##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933404.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815599 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.27059743820186 31.0 28.0 31.0 25.0 34.0 2 28.308508225243042 31.0 26.0 33.0 16.0 34.0 3 28.311232603276856 31.0 25.0 33.0 16.0 34.0 4 32.94553818727095 35.0 32.0 37.0 28.0 37.0 5 34.17341242448801 35.0 33.0 37.0 30.0 37.0 6 33.42715476600633 35.0 32.0 37.0 28.0 37.0 7 33.56607474996904 35.0 32.0 37.0 29.0 37.0 8 33.33582189286647 35.0 32.0 37.0 28.0 37.0 9 34.64165846206285 37.0 34.0 39.0 28.0 39.0 10 34.53876843890196 37.0 32.0 39.0 27.0 39.0 11 34.80671383854075 37.0 34.0 39.0 27.0 39.0 12 34.90088021196691 37.0 34.0 39.0 27.0 39.0 13 35.00064860305125 37.0 34.0 39.0 27.0 39.0 14 35.786526221832055 38.0 34.0 40.0 27.0 41.0 15 36.02151792731477 38.0 34.0 40.0 27.0 41.0 16 36.0085066313225 38.0 34.0 40.0 29.0 41.0 17 35.259680308583015 37.0 33.0 40.0 27.0 41.0 18 35.06266805133405 37.0 33.0 39.0 27.0 40.0 19 34.45318961891812 37.0 33.0 38.0 27.0 40.0 20 33.64004247185198 35.0 32.0 38.0 25.0 40.0 21 34.165651257542 35.0 33.0 38.0 27.0 40.0 22 34.73880546690224 35.0 33.0 39.0 29.0 40.0 23 35.01010177795706 35.0 33.0 39.0 29.0 40.0 24 34.57058799728788 35.0 33.0 39.0 27.0 40.0 25 33.57266132008499 35.0 33.0 38.0 24.0 40.0 26 33.58883716139917 35.0 32.0 38.0 24.0 40.0 27 34.32560608828603 35.0 33.0 38.0 27.0 40.0 28 33.980863144756185 35.0 33.0 39.0 24.0 40.0 29 34.10566344490368 35.0 33.0 39.0 24.0 40.0 30 33.38096417479668 35.0 33.0 39.0 22.0 40.0 31 33.343353780473 35.0 33.0 39.0 21.0 40.0 32 32.756300583987965 35.0 32.0 39.0 18.0 40.0 33 32.113894205363174 35.0 31.0 39.0 14.0 40.0 34 31.528425120678175 35.0 30.0 39.0 10.0 40.0 35 31.09567814575545 35.0 28.0 40.0 8.0 41.0 36 30.612736160784895 35.0 25.0 40.0 7.0 40.0 37 30.527044540270403 35.0 24.0 40.0 7.0 40.0 38 30.279601863170505 35.0 23.0 39.0 7.0 40.0 39 29.92148592629466 35.0 23.0 39.0 7.0 40.0 40 29.761036980182663 35.0 22.0 39.0 7.0 40.0 41 29.19145437892886 35.0 21.0 39.0 7.0 40.0 42 29.47004348950894 35.0 21.0 39.0 7.0 40.0 43 29.430151336624984 35.0 21.0 39.0 7.0 40.0 44 29.563054883588627 35.0 21.0 39.0 7.0 40.0 45 29.676187685369893 35.0 22.0 39.0 7.0 40.0 46 29.4936788789589 35.0 22.0 39.0 7.0 40.0 47 29.478199458312233 35.0 22.0 39.0 7.0 40.0 48 29.163821927197066 35.0 20.0 38.0 7.0 40.0 49 29.415358527904033 35.0 22.0 39.0 7.0 40.0 50 29.431421568687554 35.0 22.0 39.0 7.0 40.0 51 28.05399221921557 33.0 20.0 38.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 16.0 9 35.0 10 41.0 11 48.0 12 85.0 13 93.0 14 162.0 15 259.0 16 380.0 17 833.0 18 1766.0 19 3155.0 20 5039.0 21 7594.0 22 10776.0 23 15623.0 24 22357.0 25 32154.0 26 40310.0 27 41260.0 28 37863.0 29 34995.0 30 36772.0 31 41701.0 32 48702.0 33 57168.0 34 65093.0 35 75374.0 36 82659.0 37 82842.0 38 61550.0 39 8888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.75861912532997 2.4513271840696222 30.718036682242133 37.07201700835828 2 36.46644981173346 19.165791032112594 30.886379213314385 13.481379942839558 3 12.020245243066752 19.479425551036723 55.1581107872864 13.342218418610125 4 10.896040823983355 2.7081936098499386 70.75707547458984 15.638690091576866 5 23.402186613764854 3.203780289088143 56.44857337981042 16.945459717336583 6 12.804944586739317 16.830084391962227 59.98033347269921 10.38463754859925 7 50.809405112071005 1.2411736649995893 45.26010944103659 2.689311781892817 8 49.321296372359456 12.784591447512812 34.21583400666259 3.6782781734651464 9 46.51195011273923 2.7309989345254224 33.10756879299754 17.64948215973781 10 29.88551972231452 16.210539738278246 40.493551365315554 13.41038917409168 11 25.692650432381598 15.45980316307401 44.771388881055515 14.076157523488872 12 18.31880617803602 13.910757614955388 48.40037812699623 19.37005808001236 13 18.159904560942326 14.791950456045189 55.293594033342366 11.75455094967012 14 15.665541522243162 21.2708696307867 46.95653133463871 16.107057512331426 15 12.039862726658566 14.816227091990058 57.24835366399419 15.895556517357182 16 14.34320051888244 17.051026300915034 45.67281225209938 22.93296092810315 17 15.02306893461125 17.977339354266007 50.91190646383823 16.08768524728451 18 15.431357811865881 16.05838163116924 49.13762768223109 19.37263287473378 19 15.094182312631574 19.1231230053004 45.78561278275231 19.99708189931572 20 17.139795414167992 18.669836525057043 51.805728059990265 12.3846400007847 21 16.86159497498158 23.362583818763877 46.54775202029428 13.228069185960257 22 14.686629091011639 14.387462466236473 50.77446146942308 20.151446973328806 23 15.27196575768239 22.287300499387566 48.73326230169483 13.707471441235214 24 17.09038387737111 17.642983868298025 45.906628134659314 19.360004119671554 25 14.124097749016368 26.553490134244896 43.59090680591811 15.731505310820637 26 14.57738422925972 15.542932249794323 49.582699341220376 20.296984179725573 27 17.70257197470816 18.386976933517573 45.34752985229261 18.562921239481657 28 12.87213446804128 19.19509464822787 50.4147258640582 17.518045019672655 29 17.151075467233284 16.10485054542735 49.33024684924822 17.413827138091147 30 15.45465357363116 20.58670989052218 50.61715377287123 13.341482762975431 31 21.746348389343293 15.572481084454493 44.09899963094609 18.582170895256127 32 19.366257192566444 19.840877686215897 46.09838903676929 14.694476084448363 33 17.83854565785392 16.774297172998004 42.51832089053566 22.868836278612406 34 22.59725674013823 19.269028039514517 42.62315181847943 15.510563401867831 35 15.575668925538164 20.89789222399733 41.156009264356626 22.37042958610788 36 21.638084401770968 20.813046607462734 37.03180116699505 20.51706782377124 37 17.500511893712474 21.519030798223145 43.6812698397129 17.29918746835148 38 22.477835308773063 21.70711342215966 36.08623845786961 19.72881281119766 39 23.854369610556166 20.724645322027126 37.24048214870298 18.18050291871373 40 19.231509602145174 20.841614567943314 39.210322719866014 20.7165531100455 41 22.406844540025183 23.09137210810705 32.3302260056719 22.171557346195865 42 18.317580085311533 21.545759619616994 38.99097473145504 21.145685563616436 43 20.3575531603153 20.198283715404262 34.91507468743831 24.529088436842127 44 21.89642213882067 20.84087891230862 35.809999767042385 21.452699181828326 45 19.35902324549196 20.06844049588094 34.61137151958254 25.961164739044555 46 23.397895289229144 22.96790457075107 33.71203250617032 19.922167633849476 47 15.902177418069419 20.977833469633975 43.958244186174824 19.16174492612178 48 20.969005602017656 24.017072115095775 32.59775943815527 22.416162844731296 49 18.38550562224819 20.105836323977837 39.75998008825415 21.748677965519818 50 19.468268107243876 19.838793328584266 36.62130532283634 24.071633241335512 51 18.760322168124286 20.480285042036588 34.865785759913884 25.89360702992525 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 77.0 1 1136.0 2 2195.0 3 25095.0 4 47995.0 5 35671.5 6 23348.0 7 22310.5 8 21273.0 9 20742.5 10 20212.0 11 19507.0 12 18802.0 13 17795.5 14 16789.0 15 15668.0 16 14547.0 17 13491.5 18 12436.0 19 11778.5 20 11121.0 21 10412.0 22 9703.0 23 9376.5 24 9050.0 25 8981.0 26 8998.0 27 9084.0 28 9538.0 29 9992.0 30 10972.5 31 11953.0 32 13077.0 33 14201.0 34 15483.0 35 16765.0 36 18115.0 37 19465.0 38 20489.0 39 21513.0 40 22632.0 41 23751.0 42 25421.5 43 27092.0 44 30452.5 45 33813.0 46 85004.5 47 136196.0 48 94321.0 49 52446.0 50 47668.0 51 42890.0 52 37932.5 53 32975.0 54 32177.0 55 31379.0 56 29850.0 57 28321.0 58 26268.5 59 24216.0 60 21619.0 61 19022.0 62 16531.0 63 14040.0 64 12054.0 65 10068.0 66 8474.0 67 6880.0 68 5649.5 69 4419.0 70 3701.5 71 2984.0 72 2514.5 73 2045.0 74 1816.0 75 1309.0 76 1031.0 77 790.0 78 549.0 79 396.5 80 244.0 81 186.5 82 129.0 83 90.5 84 52.0 85 34.5 86 17.0 87 13.5 88 10.0 89 7.0 90 4.0 91 4.5 92 5.0 93 2.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 815599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.29256078698592 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20251786453606 35.18847544416174 2 5.5993745247614335 4.736237749151466 3 2.3467905088116514 2.977553407447152 4 1.5219409001665904 2.574671121379824 5 1.1080079862236152 2.343024755491405 6 0.9683562202672951 2.4572558585466306 7 0.7624905339037282 2.257337407823734 8 0.6758055111433291 2.2865236528167467 9 0.5849745708315398 2.2266065336160508 >10 3.1051100777415153 20.330107934586394 >50 0.07071280694547734 2.0976745125461456 >100 0.04802576301011533 3.786629107604289 >500 0.0017678194973040865 0.4542011023171732 >1k 0.002062456080188101 2.3902566651983586 >5k 8.839097486520432E-4 2.048702420630081 >10k+ 0.0011785463315360577 11.844742326682827 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG 25678 3.148360897941268 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC 23650 2.899709293415024 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC 22944 2.813147147066144 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22783 2.793407054201881 No Hit GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT 5858 0.7182451180052942 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT 5352 0.6562048261461821 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC 5231 0.6413691041798727 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA 4800 0.5885245077544234 No Hit GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC 4393 0.5386225338677463 TruSeq Adapter, Index 21 (95% over 21bp) GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT 4280 0.5247676860810275 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGT 1875 0.22989238584157165 No Hit GAATGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT 1536 0.18832784248141549 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTAT 1258 0.15424246474063846 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATG 1040 0.12751364334679174 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.452185448976764E-4 0.0 0.0 0.08190299399582393 0.0 2 2.452185448976764E-4 0.0 0.0 0.7061068000328593 0.0 3 2.452185448976764E-4 0.0 0.0 0.7999028934562206 0.0 4 2.452185448976764E-4 0.0 0.0 1.6662600125797113 0.0 5 2.452185448976764E-4 0.0 0.0 5.083993482091077 0.0 6 2.452185448976764E-4 0.0 0.0 5.37604876906421 0.0 7 2.452185448976764E-4 0.0 0.0 6.035318827021612 0.0 8 2.452185448976764E-4 0.0 0.0 6.850793098078835 0.0 9 2.452185448976764E-4 0.0 0.0 6.968375390357271 0.0 10 2.452185448976764E-4 0.0 0.0 10.652538808899962 0.0 11 2.452185448976764E-4 0.0 0.0 10.850184956087489 0.0 12 2.452185448976764E-4 0.0 0.0 14.249772253276426 0.0 13 2.452185448976764E-4 0.0 0.0 14.501979526703687 0.0 14 2.452185448976764E-4 0.0 0.0 14.728316243644242 0.0 15 2.452185448976764E-4 0.0 0.0 15.49683116335356 0.0 16 2.452185448976764E-4 0.0 0.0 15.847371073284789 0.0 17 2.452185448976764E-4 0.0 0.0 16.01706230635398 0.0 18 2.452185448976764E-4 0.0 0.0 16.14800900932934 0.0 19 2.452185448976764E-4 0.0 0.0 17.07567076467725 0.0 20 2.452185448976764E-4 0.0 0.0 17.27797606421783 0.0 21 2.452185448976764E-4 0.0 0.0 17.41762802553706 0.0 22 2.452185448976764E-4 0.0 0.0 17.72157641193773 0.0 23 2.452185448976764E-4 0.0 0.0 17.87226320777735 0.0 24 2.452185448976764E-4 0.0 0.0 17.999409023306796 0.0 25 2.452185448976764E-4 0.0 0.0 18.10448516979545 0.0 26 2.452185448976764E-4 0.0 0.0 18.25333282654834 0.0 27 2.452185448976764E-4 0.0 0.0 18.44877200683179 0.0 28 2.452185448976764E-4 0.0 0.0 18.568193438196957 0.0 29 2.452185448976764E-4 0.0 0.0 18.705761041884553 0.0 30 2.452185448976764E-4 0.0 0.0 18.87263226168742 0.0 31 2.452185448976764E-4 0.0 0.0 19.01314248791379 0.0 32 2.452185448976764E-4 0.0 0.0 19.173024979187076 0.0 33 2.452185448976764E-4 0.0 0.0 19.325550914113432 0.0 34 2.452185448976764E-4 0.0 0.0 19.557159829769287 0.0 35 2.452185448976764E-4 0.0 0.0 19.74364853316397 0.0 36 2.452185448976764E-4 0.0 0.0 19.91027453442194 0.0 37 2.452185448976764E-4 0.0 0.0 20.08744493311051 0.0 38 2.452185448976764E-4 0.0 0.0 20.292938073734764 0.0 39 2.452185448976764E-4 0.0 0.0 20.644336248573136 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACGA 40 6.8102963E-9 45.0 22 TTACGCC 20 7.0320815E-4 45.0 39 TATCGAA 20 7.0320815E-4 45.0 10 CGTTTTT 20410 0.0 44.327534 1 CCGATGA 910 0.0 43.763737 18 CGATGAA 905 0.0 43.25967 19 GATACCT 3265 0.0 42.588055 5 TGATACC 3295 0.0 42.26859 4 ATACCTG 3300 0.0 42.204544 6 CTACGGC 80 0.0 42.1875 6 GAATCTG 2875 0.0 41.713043 1 TACGGCT 2900 0.0 41.66379 7 ACGGCTG 2930 0.0 41.31399 8 GGCGACT 770 0.0 41.2013 10 CGACCAC 2660 0.0 40.93985 12 CGGCTGT 2935 0.0 40.783646 9 ACCACCG 1625 0.0 40.56923 14 AATCTGT 2965 0.0 40.522762 2 GCGACCA 2700 0.0 40.5 11 TACCTGT 3490 0.0 40.293697 7 >>END_MODULE