##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933398.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375151
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.352260822975282	31.0	28.0	31.0	25.0	34.0
2	28.438148905374103	31.0	26.0	33.0	16.0	34.0
3	28.43634962988237	31.0	26.0	33.0	16.0	34.0
4	33.025603023849065	35.0	32.0	37.0	28.0	37.0
5	34.212506430743886	35.0	33.0	37.0	30.0	37.0
6	33.44791030811593	35.0	32.0	37.0	28.0	37.0
7	33.533755741021615	35.0	32.0	37.0	29.0	37.0
8	33.366609711822704	35.0	33.0	37.0	28.0	37.0
9	34.62721943963897	37.0	34.0	39.0	28.0	39.0
10	34.54691044406119	37.0	32.0	39.0	27.0	39.0
11	34.67418186276993	37.0	33.0	39.0	27.0	39.0
12	34.76649935625921	37.0	34.0	39.0	27.0	39.0
13	34.82127996460092	37.0	34.0	39.0	27.0	39.0
14	35.613382344709194	38.0	34.0	40.0	27.0	41.0
15	35.85853163126315	38.0	34.0	40.0	27.0	41.0
16	35.84775463746598	38.0	34.0	40.0	27.0	41.0
17	35.15689948847264	37.0	33.0	40.0	27.0	41.0
18	35.01338394406519	37.0	33.0	39.0	27.0	40.0
19	34.322198261500034	37.0	32.0	38.0	27.0	40.0
20	33.659606398490205	35.0	32.0	38.0	26.0	40.0
21	34.159725017392994	35.0	33.0	38.0	27.0	40.0
22	34.766669954231766	35.0	33.0	39.0	29.0	40.0
23	35.057854037440926	35.0	33.0	39.0	30.0	40.0
24	34.631785600998	35.0	33.0	39.0	27.0	40.0
25	33.59783127327396	35.0	33.0	38.0	24.0	40.0
26	33.69630628733497	35.0	32.0	38.0	24.0	40.0
27	34.36317642762514	35.0	33.0	39.0	27.0	40.0
28	34.09265069265442	35.0	33.0	39.0	25.0	40.0
29	34.120865997958155	35.0	33.0	39.0	25.0	40.0
30	33.51016257453665	35.0	33.0	39.0	22.0	40.0
31	33.46852334126792	35.0	33.0	39.0	22.0	40.0
32	32.88261526691919	35.0	32.0	39.0	18.0	40.0
33	32.418359540558335	35.0	31.0	39.0	15.0	40.0
34	31.8013573201191	35.0	30.0	39.0	10.0	40.0
35	31.511148844065456	35.0	30.0	40.0	9.0	41.0
36	30.919091245924974	35.0	27.0	39.0	8.0	40.0
37	30.841485161974777	35.0	27.0	39.0	8.0	40.0
38	30.64098989473572	35.0	25.0	39.0	8.0	40.0
39	30.22005272543589	35.0	24.0	39.0	7.0	40.0
40	30.162092064262122	35.0	23.0	39.0	7.0	40.0
41	29.54313862951185	35.0	22.0	39.0	7.0	40.0
42	29.74838664964241	35.0	23.0	39.0	7.0	40.0
43	29.655714632241416	35.0	22.0	39.0	7.0	40.0
44	29.813483104136733	35.0	23.0	39.0	7.0	40.0
45	29.915919189872877	35.0	23.0	39.0	7.0	40.0
46	29.665332092943906	35.0	23.0	39.0	7.0	40.0
47	29.595752110483513	35.0	23.0	39.0	7.0	40.0
48	29.471551455280675	35.0	23.0	38.0	7.0	40.0
49	29.697934964854152	35.0	23.0	39.0	7.0	40.0
50	29.649583767602913	35.0	23.0	39.0	7.0	40.0
51	28.389752393036403	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	5.0
9	15.0
10	29.0
11	32.0
12	36.0
13	49.0
14	67.0
15	96.0
16	185.0
17	383.0
18	799.0
19	1329.0
20	2106.0
21	3304.0
22	4643.0
23	6425.0
24	9324.0
25	13755.0
26	17357.0
27	18382.0
28	17918.0
29	16513.0
30	17497.0
31	19730.0
32	23068.0
33	27238.0
34	30893.0
35	35457.0
36	38429.0
37	37912.0
38	28232.0
39	3941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.677383240348554	2.331327918624767	31.368169083915543	36.62311975711114
2	36.20115633438269	18.754848047852732	31.398023729111745	13.645971888652836
3	12.48084104800467	19.04593083851569	55.04876702980933	13.424461083670309
4	11.238408001044913	2.92522211056348	70.05685710553884	15.779512782852773
5	24.158272269033002	3.305069158818716	55.563759659443804	16.972898912704483
6	13.482304458737948	16.97449826869714	58.30558894951633	11.237608323048585
7	51.88364152034781	1.2986770660347433	44.02600552844055	2.791675885176902
8	50.770756308793	13.126181191040406	32.70363133778132	3.3994311623852793
9	47.9556232023905	2.7586225279953935	31.699235774394847	17.58651849521926
10	30.63166564929855	18.258781130798	37.74986605393561	13.359687165967838
11	27.35938328832923	14.694349741837287	44.21153082358837	13.73473614624511
12	20.47682133327647	13.650236837966577	46.5178021649949	19.35513966376206
13	18.900389443184213	14.928921954093152	54.20297426902767	11.967714333694964
14	16.13616916921453	20.953962537751465	45.97748639881008	16.932381894223923
15	12.566673152943746	14.24919565721536	56.867234793456504	16.31689639638439
16	14.968639294577383	16.861743671215056	44.668146959490976	23.50147007471658
17	15.623042454904827	17.58891752920824	50.57136992837551	16.216670087511428
18	16.425386044552724	15.546273367257452	48.512465647166074	19.515874941023746
19	15.869343277773485	18.838814237467048	44.990950310674904	20.30089217408457
20	18.23399111291187	18.299564708610667	50.69585313647038	12.770591042007084
21	17.594248715850416	24.03352250160602	45.14528816396597	13.226940618577586
22	15.422323277826793	14.160697958955193	50.02598953488062	20.39098922833739
23	15.427387905136866	22.68873067111643	47.549653339588595	14.334228084158113
24	17.159223885848633	17.19734187034021	44.88379345916711	20.759640784644052
25	14.8617490024017	26.798542453572026	42.21740046008141	16.122308083944866
26	15.386870886656306	15.866144565788176	48.05638262992768	20.690601917627834
27	17.84934599667867	18.458167511215485	44.23152277349654	19.460963718609307
28	12.160436730809728	20.778299937891674	49.023193327486794	18.0380700038118
29	18.6495571116697	19.241052269619434	43.86926864116049	18.240121977550373
30	15.116046605233628	21.50520723655275	48.7102526715909	14.668493486622719
31	19.574251434755606	20.23531857838577	41.09838438388809	19.092045602970536
32	24.25450018792433	21.29382568619036	39.87620984616861	14.575464279716702
33	18.09298122622624	18.237456384229283	37.297248308014645	26.372314081529836
34	20.066319961828704	26.945416645564052	37.90980165320097	15.078461739406265
35	20.12416333689634	21.757105805395692	39.35961786054149	18.759112997166476
36	21.375392841815696	29.368707533766404	32.147588571002075	17.108311053415825
37	22.28702575762826	22.56291466636101	38.50182992981493	16.648229646195798
38	20.73191861410472	25.7925475341929	31.294598708253478	22.180935143448906
39	24.681794797294955	25.409768333284465	31.96019736052949	17.94823950889109
40	24.323272495608435	20.94543263912398	34.24567707403152	20.485617791236063
41	23.022996073581037	27.15439916193746	30.76201316269982	19.06059160178168
42	23.041655226828663	21.434835572875986	37.53581891025214	17.987690290043208
43	24.661802847386785	21.28929417754451	32.03590021084843	22.013002764220275
44	23.29382035500372	21.29595816084723	34.86142913120317	20.54879235294588
45	19.527070432972323	21.556120068985553	33.707493782503576	25.209315715538544
46	25.278621141886866	23.95115566798436	31.19170680605943	19.578516384069346
47	16.503221369528536	21.386055215100054	42.1246378125075	19.986085602863913
48	20.77510122590637	26.03991459438999	31.40975233972454	21.7752318399791
49	20.18493886461718	20.502144469826817	38.211546817148296	21.101369848407707
50	21.066450575901438	20.614632507976786	34.822778028047374	23.4961388880744
51	20.018872400713313	20.941434249142347	32.97525529720033	26.064438052944016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	542.5
2	1044.0
3	11968.0
4	22892.0
5	16988.0
6	11084.0
7	10394.5
8	9705.0
9	9329.0
10	8953.0
11	8513.0
12	8073.0
13	7529.5
14	6986.0
15	6478.5
16	5971.0
17	5563.0
18	5155.0
19	4677.0
20	4199.0
21	4060.5
22	3922.0
23	3771.5
24	3621.0
25	3653.0
26	3825.5
27	3966.0
28	4422.0
29	4878.0
30	5138.0
31	5398.0
32	5758.0
33	6118.0
34	6775.0
35	7432.0
36	7541.5
37	7651.0
38	8195.5
39	8740.0
40	9167.5
41	9595.0
42	10410.5
43	11226.0
44	12503.5
45	13781.0
46	37256.0
47	60731.0
48	41822.5
49	22914.0
50	21613.5
51	20313.0
52	18563.0
53	16813.0
54	16708.0
55	16603.0
56	15960.5
57	15318.0
58	13951.5
59	12585.0
60	11302.0
61	10019.0
62	8797.0
63	7575.0
64	6747.5
65	5920.0
66	4929.5
67	3939.0
68	3437.0
69	2935.0
70	2430.0
71	1925.0
72	1615.5
73	1306.0
74	1111.5
75	755.0
76	593.0
77	474.5
78	356.0
79	244.0
80	132.0
81	113.5
82	95.0
83	61.0
84	27.0
85	18.5
86	10.0
87	6.0
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	375151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.56714383916006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1189970445601	36.963610064178695
2	6.163498844813378	5.617060768282161
3	3.084485300751645	4.216535561073751
4	2.253107802386904	4.106707492659914
5	1.6989464730757697	3.870806915683863
6	1.3135114315665026	3.591177860194312
7	1.0007791462192395	3.192185311490271
8	0.799645432258896	2.9150046745654743
9	0.5735997622346736	2.352357258466984
>10	1.8950166109644802	12.99519970283775
>50	0.06010802047412481	1.863560652444261
>100	0.030643304555436176	2.373757372854073
>500	0.0017678829551213179	0.5042891794366458
>1k	0.0035357659102426358	3.359779666193457
>5k	5.892943183737726E-4	2.6237537658548806
>10k+	0.0017678829551213179	9.454213753783506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	13070	3.4839304706638123	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11070	2.950811806445938	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	11068	2.95027868778172	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	9771	2.604551234036428	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGCAGGT	2424	0.6461398210320644	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC	2214	0.5901623612891875	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	2029	0.540848884849034	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	2003	0.5339183422142018	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	1942	0.5176582229555565	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA	1900	0.5064627310069812	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT	689	0.1836593798230579	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGT	675	0.1799275491735328	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTAT	514	0.13701149670399385	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATG	425	0.11328771614629843	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07570285031893824	0.0
2	0.0	0.0	0.0	0.733038163299578	0.0
3	0.0	0.0	0.0	0.831398556847776	0.0
4	0.0	0.0	0.0	1.4903332258210693	0.0
5	0.0	0.0	0.0	4.4941903393566855	0.0
6	0.0	0.0	0.0	4.774877316067397	0.0
7	0.0	0.0	0.0	5.448206188974573	0.0
8	0.0	0.0	0.0	6.210832438138243	0.0
9	0.0	0.0	0.0	6.335049086901008	0.0
10	0.0	0.0	0.0	10.445660547352933	0.0
11	0.0	0.0	0.0	10.648245639755725	0.0
12	0.0	0.0	0.0	14.200948418103643	0.0
13	0.0	0.0	0.0	14.445649884979648	0.0
14	0.0	0.0	0.0	14.658897350666798	0.0
15	0.0	0.0	0.0	15.329827189584993	0.0
16	0.0	0.0	0.0	15.623042454904825	0.0
17	0.0	0.0	0.0	15.789108918808694	0.0
18	0.0	0.0	0.0	15.916257720224657	0.0
19	0.0	0.0	0.0	16.83162246668675	0.0
20	0.0	0.0	0.0	17.031275406436343	0.0
21	0.0	0.0	0.0	17.15709141119176	0.0
22	0.0	0.0	0.0	17.396728250757693	0.0
23	0.0	0.0	0.0	17.51588027221039	0.0
24	0.0	0.0	0.0	17.634765734330976	0.0
25	0.0	0.0	0.0	17.727794941236994	0.0
26	0.0	0.0	0.0	17.84668040335758	0.0
27	0.0	0.0	0.0	18.021543325221046	0.0
28	0.0	0.0	0.0	18.119370600105025	0.0
29	0.0	0.0	0.0	18.242254452207245	0.0
30	0.0	0.0	0.0	18.382731220228656	0.0
31	0.0	0.0	0.0	18.49681861437128	0.0
32	0.0	0.0	0.0	18.635962585732145	0.0
33	0.0	0.0	0.0	18.759112997166476	0.0
34	0.0	0.0	0.0	18.953967868938108	0.0
35	0.0	0.0	0.0	19.102441416922787	0.0
36	0.0	0.0	0.0	19.24158538828365	0.0
37	0.0	0.0	0.0	19.378596884987644	0.0
38	0.0	0.0	0.0	19.559057552825397	0.0
39	0.0	0.0	0.0	19.887458649983607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGGG	20	7.027206E-4	45.0	3
CGTTCAT	35	1.2090095E-7	45.0	17
CGTTAGG	25	3.885765E-5	45.0	2
TAGGGCG	45	3.8380676E-10	45.0	5
GCTACGA	30	2.1615779E-6	44.999996	10
CGTTTTT	9460	0.0	44.001057	1
TGATCTG	220	0.0	42.954548	4
GATACCT	1400	0.0	42.910713	5
TGATACC	1400	0.0	42.75	4
TACGGCT	1335	0.0	42.30337	7
ACCCGTC	80	0.0	42.1875	17
CACCCGT	75	0.0	42.0	16
ACGGCTG	1345	0.0	41.988846	8
CGGCTGT	1365	0.0	41.20879	9
ATACCTG	1475	0.0	41.186443	6
AATGATC	255	0.0	40.588234	2
TGCGTTG	50	1.0768417E-9	40.5	1
CCGATGA	385	0.0	40.324673	18
GAATCTG	1180	0.0	40.23305	1
CACGACC	90	0.0	40.0	27
>>END_MODULE