SRR2933401.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933401.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363958
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23644	6.4963539749091925	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCG	10503	2.8857725341935057	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGC	8153	2.240093637177916	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	5998	1.6479923507657475	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGAACTT	2331	0.6404585144439743	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	1998	0.548964440951978	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGC	1764	0.48467130822787247	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTA	1455	0.3997714021947587	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTC	1438	0.3951005335780502	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	926	0.25442496112188767	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGAGAACTTCGTATGCCG	850	0.23354343083542606	No Hit
GAATCTTTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	596	0.1637551585622517	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGT	568	0.1560619631935553	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	487	0.13380664801982645	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	468	0.12858626544821106	No Hit
GAATGACTCTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	462	0.1269377235834904	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCCGAGAACTTCGTATGC	435	0.11951928519224744	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	398	0.10935327702647009	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCGAGAACTTCGTATGCCGTCTTC	391	0.10742997818429599	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGCACTTCGTATGCCGTCTTCTGC	380	0.10440765143230812	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCCGAGAACTTCGTATGCCGTCTTC	364	0.10001153979305305	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGA	25	3.8855345E-5	45.000004	4
TGGGTGC	50	2.1827873E-11	45.000004	6
GATCCGT	50	2.1827873E-11	45.000004	5
CCGTGTC	30	2.1613996E-6	45.000004	19
TCGGGCA	50	2.1827873E-11	45.000004	5
TCACGGG	20	7.026928E-4	45.0	3
TCACGAT	20	7.026928E-4	45.0	22
TGTCACG	20	7.026928E-4	45.0	20
TCGTTAG	20	7.026928E-4	45.0	1
GGGTCCA	20	7.026928E-4	45.0	7
GGATATC	20	7.026928E-4	45.0	8
GCTTGCG	20	7.026928E-4	45.0	1
ACGGGAG	35	1.208864E-7	45.0	5
CGTTAGG	20	7.026928E-4	45.0	2
GTCACGA	20	7.026928E-4	45.0	21
GCTAGTA	20	7.026928E-4	45.0	12
GATTCTA	20	7.026928E-4	45.0	44
TCTATTG	20	7.026928E-4	45.0	1
TAGTAAG	20	7.026928E-4	45.0	31
CGTTTTT	12820	0.0	44.526134	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content