##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933403.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523010
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.21020056977878	31.0	26.0	31.0	16.0	33.0
2	28.321035926655323	31.0	28.0	33.0	16.0	34.0
3	28.491451406282863	31.0	28.0	33.0	16.0	34.0
4	31.72845261084874	35.0	28.0	37.0	19.0	37.0
5	33.57233131297681	35.0	33.0	37.0	28.0	37.0
6	32.29608038087226	35.0	32.0	37.0	17.0	37.0
7	32.105575419208044	35.0	32.0	35.0	25.0	37.0
8	32.59969599051643	35.0	32.0	35.0	25.0	37.0
9	32.879948758149936	35.0	31.0	37.0	25.0	39.0
10	31.588306150934017	35.0	28.0	39.0	15.0	39.0
11	32.21877210760788	35.0	27.0	39.0	17.0	39.0
12	33.07701573583679	35.0	31.0	39.0	23.0	39.0
13	33.19644939867307	35.0	31.0	39.0	23.0	39.0
14	33.47029884705837	36.0	31.0	40.0	20.0	41.0
15	33.91053708342097	36.0	32.0	40.0	23.0	41.0
16	34.121022542590005	36.0	32.0	40.0	25.0	41.0
17	32.12913519817977	35.0	27.0	39.0	18.0	41.0
18	32.834268943232445	36.0	30.0	39.0	22.0	39.0
19	32.646176937343455	36.0	30.0	37.0	20.0	39.0
20	32.12971071298828	34.0	30.0	36.0	23.0	39.0
21	32.610898453184454	35.0	31.0	37.0	23.0	39.0
22	32.9497906349783	35.0	31.0	37.0	25.0	39.0
23	33.89100017208084	35.0	32.0	38.0	27.0	40.0
24	32.28394294564158	35.0	31.0	37.0	21.0	39.0
25	31.464232041452362	34.0	29.0	37.0	18.0	39.0
26	30.750478958337318	33.0	25.0	37.0	19.0	39.0
27	32.463392669356224	34.0	30.0	37.0	22.0	39.0
28	32.38174604692071	35.0	31.0	37.0	21.0	39.0
29	32.64206038125466	35.0	31.0	38.0	22.0	40.0
30	31.650972256744613	35.0	30.0	38.0	18.0	40.0
31	31.947218982428634	35.0	30.0	37.0	20.0	40.0
32	31.769964245425516	35.0	30.0	38.0	18.0	40.0
33	31.58506529511864	35.0	30.0	38.0	15.0	40.0
34	30.737192405498938	35.0	29.0	38.0	10.0	40.0
35	30.251482763235884	35.0	27.0	38.0	8.0	40.0
36	29.657423376226077	35.0	24.0	38.0	7.0	40.0
37	29.725555916712874	35.0	24.0	38.0	7.0	40.0
38	29.675920154490353	35.0	24.0	38.0	8.0	40.0
39	29.210915661268427	34.0	23.0	38.0	7.0	40.0
40	29.035071987151298	34.0	23.0	38.0	7.0	40.0
41	28.347945545974266	33.0	21.0	38.0	7.0	40.0
42	28.92895164528403	34.0	22.0	38.0	7.0	40.0
43	28.951649108047647	34.0	22.0	38.0	7.0	40.0
44	28.974061681421006	34.0	22.0	38.0	7.0	40.0
45	29.256163362077206	35.0	23.0	38.0	7.0	40.0
46	28.980055830672455	34.0	22.0	38.0	7.0	40.0
47	29.111630752758074	34.0	23.0	38.0	7.0	40.0
48	28.74847517255884	34.0	22.0	38.0	7.0	40.0
49	28.695566050362327	34.0	22.0	37.0	7.0	40.0
50	28.905066824726106	34.0	22.0	38.0	7.0	40.0
51	27.128431578746103	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	26.0
11	32.0
12	45.0
13	56.0
14	118.0
15	251.0
16	566.0
17	1079.0
18	1879.0
19	2906.0
20	4795.0
21	6488.0
22	8815.0
23	12284.0
24	16556.0
25	22447.0
26	27796.0
27	29071.0
28	28394.0
29	29706.0
30	33184.0
31	37866.0
32	43011.0
33	47893.0
34	52017.0
35	53410.0
36	38572.0
37	19753.0
38	3912.0
39	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.59778971721382	2.4569319898281106	33.38349171143955	38.56178658151852
2	39.3485784210627	14.607942486759335	32.53704518078048	13.506433911397487
3	12.609127932544311	14.631269000592722	59.322192692300334	13.437410374562628
4	11.552933978317814	2.768398309783752	69.56387067168887	16.114797040209556
5	20.78067340968624	3.298598497160666	59.28108449169231	16.639643601460776
6	13.246591843368197	12.911416607713043	62.21104758991224	11.63094395900652
7	51.13076231812011	1.3166096250549704	44.608133687692394	2.944494369132521
8	51.421005334506035	9.84474484235483	34.58939599625246	4.144853826886676
9	47.6094147339439	2.7043459972084665	34.45268732911417	15.233551939733466
10	28.245540238236362	16.95206592608172	41.25064530314908	13.551748532532839
11	23.829181086403704	16.024741400738034	46.56889925622837	13.577178256629892
12	18.3476415364907	14.050018164088641	49.66769277834076	17.934647521079903
13	17.636182864572376	14.947515343874878	55.436798531576834	11.979503259975909
14	15.357832546222824	18.388558536165654	49.38796581327317	16.86564310433835
15	12.123095160704384	14.952868970000575	56.84575820730006	16.078277661994992
16	14.151737060476854	15.427238484923805	47.622033995525896	22.79899045907344
17	15.041777403873729	16.549014359190075	51.8496778264278	16.5595304105084
18	15.287279401923481	14.708322976616126	50.69998661593469	19.304411005525708
19	15.213093439895985	18.30729813961492	47.25588420871494	19.223724211774154
20	16.48190283168582	17.999847039253552	52.592493451368036	12.925756677692588
21	17.136192424619033	21.17798894858607	47.48513412745454	14.200684499340358
22	14.525152482744117	14.781744134911378	51.24127645742911	19.451826924915395
23	14.926674442171278	21.07779965966234	49.15450947400623	14.84101642416015
24	16.0195789755454	16.91688495439858	47.76983231678171	19.293703753274315
25	14.223055008508442	22.65119213781763	45.63297068889696	17.492782164776965
26	14.186153228427756	16.078468862928048	50.51222729966922	19.22315060897497
27	15.726850347029695	17.634270855241773	47.91342421751018	18.72545458021835
28	13.024607560084894	18.245731439169425	51.598248599453164	17.13141240129252
29	16.428940173228046	15.77388577656259	50.34894170283552	17.448232347373853
30	15.226286304277165	19.2055601231334	51.46306953977935	14.105084032810081
31	19.617598133878893	15.744058431005142	46.65474847517256	17.983594959943407
32	18.118965220550276	19.172673562646985	47.30521404944456	15.403147167358178
33	16.83180053918663	16.467180359840157	44.786715359170955	21.91430374180226
34	20.398462744498193	18.998680713561882	44.49647234278503	16.106384199154892
35	14.793216190895011	20.557924322670694	43.18024511959619	21.46861436683811
36	20.603047742872985	19.503642377774806	39.799047819353355	20.09426205999885
37	17.400432114108717	20.762700521978548	44.57639433280434	17.260473031108393
38	21.176841742987705	20.712797078449743	39.425823598019164	18.68453758054339
39	22.13131680082599	19.901149117607694	39.89885470641097	18.068679375155348
40	18.93921722338005	19.874763388845338	40.771878166765454	20.414141221009157
41	21.076078851264793	21.359821035926654	35.594348100418735	21.96975201238982
42	17.68761591556567	20.509550486606372	40.4212156555324	21.38161794229556
43	19.06655704479838	19.365786505038145	38.008451081241276	23.5592053689222
44	20.811647960842052	20.04818263513126	38.54324009101164	20.596929313015046
45	18.857574424963193	19.314162253111792	37.4717500621403	24.356513259784705
46	21.306475975602762	21.743370107646125	37.20158314372574	19.748570773025374
47	15.945393013517906	19.7581308196784	45.16548440756391	19.130991759239784
48	20.217969063689033	22.154643314659374	36.276744230511845	21.350643391139748
49	17.863903175847497	18.984531844515402	41.08028527179213	22.071279707844973
50	18.789506892793636	18.684919982409514	38.774593220014914	23.750979904781936
51	18.48913022695551	19.216649777250915	37.27844591881608	25.015774076977497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	426.0
2	764.0
3	22349.0
4	43934.0
5	29790.5
6	15647.0
7	14841.0
8	14035.0
9	13584.5
10	13134.0
11	12518.5
12	11903.0
13	11253.5
14	10604.0
15	9769.0
16	8934.0
17	8334.5
18	7735.0
19	7142.5
20	6550.0
21	6026.0
22	5502.0
23	5318.5
24	5135.0
25	5090.0
26	5061.5
27	5078.0
28	5417.0
29	5756.0
30	6336.5
31	6917.0
32	7647.5
33	8378.0
34	9278.0
35	10178.0
36	10985.5
37	11793.0
38	12458.0
39	13123.0
40	14082.5
41	15042.0
42	16435.5
43	17829.0
44	20166.5
45	22504.0
46	43473.5
47	64443.0
48	52538.5
49	40634.0
50	37289.0
51	33944.0
52	28909.0
53	23874.0
54	22164.0
55	20454.0
56	18752.0
57	17050.0
58	15850.0
59	14650.0
60	13480.5
61	12311.0
62	10734.5
63	9158.0
64	7848.0
65	6538.0
66	5670.0
67	4802.0
68	3975.5
69	3149.0
70	2644.5
71	2140.0
72	1831.5
73	1523.0
74	1353.5
75	1001.0
76	818.0
77	613.0
78	408.0
79	288.0
80	168.0
81	135.0
82	102.0
83	67.5
84	33.0
85	21.0
86	9.0
87	7.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	523010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.454650453342815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90700862918618	38.04961455547778
2	6.832772896373207	6.348281530561843
3	2.866464398250494	3.9948180497303505
4	1.8370982297038034	3.413670244373803
5	1.315644808424883	3.0558909848066564
6	1.0280833281070418	2.865555098647319
7	0.7899264483747216	2.5687029930168768
8	0.6512815403227447	2.4204045041926237
9	0.5089983057102776	2.1280804535803206
>10	2.151412746688519	14.672177672622619
>50	0.06737103641560939	2.223640672012214
>100	0.03640546688093781	3.3417787211289873
>500	0.00292917549616741	0.8141102486235936
>1k	0.00292917549616741	2.588124128503946
>5k	8.3690728461926E-4	3.375019612369348
>10k+	8.3690728461926E-4	8.140130530351716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29336	5.609070572264393	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	12539	2.3974684996462785	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	9711	1.8567522609510334	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	7651	1.4628783388462936	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT	2844	0.5437754536242136	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	2400	0.45888223934532796	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	2387	0.45639662721554086	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC	1912	0.36557618401177794	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA	1670	0.3193055582111241	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	1094	0.20917382076824534	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTGTTGCCTCGTATGCCG	1007	0.19253933959197722	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGT	733	0.14015028393338558	No Hit
GAATCTTTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	660	0.1261926158199652	No Hit
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	638	0.12198619529263303	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT	560	0.10707252251390986	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTTGTTGCCTCGTATGC	552	0.10554291504942545	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14856312498804994	0.0
2	0.0	0.0	0.0	0.8261792317546509	0.0
3	0.0	0.0	0.0	1.0175713657482648	0.0
4	0.0	0.0	0.0	1.8003479856981701	0.0
5	0.0	0.0	0.0	3.774115217682262	0.0
6	0.0	0.0	0.0	4.141794611957707	0.0
7	0.0	0.0	0.0	4.618649739010726	0.0
8	0.0	0.0	0.0	5.31787155121317	0.0
9	0.0	0.0	0.0	5.520162138391235	0.0
10	0.0	0.0	0.0	8.626794898759107	0.0
11	0.0	0.0	0.0	9.272480449704595	0.0
12	0.0	0.0	0.0	11.963059979732702	0.0
13	0.0	0.0	0.0	12.26324544463777	0.0
14	0.0	0.0	0.0	12.468404045811743	0.0
15	0.0	0.0	0.0	13.042006844993404	0.0
16	0.0	0.0	0.0	13.398405384218275	0.0
17	0.0	0.0	0.0	13.732815816141184	0.0
18	0.0	0.0	0.0	14.042561327699278	0.0
19	0.0	0.0	0.0	14.960325806389935	0.0
20	0.0	0.0	0.0	15.30410508403281	0.0
21	0.0	0.0	0.0	15.664901244718074	0.0
22	0.0	0.0	0.0	16.170627712663237	0.0
23	0.0	0.0	0.0	16.523202233226897	0.0
24	0.0	0.0	0.0	16.788015525515764	0.0
25	0.0	0.0	0.0	17.023383874113307	0.0
26	0.0	0.0	0.0	17.249192176057818	0.0
27	0.0	0.0	0.0	17.496988585304297	0.0
28	0.0	0.0	0.0	17.72929771897287	0.0
29	0.0	0.0	0.0	17.979579740349134	0.0
30	0.0	0.0	0.0	18.273264373530143	0.0
31	0.0	0.0	0.0	18.509971128659107	0.0
32	0.0	0.0	0.0	18.758532341637828	0.0
33	0.0	0.0	0.0	18.998298311695763	0.0
34	0.0	0.0	0.0	19.27114204317317	0.0
35	0.0	0.0	0.0	19.566547484751727	0.0
36	0.0	0.0	0.0	19.800577426817842	0.0
37	0.0	0.0	0.0	20.05238905565859	0.0
38	0.0	0.0	0.0	20.365958585877898	0.0
39	0.0	0.0	0.0	20.862889810902278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGT	25	3.8878796E-5	45.0	4
ACCGCCG	25	3.8878796E-5	45.0	14
TCGTCGG	20	7.029758E-4	45.0	2
AACGAAT	25	3.8878796E-5	45.0	24
GTGCGAA	25	3.8878796E-5	45.0	43
CGTTTTT	14560	0.0	44.62912	1
AATGATC	390	0.0	43.26923	2
ATCCGGC	115	0.0	43.043476	6
GATACCT	1585	0.0	42.72871	5
GCGACCG	90	0.0	42.500004	11
TACGGCT	1425	0.0	42.31579	7
TGATACC	1590	0.0	42.31132	4
TGATCCG	160	0.0	42.1875	4
ACGGCTG	1430	0.0	42.01049	8
GAATGAT	5200	0.0	41.971153	1
CGGGATA	60	3.6379788E-12	41.249996	6
AATGATA	4970	0.0	41.106636	2
ATGATAC	5010	0.0	41.0479	3
ATGATCC	210	0.0	40.714287	3
CGGGAAT	105	0.0	40.714287	6
>>END_MODULE