##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933402.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580823
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.080678623263886	31.0	28.0	31.0	25.0	34.0
2	27.896465876867826	31.0	25.0	33.0	16.0	34.0
3	27.843959347339894	31.0	25.0	33.0	16.0	34.0
4	32.83383750299144	35.0	32.0	37.0	28.0	37.0
5	34.16880529868824	35.0	33.0	37.0	30.0	37.0
6	33.22410786074243	35.0	32.0	37.0	28.0	37.0
7	33.76656743965029	35.0	33.0	37.0	30.0	37.0
8	33.224035549556405	35.0	32.0	37.0	28.0	37.0
9	34.77183926945042	37.0	34.0	39.0	28.0	39.0
10	34.833811677567866	37.0	33.0	39.0	28.0	39.0
11	35.176907594912734	37.0	34.0	39.0	30.0	39.0
12	35.319257329685634	37.0	34.0	39.0	30.0	39.0
13	35.341007501424706	37.0	34.0	39.0	30.0	39.0
14	36.11863855253666	38.0	34.0	40.0	29.0	41.0
15	36.288871136301424	38.0	34.0	40.0	29.0	41.0
16	36.37763139545094	38.0	34.0	40.0	30.0	41.0
17	35.508951952660276	37.0	33.0	40.0	27.0	41.0
18	35.2944511494896	37.0	33.0	39.0	27.0	40.0
19	34.57720854718219	37.0	33.0	37.0	27.0	40.0
20	33.34599525156545	35.0	32.0	37.0	25.0	39.0
21	33.96548173884299	35.0	33.0	37.0	27.0	39.0
22	34.454014389926016	35.0	33.0	38.0	28.0	40.0
23	34.62072438591447	35.0	33.0	38.0	29.0	40.0
24	34.20547912186673	35.0	33.0	38.0	27.0	40.0
25	33.214004955037936	35.0	32.0	38.0	24.0	40.0
26	33.14926578320762	35.0	31.0	38.0	24.0	40.0
27	33.93010779531802	35.0	33.0	38.0	25.0	40.0
28	33.41185524677914	35.0	33.0	38.0	24.0	40.0
29	33.36284892299375	35.0	33.0	38.0	23.0	40.0
30	32.64337672578393	35.0	31.0	38.0	21.0	40.0
31	32.694077197356165	35.0	32.0	38.0	21.0	40.0
32	31.8818331918674	35.0	31.0	38.0	15.0	40.0
33	30.583050257995982	35.0	25.0	38.0	10.0	40.0
34	30.068885013162358	35.0	23.0	39.0	8.0	40.0
35	29.50026428016797	35.0	21.0	39.0	7.0	40.0
36	28.827703104043746	35.0	18.0	39.0	7.0	40.0
37	28.70533708203704	35.0	18.0	39.0	7.0	40.0
38	28.18498062232384	35.0	18.0	38.0	7.0	40.0
39	28.10592383566078	34.0	17.0	38.0	7.0	40.0
40	27.667867835812288	33.0	15.0	38.0	7.0	40.0
41	27.290868302391605	33.0	15.0	38.0	7.0	40.0
42	27.70978938506223	34.0	15.0	38.0	7.0	40.0
43	27.6023952219523	34.0	15.0	38.0	7.0	40.0
44	27.682631369625515	34.0	15.0	39.0	7.0	40.0
45	27.748357072636587	34.0	15.0	39.0	7.0	40.0
46	27.514721352288046	34.0	15.0	38.0	7.0	40.0
47	27.35106047797694	33.0	15.0	38.0	7.0	40.0
48	27.25773084054867	34.0	15.0	38.0	7.0	40.0
49	27.47154296575721	34.0	15.0	38.0	7.0	40.0
50	27.381257284921567	34.0	13.0	38.0	7.0	40.0
51	26.509962587569706	32.0	12.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	17.0
10	26.0
11	34.0
12	49.0
13	79.0
14	123.0
15	188.0
16	357.0
17	714.0
18	1593.0
19	2830.0
20	4512.0
21	6761.0
22	9671.0
23	13633.0
24	19606.0
25	28277.0
26	35908.0
27	36360.0
28	31866.0
29	28265.0
30	27781.0
31	30373.0
32	33880.0
33	38646.0
34	44174.0
35	49639.0
36	50657.0
37	47162.0
38	32999.0
39	4636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.68469568181701	2.2185760550115954	24.51194253671084	41.58478572646055
2	41.23373213526324	23.1022187482245	25.061335381002475	10.602713735509784
3	9.705538520340964	23.233411900010847	56.27669703162581	10.784352548022374
4	8.406347544776981	2.4219082233313762	76.81978847256393	12.351955759327712
5	24.53673494334763	2.7183840860296513	58.22204010516112	14.522840865461594
6	10.48253943111757	19.374404939198346	61.57504093329638	8.568014696387712
7	40.97857695029295	1.106877654638332	55.31478608801649	2.5997593070522345
8	38.84849601341544	15.684296248598969	41.55327870969297	3.9139290282926127
9	36.553476704607085	2.307932020598358	40.14785915846996	20.990732116324594
10	26.773216625374687	13.949860800966905	46.521057189539675	12.755865384118742
11	23.149909697102217	13.195930601921757	51.13141180703932	12.522747893936707
12	15.605098282953673	12.061677998288635	53.236183828808436	19.09703988994926
13	15.485440487033056	14.161112765851215	61.00653727555555	9.346909471560183
14	13.620844904557844	21.78288394226813	49.711185679630454	14.885085473543574
15	9.028223744583117	13.703658429504342	63.101495636364255	14.166622189548278
16	10.156450416047575	16.287405973936984	49.969612084920875	23.586531525094564
17	11.003352139980683	17.72347169447491	56.77495553722907	14.49822062831534
18	11.235264443728985	15.54897102903983	54.3370355512781	18.878728975953088
19	11.138677359539825	18.637691689206523	50.75591014818628	19.46772080306737
20	14.200195240202266	16.76465980169518	58.409704849842385	10.625440108260175
21	14.010636631125145	23.97615108217133	51.0334473669259	10.979764919777626
22	11.463216849195021	13.423538668406726	55.536884730804395	19.57635975159386
23	13.079371856830738	22.464330785798772	52.67818250999014	11.778114847380355
24	14.426253781272436	16.74675417467972	50.215642286892916	18.611349757154933
25	10.254070517179931	27.686231433672564	48.20814602727509	13.851552021872413
26	11.827183152182334	14.922101914008227	54.22615839937468	19.02455653443476
27	16.465601396638906	18.649571384053317	47.96986345237706	16.91496376693072
28	9.635293368203394	18.89749545042121	54.91879625978999	16.548414921585405
29	16.73917871709626	13.621533582519977	49.09171985269178	20.547567847691983
30	15.13507557379787	22.701236004772536	50.27245821876889	11.891230202660708
31	16.503650854046757	19.570678158406263	45.11236641799653	18.81330456955045
32	21.416162927432282	21.440610995088004	43.699371409190064	13.443854668289651
33	14.243926290797711	21.782367433796527	41.74800240348609	22.225703871919674
34	17.460568882430618	23.587220203056695	39.94332180371645	19.008889110796233
35	18.855830433712164	20.166212426160808	39.13205916432373	21.8458979758033
36	14.523185204442662	25.316662735463304	39.41321194236454	20.746940117729498
37	22.853261664913408	21.034979675391643	40.131503056869306	15.980255602825647
38	16.610223768686847	29.091306645914507	35.35500488100506	18.94346470439359
39	22.334170650955627	25.12228338065125	36.396802468221814	16.146743500171308
40	17.02188102055187	24.699779450882627	37.66190388466021	20.616435643905287
41	16.549447938528605	28.489402107010225	38.392074694011775	16.5690752604494
42	17.055454071205858	21.355559266764573	40.754584443109174	20.834402218920395
43	19.21222127911601	21.043071641446705	37.724057070742724	22.02065000869456
44	20.72851798224244	22.48275292128583	35.834324742649656	20.954404353822078
45	15.78243285820293	21.958668992102588	36.16402931702085	26.09486883267364
46	24.23165060612269	23.831184371142328	33.616093026619126	18.321071996115855
47	14.303496934522222	21.539091943673032	45.485285534491574	18.672125587313175
48	19.10547619498539	24.834760331460703	33.408973129507615	22.650790344046293
49	16.204248110009416	19.77504334366924	41.58943430270495	22.431274243616386
50	18.481533961292858	19.6340365309225	38.907377979177824	22.97705152860682
51	17.723299524984377	20.14520774831575	34.97072257813482	27.160770148565057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	788.0
2	1537.0
3	19885.5
4	38234.0
5	29563.5
6	20893.0
7	20256.5
8	19620.0
9	19372.5
10	19125.0
11	18363.0
12	17601.0
13	16865.5
14	16130.0
15	15024.0
16	13918.0
17	13081.0
18	12244.0
19	11438.0
20	10632.0
21	9948.5
22	9265.0
23	8672.5
24	8080.0
25	7777.5
26	7323.5
27	7172.0
28	7241.0
29	7310.0
30	7587.5
31	7865.0
32	8523.5
33	9182.0
34	10346.5
35	11511.0
36	12207.5
37	12904.0
38	14032.0
39	15160.0
40	15632.0
41	16104.0
42	16405.5
43	16707.0
44	18717.0
45	20727.0
46	65092.5
47	109458.0
48	73152.5
49	36847.0
50	32316.0
51	27785.0
52	23683.5
53	19582.0
54	17864.5
55	16147.0
56	14948.0
57	13749.0
58	12111.0
59	10473.0
60	9374.0
61	8275.0
62	7210.5
63	6146.0
64	5254.0
65	4362.0
66	3645.0
67	2928.0
68	2403.5
69	1879.0
70	1607.5
71	1336.0
72	1162.5
73	989.0
74	775.5
75	482.0
76	402.0
77	339.5
78	277.0
79	198.5
80	120.0
81	82.5
82	45.0
83	27.0
84	9.0
85	7.5
86	6.0
87	5.5
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	580823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.42957097933563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.54488032985638	39.316967838433186
2	5.337369292816335	4.849487942618523
3	1.9355046119372272	2.6378743244650114
4	1.1577623717868728	2.1038659138518283
5	0.8065715539015216	1.8321099828941103
6	0.6027585275021334	1.6429836785134802
7	0.48364621407067127	1.538028800770736
8	0.4074313405973041	1.480754480549688
9	0.3288567243149945	1.3445837909369869
>10	2.2775195172835123	17.038445307334964
>50	0.0668747249680157	2.120066049585881
>100	0.04279982397058222	3.597966661535654
>500	0.0026749889981613887	0.6734143633339832
>1k	0.003439271569064643	4.523976513049824
>5k	3.82141285451627E-4	0.9157671478697502
>10k+	0.001528565141806508	14.383707204256382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	22166	3.8163089271602537	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	20294	3.49400764088199	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	20204	3.4785123867339967	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20189	3.4759298443759974	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	5275	0.9081940625629494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	4749	0.8176329105424543	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTC	4603	0.7924961649245985	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	4185	0.7205293178816955	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	3737	0.6433973861227947	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	3626	0.6242865726736029	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	1662	0.28614569326627903	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	1414	0.24344765961403042	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1064	0.18318833792738923	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATG	1019	0.1754407108533925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	602	0.10364603330102287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0995139655282246	0.0
2	1.7216949053326056E-4	0.0	0.0	0.8425974866697772	0.0
3	1.7216949053326056E-4	0.0	0.0	0.948826062328799	0.0
4	1.7216949053326056E-4	0.0	0.0	2.0381424289327383	0.0
5	1.7216949053326056E-4	0.0	0.0	6.0774108463335645	0.0
6	1.7216949053326056E-4	0.0	0.0	6.424504539248618	0.0
7	1.7216949053326056E-4	0.0	0.0	7.236800195584541	0.0
8	1.7216949053326056E-4	0.0	0.0	8.216961105190393	0.0
9	1.7216949053326056E-4	0.0	0.0	8.347982087486205	0.0
10	1.7216949053326056E-4	0.0	0.0	12.91684385776734	0.0
11	1.7216949053326056E-4	0.0	0.0	13.146690127629244	0.0
12	1.7216949053326056E-4	0.0	0.0	17.483123085690476	0.0
13	1.7216949053326056E-4	0.0	0.0	17.8160988803818	0.0
14	1.7216949053326056E-4	0.0	0.0	18.086577150009557	0.0
15	1.7216949053326056E-4	0.0	0.0	19.03368151743302	0.0
16	1.7216949053326056E-4	0.0	0.0	19.489414158874563	0.0
17	1.7216949053326056E-4	0.0	0.0	19.676045886612616	0.0
18	1.7216949053326056E-4	0.0	0.0	19.833236631469482	0.0
19	1.7216949053326056E-4	0.0	0.0	20.961291133443407	0.0
20	1.7216949053326056E-4	0.0	0.0	21.189243538909444	0.0
21	1.7216949053326056E-4	0.0	0.0	21.350910690520177	0.0
22	1.7216949053326056E-4	0.0	0.0	21.67699970559017	0.0
23	1.7216949053326056E-4	0.0	0.0	21.835395636880772	0.0
24	1.7216949053326056E-4	0.0	0.0	21.994996754605104	0.0
25	1.7216949053326056E-4	0.0	0.0	22.10793994039492	0.0
26	1.7216949053326056E-4	0.0	0.0	22.291816956284446	0.0
27	1.7216949053326056E-4	0.0	0.0	22.494976955113692	0.0
28	1.7216949053326056E-4	0.0	0.0	22.618078140844972	0.0
29	1.7216949053326056E-4	0.0	0.0	22.763905699326646	0.0
30	1.7216949053326056E-4	0.0	0.0	22.908528071374583	0.0
31	1.7216949053326056E-4	0.0	0.0	23.055388646799454	0.0
32	1.7216949053326056E-4	0.0	0.0	23.205864781525523	0.0
33	1.7216949053326056E-4	0.0	0.0	23.34824895019653	0.0
34	1.7216949053326056E-4	0.0	0.0	23.558467898137643	0.0
35	1.7216949053326056E-4	0.0	0.0	23.732186914085702	0.0
36	1.7216949053326056E-4	0.0	0.0	23.889721997923637	0.0
37	1.7216949053326056E-4	0.0	0.0	24.070844301964627	0.0
38	1.7216949053326056E-4	0.0	0.0	24.24576850434642	0.0
39	1.7216949053326056E-4	0.0	0.0	24.460636028531926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCCG	20	7.0304004E-4	45.000004	37
GGAACGT	20	7.0304004E-4	45.000004	8
CGTTAGG	35	1.2105374E-7	45.0	2
TATCGGG	35	1.2105374E-7	45.0	3
CGATACG	25	3.8884144E-5	45.0	4
CGTTTTT	18290	0.0	44.483326	1
ACGGCTG	2430	0.0	43.24074	8
CGGCTGT	2505	0.0	43.203594	9
TACGGCT	2445	0.0	42.97546	7
GATACCT	2605	0.0	42.58157	5
TGATACC	2615	0.0	42.50478	4
TGATGCG	85	0.0	42.35294	4
CCGATGA	645	0.0	42.2093	18
CACCGGT	225	0.0	42.0	16
CGACCCT	65	0.0	41.538464	12
TACCTGT	2700	0.0	41.333336	7
ATACCTG	2685	0.0	41.312847	6
GTATGAT	170	0.0	41.02941	1
CCGGTGA	105	0.0	40.714287	18
GATGAAT	725	0.0	40.655174	20
>>END_MODULE