##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933399.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	291846
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.395318078712744	31.0	26.0	31.0	16.0	33.0
2	28.281686231779773	31.0	28.0	33.0	16.0	34.0
3	28.404110386984918	31.0	28.0	33.0	16.0	34.0
4	31.87886762196501	35.0	28.0	37.0	19.0	37.0
5	33.68585486866361	35.0	33.0	37.0	28.0	37.0
6	32.34669997190299	35.0	32.0	37.0	17.0	37.0
7	32.50547549049841	35.0	32.0	37.0	26.0	37.0
8	32.66844157535139	35.0	32.0	37.0	25.0	37.0
9	33.32346511516348	35.0	32.0	39.0	25.0	39.0
10	32.573281799305114	37.0	30.0	39.0	17.0	39.0
11	33.28927242449785	37.0	31.0	39.0	17.0	39.0
12	33.93566127341132	37.0	31.0	39.0	25.0	39.0
13	34.082742268182535	37.0	32.0	39.0	26.0	39.0
14	34.48883657819535	37.0	32.0	40.0	25.0	41.0
15	34.77312349663864	37.0	32.0	40.0	25.0	41.0
16	34.95361252167239	37.0	32.0	40.0	26.0	41.0
17	32.95189928935123	36.0	30.0	40.0	19.0	41.0
18	33.421160475045056	36.0	31.0	39.0	24.0	39.0
19	33.03436744036238	36.0	31.0	37.0	23.0	39.0
20	32.16164346950104	34.0	30.0	35.0	24.0	39.0
21	32.64080713801114	35.0	31.0	36.0	24.0	39.0
22	32.471406152559915	34.0	30.0	37.0	24.0	39.0
23	33.407817821727896	35.0	32.0	37.0	26.0	39.0
24	31.96052370085593	35.0	31.0	36.0	21.0	39.0
25	31.316982244060224	34.0	30.0	36.0	18.0	39.0
26	29.91654845363651	33.0	25.0	36.0	18.0	39.0
27	31.960129657422065	34.0	30.0	37.0	22.0	39.0
28	31.88516889044222	35.0	30.0	37.0	21.0	39.0
29	31.893049759119535	35.0	30.0	37.0	21.0	39.0
30	31.210354090856136	34.0	29.0	37.0	18.0	39.0
31	31.455147577832143	34.0	30.0	36.0	19.0	39.0
32	31.21465773044688	35.0	30.0	37.0	16.0	39.0
33	30.713855937720577	35.0	28.0	37.0	14.0	40.0
34	29.593234102917293	35.0	24.0	37.0	8.0	39.0
35	28.904658621327687	34.0	22.0	37.0	7.0	40.0
36	28.035158953694758	33.0	18.0	37.0	7.0	40.0
37	28.018564585432042	34.0	18.0	37.0	7.0	40.0
38	27.842156479787285	33.0	18.0	37.0	7.0	40.0
39	27.30639446831548	33.0	18.0	37.0	7.0	39.0
40	27.044146570451538	33.0	16.0	37.0	7.0	39.0
41	26.343688109482397	31.0	15.0	36.0	7.0	39.0
42	26.792452183685917	33.0	15.0	36.0	7.0	39.0
43	26.84084071736464	33.0	15.0	37.0	7.0	39.0
44	26.77887653077308	33.0	15.0	37.0	7.0	39.0
45	26.911429315460893	33.0	15.0	37.0	7.0	40.0
46	26.610558308148818	33.0	15.0	37.0	7.0	40.0
47	26.574316591627092	33.0	15.0	37.0	7.0	40.0
48	26.40103342173612	33.0	14.0	36.0	7.0	39.0
49	26.314566586487395	32.0	13.0	36.0	7.0	39.0
50	26.478677110530896	33.0	15.0	36.0	7.0	39.0
51	24.825983566675575	29.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	14.0
11	18.0
12	33.0
13	45.0
14	58.0
15	147.0
16	304.0
17	672.0
18	1300.0
19	1990.0
20	2980.0
21	4264.0
22	5958.0
23	8158.0
24	11003.0
25	15090.0
26	18910.0
27	20488.0
28	19246.0
29	18416.0
30	18938.0
31	20222.0
32	22015.0
33	23479.0
34	24688.0
35	26096.0
36	16961.0
37	8499.0
38	1802.0
39	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.509474174735992	2.03977440156795	25.664220170912056	49.786531252784
2	47.789587659244944	15.585274425553203	25.940735867546582	10.68440204765527
3	9.310047079624185	15.314926365274836	64.49188955819164	10.883136996909329
4	8.760099504533212	2.17546240140348	76.13844287740795	12.925995216655359
5	19.51919848139087	2.4153149263652747	64.15335485153129	13.912131740712569
6	10.829684148489271	13.199084448647575	66.46621848509146	9.505012917771701
7	40.59709572856918	1.0937275138257847	55.73110476072998	2.5780719968750643
8	40.318866799613495	10.5966845528121	45.41950206615818	3.6649465814162263
9	37.14972965193972	2.1648403610123146	44.91889558191649	15.766534405131475
10	23.54666502196364	13.728130589420449	50.01062204039116	12.714582348224749
11	19.415033956264605	11.811023622047244	56.68948692118446	12.08445550050369
12	15.44307614289728	10.503142068077	58.11112710127945	15.942654687746277
13	14.228394427197905	11.596526935438552	64.32193691193301	9.853141725430534
14	11.937460167348533	15.891257718111607	56.907752718899694	15.263529395640166
15	8.815265585274426	11.816848611939173	65.3718056783372	13.996080124449195
16	10.452430391370791	12.527840025218781	55.976096982655235	21.043632600755195
17	11.200770269251592	13.76513640755741	60.76012691625036	14.273966406940646
18	10.938303077650541	12.554909095893038	59.312788251338034	17.193999575118387
19	11.174729137970026	15.768590283916861	55.74618120515614	17.31049937295697
20	13.413581135256264	14.071804993044276	61.53930497591196	10.975308895787505
21	13.44818842814361	18.961370037622583	55.58239619525366	12.008045338980148
22	11.762710470590655	12.025520308655935	58.77654653481631	17.435222685937106
23	12.75398669161133	18.259287432412986	56.570931244560484	12.4157946314152
24	13.078815539702445	13.727102650027753	55.47343461962816	17.72064719064164
25	10.630606552770983	20.69893025773867	53.8924638336657	14.777999355824647
26	11.442336026534543	12.984930408503114	59.53413786723135	16.038595697730994
27	12.946554004509228	14.909232951625173	54.70761977207157	17.436593271794028
28	9.286747120056468	15.718906546603344	60.1652926543451	14.829053678995086
29	14.162948952529758	12.230422894266155	57.22058894074272	16.386039212461366
30	10.909863421119358	17.909445392432996	55.707119508233795	15.473571678213851
31	14.0786579223289	15.346792486448333	52.28819308813553	18.286356503087244
32	15.270724971389022	17.09977179745482	51.63271040206136	15.996792829094797
33	13.136722792157506	15.356043940982572	48.74899775909212	22.758235507767797
34	17.107310019667906	17.436593271794028	50.89464991810749	14.561446790430569
35	12.397291722346719	19.120357997025828	47.4534514778342	21.028898802793254
36	14.575495295464048	23.526448880574	45.14401430891635	16.754041515045607
37	14.441863174413902	19.248850421112504	44.92814703645073	21.38113936802286
38	15.627419940653633	22.115088094405955	42.00091829252414	20.256573672416277
39	19.791945066918853	19.539071976316276	42.294908958834455	18.374073997930417
40	15.506465738780042	18.738992482336574	46.83531725636123	18.919224522522153
41	17.543841615098373	24.934725848563968	41.06103904113814	16.460393495199526
42	14.934588789978276	20.162688541216944	48.140457638617626	16.762265030187155
43	16.38124216196213	21.533274398141486	41.913543444145205	20.171939995751185
44	18.92470686594985	19.919066905148604	41.04699053610466	20.109235692796886
45	15.455411415609602	20.703384661773676	42.603290776642474	21.237913145974247
46	18.979872946691064	22.352199447653902	39.83162352747682	18.836304078178216
47	13.941256690172214	19.10870801724197	47.36950309409757	19.580532198488243
48	18.475154704878598	20.635883308320143	39.700047285212065	21.188914701589194
49	15.091178224131903	18.222281614276024	45.48015049032709	21.206389671264983
50	16.1828498591723	18.568354543149468	42.839031544033496	22.409764053644732
51	16.997320504649714	17.801169109735955	41.88853025225633	23.312980133358003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	297.0
2	538.0
3	18914.0
4	37290.0
5	25153.5
6	13017.0
7	12452.0
8	11887.0
9	11584.0
10	11281.0
11	10412.0
12	9543.0
13	8783.5
14	8024.0
15	7264.5
16	6505.0
17	5855.5
18	5206.0
19	4765.0
20	4324.0
21	3910.5
22	3497.0
23	3306.0
24	3115.0
25	2906.0
26	2661.0
27	2625.0
28	2774.5
29	2924.0
30	3076.5
31	3229.0
32	3423.0
33	3617.0
34	4145.5
35	4674.0
36	4756.5
37	4839.0
38	5244.0
39	5649.0
40	5910.5
41	6172.0
42	6624.0
43	7076.0
44	8280.0
45	9484.0
46	21676.5
47	33869.0
48	26648.5
49	19428.0
50	17576.0
51	15724.0
52	13324.5
53	10925.0
54	9956.0
55	8987.0
56	8460.5
57	7934.0
58	7250.0
59	6566.0
60	6171.0
61	5776.0
62	5130.5
63	4485.0
64	4089.0
65	3693.0
66	3041.0
67	2389.0
68	2093.0
69	1797.0
70	1433.5
71	1070.0
72	937.5
73	805.0
74	651.5
75	409.5
76	321.0
77	238.5
78	156.0
79	114.0
80	72.0
81	63.5
82	55.0
83	36.0
84	17.0
85	10.5
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	291846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.541086622542174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9542028335977	40.266871454496
2	6.826653781465353	6.627463850964296
3	3.0898104410642526	4.499482688008054
4	1.8820980010603807	3.6543632840634164
5	1.3339688960893699	3.237614986842553
6	0.9714220344392956	2.8292328672458398
7	0.6477097000410983	2.2008372859169034
8	0.5135854637722437	1.9943997188037583
9	0.3846135022785106	1.6802601597270455
>10	1.2734354824444345	9.522027252905973
>50	0.07264526803624248	2.275833615259349
>100	0.042020302099395154	3.855399738321777
>500	0.0028488340406369597	1.1315211032574584
>1k	0.0035610425507961994	4.474573064302256
>5k	7.122085101592399E-4	2.8054670800288037
>10k+	7.122085101592399E-4	8.94465184985647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25873	8.865291969052173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCG	8115	2.7805760572356655	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGC	4932	1.689932361587961	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTC	4079	1.3976549275988022	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCGTAT	1489	0.5102005852401609	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT	1339	0.45880361560549054	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTC	1104	0.3782816965111737	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	960	0.32894060566189015	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTA	919	0.3148921006284136	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT	757	0.25938337342296963	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCACCGTATCGTATGCCG	637	0.21826579771523336	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	426	0.1459673937624638	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCGTATCGT	421	0.1442541614413081	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	411	0.14082769679899673	No Hit
GAATCTTTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTC	388	0.1329468281216806	No Hit
GAATGACTCTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT	336	0.11512921198166155	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCC	300	0.10279393926934068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.426464642311356E-4	0.0	0.0	0.09217189887817548	0.0
2	3.426464642311356E-4	0.0	0.0	0.6023724841183364	0.0
3	3.426464642311356E-4	0.0	0.0	0.7699266051273617	0.0
4	3.426464642311356E-4	0.0	0.0	1.5491046647889641	0.0
5	3.426464642311356E-4	0.0	0.0	3.510070379583753	0.0
6	3.426464642311356E-4	0.0	0.0	3.82290660142678	0.0
7	3.426464642311356E-4	0.0	0.0	4.289248439245355	0.0
8	3.426464642311356E-4	0.0	0.0	4.949185529354523	0.0
9	3.426464642311356E-4	0.0	0.0	5.14757783214435	0.0
10	3.426464642311356E-4	0.0	0.0	8.95540798914496	0.0
11	3.426464642311356E-4	0.0	0.0	9.530368756124805	0.0
12	3.426464642311356E-4	0.0	0.0	12.055673197508275	0.0
13	3.426464642311356E-4	0.0	0.0	12.333216833535495	0.0
14	3.426464642311356E-4	0.0	0.0	12.481240106083344	0.0
15	3.426464642311356E-4	0.0	0.0	13.03153032763855	0.0
16	3.426464642311356E-4	0.0	0.0	13.365610630263907	0.0
17	3.426464642311356E-4	0.0	0.0	13.623623417829952	0.0
18	3.426464642311356E-4	0.0	0.0	13.865874468041364	0.0
19	3.426464642311356E-4	0.0	0.0	14.698162729658792	0.0
20	3.426464642311356E-4	0.0	0.0	14.985643113148715	0.0
21	3.426464642311356E-4	0.0	0.0	15.265242627961323	0.0
22	3.426464642311356E-4	0.0	0.0	15.700060991070632	0.0
23	3.426464642311356E-4	0.0	0.0	15.960472303886297	0.0
24	3.426464642311356E-4	0.0	0.0	16.159549899604585	0.0
25	3.426464642311356E-4	0.0	0.0	16.35040398018133	0.0
26	3.426464642311356E-4	0.0	0.0	16.521727212296895	0.0
27	3.426464642311356E-4	0.0	0.0	16.722860686800573	0.0
28	3.426464642311356E-4	0.0	0.0	16.874310423990735	0.0
29	3.426464642311356E-4	0.0	0.0	17.043235130856683	0.0
30	3.426464642311356E-4	0.0	0.0	17.24025684778959	0.0
31	3.426464642311356E-4	0.0	0.0	17.396160989014753	0.0
32	3.426464642311356E-4	0.0	0.0	17.579476847378412	0.0
33	3.426464642311356E-4	0.0	0.0	17.738464806781657	0.0
34	3.426464642311356E-4	0.0	0.0	17.9193821398957	0.0
35	3.426464642311356E-4	0.0	0.0	18.09207595786819	0.0
36	3.426464642311356E-4	0.0	0.0	18.249350684950283	0.0
37	3.426464642311356E-4	0.0	0.0	18.411079816067378	0.0
38	3.426464642311356E-4	0.0	0.0	18.60981476532144	0.0
39	3.426464642311356E-4	0.0	0.0	18.83047908828629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2077726E-7	45.000004	2
ACCGCCT	30	2.1599153E-6	45.000004	14
GCGAGAC	20	7.024626E-4	45.0	21
TCCGACC	20	7.024626E-4	45.0	35
GTCAAGC	20	7.024626E-4	45.0	16
TCAAGCG	20	7.024626E-4	45.0	17
CAAAGTG	20	7.024626E-4	45.0	33
GTCGGGA	25	3.8836304E-5	44.999996	4
CGTTTTT	11995	0.0	44.606087	1
CGATGAA	140	0.0	43.39286	19
GATACCT	1105	0.0	43.16742	5
ACGGCTG	715	0.0	43.11189	8
AATGCTA	95	0.0	42.63158	2
TGATACC	1115	0.0	42.578476	4
AATGATC	230	0.0	42.065216	2
TGATCCG	70	0.0	41.785717	4
TATACTA	70	0.0	41.785717	44
ATGATAC	2870	0.0	41.707317	3
AATGATA	2855	0.0	41.690014	2
ATACCTG	1140	0.0	41.644737	6
>>END_MODULE