##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933400.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 480897 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.329654790942758 31.0 28.0 31.0 25.0 34.0 2 28.274258313110707 31.0 25.0 33.0 16.0 34.0 3 28.227778505584357 31.0 25.0 33.0 16.0 34.0 4 33.04032464332279 35.0 32.0 37.0 28.0 37.0 5 34.23546622249671 35.0 33.0 37.0 30.0 37.0 6 33.437045770715976 35.0 32.0 37.0 28.0 37.0 7 33.7328929063812 35.0 33.0 37.0 29.0 37.0 8 33.36723248429497 35.0 32.0 37.0 28.0 37.0 9 34.80419299766894 37.0 34.0 39.0 28.0 39.0 10 34.82387080809404 37.0 33.0 39.0 28.0 39.0 11 35.1485557198319 37.0 34.0 39.0 28.0 39.0 12 35.25271107950351 37.0 34.0 39.0 30.0 39.0 13 35.28982505609309 37.0 34.0 39.0 30.0 39.0 14 36.21044007344608 38.0 34.0 40.0 30.0 41.0 15 36.32255763708237 38.0 34.0 40.0 29.0 41.0 16 36.38405729293383 38.0 34.0 41.0 30.0 41.0 17 35.53255478823948 38.0 33.0 40.0 27.0 41.0 18 35.27142818524549 37.0 33.0 39.0 27.0 40.0 19 34.54587572806651 37.0 33.0 37.0 27.0 40.0 20 33.48416604803108 35.0 32.0 37.0 25.0 39.0 21 34.08457736272008 35.0 33.0 38.0 27.0 40.0 22 34.48725194792232 35.0 33.0 38.0 28.0 40.0 23 34.71122714427414 35.0 33.0 39.0 29.0 40.0 24 34.28118703173445 35.0 33.0 38.0 27.0 40.0 25 33.45457343256456 35.0 33.0 38.0 24.0 40.0 26 33.29520666587648 35.0 32.0 38.0 24.0 40.0 27 34.00507801046794 35.0 33.0 38.0 25.0 40.0 28 33.58460751470689 35.0 33.0 38.0 24.0 40.0 29 33.60584075176181 35.0 33.0 39.0 23.0 40.0 30 32.96239319438466 35.0 32.0 38.0 21.0 40.0 31 32.866466207940576 35.0 32.0 38.0 21.0 40.0 32 32.14601047625583 35.0 31.0 38.0 16.0 40.0 33 31.358394832989184 35.0 30.0 39.0 12.0 40.0 34 30.603422354475075 35.0 25.0 39.0 8.0 40.0 35 30.016042936429216 35.0 23.0 39.0 7.0 40.0 36 29.43496008500781 35.0 21.0 39.0 7.0 40.0 37 29.286186023202475 35.0 20.0 39.0 7.0 40.0 38 28.948564869400307 35.0 18.0 39.0 7.0 40.0 39 28.462627132213342 35.0 18.0 38.0 7.0 40.0 40 28.251334485347176 34.0 18.0 38.0 7.0 40.0 41 27.59213511417206 33.0 16.0 38.0 7.0 40.0 42 27.783531608639688 34.0 15.0 38.0 7.0 40.0 43 27.763862116004052 34.0 15.0 38.0 7.0 40.0 44 27.906447742447966 34.0 15.0 38.0 7.0 40.0 45 27.96551860377586 34.0 16.0 38.0 7.0 40.0 46 27.73260178374995 34.0 15.0 38.0 7.0 40.0 47 27.555027375924574 33.0 15.0 38.0 7.0 40.0 48 27.416868892923016 34.0 15.0 38.0 7.0 40.0 49 27.60616930444565 34.0 15.0 38.0 7.0 40.0 50 27.615402050750994 34.0 15.0 38.0 7.0 40.0 51 26.254326810106946 31.0 14.0 37.0 7.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 17.0 9 27.0 10 32.0 11 26.0 12 36.0 13 59.0 14 89.0 15 113.0 16 241.0 17 489.0 18 1023.0 19 2116.0 20 3317.0 21 5031.0 22 7163.0 23 10100.0 24 14676.0 25 21190.0 26 27381.0 27 30065.0 28 27929.0 29 24822.0 30 24502.0 31 25780.0 32 28441.0 33 32266.0 34 36288.0 35 40930.0 36 41822.0 37 40431.0 38 30128.0 39 4365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.576597483452797 2.0673865713447994 25.43496840279727 43.92104754240513 2 41.00628616938762 20.775134800175504 27.118904879839135 11.099674150597737 3 9.897129738800617 20.786155871215666 57.85459256348033 11.462121826503388 4 9.017107613480642 2.3343876131479298 75.33629862527734 13.312206148094083 5 23.28066093155083 2.6567019548884687 58.61359085209514 15.449046261465552 6 11.260207487258187 17.77906703514474 62.02076536139756 8.939960116199519 7 43.71497430842779 1.0478335277616622 52.69860281931474 2.5385893444958065 8 41.70934732385521 13.995512552584024 40.689794280272075 3.6053458432886876 9 39.17574865303797 2.3876214657192705 39.390347621216186 19.046282260026576 10 27.03593492993302 14.467755049418066 45.424487988072286 13.071822032576621 11 22.596522748114463 12.413261051742888 51.563016612705006 13.427199587437643 12 16.46485629978977 10.924376737638205 53.8718270232503 18.738939939321725 13 16.066018294977926 12.070984015288097 61.438104209425305 10.424893480308674 14 13.366063834875245 19.760988319744143 51.161267381580664 15.71168046379994 15 9.574191562850256 12.417212001738417 62.967953636641525 15.040642798769799 16 11.404105244990092 14.968901864640452 50.68029120580915 22.94670168456031 17 12.085332202113966 16.016735392402115 56.898670609298875 14.999261796185046 18 12.0558040495158 14.461308762583256 54.78948714589611 18.69340004200484 19 11.817499381364408 17.57632091695311 51.115727484263786 19.4904522174187 20 14.979091156734187 15.822099118938151 57.819865792467 11.378943931860668 21 14.423046931047606 22.345533451030054 51.25484251305373 11.97657710486861 22 12.583983680497072 12.588974354175633 55.41436107939954 19.412680885927756 23 14.151471105039123 20.3132895401718 53.09681698991676 12.438422364872311 24 15.117582351314315 15.773232105835552 50.09638238541725 19.01280315743288 25 11.41387864761061 25.580321773685426 48.60271534237061 14.403084236333353 26 12.493319775336506 13.394552263790374 55.611284744966184 18.50084321590694 27 16.101992734410903 16.474629702410287 48.77468563954444 18.648691923634374 28 9.869473088831912 17.86869121662227 55.9941110050593 16.26772468948652 29 15.874085303089853 13.258348461312922 53.21305809767997 17.654508137917265 30 12.041039973216717 20.282929608627214 50.77053922149649 16.905491196659575 31 16.118212423866233 17.147746814806496 46.644291813007776 20.089748948319492 32 17.515601053863925 18.814215933973387 45.95807418220534 17.71210882995735 33 14.85078925424779 16.53493367602626 42.20113662593029 26.41314044379566 34 20.214307845546966 18.321802797688484 46.212182650338846 15.251706706425699 35 14.113209273503474 19.886586940654652 43.1587221380046 22.84148164783727 36 15.98325628980842 26.99455392734827 40.19717319925057 16.825016583592745 37 15.747239013759703 20.12385188512301 40.90376941424047 23.225139686876815 38 16.613328841727025 24.71132071940561 36.23707363531068 22.438276803556686 39 22.39460840886926 20.98079214467963 37.06282218437628 19.56177726207483 40 16.381262515673832 20.27835482442186 42.64073179911706 20.699650860787237 41 19.50459245950796 27.857732529003094 34.7604580606658 17.87721695082315 42 16.333019336781057 21.32306918113442 44.773828907229614 17.570082574854908 43 18.422655994942787 23.604638831184225 36.30403184049807 21.668673333374922 44 20.53849368991697 21.069376602474126 36.66315240061801 21.728977306990892 45 16.46922313925851 21.424962102071753 38.403649846016926 23.702164912652812 46 22.186663672262462 23.882660944027513 35.04950124454925 18.881174139160777 47 14.433860057351158 20.171055340332753 45.39163271968841 20.003451882627672 48 19.999500932632145 22.572193213931467 34.84862662898708 22.57967922444931 49 16.39311536566042 19.334077775490385 43.173694159040295 21.0991126998089 50 17.593580330091473 19.663254293538948 39.5527524605061 23.190412915863483 51 18.164804521550355 19.09140626787025 37.62947159162981 25.11431761894959 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 819.0 2 1591.0 3 19713.5 4 37836.0 5 28533.5 6 19231.0 7 18501.5 8 17772.0 9 17080.5 10 16389.0 11 15436.0 12 14483.0 13 13437.0 14 12391.0 15 11335.0 16 10279.0 17 9441.0 18 8603.0 19 7873.0 20 7143.0 21 6590.5 22 6038.0 23 5727.0 24 5416.0 25 5155.0 26 4973.5 27 5053.0 28 5279.0 29 5505.0 30 5814.0 31 6123.0 32 6550.0 33 6977.0 34 7741.5 35 8506.0 36 8753.0 37 9000.0 38 9473.5 39 9947.0 40 10491.0 41 11035.0 42 11723.0 43 12411.0 44 14744.0 45 17077.0 46 49119.0 47 81161.0 48 54959.0 49 28757.0 50 25886.0 51 23015.0 52 20033.0 53 17051.0 54 16188.0 55 15325.0 56 14671.0 57 14017.0 58 13119.5 59 12222.0 60 11208.0 61 10194.0 62 9109.0 63 8024.0 64 7083.5 65 6143.0 66 5048.5 67 3954.0 68 3389.0 69 2824.0 70 2212.0 71 1600.0 72 1399.5 73 1199.0 74 992.5 75 606.5 76 427.0 77 322.5 78 218.0 79 173.0 80 128.0 81 102.5 82 77.0 83 46.0 84 15.0 85 9.5 86 4.0 87 4.0 88 4.0 89 3.5 90 3.0 91 1.5 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 480897.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.5186373519401 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14120428576793 38.299929642393785 2 5.554477726324649 5.05664514608001 3 2.362152734860373 3.2256592112400853 4 1.5601983794336811 2.8407241692210548 5 1.1993046318348273 2.7295356305495773 6 0.9321911900063105 2.545924363234244 7 0.8051034299746872 2.565304774187462 8 0.6486505411875959 2.362055100196629 9 0.5346219292134644 2.190173554463605 >10 2.1371493116146243 14.498703526287693 >50 0.07543026623868974 2.361098785909886 >100 0.04164861326060529 3.3850350155729534 >500 0.0027765742173736863 0.783381781139752 >1k 0.003239336586935967 3.910589611954691 >5k 0.0 0.0 >10k+ 0.0018510494782491241 13.245239687568594 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCG 18041 3.751530993123268 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17401 3.618446361694916 No Hit GAATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTC 13921 2.894798678303254 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC 13517 2.810789004714107 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTC 3417 0.7105471649854335 No Hit GAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT 3352 0.6970307571059915 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCGTAT 3040 0.6321519992846701 No Hit GAATGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT 2811 0.584532654601713 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTA 2685 0.5583316177892563 No Hit GCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC 2217 0.4610134810572742 TruSeq Adapter, Index 14 (95% over 23bp) GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCGTATCGT 1043 0.21688636028089175 No Hit GAATGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTT 753 0.15658238666491991 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATG 662 0.13765941563370118 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 630 0.13100518406228362 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTAT 614 0.12767806827657482 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 538 0.11187426829445807 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.10854715250874927 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 498 0.1035564788301861 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.07278065781237979 0.0 2 0.0 0.0 0.0 0.6473319650569664 0.0 3 0.0 0.0 0.0 0.7377879254809242 0.0 4 0.0 0.0 0.0 1.6186418297473264 0.0 5 0.0 0.0 0.0 5.18198283624144 0.0 6 0.0 0.0 0.0 5.449191822781177 0.0 7 0.0 0.0 0.0 6.207566277186175 0.0 8 0.0 0.0 0.0 7.124394620885553 0.0 9 0.0 0.0 0.0 7.2608063680996136 0.0 10 0.0 0.0 0.0 11.773623041940374 0.0 11 0.0 0.0 0.0 12.006105257466775 0.0 12 0.0 0.0 0.0 15.528689095585957 0.0 13 0.0 0.0 0.0 15.821683229464938 0.0 14 0.0 0.0 0.0 16.016111558192296 0.0 15 0.0 0.0 0.0 16.771990675758012 0.0 16 0.0 0.0 0.0 17.125912617462784 0.0 17 0.0 0.0 0.0 17.28665389885984 0.0 18 0.0 0.0 0.0 17.404350619779287 0.0 19 0.0 0.0 0.0 18.30059243455459 0.0 20 0.0 0.0 0.0 18.490030089603387 0.0 21 0.0 0.0 0.0 18.632888123652258 0.0 22 0.0 0.0 0.0 18.91922802595982 0.0 23 0.0 0.0 0.0 19.057719220539948 0.0 24 0.0 0.0 0.0 19.189140294075447 0.0 25 0.0 0.0 0.0 19.298519225530622 0.0 26 0.0 0.0 0.0 19.445120264838415 0.0 27 0.0 0.0 0.0 19.673443585632683 0.0 28 0.0 0.0 0.0 19.77762389867269 0.0 29 0.0 0.0 0.0 19.9063416906323 0.0 30 0.0 0.0 0.0 20.031316477332982 0.0 31 0.0 0.0 0.0 20.153380037721174 0.0 32 0.0 0.0 0.0 20.284801111256673 0.0 33 0.0 0.0 0.0 20.410191787430573 0.0 34 0.0 0.0 0.0 20.597757939849906 0.0 35 0.0 0.0 0.0 20.755795939671074 0.0 36 0.0 0.0 0.0 20.89179179741192 0.0 37 0.0 0.0 0.0 21.042343786715243 0.0 38 0.0 0.0 0.0 21.211194912839964 0.0 39 0.0 0.0 0.0 21.408118578406604 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGAT 30 2.1628584E-6 45.000004 5 CACTAGG 30 2.1628584E-6 45.000004 29 AGGCACG 30 2.1628584E-6 45.000004 10 CTATCGT 55 1.8189894E-12 45.0 41 GCAACGA 20 7.02919E-4 45.0 9 GCACGGG 25 3.8874103E-5 45.0 3 GTCTAGG 20 7.02919E-4 45.0 2 CCTATCG 55 1.8189894E-12 45.0 40 CGTTTTT 14780 0.0 44.512856 1 CACCGGT 190 0.0 43.81579 16 GATACCT 1970 0.0 43.743656 5 CGATGAA 625 0.0 43.56 19 TGATACC 2060 0.0 42.269417 4 CCGATGA 640 0.0 42.1875 18 ATACCTG 2050 0.0 42.036587 6 ACGGCTG 1900 0.0 41.92105 8 CCACCGG 215 0.0 41.860466 15 CGGCTGT 1915 0.0 41.59269 9 TACGGCT 1930 0.0 41.15285 7 TGATCTG 460 0.0 41.086956 4 >>END_MODULE