666329982447
6	1.1329282352971057	3.2727970517723914
7	0.8800988017171681	2.966162185817379
8	0.660164104207249	2.5427713973060753
9	0.4788191413469517	2.074815277758787
>10	1.6115623886014552	11.397624818818146
>50	0.06600273281400006	2.14691388051328
>100	0.032698601577578014	2.838159509342312
>500	0.003027648294220186	0.94226312626547
>1k	0.004238707611908261	3.259145881349533
>5k	0.0012110593176880744	3.5480638139389766
>10k+	6.055296588440372E-4	6.924992586071693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23753	6.806582781950363	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCG	6620	1.8970057683876311	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC	5550	1.5903900324095697	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTC	3180	0.9112505050562941	No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	1936	0.5547738923864733	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTACGAGT	1672	0.47912290706104516	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCT	1158	0.33183273108653727	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCC	1131	0.32409569849643666	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTA	1089	0.31206031446739124	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTC	1013	0.290282000510071	No Hit
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT	865	0.24787159964581584	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCT	745	0.21348478813425759	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGTACGAGTCGTATGCCG	544	0.15588687885239746	No Hit
TCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	540	0.1547406518020122	No Hit
CCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	538	0.15416753827681956	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC	452	0.12952365669353616	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGT	387	0.11089746712477543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8655676259631887E-4	0.0	0.0	0.3215166876330698	0.0
2	2.8655676259631887E-4	0.0	0.0	1.3840691633402202	0.0
3	2.8655676259631887E-4	0.0	0.0	1.671485596224328	0.0
4	2.8655676259631887E-4	0.0	0.0	2.4735579747314245	0.0
5	2.8655676259631887E-4	0.0	0.0	4.037584784982133	0.0
6	2.8655676259631887E-4	0.0	0.0	4.410395133119944	0.0
7	2.8655676259631887E-4	0.0	0.0	4.957145436153721	0.0
8	2.8655676259631887E-4	0.0	0.0	5.648033790773446	0.0
9	2.8655676259631887E-4	0.0	0.0	5.876992644087904	0.0
10	2.8655676259631887E-4	0.0	0.0	8.576070790982632	0.0
11	2.8655676259631887E-4	0.0	0.0	9.545778875608574	0.0
12	2.8655676259631887E-4	0.0	0.0	12.069770840556952	0.0
13	2.8655676259631887E-4	0.0	0.0	12.395585879628966	0.0
14	2.8655676259631887E-4	0.0	0.0	12.60706477042505	0.0
15	2.8655676259631887E-4	0.0	0.0	13.1947926905101	0.0
16	2.8655676259631887E-4	0.0	0.0	13.571901390086856	0.0
17	5.731135251926377E-4	0.0	0.0	13.983110344412573	0.0
18	5.731135251926377E-4	0.0	0.0	14.34990300053586	0.0
19	5.731135251926377E-4	0.0	0.0	15.260580392066963	0.0
20	8.596702877889567E-4	0.0	0.0	15.655742167687286	0.0
21	8.596702877889567E-4	0.0	0.0	16.038868559278566	0.0
22	8.596702877889567E-4	0.0	0.0	16.579601170297817	0.0
23	8.596702877889567E-4	0.0	0.0	16.929200420665328	0.0
24	0.0011462270503852755	0.0	0.0	17.196844436930288	0.0
25	0.0011462270503852755	0.0	0.0	17.444716036576104	0.0
26	0.0011462270503852755	0.0	0.0	17.675394230466143	0.0
27	0.0011462270503852755	0.0	0.0	17.945903814357067	0.0
28	0.0011462270503852755	0.0	0.0	18.176295451484506	0.0
29	0.0011462270503852755	0.0	0.0	18.452536170627358	0.0
30	0.0011462270503852755	0.0	0.0	18.729063446532805	0.0
31	0.0011462270503852755	0.0	0.0	18.9551567322213	0.0
32	0.0011462270503852755	0.0	0.0	19.19443162898923	0.0
33	0.0011462270503852755	0.0	0.0	19.438291433958696	0.0
34	0.0011462270503852755	0.0	0.0	19.71367248281376	0.0
35	0.0011462270503852755	0.0	0.0	19.99765023454671	0.0
36	0.0011462270503852755	0.0	0.0	20.23119399606271	0.0
37	0.0011462270503852755	0.0	0.0	20.458146952038994	0.0
38	0.0011462270503852755	2.8655676259631887E-4	0.0	20.740978476721562	0.0
39	0.0011462270503852755	2.8655676259631887E-4	0.0	21.226405632559725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	30	2.1611413E-6	45.000004	1
CGACCCT	30	2.1611413E-6	45.000004	12
TATACGG	25	3.8852028E-5	45.000004	27
GGTACCA	20	7.0265285E-4	45.0	8
ACCGCCT	35	1.2086821E-7	45.0	14
GACCGCC	45	3.8380676E-10	45.0	13
CTACGGG	20	7.0265285E-4	45.0	3
GTCGTTG	20	7.0265285E-4	45.0	1
AGCGACT	20	7.0265285E-4	45.0	19
GCGATCG	20	7.0265285E-4	45.0	9
CACCGAC	40	6.7921064E-9	45.0	16
CGTTTTT	9920	0.0	44.455643	1
CGATGAA	105	0.0	42.857147	19
TGTTCGG	90	0.0	42.5	2
ACCGGTG	80	0.0	42.1875	17
TGCTACG	80	0.0	42.1875	4
GCTACGG	80	0.0	42.1875	5
ACGGCTG	760	0.0	41.74342	8
GCGACCA	670	0.0	41.641792	11
CGACCAC	670	0.0	41.30597	12
>>END_MODULE